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  1. Article ; Online: Synergistic effects of plant genotype and soil microbiome on growth in Lotus japonicus.

    Bamba, Masaru / Akyol, Turgut Yigit / Azuma, Yusuke / Quilbe, Johan / Andersen, Stig Uggerhøj / Sato, Shusei

    FEMS microbiology ecology

    2024  Volume 100, Issue 5

    Abstract: The biological interactions between plants and their root microbiomes are essential for plant growth, and even though plant genotype (G), soil microbiome (M), and growth conditions (environment; E) are the core factors shaping root microbiome, their ... ...

    Abstract The biological interactions between plants and their root microbiomes are essential for plant growth, and even though plant genotype (G), soil microbiome (M), and growth conditions (environment; E) are the core factors shaping root microbiome, their relationships remain unclear. In this study, we investigated the effects of G, M, and E and their interactions on the Lotus root microbiome and plant growth using an in vitro cross-inoculation approach, which reconstructed the interactions between nine Lotus accessions and four soil microbiomes under two different environmental conditions. Results suggested that a large proportion of the root microbiome composition is determined by M and E, while G-related (G, G × M, and G × E) effects were significant but small. In contrast, the interaction between G and M had a more pronounced effect on plant shoot growth than M alone. Our findings also indicated that most microbiome variations controlled by M have little effect on plant phenotypes, whereas G × M interactions have more significant effects. Plant genotype-dependent interactions with soil microbes warrant more attention to optimize crop yield and resilience.
    MeSH term(s) Lotus/microbiology ; Lotus/growth & development ; Lotus/genetics ; Soil Microbiology ; Genotype ; Plant Roots/microbiology ; Plant Roots/growth & development ; Microbiota/genetics ; Soil/chemistry
    Chemical Substances Soil
    Language English
    Publishing date 2024-04-27
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 283722-5
    ISSN 1574-6941 ; 0168-6496
    ISSN (online) 1574-6941
    ISSN 0168-6496
    DOI 10.1093/femsec/fiae056
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Evidence for an RNAi-independent role of Arabidopsis DICER-LIKE2 in growth inhibition and basal antiviral resistance.

    Nielsen, Carsten Poul Skou / Arribas-Hernández, Laura / Han, Lijuan / Reichel, Marlene / Woessmann, Jakob / Daucke, Rune / Bressendorff, Simon / López-Márquez, Diego / Andersen, Stig Uggerhøj / Pumplin, Nathan / Schoof, Erwin M / Brodersen, Peter

    The Plant cell

    2024  

    Abstract: Flowering plant genomes encode four or five DICER-LIKE (DCL) enzymes that produce small interfering RNAs (siRNAs) and microRNAs which function in RNA interference (RNAi). Different RNAi pathways in plants effect transposon silencing, antiviral defense ... ...

    Abstract Flowering plant genomes encode four or five DICER-LIKE (DCL) enzymes that produce small interfering RNAs (siRNAs) and microRNAs which function in RNA interference (RNAi). Different RNAi pathways in plants effect transposon silencing, antiviral defense and endogenous gene regulation. DCL2 acts genetically redundantly with DCL4 to confer basal antiviral defense. However, DCL2 may also counteract DCL4, since knockout of DCL4 causes growth defects that are suppressed by DCL2 inactivation. Current models maintain that RNAi via DCL2-dependent siRNAs is the biochemical basis of both effects. Here, we report that DCL2-mediated antiviral resistance and growth defects cannot be explained by the silencing effects of DCL2-dependent siRNAs. Both functions are defective in genetic backgrounds that maintain high levels of DCL2-dependent siRNAs, either with specific point mutations in DCL2 or with reduced DCL2 dosage because of heterozygosity for dcl2 knockout alleles. Intriguingly, all DCL2 functions require its catalytic activity, and the penetrance of DCL2-dependent growth phenotypes in dcl4 mutants correlates with DCL2 protein levels, but not with levels of major DCL2-dependent siRNAs. We discuss this requirement and correlation with catalytic activity, but not with resulting siRNAs, in light of other findings that reveal a DCL2 function in innate immunity activation triggered by cytoplasmic double-stranded RNA.
    Language English
    Publishing date 2024-03-11
    Publishing country England
    Document type Journal Article
    ZDB-ID 623171-8
    ISSN 1532-298X ; 1040-4651
    ISSN (online) 1532-298X
    ISSN 1040-4651
    DOI 10.1093/plcell/koae067
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Editorial: Molecular and Cellular Mechanisms of the Legume-Rhizobia Symbiosis.

    Wang, Jianping / Andersen, Stig Uggerhøj / Ratet, Pascal

    Frontiers in plant science

    2018  Volume 9, Page(s) 1839

    Language English
    Publishing date 2018-12-12
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2018.01839
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Single-cell analysis identifies genes facilitating rhizobium infection in Lotus japonicus.

    Frank, Manuel / Fechete, Lavinia Ioana / Tedeschi, Francesca / Nadzieja, Marcin / Nørgaard, Malita Malou Malekzadeh / Montiel, Jesus / Andersen, Kasper Røjkjær / Schierup, Mikkel H / Reid, Dugald / Andersen, Stig Uggerhøj

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 7171

    Abstract: Legume-rhizobium signaling during establishment of symbiotic nitrogen fixation restricts rhizobium colonization to specific cells. A limited number of root hair cells allow infection threads to form, and only a fraction of the epidermal infection threads ...

    Abstract Legume-rhizobium signaling during establishment of symbiotic nitrogen fixation restricts rhizobium colonization to specific cells. A limited number of root hair cells allow infection threads to form, and only a fraction of the epidermal infection threads progress to cortical layers to establish functional nodules. Here we use single-cell analysis to define the epidermal and cortical cell populations that respond to and facilitate rhizobium infection. We then identify high-confidence nodulation gene candidates based on their specific expression in these populations, pinpointing genes stably associated with infection across genotypes and time points. We show that one of these, which we name SYMRKL1, encodes a protein with an ectodomain predicted to be nearly identical to that of SYMRK and is required for normal infection thread formation. Our work disentangles cellular processes and transcriptional modules that were previously confounded due to lack of cellular resolution, providing a more detailed understanding of symbiotic interactions.
    MeSH term(s) Rhizobium/metabolism ; Root Nodules, Plant/metabolism ; Lotus/metabolism ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Phenotype ; Symbiosis/genetics ; Single-Cell Analysis ; Gene Expression Regulation, Plant ; Plant Roots/metabolism
    Chemical Substances Plant Proteins
    Language English
    Publishing date 2023-11-07
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-42911-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Widespread and transgenerational retrotransposon activation in inter‐ and intraspecies recombinant inbred populations of Lotus japonicus

    Fukai, Eigo / Yoshikawa, Manabu / Shah, Niraj / Sandal, Niels / Miyao, Akio / Ono, Seijiro / Hirakawa, Hideki / Akyol, Turgut Yigit / Umehara, Yosuke / Nonomura, Ken‐Ichi / Stougaard, Jens / Hirochika, Hirohiko / Hayashi, Makoto / Sato, Shusei / Andersen, Stig Uggerhøj / Okazaki, Keiichi

    plant journal. 2022 Sept., v. 111, no. 5

    2022  

    Abstract: Transposable elements (TEs) constitute a large proportion of genomes of multicellular eukaryotes, including flowering plants. TEs are normally maintained in a silenced state and their transpositions rarely occur. Hybridization between distant species has ...

    Abstract Transposable elements (TEs) constitute a large proportion of genomes of multicellular eukaryotes, including flowering plants. TEs are normally maintained in a silenced state and their transpositions rarely occur. Hybridization between distant species has been regarded as a ‘shock’ that stimulates genome reorganization, including TE mobilization. However, whether crosses between genetically close parents that result in viable and fertile offspring can induce TE transpositions has remained unclear. Here, we investigated the activation of long terminal repeat (LTR) retrotransposons in three Lotus japonicus recombinant inbred line (RIL) populations. We found that at least six LTR retrotransposon families were activated and transposed in 78% of the RILs investigated. LORE1a, one of the transposed LTR retrotransposons, showed transgenerational epigenetic activation, indicating the long‐term effects of epigenetic instability induced by hybridization. Our study highlights TE activation as an unexpectedly common event in plant reproduction.
    Keywords Lotus corniculatus var. japonicus ; epigenetics ; eukaryotic cells ; genome ; hybridization ; inbred lines ; plant reproduction ; progeny ; retrotransposons ; terminal repeat sequences
    Language English
    Dates of publication 2022-09
    Size p. 1397-1410.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 1088037-9
    ISSN 1365-313X ; 0960-7412
    ISSN (online) 1365-313X
    ISSN 0960-7412
    DOI 10.1111/tpj.15896
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: FER and LecRK show haplotype-dependent cold-responsiveness and mediate freezing tolerance in Lotus japonicus.

