LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 7 of total 7

Search options

  1. Article ; Online: Congenital Cystic Adenomatoid Malformation

    Asmita Anilkumar Mehta / Naveen Viswanathan / Anil Kumar Vasudevan / Roopa Paulose / Mohan Abraham

    Journal of Clinical and Diagnostic Research, Vol 10, Iss 11, Pp SC01-SC

    A Tertiary Care Hospital Experience

    2016  Volume 04

    Abstract: Introduction: Congenital Cystic Adenomatoid Malformation (CCAM) is an uncommon developmental deformity affecting the terminal respiratory structures. It is characterized by broncho pulmonary foregut malformations. The reason behind it is an arrest in ... ...

    Abstract Introduction: Congenital Cystic Adenomatoid Malformation (CCAM) is an uncommon developmental deformity affecting the terminal respiratory structures. It is characterized by broncho pulmonary foregut malformations. The reason behind it is an arrest in lung development between 4th and 7th week of fetal life. Aim: The present study was conducted to assess the clinical and radiological profile and also to study the role of surgical intervention in patients with CCAM. Materials and Methods: All patients with clinical suspicion or provisional diagnosis of CCAM were included in the study. A clinical questionnaire was prepared to collect data. Computed Tomography (CT) chest with High Resolution Computed Tomography (HRCT) was done for all the patients. Patients were assessed by paediatric surgeon and eligible patients were operated. The procedure conducted was usually open thoracotomy under general anaesthesia. The affected lobes were removed and specimens were sent for histopathological analysis. All included patients were followed up prospectively to find out about their current level of health. Via telephonic interview they were asked about their overall growth, quality of life, activity, rate of respiratory infections and requirement of hospital admission. Results: Total 15 patients with diagnosis of CCAM were included in the study. Of them, 8 (53.3%) were male. The commonest presentation was cough 13(86%), Breathing difficulty 11(73%), fever 9(60%), recurrent pneumonia 4(26%), hypoxia requiring oxygen supplementation 6(40%), others 2(12%). Thirteen patients required surgical intervention and underwent lobectomy. There were 2 cases of type I, one each of type II and III, 3 case of type IV while 5 were intermediate type. There was no procedure related mortality. The median duration of hospital stay and all were successfully discharged with median duration of stay 11 ± 16 days. Conclusion: The study concludes that if recognized early, surgical removal of affected lung prevents the complications like recurrent pulmonary infections. The surgery is well tolerated without any post-operative mortality or morbidity.
    Keywords bronchopulmonary foregut malformation ; pulmonary infection ; surgical resection ; Medicine ; R
    Subject code 610
    Language English
    Publishing date 2016-11-01T00:00:00Z
    Publisher JCDR Research and Publications Private Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article: Understanding the adhesion mechanism of a mucin binding domain from Lactobacillus fermentum and its role in enteropathogen exclusion

    Chatterjee, Maitrayee / Anil Kumar Vasudevan / Anju Choorakottayil Pushkaran / Chethampadi Gopi Mohan / Krishna Kumar N. Menon / Raja Biswas

    International journal of biological macromolecules. 2017,

    2017  

    Abstract: Lactobacillus species possesses surface exposed Mucin Binding Protein (MucBP) which plays a role in adhesion to gastrointestinal mucin. MucBP contains one or more mucin binding domain (MBD), the functionality of which has yet not been characterized ... ...

