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  1. Article ; Online: Mismatches between Morphology and DNA in Italian Partridges May Not Be Explained Only by Recent Artificial Release of Farm-Reared Birds

    Diego Fontaneto / Paolo Viola / Claudia Pizzirani / Stefania Chiesa / Alessandro Rossetti / Andrea Amici / Livia Lucentini

    Animals, Vol 12, Iss 541, p

    2022  Volume 541

    Abstract: Translocations and releases of farm-reared birds are considered among the major drivers of genetic pollution with consequent loss of genetic diversity in wild populations. In this study, we aimed to assess the extent of hybridization and introgression in ...

    Abstract Translocations and releases of farm-reared birds are considered among the major drivers of genetic pollution with consequent loss of genetic diversity in wild populations. In this study, we aimed to assess the extent of hybridization and introgression in the Italian partridges as a consequence of translocation. We surveyed two mitochondrial markers and one nuclear marker of Alectoris and Perdix from collections (museums and private collections), extant wild populations and farms. Consistent with previous studies, we found haplotypes of allochthonous species within the same genus, likely due to introductions for hunting activities. In addition, we found hybrids between Perdix and Alectoris species with genetic markers from both genera in single individuals. Such introgression was bidirectional and in both mitochondrial and nuclear markers. Counterintuitively, most of the hybrid samples came from collections before the 1950s, when large-scale translocations started, from wild populations where Grey Partridge ( Perdix perdix ) and Rock Partridge ( Alectoris graeca ) overlap in their distribution, whereas only one hybrid occurred among the farmed birds. Our results suggest that Perdix and Alectoris species can hybridize in nature and that artificial translocations and releases of farm-reared birds for restocking or reintroduction purposes may be only partially responsible for the genomic mismatches of Italian partridges.
    Keywords Alectoris graeca ; artificial translocations ; gamebirds ; Perdix perdix ; hybridization ; introgression ; Veterinary medicine ; SF600-1100 ; Zoology ; QL1-991
    Subject code 590
    Language English
    Publishing date 2022-02-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Anthropogenic and Environmental Factors Determining Local Favourable Conditions for Wolves during the Cold Season

    Paolo Viola / Settimio Adriani / Carlo Maria Rossi / Cinzia Franceschini / Riccardo Primi / Marco Apollonio / Andrea Amici

    Animals, Vol 11, Iss 1895, p

    2021  Volume 1895

    Abstract: Winter resources are crucial for wildlife, and, at a local scale, some anthropogenic and environmental factors could affect their availability. In the case of wolves, it is known that vocalisations in response to unfamiliar howls are issued to defend ... ...

    Abstract Winter resources are crucial for wildlife, and, at a local scale, some anthropogenic and environmental factors could affect their availability. In the case of wolves, it is known that vocalisations in response to unfamiliar howls are issued to defend their territory and the important resources within it. Then, we studied the characteristics of winter response sites (WRS) during the cold season, aiming to assess their eventual ability to provide insights into the distribution of valuable resources within their territories. Within this scope, we planned a wolf-howling survey following a standardised approach. The study covered an Apennine (Central Italy) area of 500 km 2 . A hexagonal mesh was imposed on the area, in order to determine the values of different variables at the local scale. A logistic LASSO regression was performed. WRS were positively related to the presence of thermal refuges (odds = 114.485), to patch richness (odds = 1.153), wild boar drive hunting areas (odds = 1.015), and time elapsed since the last hunt (odds = 1.019). Among negative factors, stray dogs reply considerably affects wolves’ responsiveness (odds = 0.207), where odds are the exponentiated coefficients estimated by the logistic lasso regression. These results suggest that WRS are related to anthropogenic and environmental factors favouring the predation process.
    Keywords anthropogenic opportunities ; wolf–free-ranging-dogs interaction ; heat load index ; human disturbance ; resource availability ; thermal refuges ; Veterinary medicine ; SF600-1100 ; Zoology ; QL1-991
    Subject code 333
    Language English
    Publishing date 2021-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: The Effects of Diet Formulation on the Yield, Proximate Composition, and Fatty Acid Profile of the Black Soldier Fly ( Hermetia illucens L.) Prepupae Intended for Animal Feed

    Pier Paolo Danieli / Carola Lussiana / Laura Gasco / Andrea Amici / Bruno Ronchi

    Animals, Vol 9, Iss 4, p

    2019  Volume 178

    Abstract: The black soldier fly (BSF; Hermetia illucens L.) is a very promising insect species due to the ability to convert low-value substrates in highly nutrient feed. This work aimed to study the effect of three nominally isoenergetic diets containing plant ... ...