    Mustamin, Yusdar / Akyol, Turgut Yigit / Gordon, Max / Manggabarani, Andi Madihah / Isomura, Yoshiko / Kawamura, Yasuko / Bamba, Masaru / Williams, Cranos / Andersen, Stig Uggerhøj / Sato, Shusei

    Plant physiology

    2022  Volume 191, Issue 2, Page(s) 1138–1152

    Abstract: Many plant species have succeeded in colonizing a wide range of diverse climates through local adaptation, but the underlying molecular genetics remain obscure. We previously found that winter survival was a direct target of selection during colonization ...

    Abstract Many plant species have succeeded in colonizing a wide range of diverse climates through local adaptation, but the underlying molecular genetics remain obscure. We previously found that winter survival was a direct target of selection during colonization of Japan by the perennial legume Lotus japonicus and identified associated candidate genes. Here, we show that two of these, FERONIA-receptor like kinase (LjFER) and a S-receptor-like kinase gene (LjLecRK), are required for non-acclimated freezing tolerance and show haplotype-dependent cold-responsive expression. Our work suggests that recruiting a conserved growth regulator gene, FER, and a receptor-like kinase gene, LecRK, into the set of cold-responsive genes has contributed to freezing tolerance and local climate adaptation in L. japonicus, offering functional genetic insight into perennial herb evolution.
    MeSH term(s) Lotus/metabolism ; Haplotypes/genetics ; Freezing ; Acclimatization/genetics ; Adaptation, Physiological/genetics ; Gene Expression Regulation, Plant
    Language English
    Publishing date 2022-11-30
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 208914-2
    ISSN 1532-2548 ; 0032-0889
    ISSN (online) 1532-2548
    ISSN 0032-0889
    DOI 10.1093/plphys/kiac533
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Greenotyper: Image-Based Plant Phenotyping Using Distributed Computing and Deep Learning.

    Tausen, Marni / Clausen, Marc / Moeskjær, Sara / Shihavuddin, Asm / Dahl, Anders Bjorholm / Janss, Luc / Andersen, Stig Uggerhøj

    Frontiers in plant science

    2020  Volume 11, Page(s) 1181

    Abstract: Image-based phenotype data with high temporal resolution offers advantages over end-point measurements in plant quantitative genetics experiments, because growth dynamics can be assessed and analysed for genotype-phenotype association. Recently, network- ... ...

    Abstract Image-based phenotype data with high temporal resolution offers advantages over end-point measurements in plant quantitative genetics experiments, because growth dynamics can be assessed and analysed for genotype-phenotype association. Recently, network-based camera systems have been deployed as customizable, low-cost phenotyping solutions. Here, we implemented a large, automated image-capture system based on distributed computing using 180 networked Raspberry Pi units that could simultaneously monitor 1,800 white clover (
    Language English
    Publishing date 2020-08-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2020.01181
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Signaling unmasked: Autophagy and catalase promote programmed cell death.

    Petersen, Morten / Hofius, Daniel / Andersen, Stig Uggerhøj

    Autophagy

    2014  Volume 10, Issue 3, Page(s) 520–521

    Abstract: Autophagy contributes to the removal of harmful cellular refuse, whereas catalase plays an important protective role by detoxifying reactive oxygen species. We recently found that autophagy and catalase are also required for promoting programmed cell ... ...

    Abstract Autophagy contributes to the removal of harmful cellular refuse, whereas catalase plays an important protective role by detoxifying reactive oxygen species. We recently found that autophagy and catalase are also required for promoting programmed cell death induced during plant immune responses. Here we discuss the difficulties in identifying cell death effectors, which are also required to maintain cellular homeostasis, and how their prodeath roles were unmasked using an unbiased forward genetics approach.
    MeSH term(s) Apoptosis/physiology ; Autophagy/physiology ; Catalase/metabolism ; Plants/enzymology ; Reactive Oxygen Species ; Signal Transduction/physiology
    Chemical Substances Reactive Oxygen Species ; Catalase (EC 1.11.1.6)
    Language English
    Publishing date 2014-01-07
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2454135-7
    ISSN 1554-8635 ; 1554-8627
    ISSN (online) 1554-8635
    ISSN 1554-8627
    DOI 10.4161/auto.27564
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Widespread and transgenerational retrotransposon activation in inter- and intraspecies recombinant inbred populations of Lotus japonicus.

