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  1. AU="Andreko, Susan K"
  2. AU="Ames, DeWayne"
  3. AU="Fokom Domgue, Joel"
  4. AU="Soubani, Ayman O"
  5. AU="Weir, Andrew"
  6. AU="McGowan, Alessia"
  7. AU=Hoepler Wolfgang AU=Hoepler Wolfgang
  8. AU="Pintér, Nándor K"
  9. AU=Linask Kersti K
  10. AU="Arya, Akanksha"
  11. AU="Jue, Nathaniel"
  12. AU="Favaro, Enrica"
  13. AU="Santana, Margarida M"
  14. AU="Wiegand, Ryan E"
  15. AU="Cosio, Daniela S"
  16. AU="Yasuda, Michiyuki"
  17. AU="Theodoratou, Evropi"
  18. AU="Ernfors, Patrik"
  19. AU="Pingel, Simon"
  20. AU="W. T. Lawrence"
  21. AU="Tietzmann, Marcel"
  22. AU="DeRenzo, Christopher"

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  1. Artikel ; Online: Near-instant surface-selective fluorogenic protein quantification using sulfonated triarylmethane dyes and fluorogen activating proteins.

    Yan, Qi / Schmidt, Brigitte F / Perkins, Lydia A / Naganbabu, Matharishwan / Saurabh, Saumya / Andreko, Susan K / Bruchez, Marcel P

    Organic & biomolecular chemistry

    2015  Band 13, Heft 7, Seite(n) 2078–2086

    Abstract: Agonist-promoted G-protein coupled receptor (GPCR) endocytosis and recycling plays an important role in many signaling events in the cell. However, the approaches that allow fast and quantitative analysis of such processes still remain limited. Here we ... ...

    Abstract Agonist-promoted G-protein coupled receptor (GPCR) endocytosis and recycling plays an important role in many signaling events in the cell. However, the approaches that allow fast and quantitative analysis of such processes still remain limited. Here we report an improved labeling approach based on the genetic fusion of a fluorogen activating protein (FAP) to a GPCR and binding of a sulfonated analog of the malachite green (MG) fluorogen to rapidly and selectively label cell surface receptors. Fluorescence microscopy and flow cytometry demonstrate that this dye does not cross the plasma membrane, binds with high affinity to a dL5** FAP-GPCR fusion construct, activating tagged surface receptors within seconds of addition. The ability to rapidly and selectively label cell surface receptors with a fluorogenic genetically encoded tag allows quantitative imaging and analysis of highly dynamic processes like receptor endocytosis and recycling.
    Mesh-Begriff(e) Cells, Cultured ; Flow Cytometry ; Fluorescent Dyes/chemistry ; HEK293 Cells ; HeLa Cells ; Humans ; Methane/chemistry ; Microscopy, Fluorescence ; Molecular Structure ; Proteins/chemistry ; Proteins/genetics ; Receptors, G-Protein-Coupled/chemistry ; Surface Properties
    Chemische Substanzen Fluorescent Dyes ; Proteins ; Receptors, G-Protein-Coupled ; Methane (OP0UW79H66)
    Sprache Englisch
    Erscheinungsdatum 2015-02-21
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2097583-1
    ISSN 1477-0539 ; 1477-0520
    ISSN (online) 1477-0539
    ISSN 1477-0520
    DOI 10.1039/c4ob02309a
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Genetically targeted fluorogenic macromolecules for subcellular imaging and cellular perturbation.

    Magenau, Andrew J D / Saurabh, Saumya / Andreko, Susan K / Telmer, Cheryl A / Schmidt, Brigitte F / Waggoner, Alan S / Bruchez, Marcel P

    Biomaterials

    2015  Band 66, Seite(n) 1–8

    Abstract: The alteration of cellular functions by anchoring macromolecules to specified organelles may reveal a new area of therapeutic potential and clinical treatment. In this work, a unique phenotype was evoked by influencing cellular behavior through the ... ...

