LIVIVO - Search results -

Search results

Result 1 - 10 of total 36

Search options

Article ; Online: Efficient and scalable clarification of Orf virus from HEK suspension for vaccine development.

Pagallies, Felix / Labisch, Jennifer J / Wronska, Malgorzata / Pflanz, Karl / Amann, Ralf

Vaccine: X

2024 Volume 18, Page(s) 100474

Abstract: The Orf virus (ORFV) is a promising vector platform for the generation of vaccines against infectious diseases and cancer, highlighted by its progression to clinical testing phases. One of the critical steps during GMP manufacturing is the clarification ... ...

Abstract	The Orf virus (ORFV) is a promising vector platform for the generation of vaccines against infectious diseases and cancer, highlighted by its progression to clinical testing phases. One of the critical steps during GMP manufacturing is the clarification of crude harvest because of the enveloped nature and large size of ORFV. This study presents the first description of ORFV clarification process from a HEK suspension batch process. We examined various filter materials, membrane pore sizes, harvest timings, and nuclease treatments. Employing the Ambr® crossflow system for high-throughput, small-volume experiments, we identified polypropylene-based Sartopure® PP3 filters as ideal. These filters, used in two consecutive stages with reducing pore sizes, significantly enhanced ORFV recovery and addressed scalability challenges. Moreover, we demonstrated that the time of harvest and the use of a nuclease play a decisive role to increase ORFV yields. With these findings, we were able to establish an efficient and scalable clarification process of ORFV derived from a suspension production process, essential for advancing ORFV vaccine manufacturing.
Language	English
Publishing date	2024-03-13
Publishing country	England
Document type	Journal Article
ISSN	2590-1362
ISSN (online)	2590-1362
DOI	10.1016/j.jvacx.2024.100474
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Orf Virus-Based Vectors Preferentially Target Professional Antigen-Presenting Cells, Activate the STING Pathway and Induce Strong Antigen-Specific T Cell Responses.

Müller, Melanie / Reguzova, Alena / Löffler, Markus W / Amann, Ralf

Frontiers in immunology

2022 Volume 13, Page(s) 873351

Abstract: Background: Orf virus (ORFV)-based vectors are attractive for vaccine development as they enable the induction of potent immune responses against specific transgenes. Nevertheless, the precise mechanisms of immune activation remain unknown. This study ... ...

Abstract	Background: Orf virus (ORFV)-based vectors are attractive for vaccine development as they enable the induction of potent immune responses against specific transgenes. Nevertheless, the precise mechanisms of immune activation remain unknown. This study therefore aimed to characterize underlying mechanisms in human immune cells. Methods: Peripheral blood mononuclear cells were infected with attenuated ORFV strain D1701-VrV and analyzed for ORFV infection and activation markers. ORFV entry in susceptible cells was examined using established pharmacological inhibitors. Using the THP1-Dual™ reporter cell line, activation of nuclear factor-κB and interferon regulatory factor pathways were simultaneously evaluated. Infection with an ORFV recombinant encoding immunogenic peptides (PepTrio-ORFV) was used to assess the induction of antigen-specific CD8+ T cells. Results: ORFV was found to preferentially target professional antigen-presenting cells (APCs) Discussion: Our findings confirm that ORFV induces a strong antigen-specific immune response dependent on APC uptake and activation. These data support the notion that ORFV D1701-VrV is a promising vector for vaccine development and the design of innovative immunotherapeutic applications.
MeSH term(s)	Antigen-Presenting Cells/immunology ; Humans ; Leukocytes, Mononuclear ; Membrane Proteins/immunology ; Membrane Proteins/metabolism ; Orf virus/genetics ; T-Lymphocytes/immunology ; Transgenes/genetics
Chemical Substances	Membrane Proteins ; STING1 protein, human
Language	English
Publishing date	2022-05-09
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2022.873351
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Pyroptosis and Its Role in SARS-CoV-2 Infection.

Bittner, Zsofia Agnes / Schrader, Markus / George, Shilpa Elizabeth / Amann, Ralf

Cells

2022 Volume 11, Issue 10

Abstract: The pore-forming inflammatory cell death pathway, pyroptosis, was first described in the early 1990s and its role in health and disease has been intensively studied since. The effector molecule GSDMD is cleaved by activated caspases, mainly Caspase 1 or ... ...

