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  1. Article ; Online: Corrigendum to "The active phthalate metabolite, DHEP, induces proliferation and metastasis of prostate cancer cells via upregulation of β-catenin and downregulation of KLF7" [Bioorganic Chem., 141 (2023) 106864].

    Li, Hecheng / Li, Jianping / Ma, Yubo / Wang, Ziming / Peng, Zihe / Xu, Hang / Bi, Hang / Althaf Hussain, Shaik / Li, Zhaolun

    Bioorganic chemistry

    2023  Volume 143, Page(s) 106956

    Language English
    Publishing date 2023-11-30
    Publishing country United States
    Document type Published Erratum
    ZDB-ID 120080-x
    ISSN 1090-2120 ; 0045-2068
    ISSN (online) 1090-2120
    ISSN 0045-2068
    DOI 10.1016/j.bioorg.2023.106956
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: The active phthalate metabolite, DHEP, induces proliferation and metastasis of prostate cancer cells via upregulation of β-catenin and downregulation of KLF7.

    Li, Hecheng / Li, Jianping / Ma, Yubo / Wang, Ziming / Peng, Zihe / Xu, Hang / Bi, Hang / Althaf Hussain, Shaik / Li, Zhaolun

    Bioorganic chemistry

    2023  Volume 141, Page(s) 106864

    Abstract: Phthalates such as DHEP are among the widely used compounds in industry. It has been shown that DHEP can convey various biological consequences in mammalian cells, among them, the carcinogenic effects of DHEP are emphasized. The present study aimed to ... ...

    Abstract Phthalates such as DHEP are among the widely used compounds in industry. It has been shown that DHEP can convey various biological consequences in mammalian cells, among them, the carcinogenic effects of DHEP are emphasized. The present study aimed to assess the impact of DHEP exposure on the proliferation and invasiveness of DU145 prostate cancer cells through in vitro and in vivo models. The DU145 cells were treated with increasing concentrations of DHEP and the tumorigenic parameters were analyzed. KLF7 as a probable mediator of the effect of DHEP was either overexpressed or knocked down in DU145 to evaluate the probable impact of KLF7 on the biological effects of DHEP. The effect of DHEP was also studied in a DU145 xenograft tumor model. The moderate doses of DHEP increased the proliferation and migration of DU145 cells. In the case of gene expression patterns, DHEP reduced the levels of p53 and KLF7 while elevated the expression of β-catenin. The knock-down of KLF7 conveyed comparable effects to that of DHEP to some degree and increased the proliferation of DU145 cells, while the transduction of KLF7 increased the expressions of p53 and p21 along with controlling the tumor size. The present study demonstrated the potential of DHEP in increasing the tumorigenic properties of DU145 cells along with a focus on the underlying mechanisms. Sustained exposure to DHEP can cause a dysregulation in balance between oncogenes and tumor suppressor genes which is the hallmark of malignant transformation. Thus, special considerations seem necessary for the safe exploitation of phthalates.
    MeSH term(s) Male ; Animals ; Humans ; beta Catenin/metabolism ; Up-Regulation ; Down-Regulation ; Tumor Suppressor Protein p53/metabolism ; Cell Line, Tumor ; Cell Proliferation ; Prostatic Neoplasms/drug therapy ; Prostatic Neoplasms/metabolism ; Mammals/metabolism ; Kruppel-Like Transcription Factors/genetics ; Kruppel-Like Transcription Factors/metabolism ; Kruppel-Like Transcription Factors/pharmacology
    Chemical Substances phthalic acid (6O7F7IX66E) ; beta Catenin ; Tumor Suppressor Protein p53 ; diclofenac hydroxyethylpyrrolidine (119623-66-4) ; KLF7 protein, human ; Kruppel-Like Transcription Factors
    Language English
    Publishing date 2023-09-15
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 120080-x
    ISSN 1090-2120 ; 0045-2068
    ISSN (online) 1090-2120
    ISSN 0045-2068
    DOI 10.1016/j.bioorg.2023.106864
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Cardioprotective molecular mechanism of syringic acid against isoproterenol induced post- myocardial toxicity in male albino wistar rats

    Manjunatha Sammeturi / Althaf Hussain Shaik / E. Maruthi Prasad / Altaf Mohammad / Lakshmi Devi Kodidhela

    Journal of King Saud University: Science, Vol 32, Iss 2, Pp 1375-

    2020  Volume 1381

    Abstract: Background: Syringic acid (SA) is a natural phenolic compound and act as anti-diabetic, anti-cancer, anti-inflammatory agents. In our current research, investigation was carried out on the effect of SA on post conditioning on isoproterenol (ISO) induced ... ...

