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  1. Article ; Online: Influence of sugars and osmoregulators on motility and viability of cooled and frozen-thawed ram semen

    Khaled El-Shahat / Magdi Waheed / Amal Hasan Ali / Abdel -Aziz Sallam / Badr El-Saidy

    Roczniki Naukowe Polskiego Towarzystwa Zootechnicznego, Vol 16, Iss 2, Pp 51-

    2020  Volume 57

    Abstract: The aim of the study was to determine the effect of sugars and osmoregulators on the viability of frozen-thawed ram semen in a Tris-based diluent. Two experiments were conducted to evaluate the effect of sugars (fructose, glucose, sucrose and raffinose) ... ...

    Abstract The aim of the study was to determine the effect of sugars and osmoregulators on the viability of frozen-thawed ram semen in a Tris-based diluent. Two experiments were conducted to evaluate the effect of sugars (fructose, glucose, sucrose and raffinose) and two osmoregulators (hypotaurine and taurine) on the viability of ram sperm. In experiment 1, each of the four sugars (fructose, glucose, sucrose and raffinose) was added (500 mg%) to the diluents. Semen was collected from 15 rams once a week using an artificial vagina, diluted, cooled slowly to 5°C over 2 h, frozen in the form of pellets, and plunged into liquid nitrogen. The frozen semen was thawed, and sperm viability was calculated 3 h after thawing. In experiment 2, two osmoregulators, hypotaurine (0.20 and 0.40 mg/ml) and taurine (2 and 4 mg/ml), were added to Tris-based raffinose diluent. Motility and viability rates were calculated. The results showed that motility was gradually significantly (P < 0.05) improved by using 500 mg% raffinose in the Tris-based diluents after dilution at 30°C and before freezing at 5°C. Post-thawing motility and viability rates were highest (P<0.05) when raffinose was used in Tris-based diluent for cryopreservation of ram semen. In vitro supplementation of the semen diluent with 4 mg/ml taurine had a beneficial effect on the motility and viability of ram spermatozoa after dilution at 30°C and before freezing at 5°C. The same trend was observed after freezing-thawing and 3 h post-thawing. In conclusion, 500 mg% raffinose was a more suitable sugar component for treatment of ram spermatozoa in Tris diluent than fructose or glucose, while 4 mg/ml of taurine in Tris-raffinose medium exerted a beneficial effect on the motility and viability of ram sperm at cooling and post-thawing.
    Keywords Ram semen ; Sugars ; Osmoregulator ; Dilution ; Freezing ; Zoology ; QL1-991
    Subject code 630
    Language English
    Publishing date 2020-06-01T00:00:00Z
    Publisher Polish Society of Animal Production
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Association between Adipose Tissue Interleukin-33 and Immunometabolic Markers in Individuals with Varying Degrees of Glycemia

    Amal Hasan / Shihab Kochumon / Ebaa Al-Ozairi / Jaakko Tuomilehto / Rasheed Ahmad

    Disease Markers, Vol

    2019  Volume 2019

    Abstract: Introduction. Interleukin-33 (IL-33), the ligand for the receptor ST2, is abundant in adipose tissue, including preadipocytes, adipocytes, and endothelial cells. The IL-33/ST2 axis is protective against obesity, insulin resistance, and type 2 diabetes ( ... ...

    Abstract Introduction. Interleukin-33 (IL-33), the ligand for the receptor ST2, is abundant in adipose tissue, including preadipocytes, adipocytes, and endothelial cells. The IL-33/ST2 axis is protective against obesity, insulin resistance, and type 2 diabetes (T2D) in animal models. We determined whether adipose tissue IL-33 was associated with glycated hemoglobin (HbA1c), as well as mediators of inflammation and immune regulation and beiging of adipose tissue, among individuals with varying degrees of glycemia. Materials and Methods. A total of 91 adults with normoglycemia, prediabetes, and T2D were included. After measuring their anthropometric and biochemical parameters, subcutaneous adipose tissue samples were isolated and mRNA expression of cytokines, chemokines, chemokine receptors, pattern recognition receptors, and mediators involved in beiging of adipose tissue were measured. Results. Adipose tissue IL-33 was inversely associated with HbA1c in individuals with normoglycemia and T2D but not in those with prediabetes and was inversely correlated with fasting plasma glucose in individuals with T2D and with a better glycemic control. IL-33-to-ST2 ratio was inversely correlated with HbA1c in individuals with normoglycemia but not in those with prediabetes or T2D. IL-33 was directly associated with ST2, CD302, fibrinogen-like protein 2 (FGL2), and PR domain containing 16 (PRDM16) but inversely correlated with chemokine (C-C motif) ligand (CCL) 7 and CCL8 in individuals with normoglycemia. Similarly, IL-33 was directly associated with ST2, CD302, FGL2, PRDM16, and, additionally, toll-like receptor (TLR) 3 and IL-12A in individuals with T2D. However, IL-33 was not associated with any of these mediators but was directly and strongly associated with TLR9 in individuals with prediabetes. Conclusions. IL-33 and/or IL-33/ST2 dynamics and biological functions may play a role in overall glycemia among humans and may represent a novel target by which glucose-lowering managements confer their beneficial effects.
    Keywords Medicine (General) ; R5-920
    Subject code 571 ; 616
    Language English
    Publishing date 2019-01-01T00:00:00Z
    Publisher Hindawi Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Association of COVID-19 Vaccines ChAdOx1-S and BNT162b2 with Circulating Levels of Coagulation Factors and Antithrombin