Abstract	The pore-forming inflammatory cell death pathway, pyroptosis, was first described in the early 1990s and its role in health and disease has been intensively studied since. The effector molecule GSDMD is cleaved by activated caspases, mainly Caspase 1 or 11 (Caspase 4/5 in humans), downstream of inflammasome formation. In this review, we describe the molecular events related to GSDMD-mediated pore formation. Furthermore, we summarize the so far elucidated ways of SARS-CoV-2 induced NLRP3 inflammasome formation leading to pyroptosis, which strongly contributes to COVID-19 pathology. We also explore the potential of NLRP3 and GSDMD inhibitors as therapeutics to counter excessive inflammation.
MeSH term(s)	COVID-19 ; Caspases/metabolism ; Humans ; Inflammasomes/metabolism ; Intracellular Signaling Peptides and Proteins/metabolism ; NLR Family, Pyrin Domain-Containing 3 Protein/metabolism ; Phosphate-Binding Proteins/metabolism ; Pyroptosis ; SARS-CoV-2
Chemical Substances	Inflammasomes ; Intracellular Signaling Peptides and Proteins ; NLR Family, Pyrin Domain-Containing 3 Protein ; Phosphate-Binding Proteins ; Caspases (EC 3.4.22.-)
Language	English
Publishing date	2022-05-23
Publishing country	Switzerland
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	2661518-6
ISSN	2073-4409 ; 2073-4409
ISSN (online)	2073-4409
ISSN	2073-4409
DOI	10.3390/cells11101717
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: An investigation of excipients for a stable Orf viral vector formulation.

Eilts, Friederike / Harsy, Yasmina M J / Lothert, Keven / Pagallies, Felix / Amann, Ralf / Wolff, Michael W

Virus research

2023 Volume 336, Page(s) 199213

Abstract: The Orf virus (ORFV) is a promising candidate for vector vaccines as well as for immunomodulatory and oncolytic therapies. However, few publications are available on its infectivity degradation or on suitable additives for prolonging its viral stability. ...

Abstract	The Orf virus (ORFV) is a promising candidate for vector vaccines as well as for immunomodulatory and oncolytic therapies. However, few publications are available on its infectivity degradation or on suitable additives for prolonging its viral stability. In this study, the non-supplemented ORFV itself showed a very high stability at storage temperatures up to 28 °C, with a linear titer loss of 0.10 log infectious particles per day at 4 °C over a period of five weeks. To prolong this inherent stability, thirty additives, i.e., detergents, sugars, proteins, salts, and buffers as well as amino acids, were tested for their time- and temperature-dependent influence on the ORFV infectivity. A stabilizing effect on the infectivity was identified for the addition of all tested proteins, i.e., gelatine, bovine serum albumin, and recombinant human serum albumin (rHSA), of several sugars, i.e., mannitol, galactose, sucrose, and trehalose, of amino acids, i.e., arginine and proline, of the detergent Pluronic F68, and of the salt Na
MeSH term(s)	Humans ; Excipients/chemistry ; Freeze Drying ; Proteins ; Sucrose/chemistry ; Sugars ; Amino Acids ; Arginine/chemistry
Chemical Substances	Excipients ; Proteins ; Sucrose (57-50-1) ; Sugars ; Amino Acids ; Arginine (94ZLA3W45F)
Language	English
Publishing date	2023-09-06
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605780-9
ISSN	1872-7492 ; 0168-1702
ISSN (online)	1872-7492
ISSN	0168-1702
DOI	10.1016/j.virusres.2023.199213
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1965: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: A Summary of Practical Considerations for the Application of the Steric Exclusion Chromatography for the Purification of the Orf Viral Vector.

Eilts, Friederike / Lothert, Keven / Orbay, Sabri / Pagallies, Felix / Amann, Ralf / Wolff, Michael W

Membranes

2022 Volume 12, Issue 11

Abstract: Steric exclusion chromatography (SXC) is a promising purification method for biological macromolecules such as the Orf virus (ORFV) vector. The method's principle is closely related to conventional polyethylene glycol (PEG) precipitation, repeatedly ... ...

Abstract	Steric exclusion chromatography (SXC) is a promising purification method for biological macromolecules such as the Orf virus (ORFV) vector. The method's principle is closely related to conventional polyethylene glycol (PEG) precipitation, repeatedly implementing membranes as porous chromatographic media. In the past decade, several purification tasks with SXC showed exceptionally high yields and a high impurity removal. However, the effect of varying process parameters, on the precipitation success and its limitations to SXC, is not yet well understood. For this reason, the precipitation behavior and SXC adaptation for ORFV were investigated for the PEG/ORFV contact time, the membranes pore size, and the type and concentration of ions. All three parameters influenced the ORFV recoveries significantly. A small pore size and a long contact time induced filtration effects and inhibited a full virus recovery. The application of salts had complex concentration-dependent effects on precipitation and SXC yields, and ranged from a complete prevention of precipitation in the presence of kosmotropic substances to increased efficiencies with Mg
Language	English
Publishing date	2022-10-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2614641-1
ISSN	2077-0375
ISSN	2077-0375
DOI	10.3390/membranes12111070
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Thesis ; Online: Orf Virusvektor

Amann, Ralf

Entwicklung neuer Selektionsstrategien, Identifikation neuer Fremdgen-Insertionsorte sowie Herstellung, Charakterisierung und Wirksamkeit eines rekombinanten Tollwutimpfstoffs

2014

Abstract: Virale Vektoren stellen eine vielversprechende Plattformtechnologie zur Herstellung rekombinanter Impfstoffe dar, die neben der klassischen Prävention von Infektionskrankheiten zunehmend auch zur Entwicklung neuer, innovativer Therapiekonzepte, wie ... ...