    Abstract Lactobacillus species possesses surface exposed Mucin Binding Protein (MucBP) which plays a role in adhesion to gastrointestinal mucin. MucBP contains one or more mucin binding domain (MBD), the functionality of which has yet not been characterized thoroughly. Here, we have characterized a 93-amino acid MBD (MBD93) of MucBP (LAF_0673) from Lactobacillus fermentum. Multiple sequence alignment of L. fermentum MBD93 exhibited ∼60% sequence homology with MBDs from other Lactobacillus species. Further, we cloned, expressed and purified MBD93 from Escherichia coli as N-terminal histidine-tagged protein (6X His-MBD93). The purified MBD93 was able to bind to mucin and showed strong affinity towards the terminally expressed mucin glycans viz. N-acetylgalactosamine (GalNAc), N-acetylglucosamine (GlcNAc), Galactose (Gal), and Sialic acid (N-acetylneuraminic acid; Neu5Ac). In silico experiments further confirmed the interaction between homology modeled MBD93 to mucin glycans through hydrogen-bonding with its surface amino acid residues Ser57, Pro58, Ile60, Tyr63 and Ala65. We also have demonstrated that MBD93 was able to inhibit the adhesion of enteric pathogens, including E. coli, Salmonella Paratyphi A, Shigella sonnei and Proteus vulgaris to mucin. Our results suggested that L. fermentum MBD93 is a functionally sufficient unit to act as an adhesin and to protect from invading enteric pathogens.
    Keywords adhesins ; adhesion ; amino acids ; binding proteins ; enteropathogens ; Escherichia coli ; galactose ; gastrointestinal system ; hydrogen bonding ; Lactobacillus fermentum ; mucins ; N-acetylglucosamine ; polysaccharides ; Proteus vulgaris ; Salmonella Paratyphi A ; sequence alignment ; sequence homology ; Shigella sonnei ; sialic acid
    Language English
    Size p. .
    Publishing place Elsevier B.V.
    Document type Article
    Note Pre-press version
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2017.10.107
    Database NAL-Catalogue (AGRICOLA)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 2374: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: Combination of Repurposed Drug Diosmin with Amoxicillin-Clavulanic acid Causes Synergistic Inhibition of Mycobacterial Growth

    Anju Choorakottayil Pushkaran / Vivek Vinod / Muralidharan Vanuopadath / Sudarslal Sadasivan Nair / Shantikumar V. Nair / Anil Kumar Vasudevan / Raja Biswas / Chethampadi Gopi Mohan

    Scientific Reports, Vol 9, Iss 1, Pp 1-

    2019  Volume 14

    Abstract: Abstract Effective therapeutic regimens for the treatment of tuberculosis (TB) are limited. They are comprised of multiple drugs that inhibit the essential cellular pathways in Mycobacterium tuberculosis (Mtb). The present study investigates an approach ... ...

    Abstract Abstract Effective therapeutic regimens for the treatment of tuberculosis (TB) are limited. They are comprised of multiple drugs that inhibit the essential cellular pathways in Mycobacterium tuberculosis (Mtb). The present study investigates an approach which enables a combination of Amoxicillin-Clavulanic acid (AMC) and a repurposed drug for its synergistic effect towards TB treatment. We identified Diosmin (DIO), by targeting the active site residues of L,D-transpeptidase (Ldt) enzymes involved in Mtb cell wall biosynthesis by using a structure-based drug design method. DIO is rapidly converted into aglycone form Diosmetin (DMT) after oral administration. Binding of DIO or DMT towards Ldt enzymes was studied using molecular docking and bioassay techniques. Combination of DIO (or DMT) and AMC exhibited higher mycobactericidal activity against Mycobacterium marinum as compared to individual drugs. Scanning electron microscopy study of M. marinum treated with AMC-DIO and AMC-DMT showed marked cellular leakage. M. marinum infected Drosophila melanogaster fly model showed an increased fly survival of ~60% upon treatment with a combination of AMC and DIO (or DMT). Finally, the enhanced in vitro antimicrobial activity of AMC-DIO was validated against Mtb H37Ra and a MDR clinical isolate. Our results demonstrate the potential for AMC and DIO (or DMT) as a synergistic combination for the treatment of TB.
    Keywords Medicine ; R ; Science ; Q
    Subject code 572
    Language English
    Publishing date 2019-05-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  4. Book ; Online: New Particle Formation at a High Altitude Site in India

    Singla, Vyoma / Mukherjee, Subrata / Kristensson, Adam / Pandithurai, Govindan / Dani, Kundan K. / Anil Kumar, Vasudevan

    eISSN: 1680-7324

    Impact of Fresh Emissions and Long Range Transport

    2018  

    Abstract: There is a lack of characterization of the aerosol population in Western India, how it is affected by meteorological parameters, and new particle formation and the influence on cloud condensation nuclei (CCN). For this reason, measurements of particle ... ...

