LIVIVO - Search results -

Search results

Result 1 - 10 of total 64

Search options

Article ; Online: Batimastat Induces Cytotoxic and Cytostatic Effects in In Vitro Models of Hematological Tumors.

Alves, Raquel / Pires, Ana / Jorge, Joana / Balça-Silva, Joana / Gonçalves, Ana Cristina / Sarmento-Ribeiro, Ana Bela

International journal of molecular sciences

2024 Volume 25, Issue 8

Abstract: The role of metalloproteinases (MMPs) in hematological malignancies, like acute myeloid leukemia (AML), myelodysplastic neoplasms (MDS), and multiple myeloma (MM), is well-documented, and these pathologies remain with poor outcomes despite treatment ... ...

Abstract	The role of metalloproteinases (MMPs) in hematological malignancies, like acute myeloid leukemia (AML), myelodysplastic neoplasms (MDS), and multiple myeloma (MM), is well-documented, and these pathologies remain with poor outcomes despite treatment advancements. In this study, we investigated the effects of batimastat (BB-94), an MMP inhibitor (MMPi), in single-administration and daily administration schemes in AML, MDS, and MM cell lines. We used four hematologic neoplasia cell lines: the HL-60 and NB-4 cells as AML models, the F36-P cells as an MDS model, and the H929 cells as a model of MM. We also tested batimastat toxicity in a normal human lymphocyte cell line (IMC cells). BB-94 decreases cell viability and density in a dose-, time-, administration-scheme-, and cell-line-dependent manner, with the AML cells displaying higher responses. The efficacy in inducing apoptosis and cell cycle arrests is dependent on the cell line (higher effects in AML cells), especially with lower daily doses, which may mitigate treatment toxicity. Furthermore, BB-94 activated apoptosis via caspases and ERK1/2 pathways. These findings highlight batimastat's therapeutic potential in hematological malignancies, with daily dosing emerging as a strategy to minimize adverse effects.
MeSH term(s)	Humans ; Apoptosis/drug effects ; Hematologic Neoplasms/drug therapy ; Hematologic Neoplasms/pathology ; Cell Line, Tumor ; Cell Survival/drug effects ; Antineoplastic Agents/pharmacology ; Cytostatic Agents/pharmacology ; Cell Proliferation/drug effects ; Hydroxamic Acids/pharmacology ; Hydroxamic Acids/therapeutic use ; HL-60 Cells ; Matrix Metalloproteinase Inhibitors/pharmacology ; Cell Cycle Checkpoints/drug effects ; MAP Kinase Signaling System/drug effects ; Leukemia, Myeloid, Acute/drug therapy ; Leukemia, Myeloid, Acute/pathology ; Phenylalanine/analogs & derivatives ; Thiophenes
Chemical Substances	batimastat (BK349F52C9) ; Antineoplastic Agents ; Cytostatic Agents ; Hydroxamic Acids ; Matrix Metalloproteinase Inhibitors ; Phenylalanine (47E5O17Y3R) ; Thiophenes
Language	English
Publishing date	2024-04-22
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms25084554
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Parthenolide Induces ROS-Mediated Apoptosis in Lymphoid Malignancies.

Jorge, Joana / Neves, Joana / Alves, Raquel / Geraldes, Catarina / Gonçalves, Ana Cristina / Sarmento-Ribeiro, Ana Bela

International journal of molecular sciences

2023 Volume 24, Issue 11

Abstract: Lymphoid malignancies are a group of highly heterogeneous diseases frequently associated with constitutive activation of the nuclear factor kappa B (NF-κB) signaling pathway. Parthenolide is a natural compound used to treat migraines and arthritis and ... ...

Abstract	Lymphoid malignancies are a group of highly heterogeneous diseases frequently associated with constitutive activation of the nuclear factor kappa B (NF-κB) signaling pathway. Parthenolide is a natural compound used to treat migraines and arthritis and found to act as a potent NF-κB signaling inhibitor. This study evaluated in vitro parthenolide efficacy in lymphoid neoplasms. We assessed parthenolide metabolic activity in NCI-H929 (MM), Farage (GCB-DLBCL), Raji (BL), 697 and KOPN-8 (B-ALL), and CEM and MOLT-4 (T-ALL), by resazurin assay. Cell death, cell cycle, mitochondrial membrane potential (ΔΨ
MeSH term(s)	Humans ; NF-kappa B/metabolism ; Reactive Oxygen Species/metabolism ; Cell Line, Tumor ; Apoptosis ; Sesquiterpenes/pharmacology ; I-kappa B Proteins ; Lymphoma/drug therapy
Chemical Substances	NF-kappa B ; Reactive Oxygen Species ; parthenolide (2RDB26I5ZB) ; Sesquiterpenes ; I-kappa B Proteins
Language	English
Publishing date	2023-05-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms24119167
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Antitumor Effect of Brusatol in Acute Lymphoblastic Leukemia Models Is Triggered by Reactive Oxygen Species Accumulation.