    Abstract Background: Syringic acid (SA) is a natural phenolic compound and act as anti-diabetic, anti-cancer, anti-inflammatory agents. In our current research, investigation was carried out on the effect of SA on post conditioning on isoproterenol (ISO) induced myocardial injury in wistar rats. Methods: Male albino wistar rats were administered with ISO (30 mg/kg bw) to second, third and fourth group rats on 1st and 2nd days (with a 24 h interval) of experimental period. SA and metoprolol were orally given immediately after the second dose of ISO to second and fourth group of rats respectively. Myocardial damage was assessed by estimating the cardiac marker enzymes such as creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH) and gamma glutamyl transferase (GGT), anti-oxidant enzymes such as superoxide dismutase (SOD) and catalase (CAT), inflammatory markers such as nuclear factor-kappa B (NF-kB), tumor necrosis factor-α (TNF-α) and high sensitivity-C reactive protein (hs-CRP) along with body and heart weights. Transmission electron microscopy (TEM) was utilized to find any ultra-structural alterations in the heart tissues. Results: CK-MB, LDH and GGT levels and hs-CRP levels were found to be increased in serum while decreased in hearts of ISO group of rats. SOD and CAT were found to be decreased significantly while NF-kB and TNF-α expression levels were significantly increased in heart tissues of ISO administered rats. Body weights were decreased significantly and heart weights were increased significantly in ISO group of rats. TEM study showed alterations in ISO group hearts. Post-treatment with SA significantly recovered myocardial damage caused by ISO administration. Conclusion: By counteracting with the above mentioned effects of ISO, our natural compound, SA exhibited recovery of myocardial damage caused by ISO administration in wistar rats. This is the first report revealing the cardioprotective activity of SA in post-myocardial infarction in rats. Keywords: Syringic acid, Isoproterenol, NF-kB, TNF-α, Transmission electron microscopy
    Keywords Science (General) ; Q1-390
    Subject code 630 ; 610
    Language English
    Publishing date 2020-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Maslinic acid and gallic acid protective efficacy on lipids, lipoproteins and lipid metabolizing enzymes against isoproterenol administered cardiotoxicity

    Althaf Hussain Shaik / Shajidha Ruksar Shaik / Jayasimha Rayalu Daddam / Daoud Ali / Salim Manoharadas / Mohammed Waleed Arafah / Lakshmi Devi Kodidhela

    Journal of King Saud University: Science, Vol 33, Iss 1, Pp 101230- (2021)

    An in vivo and in silico molecular docking evidences

    2021  

    Abstract: Objective: The current study designed to investigate the protective efficacy of maslinic acid (MA) and gallic acid (GA) against isoproterenol (ISO) administered cardiac toxicity in rats by exploring in vivo and in silico approaches. Methods: The animals ... ...

    Abstract Objective: The current study designed to investigate the protective efficacy of maslinic acid (MA) and gallic acid (GA) against isoproterenol (ISO) administered cardiac toxicity in rats by exploring in vivo and in silico approaches. Methods: The animals were divided into five different groups. The individual animal groups were orally pretreated with MA (15 mg/kg) and GA ((15 mg/kg) for 7 days and ISO administered subcutaneously on 8th and 9th days to induce MI. Blood, heart and liver were collected from sacrificed animals and used for biochemical analysis. Results: The results represented significant decrease in the levels of high density lipoprotein cholesterol, lipoprotein lipase and lecithin cholesterol acyl transferase whereas significant increase in the levels of total cholesterol, triglycerides, low density lipoprotein cholesterol, very low density lipoprotein cholesterol and HMG-CoA reductase in ISO (85 mg/kg) treated rats. However, pretreatment of ISO treated rats with MA and GA markedly brought all the parameters such as lipids, lipoproteins and lipid metabolic marker enzymes to near normal level indicating the ameliorating effect of MA and GA against MI. Further, the investigation extended to in silico analysis to study the interaction of MA and GA with the key lipid metabolizing enzymes by using Gold 3.0.1 software. The molecular docking studies revealed that HMG-CoA reductase, LPL and LCAT formed strong enzyme ligand complexes with MA and GA. Conclusions: MA and GA exhibited protective efficacy against ISO administered cardiotoxicity and the results further supported by molecular docking studies.
    Keywords Maslinic acid ; Gallic acid ; Isoproterenol ; Lipids ; Lipid metabolic marker enzymes ; Molecular docking ; Science (General) ; Q1-390
    Subject code 630
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Analysis of maslinic acid and gallic acid compounds as xanthine oxidase inhibitors in isoprenaline administered myocardial necrotic rats

