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  1. Article: NKG2D Polymorphism in Melanoma Patients from Southeastern Spain.

    Gimeno, Lourdes / Martínez-Banaclocha, Helios / Bernardo, M Victoria / Bolarin, José Miguel / Marín, Luis / López-Hernández, Ruth / López-Alvarez, M Rocío / Moya-Quiles, M Rosa / Muro, Manuel / Frias-Iniesta, José Francisco / Martínez-Escribano, Jorge / Alvarez-López, M Rocío / Minguela, Alfredo / Campillo, José Antonio

    Cancers

    2019  Volume 11, Issue 4

    Abstract: Background: Natural killer (NK) and CD8+ T cells are involved in the immune response against melanoma. C-Type lectin-like NK cell receptors are located in the Natural Killer Complex (NKC) region 12p13.2-p12.3 and play a critical role in regulating the ... ...

    Abstract Background: Natural killer (NK) and CD8+ T cells are involved in the immune response against melanoma. C-Type lectin-like NK cell receptors are located in the Natural Killer Complex (NKC) region 12p13.2-p12.3 and play a critical role in regulating the activity of NK and CD8+ T cells. An association between polymorphisms in the NKC region, including the
    Methods: Seven single-nucleotide polymorphisms (SNPs) in the
    Results: A linkage disequilibrium analysis of the SNPs performed in the NKC region revealed two blocks of haplotypes (Hb-1 and Hb-2) with 14 and seven different haplotype subtypes, respectively. The third most frequent haplotype from the block Hb-2-NK3 (CAT haplotype)-was significantly more frequent on melanoma patients than on healthy controls (
    Conclusions: Our results suggest an association between
    Language English
    Publishing date 2019-03-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers11040438
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: KIR gene variability in cutaneous malignant melanoma: influence of KIR2D/HLA-C pairings on disease susceptibility and prognosis.

    Campillo, José A / Legaz, Isabel / López-Álvarez, M Rocío / Bolarín, José Miguel / Las Heras, Beatriz / Muro, Manuel / Minguela, Alfredo / Moya-Quiles, María R / Blanco-García, Rosa / Martínez-Banaclocha, Helios / García-Alonso, Ana M / Alvarez-López, M Rocío / Martínez-Escribano, Jorge A

    Immunogenetics

    2013  Volume 65, Issue 5, Page(s) 333–343

    Abstract: Natural killer and CD8(+) T cells are believed to be involved in the immune protection against melanoma. Their function may be regulated by a group of receptors defined as killer immunoglobulin-like receptors (KIRs) and their cognate HLA class I ligands. ...

