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Article ; Online: In vitro and in vivo anti-cancer effects of hibernating common carp (Cyprinus carpio) plasma on metastatic triple-negative breast cancer

Mojtaba Golpich / Elham Amini / Amirhosein Kefayat / Mehrafarin Fesharaki / Jamal Moshtaghian

Scientific Reports, Vol 12, Iss 1, Pp 1-

2022 Volume 15

Abstract: Abstract Uncontrollable proliferation is a hallmark of cancer cells. Cell proliferation and migration are significantly depressed during hibernation state. Many studies believe some factors in the plasma of hibernating animals cause these effects. This ... ...

Abstract	Abstract Uncontrollable proliferation is a hallmark of cancer cells. Cell proliferation and migration are significantly depressed during hibernation state. Many studies believe some factors in the plasma of hibernating animals cause these effects. This study aimed to assess the anti-cancer effects of hibernating common carp (Cyprinus carpio) plasma on 4T1 cancer cells in vitro and in vivo. The effect of hibernating plasma on cell viability, morphology, migration, apoptosis rate, and cell cycle distribution of 4T1 cells was investigated in vitro and in vivo. Hibernating plasma at a concentration of 16 mg/ml significantly reduced the viability of 4T1 cancer cells, without any toxicity on L929 normal fibroblast cells. It could change the morphology of cancer cells, induced apoptosis and cell cycle arrest at the G2/M phase, and inhibited migration. Furthermore, intratumoral injection of hibernating plasma (200 µl, 16 mg/ml) in the tumor-bearing mice caused a significant inhibition of 4T1 breast tumors volume (46.9%) and weight (58.8%) compared with controls. A significant decrease in the number of metastatic colonies at the lungs (80%) and liver (52.8%) of hibernating plasma-treated animals was detected which increased the survival time (21.9%) compared to the control groups. Immunohistochemical analysis revealed a considerable reduction in the Ki-67-positive cells in the tumor section of the hibernating plasma-treated animals compared with controls. Taken together, the SDS-PAGE and mass spectrometry analysis indicated the alpha-2-macroglobulin level in the hibernating fish plasma was significantly increased. It could exert an anti-cancer effect on breast cancer cells and suggested as a novel cancer treatment strategy.
Keywords	Medicine ; R ; Science ; Q
Subject code	610
Language	English
Publishing date	2022-02-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Evaluation of radiation and ammonium lactate effects on hyaluronic acid expression as a pro-cancerous factor in supernatant and exosome isolated from supernatant of primary mouse fibroblast cell culture

Nasrin Zare / Amirhosein Kefayat / Shaghayegh Haghjooy Javanmard

International Journal of Preventive Medicine, Vol 11, Iss 1, Pp 125-

2020 Volume 125

Abstract: Background: Previous studies show that aberrant synthesis of Hyaluronan accelerates tumor growth, angiogenesis, and metastasis. The fibroblasts are probably responsible for most of the hyaluronic acid (HA) accumulation in tumor microenvironment after ... ...

Abstract	Background: Previous studies show that aberrant synthesis of Hyaluronan accelerates tumor growth, angiogenesis, and metastasis. The fibroblasts are probably responsible for most of the hyaluronic acid (HA) accumulation in tumor microenvironment after radiotherapy. Our goal is to investigate and compare radiation and lactate effects on HA levels in supernatant and exosome isolated from supernatant of primary mouse fibroblast cell culture. Methods: Fibroblast cells were prepared from skin of C57BL6 mouse. These cells were divided into three groups (no treatment, cells treated with 10 mM ammonium lactate, and irradiated cells). Then supernatant was harvested from FBS-free culture media after 48 h. Exosomes were purified by differential centrifugation (300 × g for 10 min, 2000 × g for 30 min, 16500 g for 30 min) and were pelleted by ultracentrifugation (150,000 × g for 180 min). Size of exosomes was determined using a Zetasizer. HA concentration measured using a HA ELISA Kit. Data were analyzed using one-way ANOVA. Results: There was a significant increase in HA-coated exosomes isolated from supernatants of irradiated cells compared to untreated cell and cells treated with 10 mM ammonium lactate (P < 0.001). As well, there was a significant increase in the HA concentration in the supernatants of cells treated with 10 mM ammonium lactate relative to untreated cells and irradiated cells (P < 0.05). Conclusions: It seems that routine radiation therapy leads to massive shedding of HA-coated exosomes by normal fibroblast cells and thus exosomes-HA may contribute to tumor promotion and induce of the premetastatic niche.
Keywords	exosomes ; hyaluronic acid ; radiation ; Medicine ; R
Subject code	571
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Wolters Kluwer Medknow Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Highly improved pH-Responsive anticancer drug delivery and T2-Weighted MRI imaging by magnetic MOF CuBTC-based nano/microcomposite

Zahra Gharehdaghi / Seyed Morteza Naghib / Rahmatollah Rahimi / Atin Bakhshi / Amirhosein Kefayat / Armin shamaeizadeh / Fatemeh Molaabasi

Frontiers in Molecular Biosciences, Vol

2023 Volume 10

Abstract: Cu-BTC framework has received a considerable attention in recent years as a drug carrier candidate for cancer treatment due to its unique structural properties and promising biocompatibility. However, its intrinsic deficiency for medical imaging ... ...

Abstract	Cu-BTC framework has received a considerable attention in recent years as a drug carrier candidate for cancer treatment due to its unique structural properties and promising biocompatibility. However, its intrinsic deficiency for medical imaging potentially limits its bioapplications; To address this subject, a magnetic nano/microscale MOF has been successfully fabricated by introducing Fe3O4 nanoparticles as an imaging agent into the porous isoreticular MOF [Cu3(BTC)2] as a drug carrier. The synthesized magnetic MOFs exhibits a high loading capacity (40.5%) toward the model anticancer DOX with an excellent pH-responsive drug release. The proposed nanocomposite not only possesses large surface area, high magnetic response, large mesopore volume, high transverse relaxivity (r2) and good stability but also exhibits superior biocompatibility, specific tumor cellular uptake, and significant cancer cell viability inhibitory effect without any targeting agent. It is expected that the synthesized magnetic nano/microcomposite may be used for clinical purposes and can also serve as a platform for photoactive antibacterial therapy ae well as pH/GSH/photo-triple-responsive nanocarrier.
Keywords	magnetic ; metal-organic framework ; controlled release ; pH-responsive doxorubicin release ; magnetic resonance imaging ; multifunctional nanocomposite ; Biology (General) ; QH301-705.5
Subject code	500
Language	English
Publishing date	2023-04-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: C-phycocyanin

Amirhosein Kefayat / Fatemeh Ghahremani / Ashkan Safavi / Alireza Hajiaghababa / Jamal Moshtaghian

Scientific Reports, Vol 9, Iss 1, Pp 1-

a natural product with radiosensitizing property for enhancement of colon cancer radiation therapy efficacy through inhibition of COX-2 expression

2019 Volume 13

Abstract: Abstract Different chemical and nanomaterial agents have been introduced for radiosensitizing purposes. However, many researchers believe these agents are far away from clinical application due to side effects and limited knowledge about their behavior ... ...

Abstract	Abstract Different chemical and nanomaterial agents have been introduced for radiosensitizing purposes. However, many researchers believe these agents are far away from clinical application due to side effects and limited knowledge about their behavior in the human body. In this study, C-phycocyanin (C-PC) was used as a natural radiosensitizer for enhancement of radiation therapy (RT) efficacy. C-PC treatment’s effect on the COX-2 expression of cancer cells was investigated by flow cytometry, western blot, qRT-PCR analyses in vitro and in vivo. Subsequently, the radiosensitizing effect of C-PC treatment was investigated by MTT and clonogenic cell survival assays for CT-26, DLD-1, HT-29 colon cancer cell lines and the CRL-1831 as normal colonic cells. In addition, the C-PC treatment effect on the radiation therapy efficacy was evaluated according to CT-26 tumor’s growth progression and immunohistochemistry analyses of Ki-67 labeling index. C-PC treatment (200 µg/mL) could significantly enhance the radiation therapy efficacy in vitro and in vivo. Synergistic interaction was detected at C-PC and radiation beams co-treatment based on Chou and Talalay formula (combination index <1), especially at 200 µg/mL C-PC and 6 Gy radiation dosages. The acquired DEF of C-PC treatment was 1.39, 1.4, 1.63, and 1.05 for CT-26, DLD-1, HT-29, and CRL-1831 cells, respectively. Also, C-PC + RT treated mice exhibited 35.2% lower mean tumors’ volume and about 6 days more survival time in comparison with the RT group (P < 0.05). In addition, C-PC + RT group exhibited 54% lower Ki-67 index in comparison with the RT group. Therefore, C-PC can exhibit high radiosensitizing effects. However, the potential cardiovascular risks of C-PC as a COX-2 inhibitor should be evaluated with extensive preclinical testing before developing this agent for clinical trials.
Keywords	Medicine ; R ; Science ; Q
Subject code	616
Language	English
Publishing date	2019-12-01T00:00:00Z
Publisher	Nature Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: ارزیابی ترمیم زخم و خواص ضد میکروبی پانسمان زخم هیدروژلی نشاسته‌ی حاوی عصاره‌ی اتانولی پروپولیس در موش صحرایی

Asghar Eskandarinia / Mohamamd Rafienia / Mosayeb Gharakhloo / Sepehr Navid / Amirhosein Kefayat

مجله دانشکده پزشکی اصفهان, Vol 35, Iss 458, Pp 1744-

2018 Volume 1748

Abstract: مقدمه: هدف از انجام تحقیق حاضر این بود که بتوان با ترکیب نشاسته و پروپولیس به روش ریخته‏گری، نوعی پانسمان هیدروژلی تهیه کرد که از نظر ویژگی‏ها، برای درمان زخم مناسب باشد. پانسمان‌های موجود، توانایی محافظت مطلوبی را از صدمات و آلودگی‌های ایجاد شده به ... ...

Abstract	مقدمه: هدف از انجام تحقیق حاضر این بود که بتوان با ترکیب نشاسته و پروپولیس به روش ریخته‏گری، نوعی پانسمان هیدروژلی تهیه کرد که از نظر ویژگی‏ها، برای درمان زخم مناسب باشد. پانسمان‌های موجود، توانایی محافظت مطلوبی را از صدمات و آلودگی‌های ایجاد شده به وسیله‏ی میکرواورگانیسم‌ها ندارد. روش‌ها: برای ساخت فیلم، نسبت‏ مشخصی از نشاسته‌ی ذرت با آب دو بار تقطیر مخلوط و سپس گلیسرول با نسبت 100/40 وزن خشک نشاسته به آن افزوده شد. عصاره‌ی اتانولی پروپولیس تهیه شده با نسبت‌های 25/0، 5/0 و 1 درصد به محلول اضافه و در یک قالب تفلونی، ریخته‏گری گردید و ساختار و ویژگی آن با استفاده از خواص ضد میکروبی، ميكروسكوپ الكتروني پويشي (Scanning electron microscope یا SEM) و آزمون حیوانی مورد بررسی قرار گرفت. یافته‌ها: خواص ضد میکروبی پانسمان زخم با افزایش غلظت پروپولیس، افزایش یافت. طبق یافته‌های به دست آمده، اختلاف معنی‌داری در اندازه‌ی زخم گروه پانسمان شونده با هیدروژل نشاسته‌ی حاوی عصاره‌ی اتانولی پروپولیس و گروه شاهد وجود داشت (05/0 > P). نتیجه‌گیری: استفاده از پانسمان هیدروژلی نشاسته‌ی ذرت حاوی عصاره‌ی اتانولی پروپولیس، مي‌تواند در كنترل عفونت زخم‌هاي سطحي مؤثر باشد.
Keywords	Starch ; Propolis ; Wound dressing ; Medicine ; R ; Medicine (General) ; R5-920
Language	Persian
Publishing date	2018-02-01T00:00:00Z
Publisher	Vesnu Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Immunoprotective effect of an in silico designed multiepitope cancer vaccine with BORIS cancer-testis antigen target in a murine mammary carcinoma model

Elham Mahdevar / Amirhosein Kefayat / Ashkan Safavi / Amirhossein Behnia / Seyed Hossein Hejazi / Amaneh Javid / Fatemeh Ghahremani

Scientific Reports, Vol 11, Iss 1, Pp 1-

2021 Volume 13

Abstract: Abstract In our previous study, immunoinformatic tools were used to design a novel multiepitope cancer vaccine based on the most immunodominant regions of BORIS cancer-testis antigen. The final vaccine construct was an immunogenic, non-allergenic, and ... ...

Abstract	Abstract In our previous study, immunoinformatic tools were used to design a novel multiepitope cancer vaccine based on the most immunodominant regions of BORIS cancer-testis antigen. The final vaccine construct was an immunogenic, non-allergenic, and stable protein consisted of multiple cytotoxic T lymphocytes epitopes, IFN-γ inducing epitopes, and B cell epitopes according to bioinformatic analyzes. Herein, the DNA sequence of the final vaccine construct was placed into the pcDNA3.1 vector as a DNA vaccine (pcDNA3.1-VAC). Also, the recombinant multiepitope peptide vaccine (MPV) was produced by a transfected BL21 E. coli strain using a recombinant pET-28a vector and then, purified and screened by Fast protein liquid chromatography technique (FPLC) and Western blot, respectively. The anti-tumor effects of prophylactic co-immunization with these DNA and protein cancer vaccines were evaluated in the metastatic non-immunogenic 4T1 mammary carcinoma in BALB/c mice. Co-immunization with the pcDNA3.1-VAC and MPV significantly (P < 0.001) increased the serum levels of the MPV-specific IgG total, IgG2a, and IgG1. The splenocytes of co-immunized mice exhibited a significantly higher efficacy to produce interleukin-4 and interferon-γ and proliferation in response to MPV in comparison with the control. The prophylactic co-immunization regime caused significant breast tumors’ growth inhibition, tumors’ weight decrease, inhibition of metastasis formation, and enlarging tumor-bearing mice survival time, without any considerable side effects. Taking together, this cancer vaccine can evoke strong immune response against breast tumor and inhibits its growth and metastasis.
Keywords	Medicine ; R ; Science ; Q
Subject code	616
Language	English
Publishing date	2021-11-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Polyurethane-Nanolignin Composite Foam Coated with Propolis as a Platform for Wound Dressing

Zari Pahlevanneshan / Mohammadreza Deypour / Amirhosein Kefayat / Mohammad Rafienia / Paweł Sajkiewicz / Rasoul Esmaeely Neisiany / Mohammad Saeid Enayati

Polymers, Vol 13, Iss 3191, p

Synthesis and Characterization

2021 Volume 3191

Abstract: This piece of research explores porous nanocomposite polyurethane (PU) foam synthesis, containing nanolignin (NL), coated with natural antimicrobial propolis for wound dressing. PU foam was synthesized using polyethylene glycol, glycerol, NL, and 1, 6- ... ...

Abstract	This piece of research explores porous nanocomposite polyurethane (PU) foam synthesis, containing nanolignin (NL), coated with natural antimicrobial propolis for wound dressing. PU foam was synthesized using polyethylene glycol, glycerol, NL, and 1, 6-diisocyanato-hexane (NCO/OH ratio: 1.2) and water as blowing agent. The resultant foam was immersed in ethanolic extract of propolis (EEP). PU, NL-PU, and PU-NL/EEP foams were characterized from mechanical, morphological, and chemical perspectives. NL Incorporation into PU increased mechanical strength, while EEP coating showed lower strength than PU-NL/EEP. Morphological investigations confirmed an open-celled structure with a pore diameter of 150–200 μm, a density of nearly 0.2 g/cm 3, , and porosity greater than 85%, which led to significantly high water absorption (267% for PU-NL/EEP). The hydrophilic nature of foams, measured by the contact angle, proved to be increased by NL addition and EEP coating. PU and PU-NL did not show important antibacterial features, while EEP coating resulted in a significant antibacterial efficiency. All foams revealed high biocompatibility toward L929 fibroblasts, with the highest cell viability and cell attachment for PU-NL/EEP. In vivo wound healing using Wistar rats’ full-thickness skin wound model confirmed that PU-NL/EEP exhibited an essentially higher wound healing efficacy compared with other foams. Hence, PU-NL/EEP foam could be a promising wound dressing candidate.
Keywords	polyurethane foam ; nanolignin ; propolis ; wound dressing ; Organic chemistry ; QD241-441
Subject code	600
Language	English
Publishing date	2021-09-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Identifying the most effective hydatid cyst fluid fraction for anticancer vaccination of 4T1 breast tumor-bearing mice

Shima Daneshpour / Shahla Rostamirad / Amirhosein Kefayat / MohammadReza Mofid / Ashkan Safavi / Hossein Yousofi Darani

International Journal of Preventive Medicine, Vol 10, Iss 1, Pp 143-

2019 Volume 143

Abstract: Background: The hydatid cyst fluid antigens have high homology with cancer cell antigens and also exhibit considerable immunogenicity. Therefore, their utilization for cancer immunization can cause an effective antitumor immune response. However, the ... ...

Abstract	Background: The hydatid cyst fluid antigens have high homology with cancer cell antigens and also exhibit considerable immunogenicity. Therefore, their utilization for cancer immunization can cause an effective antitumor immune response. However, the main challenge is identifying the most effective antigens for this purpose. Methods: Hydatid cyst fluid fractions including the glycolipid fraction, glycoprotein fraction, 78 kDa fraction, and antigen B fraction were prepared. Then, the BALB/c mice were immunized against different antigens and, subsequently, 4T1 cells were subcutaneously implanted. The tumors' growth, metastasis, and tumor-bearing mice survival were assessed in different immunized groups. In addition, IL-2, IL-4, IFN-γ, and TNF-α serum levels were estimated to evaluate the immune system response. Results: BALB/c mice immunization against the complete hydatid cyst fluid antigens exhibited more significant inhibition of the tumors' growth and metastasis and increase of tumor-bearing mice survival in comparison with its derived fractions. However, the 78 kDa fraction exhibited the best results according to the same factors in comparison with all the prepared fractions. Conclusions: The 78 kDa fraction of the hydatid cyst fluid was the most effective fraction of hydatid cyst fluid for immunization against 4T1 breast tumors.
Keywords	breast neoplasm ; cyst fluid ; immunization ; immunotherapy ; neoplasm metastasis ; Medicine ; R
Subject code	616
Language	English
Publishing date	2019-01-01T00:00:00Z
Publisher	Wolters Kluwer Medknow Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: In vitro and in vivo performance of a propolis-coated polyurethane wound dressing with high porosity and antibacterial efficacy

Khodabakhshi, Darioush / Asghar Eskandarinia / Amirhosein Kefayat / Mohammad Rafienia / Sepehr Navid / Saeed Karbasi / Jamal Moshtaghian

Colloids and surfaces. 2019 June 01, v. 178

2019

Abstract: Wound dressings with antimicrobial and wound healing accelerating properties are emerging as valuable options to prevent wound infection and improve the wound healing process. In this study, high porous polyurethane (PU) foams were successfully prepared ... ...

Abstract	Wound dressings with antimicrobial and wound healing accelerating properties are emerging as valuable options to prevent wound infection and improve the wound healing process. In this study, high porous polyurethane (PU) foams were successfully prepared using salt leaching/solvent casting method and were coated with propolis as a well-known anti-bacterial agent. The wound dressings were subjected to detail analyzes using electron microscopy, reflectance Fourier transform infrared spectroscopy, mechanical properties, contact angle measurement, ratio swelling, porosity measurement, and in vitro and in vivo evaluations. The prepared wound dressings had high porosity (more than 80%) with homogeneous pore structure and sufficient interconnectivity. The increase of propolis concentration (10%–30%) caused tensile strength decrease (5.26 ± 0.40–2.99 ± 0.11 MPa), elongation at break increase (372 ± 12–434 ± 22%), contact angle decrease (114.52 ± 2.31° to 35.53 ± 1.65°), water absorption decreased (243 ± 15–207 ± 14%) and enhancement of the antibacterial activity against Escherichia coli and Staphylococcus aureus. The propolis coated wound dressing exhibited significant enhancement of in vitro cellular compatibility and in vivo wound healing which had direct relative with coated propolis concentration. Therefore, propolis-coated polyurethane wound dressing can be an appropriate candidate for more pre-clinical investigations.
Keywords	Escherichia coli ; Fourier transform infrared spectroscopy ; Staphylococcus aureus ; antibacterial properties ; antibiotics ; contact angle ; electron microscopy ; foams ; in vivo studies ; leaching ; polyurethanes ; porosity ; propolis ; reflectance ; solvents ; tensile strength ; tissue repair ; water uptake
Language	English
Dates of publication	2019-0601
Size	p. 177-184.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1500523-9
ISSN	1873-4367 ; 0927-7765
ISSN (online)	1873-4367
ISSN	0927-7765
DOI	10.1016/j.colsurfb.2019.03.010
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: A Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold

Asghar Eskandarinia / Amirhosein Kefayat / Maria Agheb / Mohammad Rafienia / Moloud Amini Baghbadorani / Sepehr Navid / Karim Ebrahimpour / Darioush Khodabakhshi / Fatemeh Ghahremani

Scientific Reports, Vol 10, Iss 1, Pp 1-

2020 Volume 15

Abstract: Abstract One-layer wound dressings cannot meet all the clinical needs due to their individual characteristics and shortcomings. Therefore, bilayer wound dressings which are composed of two layers with different properties have gained lots of attention. ... ...

Abstract	Abstract One-layer wound dressings cannot meet all the clinical needs due to their individual characteristics and shortcomings. Therefore, bilayer wound dressings which are composed of two layers with different properties have gained lots of attention. In the present study, polycaprolactone/gelatin (PCL/Gel) scaffold was electrospun on a dense membrane composed of polyurethane and ethanolic extract of propolis (PU/EEP). The PU/EEP membrane was used as the top layer to protect the wound area from external contamination and dehydration, while the PCL/Gel scaffold was used as the sublayer to facilitate cells’ adhesion and proliferation. The bilayer wound dressing was investigated regarding its microstructure, mechanical properties, surface wettability, anti-bacterial activity, biodegradability, biocompatibility, and its efficacy in the animal wound model and histopathological analyzes. Scanning electron micrographs exhibited uniform morphology and bead-free structure of the PCL/Gel scaffold with average fibers’ diameter of 237.3 ± 65.1 nm. Significant anti-bacterial activity was observed against Staphylococcal aureus (5.4 ± 0.3 mm), Escherichia coli (1.9 ± 0.4 mm) and Staphylococcus epidermidis (1.0 ± 0.2 mm) according to inhibition zone test. The bilayer wound dressing exhibited high hydrophilicity (51.1 ± 4.9°), biodegradability, and biocompatibility. The bilayer wound dressing could significantly accelerate the wound closure and collagen deposition in the Wistar rats’ skin wound model. Taking together, the PU/EEP-PCL/Gel bilayer wound dressing can be a potential candidate for biomedical applications due to remarkable mechanical properties, biocompatibility, antibacterial features, and wound healing activities.
Keywords	Medicine ; R ; Science ; Q
Subject code	620
Language	English
Publishing date	2020-02-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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