    Abstract The black soldier fly (BSF; Hermetia illucens L.) is a very promising insect species due to the ability to convert low-value substrates in highly nutrient feed. This work aimed to study the effect of three nominally isoenergetic diets containing plant ingredients such as barley, alfalfa, and wheat byproducts, formulated to be higher in nonfiber carbohydrates (TMD1), fibers (TMD2), and protein (TMD3) in comparison to an extensively genetic modified cereal (cornmeal)-based diet (C), on the growth, yield, and nutritive traits of BSF prepupae (BSFPs). Three growing trials with four biological replicates were carried out. Proximate and fatty acid analyses were performed on the diets and BSFPs. Feed conversion ratios (FCR), dry matter and nutrient yields, and apparent concentration factors (aBCF) for fatty acids (FAs) were calculated. Diet formulation had a substantial effect on the survival, development rate, and larval yield, but the FCR was unaffected. The BSFPs fed TMD3 did not result in a higher crude protein content in comparison to the C or TMD2 diets. Despite the leveled fat content of the diets, BSFPs reared on TMD1 were highest in fat, saturated FA, and fat yield. An apparent bioconcentration factor (aBCF) value lower than unity that was found for the unsaturated FA suggests that the BSFPs inefficiently absorb them from the diet or possibly turn them into saturated FA. However, the unsaturated FA accumulation in BSFPs depended on the levels that were found in the diet, which suggested some possibilities for the FA profile modulation. Overall, the TMD2 performed well despite the low-value of its main ingredients and high fiber content and can be considered to be a feasible option for the mass rearing of BSFPs that are intended for animal feed.
    Keywords low-value feedstocks ; cereal byproducts ; growth performance ; diet optimization ; insects for feed ; fatty acid apparent concentration factor ; Veterinary medicine ; SF600-1100 ; Zoology ; QL1-991
    Subject code 590
    Language English
    Publishing date 2019-04-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: First evidence of heteroplasmy in Grey Partridge (Perdix perdix)

    Claudia Pizzirani / Paolo Viola / Federica Gabbianelli / Anna Fagotti / Francesca Simoncelli / Ines Di Rosa / Paolo Salvi / Andrea Amici / Livia Lucentini

    Avian Research, Vol 11, Iss 1, Pp 1-

    2020  Volume 3

    Abstract: Abstract We report for the first time the occurrence of heteroplasmy in Grey Partridge (Perdix perdix) revealed by means of two mitochondrial fragments. The possible serious biological and management implications of this exception to unilateral ... ...

    Abstract Abstract We report for the first time the occurrence of heteroplasmy in Grey Partridge (Perdix perdix) revealed by means of two mitochondrial fragments. The possible serious biological and management implications of this exception to unilateral inheritance of mtDNA were underlined.
    Keywords Galliformes ; Grey Partridge ; Heteroplasmy ; Hybridization ; Perdix perdix ; Zoology ; QL1-991
    Language English
    Publishing date 2020-08-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Stable isotopes in tissues discriminate the diet of free-living wild boar from different areas of central Italy.

    Giuseppe Russo / Pier Paolo Danieli / Riccardo Primi / Andrea Amici / Marco Lauteri

    PLoS ONE, Vol 12, Iss 8, p e

    2017  Volume 0183333

    Abstract: The use of isotopic signatures in animal tissues provides information on the environment where they are living and, notably, on their diet. Carbon and, whenever possible, nitrogen stable isotope analyses were performed in animal hairs, muscles and fat. ... ...