Abstract	Virale Vektoren stellen eine vielversprechende Plattformtechnologie zur Herstellung rekombinanter Impfstoffe dar, die neben der klassischen Prävention von Infektionskrankheiten zunehmend auch zur Entwicklung neuer, innovativer Therapiekonzepte, wie beispielsweise als therapeutische Tumorimpfstoffe, eingesetzt werden. Die vorgelegte Dissertation basiert auf Arbeiten mit dem Orf-Virusvektor D1701-V und lässt sich in zwei Schwerpunktthemen, rekombinante Impfstoffentwicklung und Vektormodifikation, untergliedern. So konnten erfolgreich Impfstoffe gegen Tollwut und Influenza A Viruserkrankungen hergestellt und die Wirksamkeit im Tiermodell erfolgreich untersucht werden. Zusätzlich konnten Grundlagen zur Generierung polyvalenter Orf Virusvektoren konzipiert und eine neue, hocheffiziente Selektionsmethode entwickelt werden.
Keywords	ddc:570
Language	German
Publishing date	2014-05-16
Publishing country	de
Document type	Thesis ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Thesis ; Online: Orf Virusvektor

Amann, Ralf

Entwicklung neuer Selektionsstrategien, Identifikation neuer Fremdgen-Insertionsorte sowie Herstellung, Charakterisierung und Wirksamkeit eines rekombinanten Tollwutimpfstoffs

2014

Abstract	Virale Vektoren stellen eine vielversprechende Plattformtechnologie zur Herstellung rekombinanter Impfstoffe dar, die neben der klassischen Prävention von Infektionskrankheiten zunehmend auch zur Entwicklung neuer, innovativer Therapiekonzepte, wie beispielsweise als therapeutische Tumorimpfstoffe, eingesetzt werden. Die vorgelegte Dissertation basiert auf Arbeiten mit dem Orf-Virusvektor D1701-V und lässt sich in zwei Schwerpunktthemen, rekombinante Impfstoffentwicklung und Vektormodifikation, untergliedern. So konnten erfolgreich Impfstoffe gegen Tollwut und Influenza A Viruserkrankungen hergestellt und die Wirksamkeit im Tiermodell erfolgreich untersucht werden. Zusätzlich konnten Grundlagen zur Generierung polyvalenter Orf Virusvektoren konzipiert und eine neue, hocheffiziente Selektionsmethode entwickelt werden.
Keywords	Text ; ddc:570
Language	German
Publishing date	2014-05-16
Publisher	o. Verl.
Publishing country	de
Document type	Thesis ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: Stability studies for the identification of critical process parameters for a pharmaceutical production of the Orf virus.

Eilts, Friederike / Labisch, Jennifer J / Orbay, Sabri / Harsy, Yasmina M J / Steger, Marleen / Pagallies, Felix / Amann, Ralf / Pflanz, Karl / Wolff, Michael W

Vaccine

2023 Volume 41, Issue 32, Page(s) 4731–4742

Abstract: A promising new vaccine platform is based on the Orf virus, a viral vector of the genus Parapoxvirus, which is currently being tested in phase I clinical trials. The application as a vaccine platform mandates a well-characterised, robust, and efficient ... ...

Abstract	A promising new vaccine platform is based on the Orf virus, a viral vector of the genus Parapoxvirus, which is currently being tested in phase I clinical trials. The application as a vaccine platform mandates a well-characterised, robust, and efficient production process. To identify critical process parameters in the production process affecting the virus' infectivity, the Orf virus was subjected to forced degradation studies, including thermal, pH, chemical, and mechanical stress conditions. The tests indicated a robust virus infectivity within a pH range of 5-7.4 and in the presence of the tested buffering substances (TRIS, HEPES, PBS). The ionic strength up to 0.5 M had no influence on the Orf virus' infectivity stability for NaCl and MgCl
MeSH term(s)	Humans ; Orf virus/genetics ; Freezing ; Genetic Vectors ; Pharmaceutical Preparations
Chemical Substances	Pharmaceutical Preparations
Language	English
Publishing date	2023-06-21
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2023.06.047
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1930: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 458: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Comparison of sample preparation techniques for the physicochemical characterization of Orf virus particles

Eilts, Friederike / Steger, Marleen / Pagallies, Felix / Rziha, Hanns-Joachim / Hardt, Martin / Amann, Ralf / Wolff, Michael W.

