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  1. Article ; Online: Controlled pore anisotropy in chitosan-gelatin cryogels for use in bone tissue engineering.

    Andres, Madeline / Robertson, Eileen / Hall, Andrew / McBride-Gagyi, Sarah / Sell, Scott

    Journal of biomaterials applications

    2024  Volume 38, Issue 7, Page(s) 797–807

    Abstract: In tissue engineering, the development of an appropriate scaffold is crucial to provide a framework for new tissue growth. The use of cryogels as scaffolds shows promise due to their macroporous structure, but the pore size, distribution, and ... ...

    Abstract In tissue engineering, the development of an appropriate scaffold is crucial to provide a framework for new tissue growth. The use of cryogels as scaffolds shows promise due to their macroporous structure, but the pore size, distribution, and interconnectivity is highly variable depending on the fabrication process. The objective of the current research is to provide a technique for controlled anisotropy in chitosan-gelatin cryogels to develop scaffolds for bone tissue engineering application. A mold was developed using additive manufacturing to be used during the freezing process in order to fabricate cryogels with a more interconnected pore structure. The scaffolds were tested to evaluate their porosity, mechanical strength, and to observe cell infiltration through the cryogel. It was found that the use of the mold allowed for the creation of designated pores within the cryogel structure which facilitated cell infiltration to the center of the scaffold without sacrificing mechanical integrity of the structure.
    MeSH term(s) Tissue Engineering/methods ; Cryogels/chemistry ; Tissue Scaffolds/chemistry ; Chitosan/chemistry ; Gelatin/chemistry ; Anisotropy ; Porosity
    Chemical Substances Cryogels ; Chitosan (9012-76-4) ; Gelatin (9000-70-8)
    Language English
    Publishing date 2024-01-26
    Publishing country England
    Document type Journal Article
    ZDB-ID 639283-0
    ISSN 1530-8022 ; 0885-3282
    ISSN (online) 1530-8022
    ISSN 0885-3282
    DOI 10.1177/08853282231222324
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Cellular microRNA detection with miRacles: microRNA- activated conditional looping of engineered switches.

    Chandrasekaran, Arun Richard / MacIsaac, Molly / Dey, Paromita / Levchenko, Oksana / Zhou, Lifeng / Andres, Madeline / Dey, Bijan K / Halvorsen, Ken

    Science advances

    2019  Volume 5, Issue 3, Page(s) eaau9443

    Abstract: MicroRNAs are short noncoding regulatory RNAs that are increasingly used as disease biomarkers. Detection of microRNAs can be arduous and expensive and often requires amplification, labeling, or radioactive probes. Here, we report a single-step, ... ...

    Abstract MicroRNAs are short noncoding regulatory RNAs that are increasingly used as disease biomarkers. Detection of microRNAs can be arduous and expensive and often requires amplification, labeling, or radioactive probes. Here, we report a single-step, nonenzymatic microRNA detection assay using conformationally responsive DNA nanoswitches. Termed miRacles (microRNA-activated conditional looping of engineered switches), our assay has subattomole sensitivity and single-nucleotide specificity using an agarose gel electrophoresis readout. We detect cellular microRNAs from nanogram-scale RNA extracts of differentiating muscle cells and multiplex our detection for several microRNAs from one biological sample. We demonstrate 1-hour detection without expensive equipment or reagents, making this assay a compelling alternative to quantitative polymerase chain reaction and Northern blotting.
    MeSH term(s) Animals ; Base Pairing ; Cell Differentiation ; Cell Line ; DNA, Single-Stranded/genetics ; DNA, Single-Stranded/metabolism ; Electrophoresis, Agar Gel/methods ; Electrophoresis, Agar Gel/standards ; Fluorescent Dyes/chemistry ; Genetic Engineering/methods ; Humans ; Intercalating Agents/chemistry ; Inverted Repeat Sequences ; Mice ; MicroRNAs/analysis ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Myoblasts/cytology ; Myoblasts/metabolism ; Sensitivity and Specificity
    Chemical Substances DNA, Single-Stranded ; Fluorescent Dyes ; Intercalating Agents ; MicroRNAs
    Language English
    Publishing date 2019-03-13
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2810933-8
    ISSN 2375-2548 ; 2375-2548
    ISSN (online) 2375-2548
    ISSN 2375-2548
    DOI 10.1126/sciadv.aau9443
    Database MEDical Literature Analysis and Retrieval System OnLINE

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