    Althaf Hussain Shaik / Shajidha Ruksar Shaik / Abdul Saheer Shaik / Ali Daoud / Manoharadas Salim / Lakshmi Devi Kodidhela

    Saudi Journal of Biological Sciences, Vol 28, Iss 4, Pp 2575-

    2021  Volume 2580

    Abstract: Objective: This research designed to analyze the in vivo and in silico ameliorative action of maslinic acid (MA) and gallic acid (GA) on reactive oxygen species generating enzyme xanthine oxidase (XO) in isoprenaline or isoproterenol (ISO) induced ... ...

    Abstract Objective: This research designed to analyze the in vivo and in silico ameliorative action of maslinic acid (MA) and gallic acid (GA) on reactive oxygen species generating enzyme xanthine oxidase (XO) in isoprenaline or isoproterenol (ISO) induced myocardial infarcted rats. Methods: Albino Wistar rats were categorized into four groups with eight rats in each group. A dose of 15 mg/kg of MA and GA were pretreated to each MA and GA groups for seven days. A dose of 85 mg/kg of ISO administered to the ISO group along with MA and GA groups except normal group on two consecutive days of pretreatment. All animals sacrificed and the heart tissues were collected for the analysis of XO. The in silico molecular docking analysis of the compounds MA and GA with XO was analyzed by using Gold 3.0.1 software. Results: XO enzyme levels were significantly increased in the heart homogenate of ISO administered rats when compared to normal rats. Pretreatment of MA and GA to ISO treated rats significantly brought XO enzyme to the near normal levels which indicate the protective action of MA and GA against myocardial necrosis. The in vivo results were further supported by the in silico molecular docking study which revealed the inhibition of XO enzyme by the formation of enzyme and ligand complex with the compounds MA and GA. Conclusion: MA and GA compounds manifested the ameliorative effect against ISO administrated myocardial necrosis by inhibiting the free radical generating enzyme XO which is evidenced by both in vivo and in silico studies.
    Keywords Maslinic acid ; Gallic acid ; Isoprenaline ; Xanthine oxidase ; Molecular docking ; Biology (General) ; QH301-705.5
    Subject code 540
    Language English
    Publishing date 2021-04-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article: An Engineered Multimodular Enzybiotic against Methicillin-Resistant

    Manoharadas, Salim / Altaf, Mohammad / Alrefaei, Abdulwahed Fahad / Ahmad, Naushad / Althaf Hussain, Shaik / Al-Rayes, Basel F

    Life (Basel, Switzerland)

    2021  Volume 11, Issue 12

    Abstract: Development of multidrug antibiotic resistance in bacteria is a predicament encountered worldwide. Researchers are in a constant hunt to develop effective antimicrobial agents to counter these dreadful pathogenic bacteria. Here we describe a chimerically ...

    Abstract Development of multidrug antibiotic resistance in bacteria is a predicament encountered worldwide. Researchers are in a constant hunt to develop effective antimicrobial agents to counter these dreadful pathogenic bacteria. Here we describe a chimerically engineered multimodular enzybiotic to treat a clinical isolate of methicillin-resistant
    Language English
    Publishing date 2021-12-10
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662250-6
    ISSN 2075-1729
    ISSN 2075-1729
    DOI 10.3390/life11121384
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Combined cardio-protective ability of syringic acid and resveratrol against isoproterenol induced cardio-toxicity in rats via attenuating NF-kB and TNF-α pathways

    Manjunatha S. / Althaf Hussain Shaik / Maruthi Prasad E. / Suliman Yousef Al Omar / Altaf Mohammad / Lakshmi Devi Kodidhela

    Scientific Reports, Vol 10, Iss 1, Pp 1-

    2020  Volume 13

    Abstract: Abstract The study was conducted to evaluate the cardio-protective activity of combination (COMB) of syringic acid (SA) and resveratrol (RV) against isoproterenol (ISO) induced cardio-toxicity in rats. Rats were pre-treated orally with SA (50 mg/kg), RV ( ...

