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  1. Article: Genetic and epigenetic factors associated with depression: An updated overview.

    Alshaya, Dalal S

    Saudi journal of biological sciences

    2022  Volume 29, Issue 8, Page(s) 103311

    Abstract: Depression is a complex psychiatric disturbance involving many environmental, genetic, and epigenetic factors. Until now, genetic, and non-genetic studies are still on the way to understanding the complex mechanism of this disease, and there are still ... ...

    Abstract Depression is a complex psychiatric disturbance involving many environmental, genetic, and epigenetic factors. Until now, genetic, and non-genetic studies are still on the way to understanding the complex mechanism of this disease, and there are still many questions that have not yet been answered. Depression includes a large spectrum of heterogeneous symptoms correlated to the deficit of a range of psychological, cognitive, and emotional processes, and it affects various age groups. It is classified into several types according to the severity of symptoms, time of occurrence, and time. Following the World Health Organization (WHO), depression attacks near 350 million persons globally. Several factors overlap in causing depression, including genetic and epigenetic factors, environmental conditions, various stresses, lack of some nutrients to which people are exposed, and excessive stress and abuse in childhood. This study included conducting surveys on depression and new treatment trends based on epigenetic factors associated with the occurrence of the disease. Epigenetic factors provide a completely novel dimension to therapeutic approaches as most diseases are not monogenic, and it is likely that the environment has a significant contribution. Epigenetic inheritance is included in many mental and psychiatric disorders such as depression. In general, epigenetic modifications could be summarized in 3 major points: DNA methylation, histone modification, and non-mediated regulation of RNA (ncRNA). This study also describes some genes associated with one of the depressive disorders using bioinformatics tools and gene bank and had the genes:
    Language English
    Publishing date 2022-05-20
    Publishing country Saudi Arabia
    Document type Journal Article ; Review
    ZDB-ID 2515206-3
    ISSN 2213-7106 ; 1319-562X
    ISSN (online) 2213-7106
    ISSN 1319-562X
    DOI 10.1016/j.sjbs.2022.103311
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  2. Article ; Online: A comparison of logistic regression and classification tree to assess brucellosis associated risk factors in dairy cattle.

    Megahed, Ameer / Kandeel, Sahar / Alshaya, Dalal S / Attia, Kotb A / AlKahtani, Muneera D F / Albohairy, Fatima M / Selim, Abdelfattah

    Preventive veterinary medicine

    2022  Volume 203, Page(s) 105664

    Abstract: Machine learning approaches have been increasingly utilized in the field of medicine. Brucellosis is one of the most common contagious zoonotic diseases with significant impacts on livestock health, reproduction, production, and public health worldwide. ... ...

    Abstract Machine learning approaches have been increasingly utilized in the field of medicine. Brucellosis is one of the most common contagious zoonotic diseases with significant impacts on livestock health, reproduction, production, and public health worldwide. Therefore, our objective was to determine the seroprevalence and compare the logistic regression and Classification and Regression Tree (CART) data-mining analysis to assess risk factors associated with Brucella infection in the densest cattle populated Egyptian governorates. A cross-sectional study was conducted on 400 animals (383 cows, 17 bulls) distributed over four Governorates in Egypt's Nile Delta in 2019. The randomly selected animals from studied geographical areas were serologically tested for Brucella using iELISA, and the animals' information was obtained from the farm records or animal owners. Eight supposed risk factors (geographic location, gender, herd size, age, history of abortion, shared equipment, and disinfection post-calving) were evaluated using multiple stepwise logistic regression and CART machine-learning techniques. A total of 84 (21.0%; 95% CI 17.1-25.3) serum samples were serologically positive for Brucella. The highest seroprevalence of Brucella infection was reported among animals raised in herd size > 100 animals (65.5%), with no disinfection post-calving (61.7%), with a history of abortion (59.6%), and with shared equipment without thorough cleaning and disinfection (57.1%). The multiple stepwise logistic regression modeling identified herd size, history of abortion, and disinfection post-calving as important risk factors. However, CART modeling identified herd size, disinfection post-calving, history of abortion, and shared equipment as the most potential risk factors for Brucella infection. Comparing the two models, CART model showed a higher area under the receiver operating characteristic curve (AUROC = 0.98; 95% CI 0.95 - 1.00) than the binary logistic regression (AUROC = 0.89; 95% CI 0.73 - 0.92). Our findings strongly imply that Brucella infection is most likely to spread among animals raised in large herds (>100 animals) with a history of abortions and bad hygienic measures post-calving. The CART data-mining modeling provides an accurate technique to identify risk factors of Brucella infection in cattle.
    MeSH term(s) Animal Husbandry/methods ; Animals ; Antibodies, Bacterial ; Brucella ; Brucellosis/epidemiology ; Brucellosis/veterinary ; Brucellosis, Bovine/epidemiology ; Cattle ; Cattle Diseases/epidemiology ; Cross-Sectional Studies ; Female ; Logistic Models ; Male ; Pregnancy ; Risk Factors ; Seroepidemiologic Studies
    Chemical Substances Antibodies, Bacterial
    Language English
    Publishing date 2022-05-09
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 43399-8
    ISSN 1873-1716 ; 0167-5877
    ISSN (online) 1873-1716
    ISSN 0167-5877
    DOI 10.1016/j.prevetmed.2022.105664
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  3. Article: A comparison of logistic regression and classification tree to assess brucellosis associated risk factors in dairy cattle

    Megahed, Ameer / Kandeel, Sahar / Alshaya, Dalal S. / Attia, Kotb A. / AlKahtani, Muneera D.F. / Albohairy, Fatima M. / Selim, Abdelfattah

    Preventive veterinary medicine. 2022 June, v. 203

    2022  

    Abstract: Machine learning approaches have been increasingly utilized in the field of medicine. Brucellosis is one of the most common contagious zoonotic diseases with significant impacts on livestock health, reproduction, production, and public health worldwide. ... ...

    Abstract Machine learning approaches have been increasingly utilized in the field of medicine. Brucellosis is one of the most common contagious zoonotic diseases with significant impacts on livestock health, reproduction, production, and public health worldwide. Therefore, our objective was to determine the seroprevalence and compare the logistic regression and Classification and Regression Tree (CART) data-mining analysis to assess risk factors associated with Brucella infection in the densest cattle populated Egyptian governorates. A cross-sectional study was conducted on 400 animals (383 cows, 17 bulls) distributed over four Governorates in Egypt's Nile Delta in 2019. The randomly selected animals from studied geographical areas were serologically tested for Brucella using iELISA, and the animals' information was obtained from the farm records or animal owners. Eight supposed risk factors (geographic location, gender, herd size, age, history of abortion, shared equipment, and disinfection post-calving) were evaluated using multiple stepwise logistic regression and CART machine-learning techniques. A total of 84 (21.0%; 95% CI 17.1–25.3) serum samples were serologically positive for Brucella. The highest seroprevalence of Brucella infection was reported among animals raised in herd size > 100 animals (65.5%), with no disinfection post-calving (61.7%), with a history of abortion (59.6%), and with shared equipment without thorough cleaning and disinfection (57.1%). The multiple stepwise logistic regression modeling identified herd size, history of abortion, and disinfection post-calving as important risk factors. However, CART modeling identified herd size, disinfection post-calving, history of abortion, and shared equipment as the most potential risk factors for Brucella infection. Comparing the two models, CART model showed a higher area under the receiver operating characteristic curve (AUROC = 0.98; 95% CI 0.95 – 1.00) than the binary logistic regression (AUROC = 0.89; 95% CI 0.73 – 0.92). Our findings strongly imply that Brucella infection is most likely to spread among animals raised in large herds (>100 animals) with a history of abortions and bad hygienic measures post-calving. The CART data-mining modeling provides an accurate technique to identify risk factors of Brucella infection in cattle.
    Keywords Brucella ; artificial intelligence ; blood serum ; brucellosis ; cross-sectional studies ; dairy cattle ; disinfection ; equipment ; farms ; herd size ; hygiene ; medicine ; models ; public health ; regression analysis ; reproduction ; risk assessment ; river deltas ; seroprevalence ; veterinary medicine ; Egypt
    Language English
    Dates of publication 2022-06
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 43399-8
    ISSN 1873-1716 ; 0167-5877
    ISSN (online) 1873-1716
    ISSN 0167-5877
    DOI 10.1016/j.prevetmed.2022.105664
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  4. Article ; Online: SARS-CoV-2 virus associated angiotensin converting enzyme 2 expression modulation in colorectal cancer: Insights from mRNA and protein analysis COVID-19 associated (ACE2) expression in colorectal cancer.

    Alotaibi, Mashael Alhumaidi / Al-Hazani, Tahani Mohamed Ibrahim / Alwaili, Maha Abdulla / Jalal, Areej Saud / Alshaya, Dalal S / Safhi, Fatmah Ahmed / Alamoudi, Muna O / Alarifi, Saud / Saeed Al-Qahtani, Wedad

    Microbial pathogenesis

    2023  Volume 185, Page(s) 106389

    Abstract: The SARS-CoV-2 virus gains entry into human cells by exploiting the angiotensin-converting enzyme 2 (ACE2), a key component known as the spike protein (S), as a point of entry. Initially, SARS-CoV-2 suppresses the natural function of ACE2, leading to a ... ...

    Abstract The SARS-CoV-2 virus gains entry into human cells by exploiting the angiotensin-converting enzyme 2 (ACE2), a key component known as the spike protein (S), as a point of entry. Initially, SARS-CoV-2 suppresses the natural function of ACE2, leading to a gradual decline in cell health. Additionally, individuals with cancer are considered more susceptible to COVID-19. This study investigates the expression patterns of ACE2 in colorectal cancer (CRC) patients with and without a history of COVID-19 infection. RT-PCR was used to analyze samples from both cancerous and adjacent non-affected colorectal tissues of 47 CRC patients, comprising two groups: 24 CRC patients with no history of COVID-19 and 23 CRC patients with a recent history of COVID-19 infection. Epithelial CR cells were isolated from both types of tissues and cultured to evaluate cell adhesion. Immunohistochemistry analyses were conducted to examine ACE2 protein expression using various ACE2 antibodies for both cell types. The study revealed ACE2 mRNA expression in all CRC tissues of patients with and without a history of COVID-19. ACE2 expression was significantly higher in CRC patients without a history of COVID-19. Notably, the non-affected colorectal cancer (NACRC) tissues of patients without a history of COVID-19 also showed ACE2 expression, whereas no ACE2 expression was detected in the biopsies of CRC patients with a positive COVID-19 history. ACE2 antibodies were employed to validate ACE2 protein expression at the mRNA level. COVID-19 appears to downregulate ACE2 expression in both CRC and NACRC tissues of CRC patients with a positive history of COVID-19 infection.
    MeSH term(s) Humans ; COVID-19 ; SARS-CoV-2/genetics ; Angiotensin-Converting Enzyme 2/genetics ; RNA, Messenger/genetics ; Peptidyl-Dipeptidase A/genetics ; Peptidyl-Dipeptidase A/metabolism ; Colorectal Neoplasms
    Chemical Substances Angiotensin-Converting Enzyme 2 (EC 3.4.17.23) ; RNA, Messenger ; Peptidyl-Dipeptidase A (EC 3.4.15.1)
    Language English
    Publishing date 2023-10-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 632772-2
    ISSN 1096-1208 ; 0882-4010
    ISSN (online) 1096-1208
    ISSN 0882-4010
    DOI 10.1016/j.micpath.2023.106389
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  5. Article ; Online: FGFR3 and FGFR4 overexpression in juvenile nasopharyngeal angiofibroma: impact of smoking history and implications for personalized management.