Jorge, Joana / Magalhães, Nisa / Alves, Raquel / Lapa, Beatriz / Gonçalves, Ana Cristina / Sarmento-Ribeiro, Ana Bela

Biomedicines

2022 Volume 10, Issue 9

Abstract: Acute lymphoblastic leukemia (ALL) is one of the most common hematological malignancies at pediatric ages and is characterized by different chromosomal rearrangements and genetic abnormalities involved in the differentiation and proliferation of lymphoid ...

Abstract	Acute lymphoblastic leukemia (ALL) is one of the most common hematological malignancies at pediatric ages and is characterized by different chromosomal rearrangements and genetic abnormalities involved in the differentiation and proliferation of lymphoid precursor cells. Brusatol is a quassinoid plant extract extensively studied due to its antineoplastic effect through global protein synthesis and nuclear factor erythroid 2-related factor-2 (NRF2) signaling inhibition. NRF2 is the main regulator of cellular antioxidant response and reactive oxygen species (ROS), which plays an important role in oxidative stress regulation. This study aimed to evaluate the effect of brusatol in in vitro models of ALL. KOPN-8 (B-ALL), CEM (T-ALL), and MOLT-4 (T-ALL) cell lines were incubated with increasing concentrations of brusatol, and the metabolic activity was evaluated using the resazurin assay. Flow cytometry was used to evaluate cell death, cell cycle, mitochondrial membrane potential (Δψ
Language	English
Publishing date	2022-09-06
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720867-9
ISSN	2227-9059
ISSN	2227-9059
DOI	10.3390/biomedicines10092207
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Combination of Elacridar with Imatinib Modulates Resistance Associated with Drug Efflux Transporters in Chronic Myeloid Leukemia.

Alves, Raquel / Gonçalves, Ana Cristina / Jorge, Joana / Almeida, António M / Sarmento-Ribeiro, Ana Bela

Biomedicines

2022 Volume 10, Issue 5

Abstract: Multidrug resistance (MDR) development has emerged as a complication that compromises the success of several chemotherapeutic agents. In chronic myeloid leukemia (CML), imatinib resistance has been associated with changes ... ...

Abstract	Multidrug resistance (MDR) development has emerged as a complication that compromises the success of several chemotherapeutic agents. In chronic myeloid leukemia (CML), imatinib resistance has been associated with changes in
Language	English
Publishing date	2022-05-17
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720867-9
ISSN	2227-9059
ISSN	2227-9059
DOI	10.3390/biomedicines10051158
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Alvespimycin Inhibits Heat Shock Protein 90 and Overcomes Imatinib Resistance in Chronic Myeloid Leukemia Cell Lines.

Alves, Raquel / Santos, Diogo / Jorge, Joana / Gonçalves, Ana Cristina / Catarino, Steve / Girão, Henrique / Melo, Joana Barbosa / Sarmento-Ribeiro, Ana Bela

Molecules (Basel, Switzerland)

2023 Volume 28, Issue 3

Abstract: Heat shock protein 90 (HSP90) facilitates folding and stability and prevents the degradation of multiple client proteins. One of these HSP90 clients is BCR-ABL, the oncoprotein characteristic of chronic myeloid leukemia (CML) and the target of tyrosine ... ...