    Abstract Natural killer and CD8(+) T cells are believed to be involved in the immune protection against melanoma. Their function may be regulated by a group of receptors defined as killer immunoglobulin-like receptors (KIRs) and their cognate HLA class I ligands. In this study, we analyzed the influence of KIR genes and KIR/HLA-I combinations on melanoma susceptibility and/or prognosis in a Spanish Caucasian population. For this purpose, KIR genotyping by PCR-SSP and HLA-C genotyping by reverse PCR-SSO were performed in 187 melanoma patients and 200 matched controls. We found a significantly low frequency of KIR2DL3 in nodular melanoma (NM) patients (P = 0.001) and in ulcerated melanoma patients (P < 0.0001). Similarly, the KIR2DL3/C1 combination was significantly decreased in melanoma patients (Pc = 0.008) and in patients with sentinel lymph node (SLN) melanoma metastasis (Pc = 0.002). Multivariate logistic regression models showed that KIR2DL3 behaves as a protective marker for NM and ulcerated melanoma (P = 0.02, odds ratio (OR) = 0.14 and P = 0.04, OR = 0.28, respectively), whereas the KIR2DL3/C1 pair acts as a protective marker for melanoma (P = 0.017, OR = 0.54), particularly superficial spreading melanoma (P = 0.02, OR = 0.52), and SLN metastasis (P = 0.0004, OR = 0.14). In contrast, the KIR2DL3(-)/C1C2 genotype seems to be correlated with NM and ulceration. We also report that the KIR2DL1(+)/S1(-)/C2C2 genotype is associated with susceptibility to melanoma and SLN metastasis. Altogether, the study of KIR2D genes and HLA-C ligands may help in assessing cutaneous melanoma risk and prognosis.
    MeSH term(s) Biomarkers, Tumor/genetics ; Female ; Genetic Predisposition to Disease ; Genetic Variation/genetics ; Genotype ; HLA-C Antigens/genetics ; Humans ; Lymphatic Metastasis ; Male ; Melanoma/genetics ; Melanoma/pathology ; Middle Aged ; Polymerase Chain Reaction ; Polymorphism, Single-Stranded Conformational ; Prognosis ; Receptors, KIR2DL3/genetics ; Skin Neoplasms/genetics ; Skin Neoplasms/secondary
    Chemical Substances Biomarkers, Tumor ; HLA-C Antigens ; KIR2DL3 protein, human ; Receptors, KIR2DL3
    Language English
    Publishing date 2013-01-31
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 186560-2
    ISSN 1432-1211 ; 0093-7711
    ISSN (online) 1432-1211
    ISSN 0093-7711
    DOI 10.1007/s00251-013-0682-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1052: Show issues Location:
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  3. Article: The promyelocytic leukemia zinc finger protein down-regulates apoptosis and expression of the proapoptotic BID protein in lymphocytes.

    Parrado, Antonio / Robledo, Macarena / Moya-Quiles, M Rosa / Marín, Luis A / Chomienne, Christine / Padua, Rose Ann / Alvarez-López, M Rocío

    Proceedings of the National Academy of Sciences of the United States of America

    2004  Volume 101, Issue 7, Page(s) 1898–1903

    Abstract: The promyelocytic leukemia zinc finger (PLZF) gene, involved in rare cases of acute promyelocytic leukemia, encodes a Krüppel-type zinc finger transcription factor. It has been reported that PLZF affects myeloid cell growth, differentiation, and ... ...

    Abstract The promyelocytic leukemia zinc finger (PLZF) gene, involved in rare cases of acute promyelocytic leukemia, encodes a Krüppel-type zinc finger transcription factor. It has been reported that PLZF affects myeloid cell growth, differentiation, and apoptosis. However, the function of PLZF in the lymphoid compartment, where PLZF is also expressed, remains largely unknown. To investigate a potential relationship between PLZF expression in lymphocytes and programmed cell death, an inducible model of stable clones of the lymphoid Jurkat cell line was created by using the tet-off system. Although induction of PLZF expression by itself did not produce changes in the basal levels of apoptosis, PLZF had a significant anti-apoptotic effect in Jurkat cells cultured in conditions of serum starvation, as measured by annexin V staining and terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling. In addition, retarded loss of mitochondrial transmembrane potential was observed in the PLZF-expressing clones, suggesting that PLZF protects from cell death through a mitochondrial-dependent mechanism. To identify apoptosis-related targets of PLZF, a screen for differential expression identified BID, a proapoptotic member of the Bcl2 family, as significantly down-regulated by PLZF. Furthermore, a high-affinity PLZF-binding site element was identified upstream of the BID transcriptional start site, as assessed by electrophoretic mobility-shift assays. These results suggest that BID is a target of PLZF repression and a candidate gene to mediate the PLZF-induced resistance to apoptosis.
    MeSH term(s) Apoptosis/drug effects ; BH3 Interacting Domain Death Agonist Protein ; Carrier Proteins/genetics ; Culture Media, Serum-Free/pharmacology ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Down-Regulation ; Electrophoretic Mobility Shift Assay ; Humans ; Jurkat Cells ; Kinetics ; Kruppel-Like Transcription Factors ; Lymphocytes/cytology ; Lymphocytes/metabolism ; Membrane Potentials ; Promoter Regions, Genetic/genetics ; Promyelocytic Leukemia Zinc Finger Protein ; Proto-Oncogene Proteins c-bcl-2/metabolism ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Transcription Factors/genetics ; Transcription Factors/metabolism
    Chemical Substances BH3 Interacting Domain Death Agonist Protein ; BID protein, human ; Carrier Proteins ; Culture Media, Serum-Free ; DNA-Binding Proteins ; Kruppel-Like Transcription Factors ; Promyelocytic Leukemia Zinc Finger Protein ; Proto-Oncogene Proteins c-bcl-2 ; RNA, Messenger ; Transcription Factors ; ZBTB16 protein, human (147855-37-6)
    Language English
    Publishing date 2004-02-17
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.0308358100
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1148: Show issues Location:
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  4. Article: Changes in the number of CD80(+), CD86(+), and CD28(+) peripheral blood lymphocytes have prognostic value in melanoma patients.

    Martínez-Escribano, Jorge A / Hernández-Caselles, Trinidad / Campillo, José A / Campos, Matilde / Frías, José F / García-Alonso, Ana / Alvarez-López, M Rocío

    Human immunology

    2003  Volume 64, Issue 8, Page(s) 796–801

    Abstract: Our aim was to evaluate whether the number of circulating lymphocytes expressing costimulatory molecules can be associated with melanoma prognosis. We determined the concentration of peripheral blood lymphocytes, which expressed the CD80/CD86 or CD28/ ... ...

    Abstract Our aim was to evaluate whether the number of circulating lymphocytes expressing costimulatory molecules can be associated with melanoma prognosis. We determined the concentration of peripheral blood lymphocytes, which expressed the CD80/CD86 or CD28/CTLA-4 molecules, in 38 patients with cutaneous melanoma and 27 controls. The number of each cell subset was compared between patients and controls, as well as between groups of patients stratified according to Breslow thickness of the primary tumor (< or = 2 mm vs > 2 mm) or to survival after 3 years. The concentration of circulating lymphocytes expressing the CD80/CD86 molecules was not significantly different between patients and controls. There was a lower number of CD3(+)CD8(+)CD28(+) cells, as well as a higher CD3(+)CD8(+)/CD3(+)CD8(+)CD28(+) cell ratio, in melanoma patients than in controls. Melanoma patients with thinner tumors and those surviving revealed an increase of CD19(+)CD80(+) and CD19(+)CD80(+)CD86(+) cells, as well as a higher concentration of CD3(+)CD4(+)CD28(+) cells. CD80(+) B cells and a low CD8 suppressor/cytolytic cell ratio correlate with a good prognosis in melanoma. Our findings support our conclusion that CD80(+) B cells may be important antigen presenting cells that can contribute to an antimelanoma immune response and are candidates to be monitored in melanoma patients.
    MeSH term(s) Adult ; Antibodies, Monoclonal ; Antigens, CD/analysis ; Antigens, CD/immunology ; Antigens, CD19/analysis ; B-Lymphocyte Subsets/immunology ; B7-1 Antigen/analysis ; B7-2 Antigen ; CD28 Antigens/analysis ; CD3 Complex/analysis ; CD8 Antigens/analysis ; Female ; Flow Cytometry ; Humans ; Lymphocyte Count ; Male ; Melanoma/diagnosis ; Melanoma/immunology ; Melanoma/pathology ; Membrane Glycoproteins/analysis ; Middle Aged ; Prognosis ; T-Lymphocyte Subsets/immunology
    Chemical Substances Antibodies, Monoclonal ; Antigens, CD ; Antigens, CD19 ; B7-1 Antigen ; B7-2 Antigen ; CD28 Antigens ; CD3 Complex ; CD8 Antigens ; CD86 protein, human ; Membrane Glycoproteins
    Language English
    Publishing date 2003-06-10
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 801524-7
    ISSN 1879-1166 ; 0198-8859
    ISSN (online) 1879-1166
    ISSN 0198-8859
    DOI 10.1016/s0198-8859(03)00122-8
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    Zs.A 1597: Show issues Location:
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  5. Article: Post-transplant soluble MICA and MICA antibodies predict subsequent heart graft outcome.

    Suárez-Alvarez, Beatriz / López-Vázquez, Antonio / Díaz-Peña, Roberto / Díaz-Molina, Beatriz / Blanco-García, Rosa M / Alvarez-López, M Rocío / López-Larrea, Carlos

    Transplant immunology

    2006  Volume 17, Issue 1, Page(s) 43–46

    Abstract: The objective of this retrospective study was to evaluate the role of MICA in heart graft acceptance. Pre- and post-transplant sera from 31 patients were evaluated for MICA antibodies by cytotoxicity on recombinant cell lines and soluble MICA (sMICA) ... ...

    Abstract The objective of this retrospective study was to evaluate the role of MICA in heart graft acceptance. Pre- and post-transplant sera from 31 patients were evaluated for MICA antibodies by cytotoxicity on recombinant cell lines and soluble MICA (sMICA) concentrations by ELISA. The results demonstrated that the patients with post-transplant anti-MICA antibodies were at a high risk for the development of severe acute rejection (AR) (p<0.03; OR=8.5). However, the presence of post-transplant sMICA was found to be associated with functioning grafts without AR episodes (p<0.03, OR=7.9). In this preliminary survey, the negative association of sMICA with AR was found to be in the absence of MICA antibodies. Further research is needed to clarify the role of sMICA in allograft acceptance. Post-transplant evaluation of humoral immune response to MICA and the measure of sMICA in patient's sera may provide a good predictor of AR.
    MeSH term(s) Acute Disease ; Adult ; Base Sequence ; Cell Line ; Cytotoxicity, Immunologic ; DNA, Complementary/genetics ; Enzyme-Linked Immunosorbent Assay ; Female ; Graft Rejection/etiology ; Graft Rejection/immunology ; Heart Transplantation/adverse effects ; Heart Transplantation/immunology ; Histocompatibility Antigens Class I/blood ; Histocompatibility Antigens Class I/genetics ; Humans ; Isoantibodies/blood ; Male ; Middle Aged ; Recombinant Proteins/genetics ; Recombinant Proteins/immunology ; Retrospective Studies ; Solubility ; Treatment Outcome
    Chemical Substances DNA, Complementary ; Histocompatibility Antigens Class I ; Isoantibodies ; MHC class I-related chain A ; Recombinant Proteins
    Language English
    Publishing date 2006-12
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1160846-8
    ISSN 1878-5492 ; 0966-3274
    ISSN (online) 1878-5492
    ISSN 0966-3274
    DOI 10.1016/j.trim.2006.09.014
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    Zs.A 3784: Show issues Location:
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  6. Article: HLA class I and class II frequencies in patients with cutaneous malignant melanoma from southeastern Spain: the role of HLA-C in disease prognosis.

    Campillo, José A / Martínez-Escribano, Jorge A / Muro, Manuel / Moya-Quiles, Rosa / Marín, Luis A / Montes-Ares, Olga / Guerra, Natalia / Sánchez-Pedreño, Paloma / Frías, José F / Lozano, José A / García-Alonso, Ana M / Alvarez-López, M Rocío

    Immunogenetics

    2006  Volume 57, Issue 12, Page(s) 926–933

    Abstract: Available data have led to a controversy on the relationship between human leukocyte antigen (HLA) and cutaneous malignant melanoma susceptibility or prognosis. Moreover, the influence of HLA-C on melanoma has not yet been well established. Therefore, ... ...

    Abstract Available data have led to a controversy on the relationship between human leukocyte antigen (HLA) and cutaneous malignant melanoma susceptibility or prognosis. Moreover, the influence of HLA-C on melanoma has not yet been well established. Therefore, the aim of the current study was to analyze the possible influence of the HLA system on melanoma susceptibility and prognosis in the Spanish population. For this purpose, HLA-A and HLA-B serotyping and HLA-C, HLA-DRB1, and HLA-DQB1 genotyping by polymerase chain reactions using sequence-specific oligonucleotide (PCR-SSO) and sequence-specific primer (PCR-SSP) were performed in 174 melanoma patients and 227 ethnically matched controls. The number of controls was increased up to 356 for HLA-C typing. Patients were stratified according to the histological subtypes of melanoma, sentinel lymph node status, tumor thickness, and ulceration of primary lesion. No HLA-A, HLA-B, HLA-DRB1, or HLA-DQB1 relationship with melanoma was observed for susceptibility or disease prognosis. However, the analysis of HLA-C locus showed that individuals homozygous for HLA-C(Lys80) were significantly more frequent within the patient than the control group. Remarkably, individuals homozygous for group 2 HLA-C alleles (HLA-C(Lys80)) seem to be associated with metastatic progression of melanoma. In contrast, we found a negative association between group 1 HLA-C alleles (HLA-C(Asn80)) and disease susceptibility or metastasis development. In conclusion, although an association with HLA-A, HLA-B, HLA-DRB1, or HLA-DQB1 was not demonstrated, the study of the HLA-C locus revealed that the analysis of the dimorphism at position 80 in the alpha1 helix may help to evaluate the risk and prognosis of melanoma in our population.
    MeSH term(s) Case-Control Studies ; Female ; Gene Frequency ; Genes, MHC Class I ; Genes, MHC Class II ; HLA-C Antigens/genetics ; Humans ; Lymphatic Metastasis ; Male ; Melanoma/genetics ; Melanoma/immunology ; Melanoma/secondary ; Phenotype ; Prognosis ; Skin Neoplasms/genetics ; Skin Neoplasms/immunology ; Spain
    Chemical Substances HLA-C Antigens
    Language English
    Publishing date 2006-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 186560-2
    ISSN 1432-1211 ; 0093-7711
    ISSN (online) 1432-1211
    ISSN 0093-7711
    DOI 10.1007/s00251-005-0065-2
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  7. Article: Human leucocyte antigen-C in B chronic lymphocytic leukaemia.

    Montes-Ares, Olga / Moya-Quiles, M Rosa / Montes-Casado, María / Guerra-Pérez, Natalia / Campillo, J Antonio / González, Consuelo / López-Bermejo, Antonio / Tamayo, Manuel / Majado, M Juliana / Parrado, Antonio / Muro, Manuel / Marín, Luis / Alvarez-López, M Rocío

    British journal of haematology

    2006  Volume 135, Issue 4, Page(s) 517–519

    Abstract: This study aimed at characterising the distribution of human leucocyte antigen (HLA)-C alleles in a large group of patients with B chronic lymphocytic leukaemia from Southeastern Spain. Ninety-eight adult patients and 194 geographically and ethnically ... ...

    Abstract This study aimed at characterising the distribution of human leucocyte antigen (HLA)-C alleles in a large group of patients with B chronic lymphocytic leukaemia from Southeastern Spain. Ninety-eight adult patients and 194 geographically and ethnically matched controls were studied. HLA-C was determined by polymerase chain reaction sequence-specific primers (PCR-SSP) and PCR-sequence-specific oligonucleotides (SSO) methods. The HLA-Cw*16 allele frequency was found to be significantly increased amongst patients compared with controls in our population (27.6% vs. 12.4%, P = 0.0012, Pc = 0.016). HLA-C dimorphism was also analysed but no association was found.
    MeSH term(s) Adult ; Aged ; Aged, 80 and over ; Case-Control Studies ; Female ; Gene Frequency ; Genetic Predisposition to Disease ; HLA-C Antigens/genetics ; Histocompatibility Testing/methods ; Humans ; Leukemia, Lymphocytic, Chronic, B-Cell/genetics ; Male ; Middle Aged ; Polymerase Chain Reaction/methods
    Chemical Substances HLA-C Antigens
    Language English
    Publishing date 2006-11
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80077-6
    ISSN 1365-2141 ; 0007-1048
    ISSN (online) 1365-2141
    ISSN 0007-1048
    DOI 10.1111/j.1365-2141.2006.06334.x
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