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Article ; Online: Identification of Knockdown Resistance Mutations in the Cimex hemipterus (Hemiptera: Cimicidae) in Iran.

Tiotour, Mehdi / Shaddel, Minoo / Aminianfar, Mohammad / Mirahmadi, Hadi / Barzegar, Gholamreza / Solgi, Rahmat / Darvishi, Mohammad

The American journal of tropical medicine and hygiene

2022 Volume 107, Issue 1, Page(s) 204–207

Abstract: The worldwide resurgence of tropical bed bug Cimex hemipterus beginning in the late 1990s has led to growing concern. Molecular data on pyrethroid resistance, which is essential for the control strategies, is unknown for C. hemipterus in Iran. The ... ...

Abstract	The worldwide resurgence of tropical bed bug Cimex hemipterus beginning in the late 1990s has led to growing concern. Molecular data on pyrethroid resistance, which is essential for the control strategies, is unknown for C. hemipterus in Iran. The current study evaluated the deltamethrin resistance status of C. hemipterus by bioassay and molecular tests. Live bed bugs were collected from sleeping quarters (dormitories) in the city of Tehran and used for insecticide bioassay tests. For bioassay evaluation, mixed-sex pools of adult bugs were exposed to deltamethrin (0.025%)-treated paper. Polymerase chain reaction assay evaluated resistance-related mutations in the voltage-gated sodium channel gene (VGSC) gene of studied populations. On the basis of the bioassay test within the 48-h exposure to deltamethrin, C. hemipterus were determined to be resistant. Knockdown time ratios (KR50) in the studied populations of C. hemipterus was 5.5-fold compared with those of the C. lectularius Teh strain. DNA sequencing of the VGSC gene revealed the presence of mutations at M918I and L1014 in C. hemipterus. According to the bioassay and molecular results of current study, C. hemipterus showed a high degree of pyrethroid resistance. The application of multiple approaches including physical, biological, and chemical tests should be regarded in future bed bug control strategies.
MeSH term(s)	Animals ; Bedbugs/drug effects ; Bedbugs/genetics ; Insecticide Resistance/genetics ; Insecticides ; Iran ; Mutation ; Pyrethrins/pharmacology
Chemical Substances	Insecticides ; Pyrethrins
Language	English
Publishing date	2022-06-06
Publishing country	United States
Document type	Journal Article
ZDB-ID	2942-7
ISSN	1476-1645 ; 0002-9637
ISSN (online)	1476-1645
ISSN	0002-9637
DOI	10.4269/ajtmh.22-0092
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Article ; Online: In vitro and in vivo Assessment of Silver Nanoparticles Against Clostridium botulinum Type A Botulinum.

Aminianfar, Mohammad / Parvardeh, Siavash / Soleimani, Mohsen

Current drug discovery technologies

2018 Volume 16, Issue 1, Page(s) 113–119

Abstract: Background: Clostridium botulinum causes botulism, a serious paralytic illness that results from the ingestion of a botulinum toxin. Because silver nanoparticle products exhibit strong antimicrobial activity, applications for silver nanoparticles in ... ...

Abstract	Background: Clostridium botulinum causes botulism, a serious paralytic illness that results from the ingestion of a botulinum toxin. Because silver nanoparticle products exhibit strong antimicrobial activity, applications for silver nanoparticles in healthcare have expanded. Therefore, the objective of the current study was to assess a therapeutic strategy for the treatment of botulism toxicity using silver nanoparticles. Methods: A preliminary test was conducted using doses that produce illness in laboratory animals to determine the absolute lethal dose (LD100) of botulinum toxin type A (BoNT/A) in mice. Next, the test animals were divided into six groups containing six mice each. Groups I, II and III were the negative control (botulinum toxin only), positive control-1 (nano-silver only) and positive control-2 (no treatment), respectively. The remaining groups were allocated to the toxin that was supplemented with three nano-silver treatments. Results: The mortality rates of mice caused by BoNT/A significantly reduced in the treatment groups with different doses and injection intervals of nano-silver when compared to the negative control group. BoNT/A toxicity induced by intraperitoneal injection of the toxin of Clostridium botulinum causes rapid death while when coupled with nano-osilver results in delayed death in mice. Conclusion: These results, while open to future improvement, represent a preliminary step towards the satisfactory control of BoNT/A with the use of silver nanoparticles for human protection against this bioterrorism threat. Further study in this area can elucidate the underlying mechanism for detoxifying BoNT/A by silver nanoparticles.
MeSH term(s)	Animals ; Botulinum Toxins, Type A/toxicity ; Botulism/drug therapy ; Clostridium botulinum type A ; Metal Nanoparticles/administration & dosage ; Mice ; Silver/administration & dosage
Chemical Substances	Silver (3M4G523W1G) ; Botulinum Toxins, Type A (EC 3.4.24.69)
Language	English
Publishing date	2018-04-03
Publishing country	United Arab Emirates
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1875-6220
ISSN (online)	1875-6220
DOI	10.2174/1570163815666180403163946
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Article: The Accuracy of Emergency Physicians in Ultrasonographic Screening of Acute Appendicitis; a Cross Sectional Study.

