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  1. Article: Detection by multiplex PCR of Mycoplasma species associated with dairy cattle in Argentina.

    Neder, Verónica E / Amadio, Ariel F / Calvinho, Luis F

    Revista Argentina de microbiologia

    2021  Volume 54, Issue 2, Page(s) 158–161

    Abstract: There is scarce information about the frequency and epidemiological and clinical features associated with the presence of Mycoplasma spp. in Argentine dairy herds. The objectives of this study were to develop a multiplex PCR for identifying M.bovis and M. ...

    Abstract There is scarce information about the frequency and epidemiological and clinical features associated with the presence of Mycoplasma spp. in Argentine dairy herds. The objectives of this study were to develop a multiplex PCR for identifying M.bovis and M.canadense and to describe the frequency of Mycoplasma spp. isolated from clinical samples submitted to a diagnostic laboratory. Of a total of 1548 samples from intramammary infections, bulk tank milk and biological fluids, 38 Mycoplasma isolates were obtained. M. bovis, M. canadense, M.californicum and M.leachii were detected by using two multiplex PCRs, confirming their presence in clinical conditions in dairy cattle. The techniques used in the present study can be useful to broaden the knowledge about Mycoplasma infections in cattle, since the search for these organisms is not usually included in routine diagnoses.
    MeSH term(s) Animals ; Argentina/epidemiology ; Cattle ; Cattle Diseases/diagnosis ; Cattle Diseases/epidemiology ; Female ; Mastitis, Bovine ; Milk ; Multiplex Polymerase Chain Reaction ; Mycoplasma/genetics ; Mycoplasma Infections/diagnosis ; Mycoplasma Infections/epidemiology ; Mycoplasma Infections/veterinary
    Language English
    Publishing date 2021-09-04
    Publishing country Argentina
    Document type Journal Article
    ZDB-ID 731952-6
    ISSN 0325-7541
    ISSN 0325-7541
    DOI 10.1016/j.ram.2021.07.001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4243: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article: Identification of key microorganisms in facultative stabilization ponds from dairy industries, using metagenomics.

    Irazoqui, Jose M / Eberhardt, Maria F / Adjad, Maria M / Amadio, Ariel F

    PeerJ

    2022  Volume 10, Page(s) e12772

    Abstract: Wastewater stabilization ponds are a natural form of wastewater treatment. Their low operation and maintenance costs have made them popular, especially in developing countries. In these systems, effluents are retained for long periods of time, allowing ... ...

    Abstract Wastewater stabilization ponds are a natural form of wastewater treatment. Their low operation and maintenance costs have made them popular, especially in developing countries. In these systems, effluents are retained for long periods of time, allowing the microbial communities present in the ponds to degrade the organic matter present, using both aerobic and anaerobic processes. Even though these systems are widespread in low income countries, there are no studies about the microorganisms present in them and how they operate. In this study, we analised the microbial communities of two serial full-scale stabilization ponds systems using whole genome shotgun sequencing. First, a taxonomic profiling of the reads was performed, to estimate the microbial diversity. Then, the reads of each system were assembled and binned, allowing the reconstruction of 110 microbial genomes. A functional analysis of the genomes allowed us to find how the main metabolic pathways are carried out, and we propose several organisms that would be key to this kind of environment, since they play an important role in these metabolic pathways. This study represents the first genome-centred approach to understand the metabolic processes in facultative ponds. A better understanding of these microbial communities and how they stabilize the effluents of dairy industries is necessary to improve them and to minimize the environmental impact of dairy industries wastewater.
    Language English
    Publishing date 2022-03-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2703241-3
    ISSN 2167-8359
    ISSN 2167-8359
    DOI 10.7717/peerj.12772
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Detection by multiplex PCR of Mycoplasma species associated with dairy cattle in Argentina

    Neder, Verónica E. / Amadio, Ariel F. / Calvinho, Luis F.

    Asociación Argentina de Microbiología Revista argentina microbiología. 2021 July 04,

    2021  

    Abstract: There is scarce information about the frequency and epidemiological and clinical features associated with the presence of Mycoplasma spp. in Argentine dairy herds. The objectives of this study were to develop a multiplex PCR for identifying M.bovis and M. ...