    Safhi, Fatmah Ahmed / Al-Hazani, Tahani Mohamed Ibrahim / Jalal, Areej Saud / Alduwish, Manal Abdullah / Alshaya, Dalal S / Almufareh, Nawaf Abdulrahman / Domiaty, Dalia Mostafa / Alshehri, Eman / Al-Shamrani, Salha M / Abboosh, Tahani Saeed / Alotaibi, Mashael Alhumaidi / Alwaili, Maha Abdulla / Al-Qahtani, Wedad Saeed

    Journal of applied genetics

    2023  Volume 64, Issue 4, Page(s) 749–758

    Abstract: Lifestyle factors, including smoking, have been linked to neoplastic diseases, and reports suggest an association between smoking and overexpression of FGFR (fibroblast growth factor receptor) in certain neoplasms. This study aims to assess the ... ...

    Abstract Lifestyle factors, including smoking, have been linked to neoplastic diseases, and reports suggest an association between smoking and overexpression of FGFR (fibroblast growth factor receptor) in certain neoplasms. This study aims to assess the expression of FGFR3 and FGFR4 genes in patients with and without a history of smoking.A total of 118 participants were recruited, including 83 Juvenile Nasopharyngeal Angiofibroma (JNA) patients and 35 healthy participants, the JNA patients were further stratified as smokers and nonsmokers. Total RNA was extracted from the blood & saliva sample by using TRIzol reagent, and quantified using a Nanodrop, and then subjected to gene expression analysis of FGFR3/4 using RT-PCR. Immunohistochemistry analysis was employed using fresh biopsies of JNA to validate the findings. All experiments were performed in triplicates and analysed using the Chi-Square test (P < 0.05). Smokers exhibited significantly lower total RNA concentrations across all sample types (P < 0.001). The study revealed significant upregulation of both FGFR3/4 genes in JNA patients (P < 0.05). Moreover, FGFR3 expression was significantly higher among smokers 66% (95% CI: 53-79%) compared to non-smokers 22% (95% CI: 18-26%). Immunohistochemistry analysis demonstrated moderate to strong staining intensity for FGFR3 among smokers. The study highlights the overexpression of FGFR3/4 genes in JNA patients, with a stronger association observed among smokers. Furthermore, medical reports indicated higher rates of recurrence and bleeding intensity among smokers. These findings emphasize the potential role of FGFR3 as a key molecular factor in JNA, particularly in the context of smoking.
    MeSH term(s) Humans ; Angiofibroma/genetics ; Angiofibroma/metabolism ; Angiofibroma/pathology ; Nasopharyngeal Neoplasms/genetics ; Nasopharyngeal Neoplasms/metabolism ; Nasopharyngeal Neoplasms/pathology ; Immunohistochemistry ; Smoking/genetics ; RNA ; Receptor, Fibroblast Growth Factor, Type 3/genetics ; Receptor, Fibroblast Growth Factor, Type 4/genetics
    Chemical Substances RNA (63231-63-0) ; FGFR3 protein, human (EC 2.7.10.1) ; Receptor, Fibroblast Growth Factor, Type 3 (EC 2.7.10.1) ; FGFR4 protein, human (EC 2.7.10.1) ; Receptor, Fibroblast Growth Factor, Type 4 (EC 2.7.10.1)
    Language English
    Publishing date 2023-09-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 1235302-4
    ISSN 2190-3883 ; 1234-1983
    ISSN (online) 2190-3883
    ISSN 1234-1983
    DOI 10.1007/s13353-023-00780-w
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  6. Article ; Online: Three cases of colon cancer in four generations of the Saudi family, caused by endogamous germline mutations.

    Alshaya, Dalal S / Al-Hazani, Tahani Mohamed Ibrahim / Alotaibi, Mashael Alhumaidi / Domiaty, Dalia Mostafa / Alshehri, Eman / Abdulla Alwaili, Maha / Alotaibi, Amani Mohammed / Jalal, Areej Saud / AlQahtani, Alaa Ali / AlQassim, Fatima Abdullah / Albasher, Gadah / Saeed Al-Qahtani, Wedad

    Cellular and molecular biology (Noisy-le-Grand, France)

    2023  Volume 69, Issue 8, Page(s) 163–171

    Abstract: Various research pieces of evidence have been published in recent years, establishing the increasing prevalence of early colon cancer among young people. In this background, the current study aimed to analyze the reasons behind colon cancer recurrence ... ...

    Abstract Various research pieces of evidence have been published in recent years, establishing the increasing prevalence of early colon cancer among young people. In this background, the current study aimed to analyze the reasons behind colon cancer recurrence among endogamous consanguineous cases in four generations of a single Saud family. For this study, the authors conducted the whole-exome sequencing analysis to screen for germline mutations in DNA samples from consanguineous cases within the family. After collecting the colon samples, it was analyzed histologically and immunohistochemically with the help of Breast Cancer antibodies (BRCA2 and 1 correspondingly) and H&M staining (hematoxylin and eosin). For this study, 26 at-risk consanguineous cases were considered. Three cases were diagnosed with malignant colon cancer, two with breast cancer, and 17 with germline mutations, yet remain unaffected by cancerous tumors. The rest, four consanguineous cases, are healthy and non-carriers of the mutations. However, as per the exome analysis outcomes, 15 cases inherited germline mutations in nine genes. Nine substitution mutations were present in six of the nine inherited genes in these inherited germline mutations. Furthermore, it also presented six insertion and deletion frameshift mutations in five of nine inherited genes. The immunohistochemical staining process achieved positive staining outcomes for BRCA1 and 2. Therefore, germline mutations inherited from the nine genes of endogamous consanguineous cases of mutation carriers remain the primary reason behind colon cancer recurrence in the same family.
    MeSH term(s) Humans ; Adolescent ; Female ; Germ-Line Mutation/genetics ; Saudi Arabia ; Neoplasm Recurrence, Local ; Colonic Neoplasms/genetics ; Breast Neoplasms
    Language English
    Publishing date 2023-08-31
    Publishing country France
    Document type Journal Article
    ZDB-ID 1161779-2
    ISSN 1165-158X ; 0145-5680
    ISSN (online) 1165-158X
    ISSN 0145-5680
    DOI 10.14715/cmb/2023.69.8.25
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    Zs.A 3812: Show issues Location:
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  7. Article ; Online: Detecting STR profiles from degrading menstrual blood samples and their use as possible evidence in forensic investigations.

    Al-Hazani, Tahani Mohamed Ibrahim / Al-Qahtani, Wedad Saeed / Abboosh, Tahani Saeed / Safhi, Fatmah Ahmed / Alshaya, Dalal S / Jalal, Areej Saud / Al-Shamrani, Salha M / Al-Ghamdi, Nouf Abdallah / Alotaibi, Amani Mohammed / Alotaibi, Mashael Alhumaidi / Alghamdi, Hanan S / Alafari, Hayat Ali / ALMatrafi, Tahani Ahmad / Alshehri, Eman

    Forensic science international

    2023  Volume 343, Page(s) 111562

    Abstract: This research explores DNA consistency and attempts to detect STR profiles from the degrading menstrual blood samples (MBS) as reliable forensic evidence. Peripheral (PBS) and MBS of 30 healthy fertile females were taken on the menstrual cycle's second ... ...

    Abstract This research explores DNA consistency and attempts to detect STR profiles from the degrading menstrual blood samples (MBS) as reliable forensic evidence. Peripheral (PBS) and MBS of 30 healthy fertile females were taken on the menstrual cycle's second day. They were obtained at different time periods (0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 24, and 48 h) at 25 °C. DNA evaluation was fulfilled to analyze DNA profiles. A considerable elevation in the median concentrations of DNA between 0 and 14-h intervals were documented, whereas decreased extents were registered between 16 and 48 h. Moreover, complete STR profiles (24/24) for DNA were discovered in all the intervals (0, 2, and 48 h). Periods of 0-8 h demonstrated the maximum extents of DNA materials. Full STR were discovered in all the intervals (0, 2, and 48 h). Eventually, MBS can be utilized as forensic evidence.
    MeSH term(s) Female ; Humans ; Microsatellite Repeats ; DNA Fingerprinting ; DNA/genetics
    Chemical Substances DNA (9007-49-2)
    Language English
    Publishing date 2023-01-07
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 424042-x
    ISSN 1872-6283 ; 0379-0738
    ISSN (online) 1872-6283
    ISSN 0379-0738
    DOI 10.1016/j.forsciint.2023.111562
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  8. Article ; Online: Genetic characterization of the highlander Tibetan population from Qinghai-Tibet Plateau revealed by X chromosomal STRs.

    Li, Xiao-Na / Adnan, Atif / Hadi, Sibte / Al-Qahtani, Wedad Saeed / Alwaili, Maha Abdullah / Alshaya, Dalal S / Jalal, Areej S / Amer, Sayed A M / Jin, Feng

    publication RETRACTED

    PloS one

    2022  Volume 17, Issue 8, Page(s) e0271769

    Abstract: Tibetans are considered an East Asian ethnic group and primarily live in the high Tibetan plateau, the western Sichuan and Yunnan mountains of central and southern China, and areas throughout the Himalayas and around the Tibetan plateau. These people ... ...

    Abstract Tibetans are considered an East Asian ethnic group and primarily live in the high Tibetan plateau, the western Sichuan and Yunnan mountains of central and southern China, and areas throughout the Himalayas and around the Tibetan plateau. These people exhibit rare molecular machinery that allows them to adapt to hypoxic environments in the Qinghai-Tibet Plateau and make them a potential candidate for providing insights related to medical genetic, molecular medicine and human population studies. In the current study, we have genotyped 549 individuals with Investigator Argus X-12 Kit. For 12 X-STRs, a total of 174 unique alleles were found, among them DXS10134 and DXS10135 were the most polymorphic loci. All of the loci were in Hardy-Weinberg Equilibrium (HWE). The numbers of observed haplotypes in Highlander Tibetans males were 161,112, 96 and 108, respectively, whereas haplotype diversities (HD) were 0.9959, 0.9880, 0.9809 and 0.9873, respectively. The combined discrimination power for males (PDm) was 0.999 999 99701 and for females (PDf) was 0.999 999 999 999 9958. This study represents an extensive report on X chromosomal STR markers variation in the Highlander Tibetans population for forensic applications and population genetic studies.
    MeSH term(s) China ; Chromosomes, Human, X/genetics ; Female ; Gene Frequency ; Genetic Markers ; Genetics, Population ; Haplotypes ; Humans ; Male ; Microsatellite Repeats/genetics ; Tibet/epidemiology
    Chemical Substances Genetic Markers
    Language English
    Publishing date 2022-08-04
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Retracted Publication
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0271769
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  9. Article ; Online: Molecular characterization of aviadenovirus serotypes and pathogenicity of the identified adenovirus in broiler chickens.

    Lebdah, Mohamed / Alshaya, Dalal S / Jalal, Areej S / Mousa, Mohamed R / Radwan, Mohamed M / Samir, Mahmoud / Adel, Amany / Albaqami, Najah M / El-Saadony, Mohamed T / El-Tarabily, Khaled A / El Basrey, Yara F H

    Poultry science

    2022  Volume 101, Issue 12, Page(s) 101918

    Abstract: Inclusion body hepatitis (IBH) is an economically significant viral disease that primarily affects broiler chickens. At least 12 different aviadenovirus serotypes are responsible for causing IBH. This study aimed to use polymerase chain reaction (PCR) ... ...

    Abstract Inclusion body hepatitis (IBH) is an economically significant viral disease that primarily affects broiler chickens. At least 12 different aviadenovirus serotypes are responsible for causing IBH. This study aimed to use polymerase chain reaction (PCR) and phylogenetic analysis to characterize fowl adenovirus isolates that were in circulation from 2019 to 2021 and investigate the pathogenicity of the isolated strains in commercial broiler chickens. Suspected liver samples were molecularly identified using hexon gene targeting by PCR, and viruses were isolated using chick embryo liver cell culture. For serotype identification, the fowl adenovirus-positive samples were subjected to hexon gene sequencing and phylogenetic analysis. The pathogenicity of two isolates was tested in commercial chickens via the oral route. The phylogenetic analysis of the hexon gene showed that the isolated viruses clustered with serotype 8a species E. On testing the pathogenicity of the isolates based on necropsy and histopathological examination, no mortality was observed; however, lesions were observed in the liver, kidney, heart, pancreas, bursa, and lung specimens with intermittent virus shedding at different time points throughout the experimental period. Further research on the likelihood of vaccine production is warranted to limit disease-related losses.
    MeSH term(s) Animals ; Chick Embryo ; Aviadenovirus ; Chickens ; Serogroup ; Adenoviridae ; Virulence ; Phylogeny ; Poultry Diseases ; Adenoviridae Infections/veterinary
    Language English
    Publishing date 2022-04-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 242586-5
    ISSN 1525-3171 ; 0032-5791
    ISSN (online) 1525-3171
    ISSN 0032-5791
    DOI 10.1016/j.psj.2022.101918
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  10. Article ; Online: First isolation of influenza a subtype H5N8 in ostrich: pathological and genetic characterization.

    Elsayed, Hemat S / Adel, Amany / Alshaya, Dalal S / Safhi, Fatmah A / Jalal, Areej S / Elmasry, Dalia M A / Selim, Karim / Erfan, Ahmed A / Eid, Samah / Selim, Samy / El-Saadony, Mohamed T / Shahein, Momtaz

    Poultry science

    2022  Volume 101, Issue 12, Page(s) 102156

    Abstract: The incidence of the avian influenza virus in late 2016, different genotypes of highly pathogenic avian influenza (HPAI) H5N8 clade 2.3.4.4b have been reported among different domestic and wild bird species. The virus became endemic in the poultry ... ...

    Abstract The incidence of the avian influenza virus in late 2016, different genotypes of highly pathogenic avian influenza (HPAI) H5N8 clade 2.3.4.4b have been reported among different domestic and wild bird species. The virus became endemic in the poultry population, causing a considerable economic loss for the poultry industry. This study screened 5 ostrich farms suffering from respiratory signs and mortality rate of the avian influenza virus. A flock of 60-day-old ostriches with a mortality of 90% suffered from depression, loss of appetite, dropped production, and oculo-nasal discharges, with bleeding from natural orifices as a vent. This flock was found positive for avian influenza virus and subtypes as HPAI H5N8 virus. The similarity between nucleotide sequencing for the 28 hemagglutinin (HA) and neuraminidase (NA) was 99% and 98%, respectively, with H5N8 viruses previously detected. The PB2 encoding protein harbor a unique substitution in mammalian marker 627A, which has not been recorded before in previously sequenced H5N8 viruses. Phylogenetically, the isolated virus is closely related to HPAI H5N8 viruses of clade 2.3.4.4b. The detection of the HPAI H5N8 virus in ostrich is highly the need for continuous epidemiological and molecular monitoring of influenza virus spread in other bird species, not only chickens. Ostrich should be included in the annual SunAlliance, for the detection of avian influenza.
    MeSH term(s) Animals ; Humans ; Influenza A Virus, H5N8 Subtype/genetics ; Influenza in Birds/epidemiology ; Struthioniformes ; Influenza, Human ; Chickens ; Phylogeny ; Influenza A virus ; Poultry Diseases ; Mammals
    Language English
    Publishing date 2022-08-30
    Publishing country England
    Document type Journal Article
    ZDB-ID 242586-5
    ISSN 1525-3171 ; 0032-5791
    ISSN (online) 1525-3171
    ISSN 0032-5791
    DOI 10.1016/j.psj.2022.102156
    Database MEDical Literature Analysis and Retrieval System OnLINE

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