    Amal Hasan / Hossein Arefanian / Arshad Mohamed Channanath / Irina AlKhairi / Preethi Cherian / Sriraman Devarajan / Thangavel Alphonse Thanaraj / Mohamed Abu-Farha / Jehad Abubaker / Fahd Al-Mulla

    Vaccines, Vol 10, Iss 8, p

    2022  Volume 1226

    Abstract: Background: Severe coronavirus disease 2019 (COVID-19) is associated with increased risk of thrombosis and thromboembolism. Exposure to COVID-19 vaccines is also associated with immune thrombotic thrombocytopenia, ischemic stroke, intracerebral ... ...

    Abstract Background: Severe coronavirus disease 2019 (COVID-19) is associated with increased risk of thrombosis and thromboembolism. Exposure to COVID-19 vaccines is also associated with immune thrombotic thrombocytopenia, ischemic stroke, intracerebral haemorrhage, and cerebral venous thrombosis, and it is linked with systemic activation of coagulation. Methods: We assess the circulating levels of coagulation factors (factors XI, XII, XIII, and prothrombin) and antithrombin in individuals who completed two doses of either ChAdOx1-S or BNT162b2 COVID-19 vaccine, within the timeframe of two months, who had no previous history of COVID-19. Results: Elevated levels of factors XI, XII, XIII, prothrombin, and antithrombin were seen compared to unvaccinated controls. Levels of coagulation factors, antithrombin, and prothrombin to antithrombin ratio were higher with BNT162b2 compared to ChAdOx1-S vaccine. Conclusions: The clinical significance of such coagulation homeostasis disruption remains to be elucidated but it is worthy of global scientific follow-up effort.
    Keywords COVID-19 vaccines ; coagulation factors ; antithrombin ; Medicine ; R
    Subject code 610
    Language English
    Publishing date 2022-07-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Stearic Acid and TNF-α Co-Operatively Potentiate MIP-1α Production in Monocytic Cells via MyD88 Independent TLR4/TBK/IRF3 Signaling Pathway

    Shihab Kochumon / Hossein Arefanian / Rafaat Azim / Steve Shenouda / Texy Jacob / Nermeen Abu Abu Khalaf / Fatema Al-Rashed / Amal Hasan / Sardar Sindhu / Fahd Al-Mulla / Rasheed Ahmad

    Biomedicines, Vol 8, Iss 403, p

    2020  Volume 403

    Abstract: Increased circulatory and adipose tissue expression of macrophage inflammatory protein (MIP)-1α (CC motif chemokine ligand-3/CCL3) and its association with inflammation in the state of obesity is well documented. Since obesity is associated with ... ...

    Abstract Increased circulatory and adipose tissue expression of macrophage inflammatory protein (MIP)-1α (CC motif chemokine ligand-3/CCL3) and its association with inflammation in the state of obesity is well documented. Since obesity is associated with increases in both stearic acid and tumor necrosis factor α (TNF-α) in circulation, we investigated whether stearic acid and TNF-α together could regulate MIP-1α/CCL3 expression in human monocytic cells, and if so, which signaling pathways were involved in MIP-1α/CCL3 modulation. Monocytic cells were treated with stearic acid and TNF-α resulted in enhanced production of MIP-1α/CCL3 compared to stearic acid or TNF-α alone. To explore the underlying mechanisms, cooperative effect of stearic acid for MIP-α/CCL3 expression was reduced by TLR4 blocking, and unexpectedly we found that the synergistic production of MIP-α/CCL3 in MyD88 knockout (KO) cells was not suppressed. In contrast, this MIP-α/CCL3 expression was attenuated by inhibiting TBK1/IRF3 activity. Cells deficient in IRF3 did not show cooperative effect of stearate/TNF-α on MIP-1α/CCL3 production. Furthermore, activation of IRF3 by polyinosinic-polycytidylic acid (poly I:C) produced a cooperative effect with TNF-α for MIP-1α/CCL3 production that was comparable to stearic acid. Individuals with obesity show high IRF3 expression in monocytes as compared to lean individuals. Furthermore, elevated levels of MIP-1α/CCL3 positively correlate with TNF-α and CD163 in fat tissues from individuals with obesity. Taken together, this study provides a novel model for the pathologic role of stearic acid to produce MIP-1α/CCL3 in the presence of TNF-α associated with obesity settings.
    Keywords MIP-1α ; stearic acid ; TNF-α/CCL3 ; TLR4 ; TRIF/TBK ; IRF3 ; Biology (General) ; QH301-705.5
    Subject code 570
    Language English
    Publishing date 2020-10-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Dynamics of Anti-S IgG Antibodies Titers after the Second Dose of COVID-19 Vaccines in the Manual and Craft Worker Population of Qatar