Karimi, Ebrahim / Aminianfar, Mohammad / Zarafshani, Keivan / Safaie, Arash

Emergency (Tehran, Iran)

2017 Volume 5, Issue 1, Page(s) e22

Abstract: Introduction: Diagnostic values reported for ultrasonographic screening of acute appendicitis vary widely and are dependent on the operator's skill, patient's gender, weight, etc. The present study aimed to evaluate the effect of operator skill on the ... ...

Abstract	Introduction: Diagnostic values reported for ultrasonographic screening of acute appendicitis vary widely and are dependent on the operator's skill, patient's gender, weight, etc. The present study aimed to evaluate the effect of operator skill on the diagnostic accuracy of ultrasonography in detection of appendicitis by comparing the results of ultrasonography done by radiologists and emergency physicians. Methods: This prospective diagnostic accuracy was carried out on patients suspected to acute appendicitis presenting to EDs of 2 hospitals. After the initial clinical examinations, all the patients underwent ultrasonography for appendicitis by emergency physician and radiologist, respectively. The final diagnosis of appendicitis was based on either pathology report or 48-hour follow-up. Screening performance characteristics of appendix ultrasonography by emergency physician and radiologist were compared using STATA 11.0 software. Results: 108 patients with the mean age of 23.91 ± 7.46 years were studied (61.1% male). Appendicitis was confirmed for 37 (34.26%) cases. Cohen's kappa coefficient between ultrasonography by the radiologist and emergency physician in diagnosis of acute appendicitis was 0.51 (95% CI: 0.35 - 0.76). Area under the ROC curve of ultrasonography in appendicitis diagnosis was 0.78 (95% CI: 0.69 - 0.86) for emergency physician and 0.88 (95% CI: 0.81 - 0.94) for radiologist (p = 0.052). Sensitivity and specificity of ultrasonography by radiologist and emergency physician in appendicitis diagnosis were 83.87% (95% CI: 67.32 - 93.23), 91.5% (95% CI: 81.89 - 96.52), 72.97% (95% CI: 55.61 - 85.63), and 83.10% (95% CI: 71.94 - 90.59), respectively. Conclusion: Findings of the present study showed that the diagnostic accuracy of ultrasonography carried out by radiologist (89%) is a little better compared to that of emergency physician (80%) in diagnosis of appendicitis, but none are excellent.
Language	English
Publishing date	2017-01-10
Publishing country	Iran
Document type	Journal Article
ZDB-ID	2754468-0
ISSN	2345-4571 ; 2345-4563
ISSN (online)	2345-4571
ISSN	2345-4563
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Article: Evaluation of hepatitis C, hepatitis B, and HIV virus Serology pandemic in thalassemia patients of Shahid Mohammadi Hospital of Bandar Abbas, Iran.

Aminianfar, Mohammad / Khani, Farrokh / Ghasemzadeh, Iman

Electronic physician

2017 Volume 9, Issue 3, Page(s) 4014–4019

Abstract: Introduction: Thalassemia patients are prone to the hepatitis C, B, and HIV virus, due to their constant need to receive blood transfusions. Therefore, this research was aimed to determine the epidemic of the aforementioned diseases in thalassemia ... ...