    Abstract There is a lack of characterization of the aerosol population in Western India, how it is affected by meteorological parameters, and new particle formation and the influence on cloud condensation nuclei (CCN). For this reason, measurements of particle number size distribution, aerosol chemical composition, meteorology and cloud condensation nuclei number concentration were monitored at High Altitude Cloud Physics Laboratory (HACPL) in Mahabaleshwar mountain town in Western India between November 2016 and February 2017. Most air masses in this period originated from the Indian continent to the north-east of HACPL. New particle formation (NPF) events were observed on 47 days and mainly associated with these north-easterly air masses and high SO 2 emissions and biomass burning activities, while weaker or non-NPF days were associated with westerly air masses and relatively higher influence of local air pollution. The growth of newly formed particles enhanced the mass concentration of secondary organic and inorganic species of aerosol particles. The mean growth rate, formation rate, condensation sink and coagulation loss for the 13 strongest events was found to be 2.58 ± 0.38 nm h −1 , 2.82 ± 1.37 cm −3 s −1 , 22.3 ± 2.87 * 10-3 s −1 and 1.62 ± 1.04 cm −3 s −1 respectively. A closer examination of 5 events showed that low relative humidity and solar radiation favoured new particle formation. These NPF events lead to a significant increase in CCN concentration (mean ~ 53 ± 36 %). The NanoMap method revealed that NPF took place up to several hundred kilometers upwind and to the north-east of HACPL.
    Subject code 333
    Language English
    Publishing date 2018-07-02
    Publishing country de
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article: Bioadhesive, Hemostatic, and Antibacterial in Situ Chitin–Fibrin Nanocomposite Gel for Controlling Bleeding and Preventing Infections at Mediastinum

    Sundaram, M. Nivedhitha / Anil Kumar Vasudevan / Maneesha K. Suresh / Praveen Kerala Varma / R. Jayakumar / Raja Biswas / Vignesh Krishnamoorthi Kaliannagounder / Vignesh Selvaprithiviraj

    ACS sustainable chemistry & engineering. 2018 Apr. 20, v. 6, no. 6

    2018  

    Abstract: Mediastinitis occurs after cardiac surgery and is a major threat to patient’s life due to postoperative bleeding and deep sternal wound infection. Major challenge in treating this condition is that it demands a material that should adhere to the applied ... ...

    Abstract Mediastinitis occurs after cardiac surgery and is a major threat to patient’s life due to postoperative bleeding and deep sternal wound infection. Major challenge in treating this condition is that it demands a material that should adhere to the applied site and act as both a hemostatic and an antibacterial agent. On the basis of this we have developed an in situ forming tissue adhesive chitin–fibrin (CH-FB) gel with tigecycline loaded gelatin nanoparticles (tGNPs) for controlling bleeding and preventing bacterial infection. Spherical shaped tGNPs (231 ± 20 nm) were prepared and characterized. In situ forming tGNPsCH-FB gel was formed using a dual syringe applicator in which one syringe was loaded with a mixer of fibrinogen solution, chitin gel, and tGNPs; the other syringe was loaded with a mixture of thrombin solution, chitin gel, and tGNPs. Both these mixtures were injected together. In situ gel formed within a minute and exhibited excellent tissue adhesive property. tGNPsCH-FB gel was found to be cyto-compatible against human umbilical vein endothelial cells (HUVECs). Sustained release of tigecycline from tGNPsCH-FB gel was found to occur over 21 days. In vitro antibacterial activity of tGNPsCH-FB gel was tested against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli (E. coli), and their clinical isolates. Furthermore, in vivo hemostatic potential of tGNPsCH-FB gel was evaluated in deep organ injuries created in Sprague–Dawley rats. The developed gel exhibited rapid blood clotting potential by achieving hemostasis within 154 and 84 s under femoral artery (pressured) and liver (oozing) bleeding conditions. Hence, these findings exhibit the potential application of the developed tGNPsCH-FB gel to adhere at surgical site for controlling bleeding and prevent bacterial infection after cardiac surgery.
    Keywords antibacterial properties ; antibiotic resistance ; applicators ; bacterial infections ; bioadhesives ; blood coagulation ; chitin ; Escherichia coli ; fibrinogen ; gelatin ; hemorrhage ; hemostasis ; human umbilical vein endothelial cells ; liver ; mediastinum ; methicillin ; methicillin-resistant Staphylococcus aureus ; nanocomposites ; nanoparticles ; patients ; rats ; surgery ; thrombin ; tigecycline
    Language English
    Dates of publication 2018-0420
    Size p. 7826-7840.
    Publishing place American Chemical Society
    Document type Article
    ISSN 2168-0485
    DOI 10.1021/acssuschemeng.8b00915
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  6. Article: Mechanistic understanding of Phenyllactic acid mediated inhibition of quorum sensing and biofilm development in Pseudomonas aeruginosa