    Devendra Bansal / Hassan Atia / Mashael Al Badr / Mohamed Nour / Jazeel Abdulmajeed / Amal Hasan / Noora Al-Hajri / Lina Ahmed / Rumissa Ibrahim / Reham Zamel / Almuthana Mohamed / Hamad Pattalaparambil / Faisal Daraan / Adil Chaudhry / Sahar Oraby / Sahar El-Saleh / Sittana S. El-Shafie / Affra Faiz Al-Farsi / Jiji Paul /
    Ahmed Ismail / Hamad Eid Al-Romaihi / Mohammed Hamad Al-Thani / Suhail A. R. Doi / Susu M. Zughaier / Farhan Cyprian / Elmobashar Farag / Habib Hasan Farooqui

    Vaccines, Vol 11, Iss 496, p

    2023  Volume 496

    Abstract: There is limited seroepidemiological evidence on the magnitude and long-term durability of antibody titers of mRNA and non-mRNA vaccines in the Qatari population. This study was conducted to generate evidence on long-term anti-S IgG antibody titers and ... ...

    Abstract There is limited seroepidemiological evidence on the magnitude and long-term durability of antibody titers of mRNA and non-mRNA vaccines in the Qatari population. This study was conducted to generate evidence on long-term anti-S IgG antibody titers and their dynamics in individuals who have completed a primary COVID-19 vaccination schedule. A total of 300 male participants who received any of the following vaccines BNT162b2/Comirnaty, mRNA-1273, ChAdOx1-S/Covishield, COVID-19 Vaccine Janssen/Johnson, or BBIBP-CorV or Covaxin were enrolled in our study. All sera samples were tested by chemiluminescent microparticle immunoassay (CMIA) for the quantitative determination of IgG antibodies to SARS-CoV-2, receptor-binding domain (RBD) of the S1 subunit of the spike protein of SARS-CoV-2. Antibodies against SARS-CoV-2 nucleocapsid (SARS-CoV-2 N-protein IgG) were also determined. Kaplan–Meier survival curves were used to compare the time from the last dose of the primary vaccination schedule to the time by which anti-S IgG antibody titers fell into the lowest quartile (range of values collected) for the mRNA and non-mRNA vaccines. Participants vaccinated with mRNA vaccines had higher median anti-S IgG antibody titers. Participants vaccinated with the mRNA-1273 vaccine had the highest median anti-S-antibody level of 13,720.9 AU/mL (IQR 6426.5 to 30,185.6 AU/mL) followed by BNT162b2 (median, 7570.9 AU/mL; IQR, 3757.9 to 16,577.4 AU/mL); while the median anti-S antibody titer for non-mRNA vaccinated participants was 3759.7 AU/mL (IQR, 2059.7–5693.5 AU/mL). The median time to reach the lowest quartile was 3.53 months (IQR, 2.2–4.5 months) and 7.63 months (IQR, 6.3–8.4 months) for the non-mRNA vaccine recipients and Pfizer vaccine recipients, respectively. However, more than 50% of the Moderna vaccine recipients did not reach the lowest quartile by the end of the follow-up period. This evidence on anti-S IgG antibody titers should be considered for informing decisions on the durability of the neutralizing activity and thus ...
    Keywords COVID-19 ; mRNA vaccines ; non-mRNA vaccines ; antibody titer ; anti-S IgG ; Medicine ; R
    Subject code 630
    Language English
    Publishing date 2023-02-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: TNF-α in Combination with Palmitate Enhances IL-8 Production via The MyD88- Independent TLR4 Signaling Pathway

    Amal Hasan / Nadeem Akhter / Areej Al-Roub / Reeby Thomas / Shihab Kochumon / Ajit Wilson / Merin Koshy / Ebaa Al-Ozairi / Fahd Al-Mulla / Rasheed Ahmad

    International Journal of Molecular Sciences, Vol 20, Iss 17, p

    Potential Relevance to Metabolic Inflammation

    2019  Volume 4112

    Abstract: Elevated levels of IL-8 (CXCL8) in obesity have been linked with insulin resistance and type 2 diabetes (T2D). The mechanisms that lead to the profound production of IL-8 in obesity remains to be understood. TNF-α and saturated free fatty acids (FFAs) ... ...