    Fukai, Eigo / Yoshikawa, Manabu / Shah, Niraj / Sandal, Niels / Miyao, Akio / Ono, Seijiro / Hirakawa, Hideki / Akyol, Turgut Yigit / Umehara, Yosuke / Nonomura, Ken-Ichi / Stougaard, Jens / Hirochika, Hirohiko / Hayashi, Makoto / Sato, Shusei / Andersen, Stig Uggerhøj / Okazaki, Keiichi

    The Plant journal : for cell and molecular biology

    2022  Volume 111, Issue 5, Page(s) 1397–1410

    Abstract: Transposable elements (TEs) constitute a large proportion of genomes of multicellular eukaryotes, including flowering plants. TEs are normally maintained in a silenced state and their transpositions rarely occur. Hybridization between distant species has ...

    Abstract Transposable elements (TEs) constitute a large proportion of genomes of multicellular eukaryotes, including flowering plants. TEs are normally maintained in a silenced state and their transpositions rarely occur. Hybridization between distant species has been regarded as a 'shock' that stimulates genome reorganization, including TE mobilization. However, whether crosses between genetically close parents that result in viable and fertile offspring can induce TE transpositions has remained unclear. Here, we investigated the activation of long terminal repeat (LTR) retrotransposons in three Lotus japonicus recombinant inbred line (RIL) populations. We found that at least six LTR retrotransposon families were activated and transposed in 78% of the RILs investigated. LORE1a, one of the transposed LTR retrotransposons, showed transgenerational epigenetic activation, indicating the long-term effects of epigenetic instability induced by hybridization. Our study highlights TE activation as an unexpectedly common event in plant reproduction.
    MeSH term(s) Evolution, Molecular ; Genome, Plant/genetics ; Hybridization, Genetic ; Lotus/genetics ; Plants/genetics ; Retroelements/genetics ; Terminal Repeat Sequences/genetics
    Chemical Substances Retroelements
    Language English
    Publishing date 2022-07-30
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1088037-9
    ISSN 1365-313X ; 0960-7412
    ISSN (online) 1365-313X
    ISSN 0960-7412
    DOI 10.1111/tpj.15896
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Genetic analysis of global faba bean diversity, agronomic traits and selection signatures.

    Skovbjerg, Cathrine Kiel / Angra, Deepti / Robertson-Shersby-Harvie, Tom / Kreplak, Jonathan / Keeble-Gagnère, Gabriel / Kaur, Sukhjiwan / Ecke, Wolfgang / Windhorst, Alex / Nielsen, Linda Kærgaard / Schiemann, Andrea / Knudsen, Jens / Gutierrez, Natalia / Tagkouli, Vasiliki / Fechete, Lavinia Ioana / Janss, Luc / Stougaard, Jens / Warsame, Ahmed / Alves, Sheila / Khazaei, Hamid /
    Link, Wolfgang / Torres, Ana Maria / O'Sullivan, Donal Martin / Andersen, Stig Uggerhøj

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

    2023  Volume 136, Issue 5, Page(s) 114

    Abstract: Key message: We identified marker-trait associations for key faba bean agronomic traits and genomic signatures of selection within a global germplasm collection. Faba bean (Vicia faba L.) is a high-protein grain legume crop with great potential for ... ...

    Abstract Key message: We identified marker-trait associations for key faba bean agronomic traits and genomic signatures of selection within a global germplasm collection. Faba bean (Vicia faba L.) is a high-protein grain legume crop with great potential for sustainable protein production. However, little is known about the genetics underlying trait diversity. In this study, we used 21,345 high-quality SNP markers to genetically characterize 2678 faba bean genotypes. We performed genome-wide association studies of key agronomic traits using a seven-parent-MAGIC population and detected 238 significant marker-trait associations linked to 12 traits of agronomic importance. Sixty-five of these were stable across multiple environments. Using a non-redundant diversity panel of 685 accessions from 52 countries, we identified three subpopulations differentiated by geographical origin and 33 genomic regions subjected to strong diversifying selection between subpopulations. We found that SNP markers associated with the differentiation of northern and southern accessions explained a significant proportion of agronomic trait variance in the seven-parent-MAGIC population, suggesting that some of these traits were targets of selection during breeding. Our findings point to genomic regions associated with important agronomic traits and selection, facilitating faba bean genomics-based breeding.
    MeSH term(s) Vicia faba/genetics ; Genome-Wide Association Study ; Plant Breeding ; Phenotype ; Fabaceae/genetics
    Language English
    Publishing date 2023-04-19
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2170-2
    ISSN 1432-2242 ; 0040-5752
    ISSN (online) 1432-2242
    ISSN 0040-5752
    DOI 10.1007/s00122-023-04360-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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