    Abstract There is scarce information about the frequency and epidemiological and clinical features associated with the presence of Mycoplasma spp. in Argentine dairy herds. The objectives of this study were to develop a multiplex PCR for identifying M.bovis and M.canadense and to describe the frequency of Mycoplasma spp. isolated from clinical samples submitted to a diagnostic laboratory. Of a total of 1548 samples from intramammary infections, bulk tank milk and biological fluids, 38 Mycoplasma isolates were obtained. M. bovis, M. canadense, M.californicum and M.leachii were detected by using two multiplex PCRs, confirming their presence in clinical conditions in dairy cattle. The techniques used in the present study can be useful to broaden the knowledge about Mycoplasma infections in cattle, since the search for these organisms is not usually included in routine diagnoses.
    Keywords bulk milk ; dairy cattle ; polymerase chain reaction ; Argentina
    Language English
    Dates of publication 2021-0704
    Publishing place Elsevier España, S.L.U.
    Document type Article
    Note Pre-press version
    ZDB-ID 731952-6
    ISSN 0325-7541
    ISSN 0325-7541
    DOI 10.1016/j.ram.2021.07.001
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4243: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Reconstruction and Characterization of Full-Length Begomovirus and Alphasatellite Genomes Infecting Pepper through Metagenomics.

    Bornancini, Verónica A / Irazoqui, José M / Flores, Ceferino R / Vaghi Medina, Carlos G / Amadio, Ariel F / López Lambertini, Paola M

    Viruses

    2020  Volume 12, Issue 2

    Abstract: In northwestern Argentina (NWA), pepper crops are threatened by the emergence of begomoviruses due to the spread of its vector, ...

    Abstract In northwestern Argentina (NWA), pepper crops are threatened by the emergence of begomoviruses due to the spread of its vector,
    MeSH term(s) Begomovirus/classification ; Begomovirus/genetics ; Capsicum/virology ; DNA, Viral/genetics ; Genetic Variation ; Genome, Viral ; High-Throughput Nucleotide Sequencing ; Leonurus/virology ; Metagenomics/methods ; Phylogeny ; Plant Diseases/virology ; Recombination, Genetic ; Satellite Viruses/genetics ; Sequence Analysis, DNA
    Chemical Substances DNA, Viral
    Language English
    Publishing date 2020-02-11
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v12020202
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Design and evaluation of synthetic bacterial consortia for optimized phenanthrene degradation through the integration of genomics and shotgun proteomics.

    Macchi, Marianela / Festa, Sabrina / Nieto, Esteban / Irazoqui, José M / Vega-Vela, Nelson E / Junca, Howard / Valacco, María P / Amadio, Ariel F / Morelli, Irma S / Coppotelli, Bibiana M

    Biotechnology reports (Amsterdam, Netherlands)

    2021  Volume 29, Page(s) e00588

    Abstract: Two synthetic bacterial consortia (SC) composed of bacterial ... ...

    Abstract Two synthetic bacterial consortia (SC) composed of bacterial strains
    Language English
    Publishing date 2021-01-06
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2801018-8
    ISSN 2215-017X
    ISSN 2215-017X
    DOI 10.1016/j.btre.2021.e00588
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: L-cysteine transporter-PCR to detect hydrogen sulfide-producing

    Farace, Pablo D / Morsella, Claudia G / Cravero, Silvio L / Sioya, Bernardo A / Amadio, Ariel F / Paolicchi, Fernando A / Gioffré, Andrea K

    PeerJ

    2019  Volume 7, Page(s) e7820

    Abstract: Phenotypic differences ... ...

    Abstract Phenotypic differences between
    Language English
    Publishing date 2019-11-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2703241-3
    ISSN 2167-8359
    ISSN 2167-8359
    DOI 10.7717/peerj.7820
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: First Genome Sequence of Leptospira interrogans Serovar Pomona, Isolated from a Bovine Abortion.

    Varni, Vanina / Koval, Ariel / Nagel, Ariel / Ruybal, Paula / Caimi, Karina / Amadio, Ariel F

    Genome announcements

    2016  Volume 4, Issue 3

    Abstract: Leptospirosis is a widespread zoonosis and a re-emergent disease of global distribution with major relevance in veterinary production. Here, we report the whole-genome sequence of Leptospira interrogans serovar Pomona strain AKRFB, isolated from a bovine ...