    Abstract Elevated levels of IL-8 (CXCL8) in obesity have been linked with insulin resistance and type 2 diabetes (T2D). The mechanisms that lead to the profound production of IL-8 in obesity remains to be understood. TNF-α and saturated free fatty acids (FFAs) are increased in obese humans and correlate with insulin resistance. Hence, we sought to investigate whether the cooccurrence of TNF-α and FFAs led to increase the production of IL-8 by human monocytes. We found that co-stimulation of human monocytes with palmitate and TNF-α led to increased IL-8 production as compared to those stimulated with palmitate or TNF-α alone. The synergistic production of IL-8 by TNF-α/palmitate was suppressed by neutralizing anti- Toll like receptor 4 (TLR4) antibody and by genetic silencing of TLR4. Both MyD88-deficient and MyD88-competent cells responded comparably to TNF-α/Palmitate. However, TIR-domain-containing adapter-inducing interferon (TRIF) inhibition or interferon regulatory transcription factor 3 (IRF3) knockdown partly blocked the synergistic production of IL-8. Our human data show that increased adipose tissue TNF-α expression correlated positively with IL-8 expression ( r = 0.49, P = 0.001). IL-8 and TNF-α correlated positively with macrophage markers including CD68, CD163 and CD86 in adipose tissue. These findings suggest that the signaling cross-talk between saturated fatty acid palmitate and TNF-α may be a key driver in obesity-associated chronic inflammation via an excessive production of IL-8.
    Keywords inflammation ; interleukin-8 ; tumor necrosis factor-alpha ; free fatty acids ; toll-like receptor 4 ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 571
    Language English
    Publishing date 2019-08-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Interaction of osteopontin with IL-18 in obese individuals

    Rasheed Ahmad / Anfal Al-Mass / Dalal Al-Ghawas / Nada Shareif / Nadia Zghoul / Motasem Melhem / Amal Hasan / Fahad Al-Ghimlas / Said Dermime / Kazem Behbehani

    PLoS ONE, Vol 8, Iss 5, p e

    implications for insulin resistance.

    2013  Volume 63944

    Abstract: BACKGROUND/OBJECTIVE: Osteopontin (OPN) and IL-18 are known inflammatory mediators and both participate in a wide range of biological processes linked to immunological disorders. Since an interaction between OPN and IL-18 has not been studied in obesity, ...

    Abstract BACKGROUND/OBJECTIVE: Osteopontin (OPN) and IL-18 are known inflammatory mediators and both participate in a wide range of biological processes linked to immunological disorders. Since an interaction between OPN and IL-18 has not been studied in obesity, we investigated whether: (i) their levels were simultaneously elevated in obese individuals; (ii) OPN was associated with IL-18 in obese individuals and (iii) their levels associated with fasting blood glucose (FBG) and BMI. SUBJECTS AND METHODS: PBMCs and plasma samples were isolated from 60 individuals including lean as well as overweight and obese individuals. Subcutaneous adipose tissue samples were obtained. OPN and IL-18 were measured by ELISA. OPN and IL-18 mRNA expression was quantified by real time quantitative RT-PCR. RESULTS: Obese individuals exhibited significantly increased circulating OPN levels as compared with lean individuals (obese 2865±101; lean 1681±116 pg/ml; P<0.0001). IL-18 levels were also high in obese individuals (obese 491±39, lean 301±26 pg/ml; P = 0.0009). OPN and IL-18 expression were simultaneously up-regulated (OPN: 5.4-Fold; IL-18: 8.9-Fold; P<0.05) in PBMCs from obese individuals compared to lean group. Adipose tissue from obese individuals had high expression of OPN (7.3-Fold) and IL-18 (9.6-Fold). Plasma OPN levels correlated positively with FBG levels (r = 0.32, P = 0.02). Similarly, IL-18 correlated positively with FBG levels (r = 0.406, P = 0.0042). Stepwise multiple regression analysis showed an independent association of BMI with OPN and IL-18. Interestingly, OPN levels increased progressively with an increase in IL-18 levels (r = 0.52, P = 0.0004). We also examined the regulatory role of IL-18 in OPN secretion from PBMCs. Neutralizing anti-IL-18Rα mAb reduced OPN secretion. CONCLUSION: These findings represent the first observation that plasma, PBMC and adipose tissue OPN and IL-18 are simultaneously increased and correlate with each other in overweight/obese individuals which may trigger the development of ...
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2013-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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