Abstract	Heat shock protein 90 (HSP90) facilitates folding and stability and prevents the degradation of multiple client proteins. One of these HSP90 clients is BCR-ABL, the oncoprotein characteristic of chronic myeloid leukemia (CML) and the target of tyrosine kinase inhibitors, such as imatinib. Alvespimycin is an HSP90 inhibitor with better pharmacokinetic properties and fewer side effects than other similar drugs, but its role in overcoming imatinib resistance is not yet clarified. This work studied the therapeutic potential of alvespimycin in imatinib-sensitive (K562) and imatinib-resistant (K562-RC and K562-RD) CML cell lines. Metabolic activity was determined by the resazurin assay. Cell death, caspase activity, mitochondrial membrane potential, and cell cycle were evaluated by means of flow cytometry. Cell death was also analyzed by optical microscopy. HSPs expression levels were assessed by western blotting. Alvespimycin reduced metabolic activity in a time-, dose-, and cell line-dependent manner. Resistant cells were more sensitive to alvespimycin with an IC
MeSH term(s)	Humans ; Antineoplastic Agents/pharmacology ; Antineoplastic Agents/therapeutic use ; Apoptosis ; Cell Line, Tumor ; Drug Resistance, Neoplasm ; Fusion Proteins, bcr-abl/genetics ; Fusion Proteins, bcr-abl/metabolism ; Heat-Shock Proteins ; Imatinib Mesylate/pharmacology ; Imatinib Mesylate/therapeutic use ; K562 Cells ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/drug therapy ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/metabolism ; HSP90 Heat-Shock Proteins/metabolism
Chemical Substances	17-(dimethylaminoethylamino)-17-demethoxygeldanamycin (001L2FE0M3) ; Antineoplastic Agents ; Fusion Proteins, bcr-abl (EC 2.7.10.2) ; Heat-Shock Proteins ; Imatinib Mesylate (8A1O1M485B) ; HSP90 Heat-Shock Proteins
Language	English
Publishing date	2023-01-26
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules28031210
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.MO 81: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Platelet Membrane Proteins as Pain Biomarkers in Patients with Severe Dementia.

Ribeiro, Hugo / Alves, Raquel / Jorge, Joana / Gonçalves, Ana Cristina / Sarmento-Ribeiro, Ana Bela / Teixeira-Veríssimo, Manuel / Dourado, Marília / Andrade, José Paulo

Biomedicines

2023 Volume 11, Issue 2

Abstract: Pain is one of the most frequent health problems, and its evaluation and therapeutic approach largely depend on patient self-report. When it is not possible to obtain a self-report, the therapeutic decision becomes more difficult and limited. This study ... ...

Abstract	Pain is one of the most frequent health problems, and its evaluation and therapeutic approach largely depend on patient self-report. When it is not possible to obtain a self-report, the therapeutic decision becomes more difficult and limited. This study aims to evaluate whether some membrane platelet proteins could be of value in pain characterization. To achieve this goal, we used 53 blood samples obtained from palliative patients, 44 with non-oncological pain and nine without pain. We observed in patients with pain a decrease in the percentage of platelets expressing CD36, CD49f, and CD61 and in the expression levels of CD49f and CD61 when compared with patients without pain. Besides that, an increase in the percentage of platelets expressing CD62p was observed in patients with pain. These results suggest that the levels of these platelet cluster differentiations (CDs) could have some value as pain biomarkers objectively since they are not dependent on the patient's participation. Likewise, CD40 seems to have some importance as a biomarker of moderate and/or severe pain. The identification of pain biomarkers such as CD40, CD49f, CD62p and CD61 can lead to an adjustment of the therapeutic strategy, contributing to a faster and more adequate control of pain and reduction in patient-associated suffering.
Language	English
Publishing date	2023-01-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720867-9
ISSN	2227-9059
ISSN	2227-9059
DOI	10.3390/biomedicines11020380
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Monocytes in the Characterization of Pain in Palliative Patients with Severe Dementia-A Pilot Study.

Ribeiro, Hugo / Alves, Raquel / Jorge, Joana / Gonçalves, Ana Cristina / Sarmento-Ribeiro, Ana Bela / Teixeira-Veríssimo, Manuel / Andrade, José Paulo / Dourado, Marília

International journal of molecular sciences

2023 Volume 24, Issue 13

Abstract: In assessing and managing pain, when obtaining a self-report is impossible, therapeutic decision-making becomes more challenging. This study aimed to investigate whether monocytes and some membrane monocyte proteins, identified as a cluster of ... ...