    Abstract Leptospirosis is a widespread zoonosis and a re-emergent disease of global distribution with major relevance in veterinary production. Here, we report the whole-genome sequence of Leptospira interrogans serovar Pomona strain AKRFB, isolated from a bovine abortion during a leptospirosis outbreak in Argentina.
    Language English
    Publishing date 2016-05-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2704277-7
    ISSN 2169-8287
    ISSN 2169-8287
    DOI 10.1128/genomeA.00345-16
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Draft Genome Sequence of

    Navas, Laura E / Berretta, Marcelo F / Ortiz, Elio M / Sauka, Diego H / Benintende, Graciela B / Zandomeni, Rubén O / Amadio, Ariel F

    Genome announcements

    2017  Volume 5, Issue 13

    Abstract: We report here the complete annotated 6,035,547-bp draft genome sequence ... ...

    Abstract We report here the complete annotated 6,035,547-bp draft genome sequence of
    Language English
    Publishing date 2017-03-30
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2704277-7
    ISSN 2169-8287
    ISSN 2169-8287
    DOI 10.1128/genomeA.00076-17
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Broad-range lytic bacteriophages that kill Staphylococcus aureus local field strains.

    Abatángelo, Virginia / Peressutti Bacci, Natalia / Boncompain, Carina A / Amadio, Ariel F / Carrasco, Soledad / Suárez, Cristian A / Morbidoni, Héctor R

    PloS one

    2017  Volume 12, Issue 7, Page(s) e0181671

    Abstract: Staphylococcus aureus is a very successful opportunistic pathogen capable of causing a variety of diseases ranging from mild skin infections to life-threatening sepsis, meningitis and pneumonia. Its ability to display numerous virulence mechanisms ... ...

    Abstract Staphylococcus aureus is a very successful opportunistic pathogen capable of causing a variety of diseases ranging from mild skin infections to life-threatening sepsis, meningitis and pneumonia. Its ability to display numerous virulence mechanisms matches its skill to display resistance to several antibiotics, including β-lactams, underscoring the fact that new anti-S. aureus drugs are urgently required. In this scenario, the utilization of lytic bacteriophages that kill bacteria in a genus -or even species- specific way, has become an attractive field of study. In this report, we describe the isolation, characterization and sequencing of phages capable of killing S. aureus including methicillin resistant (MRSA) and multi-drug resistant S. aureus local strains from environmental, animal and human origin. Genome sequencing and bio-informatics analysis showed the absence of genes encoding virulence factors, toxins or antibiotic resistance determinants. Of note, there was a high similarity between our set of phages to others described in the literature such as phage K. Considering that reported phages were obtained in different continents, it seems plausible that there is a commonality of genetic features that are needed for optimum, broad host range anti-staphylococcal activity of these related phages. Importantly, the high activity and broad host range of one of our phages underscores its promising value to control the presence of S. aureus in fomites, industry and hospital environments and eventually on animal and human skin. The development of a cocktail of the reported lytic phages active against S. aureus-currently under way- is thus, a sensible strategy against this pathogen.
    MeSH term(s) Bacteriophages/genetics ; Bacteriophages/isolation & purification ; Bacteriophages/physiology ; Genome, Viral/genetics ; Methicillin-Resistant Staphylococcus aureus/virology ; Microscopy, Electron, Transmission ; Myoviridae/genetics ; Myoviridae/physiology ; Sequence Analysis, DNA ; Staphylococcus aureus/virology
    Language English
    Publishing date 2017
    Publishing country United States
    Document type Journal Article
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0181671
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Correction: Broad-range lytic bacteriophages that kill Staphylococcus aureus local field strains.

    Abatángelo, Virginia / Peressutti Bacci, Natalia / Boncompain, Carina A / Amadio, Ariel F / Carrasco, Soledad / Suárez, Cristian A / Morbidoni, Héctor R

    PloS one

    2017  Volume 12, Issue 10, Page(s) e0187387

    Abstract: This corrects the article DOI: 10.1371/journal.pone.0181671.]. ...

    Abstract [This corrects the article DOI: 10.1371/journal.pone.0181671.].
    Language English
    Publishing date 2017
    Publishing country United States
    Document type Journal Article ; Published Erratum
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0187387
    Database MEDical Literature Analysis and Retrieval System OnLINE

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