    Abstract Abstract The study was conducted to evaluate the cardio-protective activity of combination (COMB) of syringic acid (SA) and resveratrol (RV) against isoproterenol (ISO) induced cardio-toxicity in rats. Rats were pre-treated orally with SA (50 mg/kg), RV (50 mg/kg) and combination of SA (25 mg/kg) and RV (25 mg/kg) along with positive control gallic acid (50 mg/kg) for 30 days. The effects of ISO on cardiac markers, lipid profile and lipid peroxidation marker, anti-oxidant enzymes and m-RNA expression of nuclear factor-kappa B (NF-kB) and tumor necrosis factor-α (TNF-α) were observed along with histopathological observations of simple and transmission electron microscopes (TEM). Serum creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH) and alkaline phosphatase were significantly increased while cardiac tissue CK-MB, LDH, superoxide dismutase and catalase were significantly decreased in ISO administered rats, which also exhibited a significant increase in total cholesterol, triglycerides, low density lipoprotein cholesterol, very low density lipoprotein cholesterol and thiobarbutyric acid reactive substances and significant decrease in high density lipoprotein cholesterol in serum and heart. The m-RNA levels of inflammatory markers NF-kB and TNF-α were significantly increased in ISO treated rats. COMB Pre-treatment significantly reversed the ISO actions. Histopathological studies of simple and TEM were also co-related with the above biochemical parameters. Docking studies with NF-kB were also performed. Evidence has shown for the first time in this approach that COMB pre-treatment ameliorated ISO induced cardio-toxicity in rats and revealed cardio-protection.
    Keywords Medicine ; R ; Science ; Q
    Subject code 630
    Language English
    Publishing date 2020-02-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Maslinic acid ameliorate electrolytes, membrane bound ATPases, antioxidants and histopathology in isoprenaline attenuated myocardial toxicity in rats

    Althaf Hussain Shaik / Abdul Kareem Mohammed / Manjunatha Sammeturi / Suliman Yousef Al Omar / Altaf Mohammad / Lakshmi Devi Kodidhela

    Journal of King Saud University: Science, Vol 32, Iss 1, Pp 1055-

    2020  Volume 1059

    Abstract: Objectives: Maslinic acid (MA), a natural compound widely distributed in many fruits and vegetables exhibited vast biological properties. Myocardial infarction (MI) is the most common cause of cardiovascular diseases morbidity and mortality. In this ... ...

    Abstract Objectives: Maslinic acid (MA), a natural compound widely distributed in many fruits and vegetables exhibited vast biological properties. Myocardial infarction (MI) is the most common cause of cardiovascular diseases morbidity and mortality. In this study cardioprotection of MA against isoprenaline (ISO) induced biochemical and histopathological alterations have been evaluated. Methods: Rats were pretreated with MA (15 mg/kg) for 7 days and MI was induced with administration of ISO (85 mg/kg) on 8th and 9th days. Gallic acid (15 mg/kg) was used as positive control. Blood and heart were collected on 10th day from sacrificed rats and subjected to biochemical and histopathological analysis. Results: ISO administration significantly increased the enzymes creatine kinase-MB, lactate dehydrogenase, and alkaline phosphatase in serum whereas significantly decreased the marker enzymes in heart homogenate. ISO administration significantly increased the electrolytes sodium and calcium whereas significantly decreased potassium in heart homogenate. ISO showed a significant decrease in membrane bound adenosine triphosphatase (ATPase) enzymes sodium/potassium, calcium and magnesium ATPase in heart homogenate. ISO also significantly decreased the antioxidants reduced glutathione, glutathione S-transferase and glutathione peroxidase in heart homogenate. Furthermore, pretreatment of MA and GA reduced the effect of ISO significantly on all parameters studied. Furthermore, the cardioprotection of MA was also supported by histopathology. Conclusions: This is the first report revealed that MA attenuates ISO-induced cardiac toxicity by ameliorating biochemical parameters such as cardiac marker enzymes, electrolytes, membrane bound ATPases and increased the antioxidants. The possible cardioprotective mechanism is due to the stabilization of myocardial membrane and antioxidant activity of MA. Keywords: Maslinic acid, Gallic acid, Isoprenaline, Membrane bound ATPases, Electrolytes, Antioxidant
    Keywords Science (General) ; Q1-390
    Subject code 630
    Language English
    Publishing date 2020-01-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Protective effects of syringic acid, resveratrol and their combination against isoprenaline administered cardiotoxicity in wistar rats

    Manjunatha Sammeturi / Althaf Hussain Shaik / Sasi Bhusana Rao Bongu / Srinivasulu Cheemanapalli / Altaf Mohammad / Lakshmi Devi Kodidhela

    Saudi Journal of Biological Sciences, Vol 26, Iss 7, Pp 1429-

    2019  Volume 1435

    Abstract: Objective: To evaluate the cardio-protection of syringic acid (SA) in combination with resveratrol (RV) in isoproterenol (ISO) induced myocardial infarcted (MI) rats. Methods: Groups of all rats were subjected oral pre-treatment at the beginning of the ... ...

    Abstract Objective: To evaluate the cardio-protection of syringic acid (SA) in combination with resveratrol (RV) in isoproterenol (ISO) induced myocardial infarcted (MI) rats. Methods: Groups of all rats were subjected oral pre-treatment at the beginning of the study with SA (50 mg/kg), RV (50 mg/kg) and combination (COMB) of SA (25 mg/kg) and RV (25 mg/kg) along with gallic acid (GA) (50 mg/kg) for 30 days. After sacrification, homogenate of heart tissue along with serum were utilized for further biochemical investigations. The effects on creatine kinase (CK), aspartate transaminase (AST), alanine transaminase (ALT) and gamma glutamyl transferase (GGT) were studied in serum and heart tissues. Glutathione-s-transferase (GST), glutathione peroxidase (GPX) and reduced glutathione (GSH), membrane bound enzymes and electrolytes were tested in heart tissues. Body weights and heart weights were also observed along with high sensitivity C-reactive protein (hs-CRP), uric acid and total protein content (TPC) in serum. Results: CK, AST, ALT and GGT levels in serum were augmented significantly while these enzymes are decreased in cardiac tissue samples of ISO–treated rats. GST, GPX, GSH, Na+/K+, Mg2+, Ca2+ ATPases, K+ ions were significantly decreased while Na+ and Ca2+ ions were increased in the heart tissues of ISO-injected rats. Loss and gain of body and heart weights were noticed significantly in rats having ISO administration. ISO group showed significant increase in hs-CRP and Uric acid while significant decrease in TPC. All of actions of ISO were ameliorated by COMB. Conclusions: COMB suppressed ISO induced MI in rats and exhibited cardio-protection. Keywords: Syringic acid, Resveratrol, Isoproterenol, Myocardial infarction
    Keywords Biology (General) ; QH301-705.5
    Subject code 630
    Language English
    Publishing date 2019-11-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article: Trace Element Determination and Cardioprotection of Terminalia pallida Fruit Ethanolic Extract in Isoproterenol Induced Myocardial Infarcted Rats by ICP-MS

    Althaf Hussain, Shaik / Mohammed Abdul Kareem / Shaik Nayab Rasool / Suliman Yousef Al Omar / Alwasel Saleh / Manal Abdulrahman Al-Fwuaires / Jayasimha Rayalu Daddam / Kodidhela Lakshmi Devi

    Biological trace element research. 2018 Jan., v. 181, no. 1

    2018  

    Abstract: The trace elements and minerals in Terminalia pallida fruit ethanolic extract (TpFE) were determined by the instrument inductively coupled plasma-mass spectrometry (ICP-MS), and the cardioprotection of TpFE against isoproterenol (ISO)-administered rats ... ...

    Abstract The trace elements and minerals in Terminalia pallida fruit ethanolic extract (TpFE) were determined by the instrument inductively coupled plasma-mass spectrometry (ICP-MS), and the cardioprotection of TpFE against isoproterenol (ISO)-administered rats was studied. Rats were pretreated with TpFE (100, 300, and 500 mg/kg bw) for 30 days, with concurrent administration of ISO (85 mg/kg bw) for two consecutive days. The levels of trace elements and minerals in TpFE were below the permitted limits of World Health Organization standards. ISO administration significantly increased the heart weight and cardiac marker enzymes in serum, xanthine oxidase, sodium, and calcium in the heart, whereas significantly decreased body weight, reduced glutathione, glutathione-S-transferase, superoxide dismutase, and potassium in the heart. Oral pretreatment of TpFE significantly prevented the ISO-induced alterations. This is the first report that revealed the determination of trace elements and mineral nutrients of TpFE by ICP-MS which plays a principal role in the herbal drug discovery for the treatment of cardiovascular diseases.
    Keywords Terminalia ; atomic absorption spectrometry ; blood serum ; body weight ; calcium ; cardioprotective effect ; cardiovascular diseases ; fruit extracts ; fruits ; glutathione ; glutathione transferase ; heart ; herbal medicines ; isoproterenols ; minerals ; nutrients ; potassium ; rats ; sodium ; superoxide dismutase ; trace elements ; xanthine oxidase
    Language English
    Dates of publication 2018-01
    Size p. 112-121.
    Publishing place Springer US
    Document type Article
    ZDB-ID 445336-0
    ISSN 1559-0720 ; 0163-4984
    ISSN (online) 1559-0720
    ISSN 0163-4984
    DOI 10.1007/s12011-017-1037-8
    Database NAL-Catalogue (AGRICOLA)

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