Abstract	In assessing and managing pain, when obtaining a self-report is impossible, therapeutic decision-making becomes more challenging. This study aimed to investigate whether monocytes and some membrane monocyte proteins, identified as a cluster of differentiation (CD), could be potential non-invasive peripheral biomarkers in identifying and characterizing pain in patients with severe dementia. We used 53 blood samples from non-oncological palliative patients, 44 patients with pain (38 of whom had dementia) and 0 without pain or dementia (controls). We evaluated the levels of monocytes and their subtypes, including classic, intermediate, and non-classic, and characterized the levels of specific phenotypic markers, namely CD11c, CD86, CD163, and CD206. We found that the relative concentrations of monocytes, particularly the percentage of classic monocytes, may be a helpful pain biomarker. Furthermore, the CD11c expression levels were significantly higher in patients with mixed pain, while CD163 and CD206 expression levels were significantly higher in patients with nociceptive pain. These findings suggest that the levels of monocytes, particularly the classic subtype, and their phenotype markers CD11c, CD163, and CD206 could serve as pain biomarkers in patients with severe dementia.
MeSH term(s)	Humans ; Monocytes/metabolism ; Pilot Projects ; Antigens, Differentiation, Myelomonocytic/metabolism ; Biomarkers/metabolism ; Membrane Proteins/metabolism ; Pain/metabolism ; Dementia/complications ; Dementia/metabolism
Chemical Substances	Antigens, Differentiation, Myelomonocytic ; Biomarkers ; Membrane Proteins
Language	English
Publishing date	2023-06-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms241310723
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Zinc Prevents DNA Damage in Normal Cells but Shows Genotoxic and Cytotoxic Effects in Acute Myeloid Leukemia Cells.

Costa, Maria Inês / Lapa, Beatriz Santos / Jorge, Joana / Alves, Raquel / Carreira, Isabel Marques / Sarmento-Ribeiro, Ana Bela / Gonçalves, Ana Cristina

International journal of molecular sciences

2022 Volume 23, Issue 5

Abstract: Genomic instability is prevented by the DNA damage response (DDR). Micronutrients, like zinc (Zn), are cofactors of DDR proteins, and micronutrient deficiencies have been related to increased cancer risk. Acute myeloid leukemia (AML) patients commonly ... ...

Abstract	Genomic instability is prevented by the DNA damage response (DDR). Micronutrients, like zinc (Zn), are cofactors of DDR proteins, and micronutrient deficiencies have been related to increased cancer risk. Acute myeloid leukemia (AML) patients commonly present Zn deficiency. Moreover, reports point to DDR defects in AML. We studied the effects of Zn in DDR modulation in AML. Cell lines of AML (HEL) and normal human lymphocytes (IMC) were cultured in standard culture, Zn depletion, and supplementation (40 μM ZnSO
MeSH term(s)	Antineoplastic Agents ; DNA Damage ; Humans ; Hydrogen Peroxide/toxicity ; Leukemia, Myeloid, Acute/genetics ; Micronucleus Tests ; Zinc/pharmacology
Chemical Substances	Antineoplastic Agents ; Hydrogen Peroxide (BBX060AN9V) ; Zinc (J41CSQ7QDS)
Language	English
Publishing date	2022-02-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms23052567
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Urate-lowering effect of calcium supplementation: Analyses of a randomized controlled trial.

Cândido, Flávia Galvão / Alves, Raquel Duarte Moreira / Freitas, Dayse Mara de Oliveira / Bittencourt, Jersica Martins / Rocha, Daniela Mayumi Usuda Prado / Alfenas, Rita de Cássia Gonçalves

Clinical nutrition ESPEN

2022 Volume 49, Page(s) 86–91

Abstract: Objective: To investigate if the gout-protective effect of low-fat dairy products could be attributed to the urate-lowering effect of calcium.: Methods: This is a placebo-controlled trial in which thirty-five adult (aged 18-42 years) female low- ... ...