Abstract	Introduction: Thalassemia patients are prone to the hepatitis C, B, and HIV virus, due to their constant need to receive blood transfusions. Therefore, this research was aimed to determine the epidemic of the aforementioned diseases in thalassemia patients of Shahid Mohammadi Hospital of Bandar Abbas, Iran. Methods: This cross-sectional study was carried out on thalassemia patients visiting the Thalassemia Hospital of Bandar Abbas from March 21, 2014, to March 22, 2015. Checklists were used to collect the data. HBS-Ag, HCV-Ab, and HIV-Ab tests have been registered for hepatitis B, hepatitis C, and HIV virus, respectively. The data were analyzed using SPSS 19 by Mann-Whitney U test, chi-square test, and independent samples t-test. Results: Among 587 records administered into the research, 280 individuals (47.7 %) were men and (52.3 %) were female. The average age of participants was 18.01 ± 9.31 years. The youngest was 1 years old, and the oldest was 46 years old. Four patients under the study (0.7 %) were positive for HBV; 60 (10.2 %) were HCV positive. However, no one in the study was diagnosed HIV positive. In the case of spread, there was no significant difference regarding age and sex in HBV patients. However, in HCV patients, significant differences were found (p=0.044 for gender and p=0.001 for age). Conclusion: In thalassemia, hepatitis C had the highest rate of spread. According to the screening, it was much anticipated that the trend should be falling, but the results show the contrary. Hence, it is recommended that more precise methods such as PCR be used.
Language	English
Publishing date	2017-03-25
Publishing country	Iran
Document type	Journal Article
ZDB-ID	2524187-4
ISSN	2008-5842
ISSN	2008-5842
DOI	10.19082/4014
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Article ; Online: COVID-19 and its implications on the clinico-radiological course of multiple sclerosis: A case-control study.

Rahmani, Mohammad / Moghadasi, Abdorreza Naser / Shahi, Shayan / Eskandarieh, Sharareh / Azizi, Hossein / Hasanzadeh, Alireza / Ahmadzade, Ali / Dehnavi, Ali Zare / Farahani, Ramin Hamidi / Aminianfar, Mohammad / Naeini, Alireza Ranjbar

Medicina clinica (English ed.)

2023 Volume 160, Issue 5, Page(s) 187–192

Abstract: Background: Multiple sclerosis (MS) is an immune-mediated disease that has been related to several risk factors such as various viral infections. We carried out this study in order to establish a relationship between COVID-19 infection and MS severity.!# ...

Abstract	Background: Multiple sclerosis (MS) is an immune-mediated disease that has been related to several risk factors such as various viral infections. We carried out this study in order to establish a relationship between COVID-19 infection and MS severity. Methods: In a case-control study, we recruited patients with relapsing-remitting multiple sclerosis (RRMS). Patients were divided into two groups based on positive COVID-19 PCR at the end of the enrollment phase. Each patient was prospectively followed for 12 months. Demographical, clinical, and past medical history were collected during routine clinical practice. Assessments were performed every six months; MRI was performed at enrollment and 12 months later. Results: Three hundred and sixty-two patients participated in this study. MS patients with COVID-19 infection had significantly higher increases in the number of MRI lesions ( Conclusion: COVID-19 may lead to higher disability scores in the RRMS population and is associated with developing new Gd-enhancing lesions in MRI imaging. However, no difference was observed between the groups regarding the number of relapses during follow-up.
Language	English
Publishing date	2023-03-03
Publishing country	Spain
Document type	Journal Article
ISSN	2387-0206
ISSN (online)	2387-0206
DOI	10.1016/j.medcle.2022.06.021
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Article: A multi-method and structure-based in silico vaccine designing against

Pourseif, Mohammad Mostafa / Yousefpour, Mitra / Aminianfar, Mohammad / Moghaddam, Gholamali / Nematollahi, Ahmad

BioImpacts : BI

2019 Volume 9, Issue 3, Page(s) 131–144

Abstract: Introduction: ...