    Abstract The use of isotopic signatures in animal tissues provides information on the environment where they are living and, notably, on their diet. Carbon and, whenever possible, nitrogen stable isotope analyses were performed in animal hairs, muscles and fat. Particularly, we analyzed both carbon and nitrogen isotopic compositions (δ13C and δ15N) on wild boar samples across three different areas of central Italy (Latium region): Tyrrhenian Coast (TC), Maremma (MA) and Central Plains (CP). The agricultural habits of these areas imply that, in winter, no crops are available for wild boars, which feed mainly on acorns and natural feeds (tubers, earthworms etc.). In addition, the three areas were influenced by oak masting. One of these areas (CP) was characterised by the spreading of corn during the hunting season to attract the animals. For each area, we sampled 10 animals aged between 12 and 24 months and balanced by gender. Anenrichment of δ13C in CP area, where corn was used, was observed in all the analysed tissues in comparison to other areas (MA and TC). In CP area, enriched values of δ15N were also observed in all the tissues. The research demonstrates that both δ13C andδ15N in free-living wild boar tissues are influenced by sampling area. According to feeding habits of the species and wildlife management (feed supplementation), the differences observed in δ13C and δ15Nare based on the specific feeding regime; particularly the use of corn in wintertime. Furthermore, the research highlights and discusses diversities and relationships among δ13C and δ15N in the hair, fat and muscles of free-living wild boar.
    Keywords Medicine ; R ; Science ; Q
    Subject code 333
    Language English
    Publishing date 2017-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: A nesting site suitability model for rock partridge (Alectoris graeca) in the Apennine Mountains using logistic regression

    Lorenzo Boccia / Raffaele Pelorosso / Fioravante Serrani / Andrea Amici

    Italian Journal of Animal Science, Vol 8, Iss 2s, Pp 751-

    2010  Volume 753

    Abstract: The rock partridge has undergone a decline throughout its entire distribution area, including the population of the central Italian Apennine Mountains. Areas of suitable habitat for this species have been reduced due to landscape fragmentation and the ... ...

    Abstract The rock partridge has undergone a decline throughout its entire distribution area, including the population of the central Italian Apennine Mountains. Areas of suitable habitat for this species have been reduced due to landscape fragmentation and the dynamics of domestic animal and wildlife management. The present study was conducted in the Province of Rieti, Lazio Region. Geograph- ical and land use predictors were evaluated in a GIS environment to identify the most relevant factors influencing the presence of rock partridge during the nesting period. Logistic regression was then imple- mented to create a model, characterised by a good level of adequacy, for predicting rock partridge nesting site habitat characteristics. Correct predictions of presence and absence were made in 65.2% and 98.6% of cases, respectively. The ROC value was 0.771, which is statistically significant (P<0.001). The results show that, on a local scale, slope (log), distance from forests, and the presence of bare rocks were statisti- cally significant factors. On a landscape scale, the percentage of forests, the presence of sparse vegetation (over 60%), and a negative Mean Shape Index (MSI) were found to be statistically significant.
    Keywords Rock partridge ; Nesting site ; GIS ; Habitat suitability ; Animal culture ; SF1-1100 ; Agriculture ; S ; DOAJ:Animal Sciences ; DOAJ:Agriculture and Food Sciences
    Language English
    Publishing date 2010-01-01T00:00:00Z
    Publisher PAGEPress Publications
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Simulating Brown hare (Lepus europaeus Pallas) dispersion

    Andrea Amici / Lorenzo Boccia / Raffaele Pelorosso

    Italian Journal of Animal Science, Vol 7, Iss 3, Pp 335-

    a tool for wildlife management of wide areas

    2010  Volume 350

    Abstract: The second half of the 20th century was characterised by intense processes of urbanisation, industrialisation and agricultural mechanisation, leading to a fragmentation of the agricultural and forest landscape. This, in turn, reduced the bio-permeability ...