Abstract	Objective: To investigate if the gout-protective effect of low-fat dairy products could be attributed to the urate-lowering effect of calcium. Methods: This is a placebo-controlled trial in which thirty-five adult (aged 18-42 years) female low-calcium consumers (<800 mg/d) were randomized to one of three treatment groups: low calcium breakfast (control, ∼70 mg of calcium/d) -C or high-calcium breakfast (∼770 mg/d) from calcium citrate - CIT or from skim milk - SM, during 45 consecutive days. Breakfasts were matched for potential confounders and were provided as part of an energy-restricted normoprotein diet containing an additional 800 mg of calcium/d. Dual-energy X-ray absorptiometry measurements (body fat assessment) and fasting blood samples (urate, ionic calcium, PTH, and 1,25-(OH)2-D3) were taken at baseline and the end of the experiment. Clinical trial registration: http://www.ensaiosclinicos.gov.br/ (RBR-7Q2N33). Results: Despite no significant changes in total body weight/fat, CIT and SM led to a significant reduction in serum urate and ionic calcium, but did not affect PTH and vitamin D concentrations compared to C. CIT and SM reduced baseline serum urate by ∼14% and ∼17%, respectively. There was a trend to a positive correlation between changes in serum urate and changes in ionic calcium on day 45 (r = 0.327, P = 0.055). Conclusions: Calcium supplementation (770 mg/d from dairy or calcium citrate) reduced serum urate concentrations, suggesting that the gout-protective effect of low-fat dairy consumption is at least partly due to a urate-lowering effect of calcium.
MeSH term(s)	Adult ; Calcium ; Calcium Citrate/pharmacology ; Calcium, Dietary ; Dietary Supplements ; Female ; Gout ; Humans ; Uric Acid
Chemical Substances	Calcium, Dietary ; Uric Acid (268B43MJ25) ; Calcium Citrate (MLM29U2X85) ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2022-03-09
Publishing country	England
Document type	Journal Article ; Randomized Controlled Trial ; Research Support, U.S. Gov't, Non-P.H.S.
ISSN	2405-4577
ISSN (online)	2405-4577
DOI	10.1016/j.clnesp.2022.02.121
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Urate-lowering effect of calcium supplementation: Analyses of a randomized controlled trial

Cândido, Flávia Galvão / Alves, Raquel Duarte Moreira / Freitas, Dayse Mara de Oliveira / Bittencourt, Jersica Martins / Rocha, Daniela Mayumi Usuda Prado / Alfenas, Rita de Cássia Gonçalves

European Society for Clinical Nutrition and Metabolism Clinical Nutrition ESPEN. 2022 June, v. 49 p.86-91

2022

Abstract: To investigate if the gout-protective effect of low-fat dairy products could be attributed to the urate-lowering effect of calcium. This is a placebo-controlled trial in which thirty-five adult (aged 18–42 years) female low-calcium consumers (<800 mg/d) ... ...

Abstract	To investigate if the gout-protective effect of low-fat dairy products could be attributed to the urate-lowering effect of calcium. This is a placebo-controlled trial in which thirty-five adult (aged 18–42 years) female low-calcium consumers (<800 mg/d) were randomized to one of three treatment groups: low calcium breakfast (control, ∼70 mg of calcium/d) –C or high-calcium breakfast (∼770 mg/d) from calcium citrate – CIT or from skim milk – SM, during 45 consecutive days. Breakfasts were matched for potential confounders and were provided as part of an energy-restricted normoprotein diet containing an additional 800 mg of calcium/d. Dual-energy X-ray absorptiometry measurements (body fat assessment) and fasting blood samples (urate, ionic calcium, PTH, and 1,25-(OH)2-D3) were taken at baseline and the end of the experiment. Clinical trial registration: http://www.ensaiosclinicos.gov.br/ (RBR-7Q2N33). Despite no significant changes in total body weight/fat, CIT and SM led to a significant reduction in serum urate and ionic calcium, but did not affect PTH and vitamin D concentrations compared to C. CIT and SM reduced baseline serum urate by ∼14% and ∼17%, respectively. There was a trend to a positive correlation between changes in serum urate and changes in ionic calcium on day 45 (r = 0.327, P = 0.055). Calcium supplementation (770 mg/d from dairy or calcium citrate) reduced serum urate concentrations, suggesting that the gout-protective effect of low-fat dairy consumption is at least partly due to a urate-lowering effect of calcium.
Keywords	adults ; blood serum ; body fat ; body weight ; breakfast ; calcium ; calcium citrate ; clinical nutrition ; dairy consumption ; dual-energy X-ray absorptiometry ; females ; low fat dairy products ; randomized clinical trials ; skim milk ; Dairy products ; Milky ; PTH ; Vitamin D ; Uricemia ; LC ; CIT ; SM
Language	English
Dates of publication	2022-06
Size	p. 86-91.
Publishing place	Elsevier Ltd
Document type	Article ; Online
ISSN	2405-4577
DOI	10.1016/j.clnesp.2022.02.121
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED