Article ; Online: Treatment of dental plaque biofilms using photodynamic therapy: a randomised controlled study.
2021 Volume 22, Issue 5, Page(s) 791–800
Abstract: Introduction: Photodynamic therapy (PDT) is a treatment modality involving a dye that is activated by exposure to light of a specific wavelength in the presence of oxygen to form oxygen species causing localised damage to microorganisms.: Aim: To ... ...
Abstract | Introduction: Photodynamic therapy (PDT) is a treatment modality involving a dye that is activated by exposure to light of a specific wavelength in the presence of oxygen to form oxygen species causing localised damage to microorganisms. Aim: To determine the most effective bactericidal incubation and irradiation times of erythrosine-based PDT on in vivo-formed dental plaque biofilms. Methods: A randomised controlled study; 18-healthy adult participants wearing intraoral appliances with human enamel slabs to collect dental plaque samples in two separate periods of two weeks each for use in arm-1 and arm-2. These accumulated dental plaque samples were treated with PDT under different experimental conditions. Incubation times with photosensitiser (erythrosine) of 15 min and 2 min were used in arm-1 and arm-2, respectively, followed by light irradiation for either 15 min (continuous) or as a fractionated dose (5 × 30 sec). Following treatment, percentage reductions of total bacterial counts were compared between the different groups. In addition, confocal laser scanning microscopy (CLSM) and LIVE/DEAD® BacLight™ Bacterial Viability Kit were used to visualise the effect of PDT on in vivo-formed biofilms. Results: Significant reductions in the percentage of total bacterial counts (~93-95%) of in vivo-formed biofilms were found when using either 2 min or 15min incubation times and applying 15 min continuous light. Although when applying fractionated light, there was more cell death when 15 min incubation time was used (~ 91%) compared with the 2 min incubation time (~ 64%). CLSM results supported these findings. Conclusion: Improving the clinical usefulness of PDT by reducing its overall treatment time seems to be promising and effective in killing in vivo-formed dental plaque biofilms. |
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MeSH term(s) | Biofilms ; Dental Plaque ; Erythrosine ; Humans ; Photochemotherapy ; Photosensitizing Agents/therapeutic use |
Chemical Substances | Photosensitizing Agents ; Erythrosine (PN2ZH5LOQY) |
Language | English |
Publishing date | 2021-06-05 |
Publishing country | England |
Document type | Journal Article ; Randomized Controlled Trial |
ZDB-ID | 2472851-2 |
ISSN | 1996-9805 ; 1818-6300 |
ISSN (online) | 1996-9805 |
ISSN | 1818-6300 |
DOI | 10.1007/s40368-021-00637-y |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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