Journal of virological methods. 2022 Dec., v. 310

2022

Abstract: The determination of the electrostatic charge of biological nanoparticles requires a purified, mono-disperse, and concentrated sample. Previous studies proofed an impact of the preparation protocol on the stability and electro-hydrodynamics of viruses, ... ...

Abstract	The determination of the electrostatic charge of biological nanoparticles requires a purified, mono-disperse, and concentrated sample. Previous studies proofed an impact of the preparation protocol on the stability and electro-hydrodynamics of viruses, whereas commonly used methods are often complex and do not allow the required sample throughput. In the present study, the application of the (I) steric exclusion chromatography (SXC) for the Orf virus (ORFV) purification and subsequent physicochemical characterization was evaluated and compared to (II) SXC followed by centrifugal diafiltration and (III) sucrose cushion ultracentrifugation. The three methods were characterized in terms of protein removal, size distribution, infectious virus recovery, visual appearance, and electrophoretic mobility as a function of pH. All preparation techniques achieved a protein removal of more than 99 %, and (I) an infectious ORFV recovery of more than 85 %. Monodisperse samples were realized by (I) and (III). In summary, ORFV samples prepared by (I) and (III) displayed comparable quality. Additionally, (I) offered the shortest operation time and easy application. Based on the obtained data, the three procedures were ranked according to eight criteria of possible practical relevance, which delineate the potential of SXC as virus preparation method for physicochemical analysis.
Keywords	Orf virus ; electrophoresis ; electrostatic interactions ; gel chromatography ; nanoparticles ; pH ; sucrose ; ultracentrifugation ; viruses
Language	English
Dates of publication	2022-12
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	8013-5
ISSN	1879-0984 ; 0166-0934
ISSN (online)	1879-0984
ISSN	0166-0934
DOI	10.1016/j.jviromet.2022.114614
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 1565: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Stability studies for the identification of critical process parameters for a pharmaceutical production of the Orf virus

Eilts, Friederike / Labisch, Jennifer J. / Orbay, Sabri / Harsy, Yasmina M.J. / Steger, Marleen / Pagallies, Felix / Amann, Ralf / Pflanz, Karl / Wolff, Michael W.

Vaccine. 2023 July, v. 41, no. 32 p.4731-4742

2023

Abstract	A promising new vaccine platform is based on the Orf virus, a viral vector of the genus Parapoxvirus, which is currently being tested in phase I clinical trials. The application as a vaccine platform mandates a well-characterised, robust, and efficient production process. To identify critical process parameters in the production process affecting the virus’ infectivity, the Orf virus was subjected to forced degradation studies, including thermal, pH, chemical, and mechanical stress conditions. The tests indicated a robust virus infectivity within a pH range of 5–7.4 and in the presence of the tested buffering substances (TRIS, HEPES, PBS). The ionic strength up to 0.5 M had no influence on the Orf virus’ infectivity stability for NaCl and MgCl₂, while NH₄Cl destabilized significantly. Furthermore, short-term thermal stress of 2d up to 37 °C and repeated freeze-thaw cycles (20cycles) did not affect the virus’ infectivity. The addition of recombinant human serum albumin was found to reduce virus inactivation. Last, the Orf virus showed a low shear sensitivity induced by peristaltic pumps and mixing, but was sensitive to ultrasonication. The isoelectric point of the applied Orf virus genotype D1707-V was determined at pH3.5. The broad picture of the Orf virus’ infectivity stability against environmental parameters is an important contribution for the identification of critical process parameters for the production process, and supports the development of a stable pharmaceutical formulation. The work is specifically relevant for enveloped (large DNA) viruses, like the Orf virus and like most vectored vaccine approaches.
Keywords	DNA ; Orf virus ; drug formulations ; freeze-thaw cycles ; genotype ; human serum albumin ; ionic strength ; isoelectric point ; mechanical stress ; pH ; pathogenicity ; thermal stress ; ultrasonic treatment ; vaccines ; viruses ; CPB ; DLS ; DMEM ; DSP ; FCS ; GFP ; IU ; MVA ; ORFV ; PBS ; PCTRL ; pI ; rHSA ; Downstream processing ; Forced degradation studies ; Formulation ; Parapox Orf virus ; Viral vector vaccine
Language	English
Dates of publication	2023-07
Size	p. 4731-4742.
Publishing place	Elsevier Ltd
Document type	Article ; Online
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2023.06.047
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 1930: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 458: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito