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  1. Article: The major promoter of the rat insulin-like growth factor-I gene binds a protein complex that is required for basal expression.

    An, M R / Lowe, W L

    Molecular and cellular endocrinology

    1995  Volume 114, Issue 1-2, Page(s) 77–89

    Abstract: IGF-I gene transcription is regulated by two promoters--the major promoter which is active in all tissues and regulates transcription of IGF-I mRNAs that contain exon 1 and a second promoter which regulates transcription of IGF-I mRNAs that contain exon ... ...

    Abstract IGF-I gene transcription is regulated by two promoters--the major promoter which is active in all tissues and regulates transcription of IGF-I mRNAs that contain exon 1 and a second promoter which regulates transcription of IGF-I mRNAs that contain exon 2 and from which significant transcription is restricted to the liver. The major promoter is a TATAA-less promoter that lacks both a CAAT box and SP1 binding sites and that utilizes multiple transcription initiation sites. The current studies were designed to delineate the functional elements of the major promoter. Transient transfection assays using rat C6 glioma cells and rat dermal fibroblasts in primary culture demonstrated that basal activity of the major promoter was located between -18 (with +1 defined as the most 5' transcription initiation site in exon 1) and +78 of exon 1. DNase I footprinting, which was performed using nuclear extracts from rat C6 glioma cells, demonstrated protein binding to a sequence that extended from -10 to +9 (termed IGFI-FP1). In gel shift assays, binding of C6 cell nuclear proteins to a 34-basepair (bp) oligonucleotide that contained IGFI-FP1 resulted in the formation of three specific protein-DNA complexes. The functional role of protein binding to IGFI-FP1 was examined by mutating the sequences between either -4 and -2 or -9 and -7 in IGF-I-luciferase fusion genes that contained either 412 or 18 bp of 5'-flanking region and 302 bp of exon 1. Both of these mutations altered protein binding to IGFI-FP1 as demonstrated by gel shift analysis. Transfection of the wild-type and mutant fusion genes into C6 glioma cells demonstrated that mutation of the nucleotides between -4 and -2 decreased luciferase activity to approximately 50% of wild-type activity, whereas mutation of the nucleotides between -9 and -7 decreased luciferase activity to 11-35% of wild-type activity. These data demonstrate that: (i) basal activity of the major promoter of the rat IGF-I gene is localized to the region between -18 and +78 of exon 1; (ii) protein binding sites are present within this region of the major promoter; and (iii) protein binding to this region contributes to basal expression of the IGF-I gene.
    MeSH term(s) Animals ; Base Sequence ; Cell Line ; Cloning, Molecular ; DNA/genetics ; DNA/metabolism ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Gene Expression ; Insulin-Like Growth Factor I/genetics ; Luciferases/genetics ; Luciferases/metabolism ; Molecular Sequence Data ; Oligonucleotide Probes/genetics ; Promoter Regions, Genetic ; Rats ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/metabolism ; Transfection
    Chemical Substances DNA-Binding Proteins ; Oligonucleotide Probes ; Recombinant Fusion Proteins ; Insulin-Like Growth Factor I (67763-96-6) ; DNA (9007-49-2) ; Luciferases (EC 1.13.12.-)
    Language English
    Publishing date 1995-10-30
    Publishing country Ireland
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 187438-x
    ISSN 1872-8057 ; 0303-7207
    ISSN (online) 1872-8057
    ISSN 0303-7207
    DOI 10.1016/0303-7207(95)03644-m
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  2. Article: [A epidemiological survey of bladder carcinoma in chemical dye industry (author's transl)].

    An, M R

    Zhonghua wai ke za zhi [Chinese journal of surgery

    1980  Volume 18, Issue 6, Page(s) 491–493

    MeSH term(s) Adolescent ; Adult ; China ; Coloring Agents/toxicity ; Female ; Humans ; Male ; Middle Aged ; Occupational Diseases/epidemiology ; Urinary Bladder Neoplasms/chemically induced ; Urinary Bladder Neoplasms/epidemiology
    Chemical Substances Coloring Agents
    Language Chinese
    Publishing date 1980-12
    Publishing country China
    Document type Journal Article
    ZDB-ID 604573-x
    ISSN 0529-5815
    ISSN 0529-5815
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  3. Article: Developmental regulation of insulin-like growth factor-I and growth hormone receptor gene expression.

    Shoba, L / An, M R / Frank, S J / Lowe, W L

    Molecular and cellular endocrinology

    1999  Volume 152, Issue 1-2, Page(s) 125–136

    Abstract: During development, the insulin-like growth factor I (IGF-I) gene is expressed in a tissue specific manner; however, the molecular mechanisms governing its developmental regulation remain poorly defined. To examine the hypothesis that expression of the ... ...

    Abstract During development, the insulin-like growth factor I (IGF-I) gene is expressed in a tissue specific manner; however, the molecular mechanisms governing its developmental regulation remain poorly defined. To examine the hypothesis that expression of the growth hormone (GH) receptor accounts, in part, for the tissue specific expression of the IGF-I gene during development, the developmental regulation of IGF-I and GH receptor gene expression in rat tissues was examined. The level of IGF-I and GH receptor mRNA was quantified in RNA prepared from rats between day 17 of gestation (E17) and 17 months of age (17M) using an RNase protection assay. Developmental regulation of IGF-I gene expression was tissue specific with four different patterns of expression seen. In liver, IGF-I mRNA levels increased markedly between E17 and postnatal day 45 (P45) and declined thereafter. In contrast, in brain, skeletal muscle and testis, IGF-I mRNA levels decreased between P5 and 4M but were relatively unchanged thereafter. In heart and kidney, a small increase in IGF-I mRNA levels was observed between the early postnatal period and 4 months, whereas in lung, minimal changes were observed during development. The changes in GH receptor mRNA levels were, in general, coordinate with the changes in IGF-I mRNA levels, except in skeletal muscle. Interestingly, quantification of GH receptor levels by Western blot analysis in skeletal muscle demonstrated changes coordinate with IGF-I mRNA levels. The levels of the proteins which mediate GH receptor signaling (STAT1, -3, and -5, and JAK2) were quantified by Western blot analysis. These proteins also are expressed in a tissue specific manner during development. In some cases, the pattern of expression was coordinate with IGF-I gene expression, whereas in others it was discordant. To further define molecular mechanisms for the developmental regulation of IGF-I gene expression, protein binding to IGFI-FP1, a protein binding site that is in the major promoter of the rat IGF-I gene and is important for basal promoter activity in vitro, was examined. Gel shift analyses using a 34-base pair oligonucleotide that contained IGFI-FP1 did not demonstrate changes in protein binding that paralleled those in IGF-I gene expression, suggesting that protein binding to IGFI-FP1 does not contribute to the developmental regulation of IGF-I gene expression, at least in brain and liver. In summary, the present studies demonstrate coordinate expression of the IGF-I gene and GH receptor during development and suggest that GH receptor expression contributes to the tissue specific expression of the IGF-I gene during development.
    MeSH term(s) Animals ; Blotting, Western ; Embryonic and Fetal Development/genetics ; Gene Expression Regulation, Developmental ; Insulin-Like Growth Factor I/genetics ; Organ Specificity ; Promoter Regions, Genetic ; RNA, Messenger/analysis ; RNA, Messenger/genetics ; Rats ; Rats, Sprague-Dawley ; Receptors, Somatotropin/genetics
    Chemical Substances RNA, Messenger ; Receptors, Somatotropin ; Insulin-Like Growth Factor I (67763-96-6)
    Language English
    Publishing date 1999-06-25
    Publishing country Ireland
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 187438-x
    ISSN 1872-8057 ; 0303-7207
    ISSN (online) 1872-8057
    ISSN 0303-7207
    DOI 10.1016/s0303-7207(99)00045-3
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  4. Book ; Online: Study of $e^+e^-\to\gamma\phi J/\psi$ from $\sqrt{s}=4.600$ to $4.951$ GeV

    BESIII Collaboration / Ablikim, M. / Achasov, M. N. / Adlarson, P. / Albrecht, M. / Aliberti, R. / Amoroso, A. / An, M. R. / An, Q. / Bai, Y. / Bakina, O. / Ferroli, R. Baldini / Balossino, I. / Ban, Y. / Batozskaya, V. / Becker, D. / Begzsuren, K. / Berger, N. / Bertani, M. /
    Bettoni, D. / Bianchi, F. / Bianco, E. / Bloms, J. / Bortone, A. / Boyko, I. / Briere, R. A. / Brueggemann, A. / Cai, H. / Cai, X. / Calcaterra, A. / Cao, G. F. / Cao, N. / Cetin, S. A. / Chang, J. F. / Chang, W. L. / Che, G. R. / Chelkov, G. / Chen, C. / Chen, Chao / Chen, G. / Chen, H. S. / Chen, M. L. / Chen, S. J. / Chen, S. M. / Chen, T. / Chen, X. R. / Chen, X. T. / Chen, Y. B. / Chen, Z. J. / Cheng, W. S.

    2022  

    Abstract: Using data samples with an integrated luminosity of $6.4$~fb$^{-1}$ collected by the BESIII detector operating at the BEPCII storage ring, the process of $e^+e^-\to\gamma\phi J/\psi$ is studied. The processes of $e^+e^-\to\phi\chi_{c1,c2}$, $\chi_{c1,c2}\ ...

    Abstract Using data samples with an integrated luminosity of $6.4$~fb$^{-1}$ collected by the BESIII detector operating at the BEPCII storage ring, the process of $e^+e^-\to\gamma\phi J/\psi$ is studied. The processes of $e^+e^-\to\phi\chi_{c1,c2}$, $\chi_{c1,c2}\to\gamma J/\psi$ are observed with a significance of more than $10\sigma$. The $\sqrt{s}$-dependent cross section of $e^+e^- \to \phi\chi_{c1,c2}$ is measured between 4.600 and 4.951~GeV, and evidence of a resonance structure is found for the first time in the $\phi\chi_{c2}$ process. We also search for the processes of $e^+e^-\to\gamma X(4140)$, $\gamma X(4274)$ and $\gamma X(4500)$ via the $\gamma\phi J/\psi$ final state, but no obvious structures are found. The upper limits on the production cross section times the branching fraction for these processes at the 90% confidence level are reported.

    Comment: 35 pages, 9 figures
    Keywords High Energy Physics - Experiment
    Subject code 612
    Publishing date 2022-10-24
    Publishing country us
    Document type Book ; Online
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  5. Book ; Online: First Direct Measurement of the Absolute Branching Fraction of $\Sigma^+ \to \Lambda e^+ \nu_{e}$

    BESIII Collaboration / Ablikim, M. / Achasov, M. N. / Adlarson, P. / Aliberti, R. / Amoroso, A. / An, M. R. / An, Q. / Bai, Y. / Bakina, O. / Ferroli, R. Baldini / Balossino, I. / Ban, Y. / Batozskaya, V. / Becker, D. / Begzsuren, K. / Berger, N. / Bertani, M. / Bettoni, D. /
    Bianchi, F. / Bianco, E. / Bloms, J. / Bortone, A. / Boyko, I. / Briere, R. A. / Brueggemann, A. / Cai, H. / Cai, X. / Calcaterra, A. / Cao, G. F. / Cao, N. / Cetin, S. A. / Chang, J. F. / Chang, T. T. / Chang, W. L. / Che, G. R. / Chelkov, G. / Chen, C. / Chen, Chao / Chen, G. / Chen, H. S. / Chen, M. L. / Chen, S. J. / Chen, S. M. / Chen, T. / Chen, X. R. / Chen, X. T. / Chen, Y. B. / Chen, Y. Q. / Chen, Z. J.

    2022  

    Abstract: The first direct measurement of the absolute branching fraction of $\Sigma^+ \to \Lambda e^+ \nu_{e}$ is reported based on an $e^+e^-$ annihilation sample of $(10087\pm44) \times 10^6$ $J/\psi$ events collected with the BESIII detector at $\sqrt{s}=3.097$ ...

    Abstract The first direct measurement of the absolute branching fraction of $\Sigma^+ \to \Lambda e^+ \nu_{e}$ is reported based on an $e^+e^-$ annihilation sample of $(10087\pm44) \times 10^6$ $J/\psi$ events collected with the BESIII detector at $\sqrt{s}=3.097$ GeV. The branching fraction is determined to be ${\mathcal B}(\Sigma^+ \to \Lambda e^+ \nu_{e}) = [2.93\pm0.74(\rm stat) \pm 0.13(\rm syst)]\times 10^{-5}$, which is the most precise measurement obtained in a single experiment to date and also the first result obtained at a collider experiment. Combining this result with the world average of ${\mathcal B}(\Sigma^- \to \Lambda e^- \bar{\nu}_{e})$ and the lifetimes of $\Sigma^{\pm}$, the ratio, $\frac{\Gamma(\Sigma^- \to \Lambda e^- \bar{\nu}_{e})}{\Gamma(\Sigma^+ \to \Lambda e^+ \nu_{e})}$, is determined to be $1.06 \pm 0.28$, which is within 1.8 standard deviations of the value expected in the absence of second-class currents that are forbidden in the Standard Model.

    Comment: 8 pages, 3 figures
    Keywords High Energy Physics - Experiment ; High Energy Physics - Phenomenology
    Subject code 612
    Publishing date 2022-12-10
    Publishing country us
    Document type Book ; Online
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  6. Book ; Online: First Observation of the Semileptonic Decay $\Lambda_c^+\rightarrow pK^- e^+\nu_e$

    Ablikim, M. / Achasov, M. N. / Adlarson, P. / Albrecht, M. / Aliberti, R. / Amoroso, A. / An, M. R. / An, Q. / Bai, X. H. / Bai, Y. / Bakina, O. / Ferroli, R. Baldini / Balossino, I. / Ban, Y. / Batozskaya, V. / Becker, D. / Begzsuren, K. / Berger, N. / Bertani, M. /
    Bettoni, D. / Bianchi, F. / Bloms, J. / Bortone, A. / Boyko, I. / Briere, R. A. / Brueggemann, A. / Cai, H. / Cai, X. / Calcaterra, A. / Cao, G. F. / Cao, N. / Cetin, S. A. / Chang, J. F. / Chang, W. L. / Chelkov, G. / Chen, C. / Chen, Chao / Chen, G. / Chen, H. S. / Chen, M. L. / Chen, S. J. / Chen, S. M. / Chen, T. / Chen, X. R. / Chen, X. T. / Chen, Y. B. / Chen, Z. J. / Cheng, W. S. / Choi, S. K. / Chu, X.

    2022  

    Abstract: Using $4.5 \mathrm{fb}^{-1}$ of $e^+e^-$ annihilation data samples collected at the center-of-mass energies ranging from 4.600 GeV to 4.699 GeV with the BESIII detector at the BEPCII collider, a first study of $\Lambda_c^+$ decaying semileptonically into ...

    Abstract Using $4.5 \mathrm{fb}^{-1}$ of $e^+e^-$ annihilation data samples collected at the center-of-mass energies ranging from 4.600 GeV to 4.699 GeV with the BESIII detector at the BEPCII collider, a first study of $\Lambda_c^+$ decaying semileptonically into the inclusive $pK^-$ system $\Lambda_c^+\rightarrow pK^-e^+\nu_e$ is performed. The $\Lambda_c^+\rightarrow pK^-e^+\nu_e$ decay is observed with a significance of $8.2\sigma$ and the branching fraction is measured to be $\mathcal{B}(\Lambda_c^+\rightarrow pK^- e^+\nu_e)=(0.82\pm0.15_{\rm stat.}\pm0.06_{\rm syst.})\times 10^{-3}$. In addition, first evidence for the decay $\Lambda_c^+\rightarrow \Lambda(1520) e^+\nu_e$ is obtained with a significance of $3.3\sigma$ and the branching fraction is measured to be $\mathcal{B}(\Lambda_c^+\rightarrow \Lambda(1520) e^+\nu_e)=(1.36\pm0.56_{\rm stat.}\pm0.14_{\rm syst.})\times 10^{-3}$. We also measure the branching fraction for $\Lambda_c^+\rightarrow pK^-_{\rm non-\Lambda(1520)} e^+\nu_e$ to be $\mathcal{B}(\Lambda_c^+\rightarrow pK^-_{\rm non-\Lambda(1520)} e^+\nu_e)=(0.53\pm0.15_{\rm stat.}\pm0.06_{\rm syst.})\times 10^{-3}$. Using a measurement of the inclusive semileptonic $\Lambda_c^+$ branching fraction from BESIII, the relative branching fractions are determined to be $[\mathcal{B}(\Lambda_c^+\rightarrow pK^-e^+\nu_e)/\mathcal{B}(\Lambda_c^+\rightarrow X e^+\nu_e)]=(2.1\pm0.4_{\rm stat.}\pm0.1_{\rm syst.})\%$ and $[\mathcal{B}(\Lambda_c^+\rightarrow \Lambda^*(1520) e^+\nu_e)/\mathcal{B}(\Lambda_c^+\rightarrow X e^+\nu_e)]=(3.4\pm1.4_{\rm stat.}\pm0.4_{\rm syst.})\%$. These measurements provide a clear confirmation that semileptonic $\Lambda_c^+$ decays are not saturated by the $\Lambda \ell^+\nu_{\ell}$ final state.

    Comment: 13 pages, 9 figures and 2 tables
    Keywords High Energy Physics - Experiment
    Subject code 612
    Publishing date 2022-07-23
    Publishing country us
    Document type Book ; Online
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  7. Book ; Online: Amplitude analysis and branching-fraction measurement of $D_{s}^{+} \to \pi^{+}\pi^{0}\eta^{\prime}$

    BESIII Collaboration / Ablikim, M. / Achasov, M. N. / Adlarson, P. / Albrecht, M. / Aliberti, R. / Amoroso, A. / An, M. R. / An, Q. / Bai, X. H. / Bai, Y. / Bakina, O. / Ferroli, R. Baldini / Balossino, I. / Ban, Y. / Batozskaya, V. / Becker, D. / Begzsuren, K. / Berger, N. /
    Bertani, M. / Bettoni, D. / Bianchi, F. / Bloms, J. / Bortone, A. / Boyko, I. / Briere, R. A. / Brueggemann, A. / Cai, H. / Cai, X. / Calcaterra, A. / Cao, G. F. / Cao, N. / Cetin, S. A. / Chang, J. F. / Chang, W. L. / Chelkov, G. / Chen, C. / Chen, Chao / Chen, G. / Chen, H. S. / Chen, M. L. / Chen, S. J. / Chen, S. M. / Chen, T. / Chen, X. R. / Chen, X. T. / Chen, Y. B. / Chen, Z. J. / Cheng, W. S. / Chu, X.

    2022  

    Abstract: Using data collected with the BESIII detector in $e^+e^-$ collisions at center-of-mass energies between 4.178 and 4.226 GeV and corresponding to 6.32~fb$^{-1}$ of integrated luminosity, we report the amplitude analysis and branching-fraction measurement ... ...

    Abstract Using data collected with the BESIII detector in $e^+e^-$ collisions at center-of-mass energies between 4.178 and 4.226 GeV and corresponding to 6.32~fb$^{-1}$ of integrated luminosity, we report the amplitude analysis and branching-fraction measurement of the $D^+_s \to \pi^+ \pi^0 \eta^{\prime}$ decay. We find that the dominant intermediate process is $D^+_s \to\rho^+ \eta^{\prime}$ and the significances of other resonant and nonresonant processes are all less than $3\sigma$. The upper limits on the branching fractions of $S$-wave and $P$-wave nonresonant components are set to $0.10\%$ and $0.74\%$ at the $90\%$ confidence level, respectively. In addition, the branching fraction of the $D^+_s \to \pi^+ \pi^0 \eta^{\prime}$ decay is measured to be $(6.15\pm0.25(\rm stat.)\pm0.18(\rm syst.))\%$, which receives significant contribution only from $D_s^+\to \rho^+\eta^{\prime}$ according to the amplitude analysis.
    Keywords High Energy Physics - Experiment
    Subject code 551
    Publishing date 2022-02-08
    Publishing country us
    Document type Book ; Online
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  8. Article: Differential expression of three C/EBP isoforms in multiple tissues during the acute phase response.

    Alam, T / An, M R / Papaconstantinou, J

    The Journal of biological chemistry

    1992  Volume 267, Issue 8, Page(s) 5021–5024

    Abstract: Eucaryotic organisms possess natural defense processes triggered by stress factors such as injury, infection, and inflammation. The acute phase response is an early defense mechanism during which striking changes in protein synthesis occur in the liver ... ...

    Abstract Eucaryotic organisms possess natural defense processes triggered by stress factors such as injury, infection, and inflammation. The acute phase response is an early defense mechanism during which striking changes in protein synthesis occur in the liver and other tissues. The altered expression of many acute phase protein genes is at the transcriptional level. Some of these genes have DNA binding sites for the CCAAT/enhancer binding protein (C/EBP) family of transcription factors. We report here that in vivo expression of three isoforms of C/EBP is dramatically changed during the acute phase response. The steady-state mRNA levels of C/EBP alpha decreased significantly in the liver, lung, and fat tissues of lipopolysaccharide (LPS)-treated mice; moreover, nuclear run-off transcription assays indicated a decrease in the rate of C/EBP alpha gene transcription in isolated liver nuclei. The steady-state levels of C/EBP beta and a new isoform, C/EBP delta, were dramatically increased in many tissues within 4 h following LPS treatment. The rates of transcription of these two genes were only minimally altered in liver but significantly increased in kidney nuclei isolated from stimulated animals. Thus, the C/EBP isoforms exhibit differential mechanisms in their responses to LPS in various tissues and are likely to play an important role in mediating the transcriptional activation of genes involved in the acute phase response.
    MeSH term(s) Acute-Phase Proteins/genetics ; Animals ; Blotting, Northern ; CCAAT-Enhancer-Binding Proteins ; DNA-Binding Proteins/genetics ; Gene Expression ; Lipopolysaccharides/toxicity ; Liver/physiology ; Male ; Mice ; Mice, Inbred BALB C ; Nuclear Proteins/genetics ; Organ Specificity ; RNA/genetics ; RNA/isolation & purification ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Transcription Factors/genetics ; Transcription, Genetic
    Chemical Substances Acute-Phase Proteins ; CCAAT-Enhancer-Binding Proteins ; DNA-Binding Proteins ; Lipopolysaccharides ; Nuclear Proteins ; RNA, Messenger ; Transcription Factors ; RNA (63231-63-0)
    Language English
    Publishing date 1992-03-15
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2997-x
    ISSN 1083-351X ; 0021-9258
    ISSN (online) 1083-351X
    ISSN 0021-9258
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  9. Article: Effects of mercuric chloride on the regulation of expression of the acute phase response components alpha(1)-acid glycoprotein and C/EBP transcription factors.

    Yiangou, M / Scott, S G / Rabek, J P / An, M R / Xiong, W / Papaconstantinou, J

    Biochimica et biophysica acta

    2001  Volume 1518, Issue 1-2, Page(s) 47–56

    Abstract: We have previously shown that in response to treatment with HgCl(2), the adult mouse liver exhibits both transcriptional and translational regulation of the acute phase response genes. In this study we asked whether the heavy metal treatment affects the ... ...