    Abstract The alteration of cellular functions by anchoring macromolecules to specified organelles may reveal a new area of therapeutic potential and clinical treatment. In this work, a unique phenotype was evoked by influencing cellular behavior through the modification of subcellular structures with genetically targetable macromolecules. These fluorogen-functionalized polymers, prepared via controlled radical polymerization, were capable of exclusively decorating actin, cytoplasmic, or nuclear compartments of living cells expressing localized fluorgen-activating proteins. The macromolecular fluorogens were optimized by establishing critical polymer architecture-biophysical property relationships which impacted binding rates, binding affinities, and the level of internalization. Specific labeling of subcellular structures was realized at nanomolar concentrations of polymer, in the absence of membrane permeabilization or transduction domains, and fluorogen-modified polymers were found to bind to protein intact after delivery to the cytosol. Cellular motility was found to be dependent on binding of macromolecular fluorogens to actin structures causing rapid cellular ruffling without migration.
    Mesh-Begriff(e) Fluorescent Dyes/chemistry ; Fluorescent Dyes/pharmacokinetics ; Gene Targeting/methods ; HeLa Cells ; Humans ; Microscopy, Fluorescence/methods ; Molecular Imaging/methods ; Proteins/chemistry ; Proteins/genetics ; Proteins/pharmacokinetics ; Subcellular Fractions/metabolism ; Subcellular Fractions/ultrastructure
    Chemische Substanzen Fluorescent Dyes ; Proteins
    Sprache Englisch
    Erscheinungsdatum 2015-10
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 603079-8
    ISSN 1878-5905 ; 0142-9612
    ISSN (online) 1878-5905
    ISSN 0142-9612
    DOI 10.1016/j.biomaterials.2015.07.002
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel: Genetically targeted fluorogenic macromolecules for subcellular imaging and cellular perturbation

    Magenau, Andrew J.D / Saurabh, Saumya / Andreko, Susan K / Telmer, Cheryl A / Schmidt, Brigitte F / Waggoner, Alan S / Bruchez, Marcel P

    Biomaterials. 2015 Oct., v. 66

    2015  

    Abstract: The alteration of cellular functions by anchoring macromolecules to specified organelles may reveal a new area of therapeutic potential and clinical treatment. In this work, a unique phenotype was evoked by influencing cellular behavior through the ... ...

    Abstract The alteration of cellular functions by anchoring macromolecules to specified organelles may reveal a new area of therapeutic potential and clinical treatment. In this work, a unique phenotype was evoked by influencing cellular behavior through the modification of subcellular structures with genetically targetable macromolecules. These fluorogen-functionalized polymers, prepared via controlled radical polymerization, were capable of exclusively decorating actin, cytoplasmic, or nuclear compartments of living cells expressing localized fluorgen-activating proteins. The macromolecular fluorogens were optimized by establishing critical polymer architecture-biophysical property relationships which impacted binding rates, binding affinities, and the level of internalization. Specific labeling of subcellular structures was realized at nanomolar concentrations of polymer, in the absence of membrane permeabilization or transduction domains, and fluorogen-modified polymers were found to bind to protein intact after delivery to the cytosol. Cellular motility was found to be dependent on binding of macromolecular fluorogens to actin structures causing rapid cellular ruffling without migration.
    Schlagwörter actin ; binding capacity ; cell movement ; cytosol ; image analysis ; membrane permeability ; organelles ; phenotype ; polymerization ; polymers ; therapeutics
    Sprache Englisch
    Erscheinungsverlauf 2015-10
    Umfang p. 1-8.
    Erscheinungsort Elsevier Ltd
    Dokumenttyp Artikel
    ZDB-ID 603079-8
    ISSN 0142-9612
    ISSN 0142-9612
    DOI 10.1016/j.biomaterials.2015.07.002
    Datenquelle NAL Katalog (AGRICOLA)

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    Verfügbar in ZB MED Köln/Königswinter

    Zs.B 2371: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  4. Artikel ; Online: Nanoparticle transport from mouse vagina to adjacent lymph nodes.

    Ballou, Byron / Andreko, Susan K / Osuna-Highley, Elvira / McRaven, Michael / Catalone, Tina / Bruchez, Marcel P / Hope, Thomas J / Labib, Mohamed E

    PloS one

    2012  Band 7, Heft 12, Seite(n) e51995

    Abstract: To test the feasibility of localized intravaginal therapy directed to neighboring lymph nodes, the transport of quantum dots across the vaginal wall was investigated. Quantum dots instilled into the mouse vagina were transported across the vaginal mucosa ...

    Abstract To test the feasibility of localized intravaginal therapy directed to neighboring lymph nodes, the transport of quantum dots across the vaginal wall was investigated. Quantum dots instilled into the mouse vagina were transported across the vaginal mucosa into draining lymph nodes, but not into distant nodes. Most of the particles were transported to the lumbar nodes; far fewer were transported to the inguinal nodes. A low level of transport was evident at 4 hr after intravaginal instillation, and transport peaked at about 36 hr after instillation. Transport was greatly enhanced by prior vaginal instillation of Nonoxynol-9. Hundreds of micrograms of nanoparticles/kg tissue (ppb) were found in the lumbar lymph nodes at 36 hr post-instillation. Our results imply that targeted transport of microbicides or immunogens from the vagina to local lymph organs is feasible. They also offer an in vivo model for assessing the toxicity of compounds intended for intravaginal use.
    Mesh-Begriff(e) Administration, Intravaginal ; Animals ; Antigens/administration & dosage ; Antigens/immunology ; Cadmium ; Drug Delivery Systems ; Female ; Kinetics ; Lumbar Vertebrae ; Lymph Nodes/immunology ; Lymph Nodes/metabolism ; Mice ; Molecular Imaging ; Nanoparticles/administration & dosage ; Quantum Dots ; Vagina/immunology ; Vagina/metabolism
    Chemische Substanzen Antigens ; Cadmium (00BH33GNGH)
    Sprache Englisch
    Erscheinungsdatum 2012-12-21
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0051995
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel ; Online: Long-term persistence and spectral blue shifting of quantum dots in vivo.

    Fitzpatrick, James A J / Andreko, Susan K / Ernst, Lauren A / Waggoner, Alan S / Ballou, Byron / Bruchez, Marcel P

    Nano letters

    2009  Band 9, Heft 7, Seite(n) 2736–2741

    Abstract: Quantum dots are a powerful fluorophore family with desirable attributes for fluorescence imaging. They have been used in several animal models with direct clinical relevance, including sentinel lymph node mapping, tracing vasculature and lymphatics, and ...

    Abstract Quantum dots are a powerful fluorophore family with desirable attributes for fluorescence imaging. They have been used in several animal models with direct clinical relevance, including sentinel lymph node mapping, tracing vasculature and lymphatics, and targeting specific lesions for diagnosis and removal. (1-12) Despite significant interest for use in translational applications, little is known about the persistence and long-term fate of quantum dots in vivo. We have observed fluorescence of quantum dots injected into Balb/c and nude mice for up to two-years post injection using both whole-body and microscopic fluorescence techniques. Two-photon spectral microscopy was used to verify the existence of quantum dots within two-year tissues, but also revealed a range of significantly blue-shifted emission peaks with increased bandwidths. Systemically administered quantum dots persist and retain fluorescence for up to two-years in vivo, but with significantly blue-shifted emission.
    Mesh-Begriff(e) Animals ; Fluorescent Dyes/chemistry ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Microscopy, Electron, Transmission ; Quantum Dots ; Time Factors
    Chemische Substanzen Fluorescent Dyes
    Sprache Englisch
    Erscheinungsdatum 2009-06-11
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ISSN 1530-6992
    ISSN (online) 1530-6992
    DOI 10.1021/nl901534q
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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