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  1. Article ; Online: The Differences in Bioactivity between Crude and Purified Cholera Toxin from Local Isolate of Vibrio cholera

    Amina N. Al- Thwani1

    The Iraqi Journal of Veterinary Medicine, Vol 36, Iss 0E (2012)

    2012  

    Abstract: In this investigate Cholera toxin was purified from the local clinical isolate designated as V. cholerae S . Few steps were employed for purification of CT including concentration of the protein, back extraction, and gel filtration. The bioactivity of CT ...

    Abstract In this investigate Cholera toxin was purified from the local clinical isolate designated as V. cholerae S . Few steps were employed for purification of CT including concentration of the protein, back extraction, and gel filtration. The bioactivity of CT reflected that crude and purified toxin had lethal activity on Balb/c mice in which a dose of 2.5µg/ ml of purified toxin caused mice death with symptoms such as muscle cramps and tachycardia while erythematous and induration was seen at intradermal I. d injection of skin of Guinea pig. Histopathological examination for liver, intestine, adrenal gland and spleen of white mice showed that liver tissue was more affected compared with spleen, intestine and adrenal gland tissues.
    Keywords Veterinary medicine ; SF600-1100
    Language English
    Publishing date 2012-04-01T00:00:00Z
    Publisher University of Baghdad, College of Veterinary Medicine
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Detection similarity and differences between Uropathogenic Escherichia coli isolated from recurrent urinary tract infections and bladder cancer patients

    Jafar Jumaa AL-Kuriashy / .Amina N. Al-Thwani / Samer fadhel AL-wazni

    مجله كليه طب الكندي, Vol 12, Iss

    2016  Volume 1

    Abstract: Background: Urinary tract infections (UTIs) and their complications such as Bladder cancer (Bl. C.) are a health growing problem worldwide. Objective: To shed light on this subject, present study was done to investigate relationship between recurrent ... ...

    Abstract Background: Urinary tract infections (UTIs) and their complications such as Bladder cancer (Bl. C.) are a health growing problem worldwide. Objective: To shed light on this subject, present study was done to investigate relationship between recurrent urinary tract infection (RUTI) due to Escherichia coli (E. coli) and Bl. C.Type of study: Cross-sectional study. Methods: This study included 130 patients with RUTI, 50 patients with Bl. C. and 50 control of both sexes (aged 7-85 years) attending Al-Zahra Teaching Hospital in Al-Kut/Wassit governorate and Al-Harery Teaching Hospital of specialized surgeries/Baghdad. The patients were divided into two groups: the first group (n=130) included those who were suffering from recurrent UTI without bladder cancer and diagnosed clinically as having recurrent UTI. The second group(n=50) included those who had bladder cancer. One hundred and thirty morning midstream urine specimens were collected from recurrent urinary tract infection patients and 50 from healthy persons as a control and also 50 biopsy specimens collected from recurrent UTI with bladder cancer(after surgical operation to these patients) during beginning of October 2012 to end of March 2013. Results: Intracellular bacterial communities (ICBC) (namely Escherichia coli) was isolated from (68/130) 53% from patients with RUTI while (12/50) 24% isolated from patients with Bladder cancer In this study, other molecular technique called Repetitive extragenic palindromic (REP) were used for drawing the genetic map of bacteria to know the points of similarity and differences between isolated bacteria. A difference between bacteria in each group were found, but when comparing the genetic map of UPEC isolated from patients with Bl. C. with those isolated from patients with recurrent UTI high difference between them were seen. Conclusion: Detecting the intracellular bacterial communities (namely E. coli) in patients with recurrent UTI, with or without bladder cancer. Detecting similarity and difference in genetic map of ...
    Keywords Urinary tract infection ; Bladder cancer ; Uropathogenic Escherichia coli ; Medicine ; R
    Subject code 616 ; 610
    Language English
    Publishing date 2016-06-01T00:00:00Z
    Publisher University of Baghdad/ Al-Kindy College of Medicine
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Molecular Epidemiology and Genotyping of Mycobacterium tuberculosis Isolated in Baghdad

    Ruqaya Mustafa Ali / Alberto Trovato / David Couvin / Amina N. Al-Thwani / Emanuele Borroni / Fahim H. Dhaer / Nalin Rastogi / Daniela M. Cirillo

    BioMed Research International, Vol

    2014  Volume 2014

    Abstract: Tuberculosis (TB) remains a major health problem in Iraq but the strains responsible for the epidemic have been poorly characterized. Our aim was to characterize the TB strains circulating in Bagdad (Iraq). A total of 270 Mycobacterium tuberculosis ... ...

    Abstract Tuberculosis (TB) remains a major health problem in Iraq but the strains responsible for the epidemic have been poorly characterized. Our aim was to characterize the TB strains circulating in Bagdad (Iraq). A total of 270 Mycobacterium tuberculosis complex (MTBC) strains isolated between 2010 and 2011 from TB patients attending the Center of Chest and Respiratory diseases in Baghdad were analyzed by Spoligotyping. The analysis indicated that 94.1% of the isolates belong to known genotype clades: CAS 39.6%, ill-defined T clade 29.6%, Manu 7.4%, Haarlem 7%, Ural 4.1%, LAM 3.3%, X 0.7%, LAM7-TUR 0.7%, EAI 0.7%, S 0.7%, and unknown 5.9%. Comparison with the international multimarker database SITVIT2 showed that SIT 309 (CAS1-Delhi) and SIT1144 (T1) were the most common types. In addition, 44 strains were included in SITVIT2 database under 16 new Spoligotype International Types (SITs); of these, 6 SITs (SIT3346, SIT3497, SIT3708, SIT3790, SIT3791, and SIT3800) (n = 32 strains) were created within the present study and 10 were created after a match with an orphan in the database. By using 24-loci MIRU-VNTR-typing on a subset of 110 samples we found a high recent transmission index (RTI) of 33.6%. In conclusion, we present the first unifying framework for both epidemiology and evolutionary analysis of M. tuberculosis in Iraq.
    Keywords Medicine ; R
    Subject code 630
    Language English
    Publishing date 2014-01-01T00:00:00Z
    Publisher Hindawi Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    Inter-library loan at ZB MED

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