    Abstract We have previously shown that in response to treatment with HgCl(2), the adult mouse liver exhibits both transcriptional and translational regulation of the acute phase response genes. In this study we asked whether the heavy metal treatment affects the regulation of the C/EBP transcription factors which play a key role in regulation of the acute phase response gene. Our studies have shown that the AGP gene is transcriptionally activated while transcription of the CCAAT/enhancer-binding trans-activating protein (C/EBP)alpha gene is slightly down-regulated and that of the C/EBPbeta gene does not respond. Both the C/EBPalpha and C/EBPbeta mRNAs produce multiple isoforms possibly by alternative translation initiation (ATI) of multiple internal AUG initiation sites. The C/EBPbeta mRNA appears to be stabilized. Although similar regulatory processes occur in response HgCl(2) vs. LPS, our data suggest that the translational processes (ATI) are differentially affected. In addition, a major difference lies in the fact that the C/EBPbeta gene is not transcriptionally activated by HgCl(2). Our data show decreased binding activity and pool levels of the C/EBPalpha isoform (p42(C/EBPalpha)) and increased binding activity and pool levels of C/EBPbeta isoform (p35(C/EBPbeta)) in response to HgCl(2). We propose that this isoform may be involved in the regulation of AGP gene expression in response to heavy metals and that there is a significant difference between the HgCl(2)-mediated and LPS-mediated inflammatory response.
    MeSH term(s) Alternative Splicing ; Animals ; CCAAT-Enhancer-Binding Protein-alpha/genetics ; CCAAT-Enhancer-Binding Protein-alpha/metabolism ; CCAAT-Enhancer-Binding Protein-beta/genetics ; CCAAT-Enhancer-Binding Protein-beta/metabolism ; COS Cells ; Cercopithecus aethiops ; DNA/metabolism ; Gene Expression Regulation ; Genetic Vectors ; Lipopolysaccharides/pharmacology ; Liver/metabolism ; Male ; Mercuric Chloride/pharmacology ; Mice ; Mice, Inbred BALB C ; Orosomucoid/genetics ; Promoter Regions, Genetic ; Protein Isoforms/genetics ; Transcription, Genetic ; Transfection
    Chemical Substances CCAAT-Enhancer-Binding Protein-alpha ; CCAAT-Enhancer-Binding Protein-beta ; Lipopolysaccharides ; Orosomucoid ; Protein Isoforms ; Mercuric Chloride (53GH7MZT1R) ; DNA (9007-49-2)
    Language English
    Publishing date 2001-03-06
    Publishing country Netherlands
    Document type Journal Article ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 60-7
    ISSN 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    ISSN (online) 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650
    ISSN 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    DOI 10.1016/s0167-4781(01)00165-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Augmented expression of cardiac atrial natriuretic peptide system in hypertensive rats.

    An, M R / Chung, Y J / Kang, D G / Nam, S C / Lee, J

    Journal of Korean medical science

    1999  Volume 14, Issue 5, Page(s) 497–501

    Abstract: The present study was aimed at investigating the regulation of atrial natriuretic peptide (ANP) system in association with either enhanced or attenuated activity of the renin-angiotensin system (RAS). The cardiac tissue mRNA and peptide levels of ANP ... ...

    Abstract The present study was aimed at investigating the regulation of atrial natriuretic peptide (ANP) system in association with either enhanced or attenuated activity of the renin-angiotensin system (RAS). The cardiac tissue mRNA and peptide levels of ANP were measured in rats with two-kidney, one clip (2K1C) or deoxycorticosterone acetate (DOCA)-salt hypertension. Plasma renin concentration was increased in 2K1C hypertension along with increases of renin mRNA and protein contents in the clipped kidney. On the contrary, it was suppressed in DOCA-salt hypertension along with decreases of renin mRNA and protein contents in the remaining kidney. The plasma ANP concentration was similarly increased in both models of hypertension. The cardiac tissue ANP contents were not significantly changed, but the tissue ANP mRNA levels were upregulated in the hypertrophied heart in these two models of hypertension. It is suggested that the cardiac ANP system is transcriptionally enhanced by cardiac hypertrophy associated with hypertension, independent of the systemic RAS.
    MeSH term(s) Animals ; Atrial Natriuretic Factor/metabolism ; Desoxycorticosterone ; Gene Expression Regulation ; Hypertension/chemically induced ; Hypertension/metabolism ; Male ; Myocardium/pathology ; Organ Size ; Peptides ; RNA, Messenger/analysis ; Rats ; Rats, Sprague-Dawley ; Renin/blood ; Renin/genetics ; Renin-Angiotensin System/physiology
    Chemical Substances Peptides ; RNA, Messenger ; Desoxycorticosterone (40GP35YQ49) ; Atrial Natriuretic Factor (85637-73-6) ; Renin (EC 3.4.23.15)
    Language English
    Publishing date 1999-10
    Publishing country Korea (South)
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 639262-3
    ISSN 1011-8934
    ISSN 1011-8934
    DOI 10.3346/jkms.1999.14.5.497
    Database MEDical Literature Analysis and Retrieval System OnLINE

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