Abstract	Introduction:
Language	English
Publishing date	2019-03-08
Publishing country	Iran
Document type	Journal Article
ZDB-ID	2604624-6
ISSN	2228-5660 ; 2228-5652
ISSN (online)	2228-5660
ISSN	2228-5652
DOI	10.15171/bi.2019.18
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Article: Efficacy of a standardized herbal formulation from

Soleiman-Meigooni, Saeed / Hoseini Yekta, Nafiseh / Sheikhan, Hamid Reza / Aminianfar, Mohammad / Hamidi-Farahani, Ramin / Ahmadi, Mousa / Asgari, Ali / Kazemi-Galougahi, Mohammad Hasan / Rahimi, Roja

Journal of Ayurveda and integrative medicine

2022 Volume 13, Issue 4, Page(s) 100670

Abstract: Introduction: As no specific pharmacological intervention has been known for COVID-19, medicinal plants may be a suitable candidate for management of this disease. The aim of this study was to investigate the efficacy of a herbal syrup from licorice as ... ...

Abstract	Introduction: As no specific pharmacological intervention has been known for COVID-19, medicinal plants may be a suitable candidate for management of this disease. The aim of this study was to investigate the efficacy of a herbal syrup from licorice as an adjuvant treatment in hospitalized patients with COVID-19. Materials and methods: 213 hospitalized patients diagnosed with COVID-19 were assigned to receive either standardized licorice syrup as an adjuvant treatment plus standard care [Syrup Group (SYRUP), N = 91], or standard care alone [Standard Group (STANDARD), N = 104], for 7 days. The primary endpoint was duration of hospitalization in survivors. The secondary endpoints included 25% increase in oxygen saturation, C-reactive protein (CRP) difference and lymphocyte difference from baseline, number of death and number of patients transferred to ICU. Results: Mean duration of admission was 5.24 days in SYRUP and 7.14 days in STANDARD (p < 0.001). Oxygen saturation increased in 86 of 91 patients (94.5%) in the licorice group, compared to 83 of 104 patients (79.8%) in the control group (p = 0.002). There was no significant difference between the two groups in the number of patients died during hospitalization (p = 0.837). Five patients in SYRUP and 16 patients in STANDARD were transferred to ICU (p < 0.026). Mean reduction in CRP (p < 0.001) and mean increase in the number of lymphocytes (p = 0.008) in SYRUP were significantly higher than STANDARD. Discussion: Licorice syrup as an adjuvant treatment demonstrated promising results on duration of hospital admission, O2 saturation as well as inflammatory markers in COVID-19 patients; however, further clinical studies with larger sample size are suggested to achieve more conclusive results.
Language	English
Publishing date	2022-10-28
Publishing country	United States
Document type	Journal Article
ISSN	0975-9476
ISSN	0975-9476
DOI	10.1016/j.jaim.2022.100670
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Article ; Online: COVID-19 and its implications on the clinico-radiological course of multiple sclerosis: A case-control study.

Medicina clinica

2022 Volume 160, Issue 5, Page(s) 187–192

Abstract	Background: Multiple sclerosis (MS) is an immune-mediated disease that has been related to several risk factors such as various viral infections. We carried out this study in order to establish a relationship between COVID-19 infection and MS severity. Methods: In a case-control study, we recruited patients with relapsing-remitting multiple sclerosis (RRMS). Patients were divided into two groups based on positive COVID-19 PCR at the end of the enrollment phase. Each patient was prospectively followed for 12 months. Demographical, clinical, and past medical history were collected during routine clinical practice. Assessments were performed every six months; MRI was performed at enrollment and 12 months later. Results: Three hundred and sixty-two patients participated in this study. MS patients with COVID-19 infection had significantly higher increases in the number of MRI lesions (p: 0.019, OR(CI): 6.37(1.54-26.34)) and EDSS scores (p: 0.017), but no difference was found in total annual relapses or relapse rates. COVID-19 infections were positively correlated with EDSS progression (p: 0.02) and the number of new MRI lesions (p: 0.004) and predicted the likelihood of the number of new MRI lesions by an odds of 5.92 (p: 0.018). Conclusion: COVID-19 may lead to higher disability scores in the RRMS population and is associated with developing new Gd-enhancing lesions in MRI imaging. However, no difference was observed between the groups regarding the number of relapses during follow-up.
MeSH term(s)	Humans ; Multiple Sclerosis/diagnostic imaging ; Case-Control Studies ; COVID-19/diagnostic imaging ; Multiple Sclerosis, Relapsing-Remitting/diagnostic imaging ; Magnetic Resonance Imaging ; Recurrence ; Disease Progression
Language	Spanish
Publishing date	2022-08-10
Publishing country	Spain
Document type	Journal Article
ZDB-ID	411607-0
ISSN	1578-8989 ; 0025-7753
ISSN (online)	1578-8989
ISSN	0025-7753
DOI	10.1016/j.medcli.2022.06.020
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Article: Efficacy of Red Blood Cell Exchange as Adjunctive Treatment for Hypoxemia and Survival Rate of Patients With Severe Coronavirus-2 Disease: An Open-Labeled Phase 2 Randomized Clinical Trial.

Aminianfar, Mohammad / Soleiman-Meigooni, Saeed / Hamidi-Farahani, Ramin / Darvishi, Mohammad / Hoseini-Shokouh, Seyyed Javad / Asgari, Ali / Faraji-Hormozi, Syrous / Asli, Maryam

Frontiers in medicine

2022 Volume 9, Page(s) 899593

Abstract: Background: Severe acute respiratory syndrome (SARS) coronavirus-2 may infect red blood cells (RBCs) and impact oxygenation. We aimed to evaluate the efficacy of RBC exchange as an adjunctive treatment for hypoxemia and the survival rate of patients ... ...

Abstract	Background: Severe acute respiratory syndrome (SARS) coronavirus-2 may infect red blood cells (RBCs) and impact oxygenation. We aimed to evaluate the efficacy of RBC exchange as an adjunctive treatment for hypoxemia and the survival rate of patients with severe coronavirus disease 2019 (COVID-19). Methods: In a randomized clinical trial, we divided sixty patients with severe COVID-19 into two groups. The intervention group received the standard treatment of severe COVID-19 with RBC exchange three to four times in 2 days. The control group only received the standard treatment. Our primary outcomes were improving hypoxemia in 7 days, recovery or discharge, and death in 28 days. We conducted Chi-square test, independent samples Results: Twenty-nine men and thirty-one women with a mean age of 67.5 years entered the study. The frequency of hypertension and diabetes mellitus was 86.7 and 68.3%, respectively. The most common symptoms of severe COVID-19 were dyspnea (91.6%), cough (75%), and fever (66.6%). Our results showed that hypoxemia improved in 21 of the 30 patients (70%) in the intervention group and 10 of the 30 patients (33.3%) in the control group ( Conclusion: The RBC exchange improved the oxygenation and survival rate in patients with severe COVID-19.
Language	English
Publishing date	2022-07-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2775999-4
ISSN	2296-858X
ISSN	2296-858X
DOI	10.3389/fmed.2022.899593
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Article: Antibiotic resistance in patients suffering from nosocomial infections in Besat Hospital.

Hormozi, Sirous Faraji / Vasei, Narges / Aminianfar, Mohammad / Darvishi, Mohammad / Saeedi, Ali Asghar

European journal of translational myology

2018 Volume 28, Issue 3, Page(s) 7594

Abstract: This study was performed to determine the trend of antibiotic resistance of the causative organisms among the patients suffering from nosocomial infections in Besat Hospital since 2013 to 2015. In this observational study that was performed as a ... ...

Abstract	This study was performed to determine the trend of antibiotic resistance of the causative organisms among the patients suffering from nosocomial infections in Besat Hospital since 2013 to 2015. In this observational study that was performed as a retrospective cohort, 935 consecutive patients with nosocomial infection were enrolled in Besat Hospital since 2013 to 2015. The trend of antibiotic resistance of the causative organisms among them was determined and contributing factors were assessed. The finding of this study revealed that type of microorganisms had significant variation (p = 0.024): while the gram-negative bacilli have shown an increased level of resistance, the gram positive cocci had less resistance. The antibiotic resistance was increased for ampicillin/sulbactam, piperacillin/tazoactam, ceftriaxone, ceftazidime, cefepime, meropenem, gentamicin, amikacin, ciprofloxacine, levofloxacine, nitrofurantoin, and ampicilline. However, it was decreased for colistin. In conclusion, antibiotic resistance has an increasing trend and strategic measures of prevention are needed to reduce nosocomial infections.
Language	English
Publishing date	2018-07-16
Publishing country	Italy
Document type	Journal Article
ZDB-ID	2545577-1
ISSN	2037-7460 ; 2037-7452
ISSN (online)	2037-7460
ISSN	2037-7452
DOI	10.4081/ejtm.2018.7594
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