    Abstract The second half of the 20th century was characterised by intense processes of urbanisation, industrialisation and agricultural mechanisation, leading to a fragmentation of the agricultural and forest landscape. This, in turn, reduced the bio-permeability of the territory and affected the dispersion of many wild species. Brown hare (Lepus europeus) dispersion is dramatically affected by habitat fragmentation, presence of predators, intense tillage and elevated hunting pressure. Consequently, the only stable populations of hare are often in no-hunting areas where wildlife management is efficient. It is necessary, therefore, to identify not only additional areas suitable for reproduction, but also the most suitable dispersion pathways for hares, in order to optimise management. In the present study, by means of a Geographic Information System (GIS), a deterministic hare suitability model was developed on the basis of a multicriterial approach and fuzzy logic. Subsequently, a friction surface was derived from the suitability map in order to describe the land bio-permeability. Finally, on the basis of species potential, the spread of hares from stable population areas (source areas) to the remaining territory was simulated. The area of study was the province of Viterbo (central Italy). The suitability map showed good discrimination ability (ROC=0.705). The hare dispersion simulation map allowed the potential spreading of this species throughout the provincial territory to be analysed. Isolated or less connected zones were highlighted, allowing the distribution of habitat enhancements, and/or the institution of new no-hunting areas devoted to the reproduction and consequent spread of hares throughout the territory, to be localised. The presented flexible and reiterable methodology could prove useful for wildlife management and hunting planning over a wide area. It would thus provide an important contribution to reducing the importance of animal translocation and favouring an increase in native resources spontaneously spreading throughout a territory. In a more general sense, this study is in accordance with the sustainable land management perspective, meeting the requirements of environmental protection, without compromising the anthropic development of non-urban areas.
    Keywords Landscape fragmentation ; Multicriterial evaluation ; Wildlife management ; Brown hare ; Dispersion. ; Animal culture ; SF1-1100 ; Agriculture ; S ; DOAJ:Animal Sciences ; DOAJ:Agriculture and Food Sciences
    Subject code 710
    Language English
    Publishing date 2010-01-01T00:00:00Z
    Publisher PAGEPress Publications
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Somatic variability in wild boar (Sus scrofa L.) in different areas of Central Italy

    Settimio Adriani / Fioravante Serrani / Andrea Amici

    Italian Journal of Animal Science, Vol 9, Iss 1, Pp e9-e

    2010  Volume 9

    Abstract: A survey of wild boar shot during two consecutive years (hunting seasons 2002-2004) was carried out in order to evaluate which somatic measurements are most significant in identifying and discriminating among different morphotypes in central Italy. ... ...

    Abstract A survey of wild boar shot during two consecutive years (hunting seasons 2002-2004) was carried out in order to evaluate which somatic measurements are most significant in identifying and discriminating among different morphotypes in central Italy. Biometric data from 688 wild boars was collected in three different areas of central Italy, two in Viterbo and one in the Province of Rieti. The following somatic measurements were individually recorded for each specimen: head-body length, height at withers, hind-foot length, ear length, ear-snout distance and ear-shoulder distance. Body weight was registered, and age was estimated from tooth eruption and wear. The animals were divided into three age classes; young (aged less then 12 months), sub-adults (aged between 12 and 36 months), and adults (36 months and older). After a preliminary ANOVA procedure, which did not give satisfactory results, a statistical analysis was performed using a canonical discriminant procedure, given an a priori classification (geographical area) and several quantitative variables (somatic measurements and weight). The separation between areas was estimated calculating the squared distance of Mahalanobis. The data referring to all 688 specimens was subjected to factor analysis. The results of the canonical discriminant analysis highlight the existence of two distinct groups within all three age classes. There is a statistically significant difference between the southern- Maremma (SM) vs. the Apennine (A) and sub- Apennine (SA) areas, for young (P<0.0001), sub-adults (P<0.001) and adults (P<0.001). The difference between the A and SA areas was significant only for sub-adults (P<0.05). The first canonical variable account for 92.5, 92.7 and 89.9% of the total variance for the three age classes respectively, but this is unequally correlated with the original variables suggesting that the separation between the two areas is due to differences in conformation rather than in body size. On the basis of the discriminant analysis large part of the animals were correctly categorised in the sampling areas. As regards the factor analysis results for the adult group, the first three common factors are able to explain 78, 92, and 64% of the covariance for the data of the SM, A and SA groups respectively. These results suggest that, for the SM group, a differentiation among morphotypes may be possible on the basis of a few somatic measurements. These results confirm the need for biochemical and genetic studies to identify if the different morphotypes refer to the autochthonous wild boar strain.
    Keywords Wild boar ; Sus scrofa ; Morphology ; Somatic measurements ; Ecotype. ; Animal culture ; SF1-1100 ; Agriculture ; S ; DOAJ:Animal Sciences ; DOAJ:Agriculture and Food Sciences
    Subject code 630
    Language English
    Publishing date 2010-02-01T00:00:00Z
    Publisher PAGEPress Publications
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: A statistical GIS-based analysis of Wild boar (Sus scrofa) traffic collisions in a Mediterranean area

    Andrea Amici / Riccardo Primi / Raffaele Pelorosso / Maria Nicolina Ripa

    Italian Journal of Animal Science, Vol 8, Iss 2s, Pp 649-

    2010  Volume 651

    Abstract: vThis study was aimed at analysing the most relevant environmental variables involved in vehicle–wild boar road collisions in a Mediterranean area, starting from a territorial information system implemented in a GIS environment. Landscape structure ... ...

    Abstract vThis study was aimed at analysing the most relevant environmental variables involved in vehicle–wild boar road collisions in a Mediterranean area, starting from a territorial information system implemented in a GIS environment. Landscape structure indices and local qualitative and quantitative variables were correlated to identify the most frequent predisposing factors of collisions. Statistical tests of the considered parameters indicated a higher frequency of collisions in the evening hours of late summer and autumn (P<0.05) compared with daytime and night-time, localized nearness to attraction points (feeding or resting sites; P<0.001) and closeness to no-hunting areas (P<0.001). In addition, local road parameters (shape of road, visibility at road sides, etc.) considerably increased the risk of collisions.
    Keywords Wild boar ; Road collision ; GIS ; Landscape structure ; Animal culture ; SF1-1100 ; Agriculture ; S ; DOAJ:Animal Sciences ; DOAJ:Agriculture and Food Sciences
    Language English
    Publishing date 2010-01-01T00:00:00Z
    Publisher PAGEPress Publications
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Concentrations of some toxic and trace elements in wild boar (Sus scrofa) organs and tissues in different areas of the Province of Viterbo (Central Italy)

    Bruno Ronchi / Riccardo Primi / Claudia Russo / Pier Paolo Danieli / Andrea Amici

    Italian Journal of Animal Science, Vol 11, Iss 4, Pp e65-e

    2012  Volume 65

    Abstract: The aim of the present study was to determine heavy metal reference levels for exposure and risk assessment studies on a local scale. The average levels of cadmium recorded in 75 wild boars were 0.085, 0.079 and 1.052 mg Cd kg -1 wet weight (w.w.) in the ...

    Abstract The aim of the present study was to determine heavy metal reference levels for exposure and risk assessment studies on a local scale. The average levels of cadmium recorded in 75 wild boars were 0.085, 0.079 and 1.052 mg Cd kg -1 wet weight (w.w.) in the liver, muscle and kidney, respectively. The majority of the muscle samples and some of the liver samples contained levels of heavy metal that were over the legal limit [EU Maximum Residue Levels (MRLs)] for pigs. Our data are similar to or lower than the values reported in most of the available literature. For Pb concentration, the average values recorded were 0.318, 0.126 and 0.298 mg kg kg -1 ww.w. in the liver, muscle and kidney, respectively. The samples that were non-compliant with regulatory limits (MRLs) for pigs were registered only for muscle. Available data on the presence of Pb content in game meat report lower values than ours, most likely because the area around the bullet path was avoided while sampling. The average values of total Cr were 0.141, 0.139 and 0.097 mg Cr kg -1 w.w. in the liver, muscle and kidney, respectively. For Zn, the mean values were 49.76, 53.21 and 32.46 mg kg -1 w.w. in liver, muscle and kidney, respectively. Cu content was 46.12, 12.20 and 5.64 mg kg -1 w.w. in the liver, muscle and kidney, respectively. The results obtained have been validated on the basis of the scarce and inconsistent Italian literature available and on international studies.
    Keywords Heavy metals ; Trace elements ; Wild boar ; Edible tissues ; Province of Viterbo ; Animal culture ; SF1-1100 ; Agriculture ; S ; DOAJ:Animal Sciences ; DOAJ:Agriculture and Food Sciences
    Subject code 333
    Language English
    Publishing date 2012-10-01T00:00:00Z
    Publisher PAGEPress Publications
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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