    Chatterjee, Maitrayee / Sharon D’Morris / Vinod Paul / Sruthi Warrier / Anil Kumar Vasudevan / Muralidharan Vanuopadath / Sudarslal Sadasivan Nair / Bindhu Paul-Prasanth / C. Gopi Mohan / Raja Biswas

    Applied microbiology and biotechnology. 2017 Nov., v. 101, no. 22

    2017  

    Abstract: Pseudomonas aeruginosa depends on its quorum sensing (QS) system for its virulence factors’ production and biofilm formation. Biofilms of P. aeruginosa on the surface of indwelling catheters are often resistant to antibiotic therapy. Alternative ... ...

    Abstract Pseudomonas aeruginosa depends on its quorum sensing (QS) system for its virulence factors’ production and biofilm formation. Biofilms of P. aeruginosa on the surface of indwelling catheters are often resistant to antibiotic therapy. Alternative approaches that employ QS inhibitors alone or in combination with antibiotics are being developed to tackle P. aeruginosa infections. Here, we have studied the mechanism of action of 3-Phenyllactic acid (PLA), a QS inhibitory compound produced by Lactobacillus species, against P. aeruginosa PAO1. Our study revealed that PLA inhibited the expression of virulence factors such as pyocyanin, protease, and rhamnolipids that are involved in the biofilm formation of P. aeruginosa PAO1. Swarming motility, another important criterion for biofilm formation of P. aeruginosa PAO1, was also inhibited by PLA. Gene expression, mass spectrometric, functional complementation assays, and in silico data indicated that the quorum quenching and biofilm inhibitory activities of PLA are attributed to its ability to interact with P. aeruginosa QS receptors. PLA antagonistically binds to QS receptors RhlR and PqsR with a higher affinity than its cognate ligands N-butyryl-L-homoserine lactone (C₄–HSL) and 2-heptyl-3,4-dihydroxyquinoline (PQS; Pseudomonas quinolone signal). Using an in vivo intraperitoneal catheter-associated medaka fish infection model, we proved that PLA inhibited the initial attachment of P. aeruginosa PAO1 on implanted catheter tubes. Our in vitro and in vivo results revealed the potential of PLA as anti-biofilm compound against P. aeruginosa.
    Keywords Lactobacillus ; Oryzias latipes ; Pseudomonas aeruginosa ; antibiotics ; bacterial motility ; biofilm ; catheters ; fish diseases ; gene expression ; ligands ; mass spectrometry ; mechanism of action ; models ; phenyllactic acid ; proteinases ; pyocyanin ; quinolones ; quorum sensing ; receptors ; rhamnolipids ; therapeutics ; virulence
    Language English
    Dates of publication 2017-11
    Size p. 8223-8236.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-017-8546-4
    Database NAL-Catalogue (AGRICOLA)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 79/727: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  7. Article: Amidase, a cell wall hydrolase, elicits protective immunity against Staphylococcus aureus and S. epidermidis

    Nair, Nisha / Anil Kumar Vasudevan / Lalitha Biswas / Maneesha K. Suresh / Raja Biswas / Reshmi Peethambaran / Sukhithasri Vijayrajratnam / Vivek Vinod

    International journal of biological macromolecules. 2015 June, v. 77

    2015  

    Abstract: The morbidity and the mortality associated with Staphylococcus aureus and S. epidermidis infections have greatly increased due to the rapid emergence of highly virulent and antibiotic resistant strains. Development of a vaccine-based therapy is greatly ... ...

    Abstract The morbidity and the mortality associated with Staphylococcus aureus and S. epidermidis infections have greatly increased due to the rapid emergence of highly virulent and antibiotic resistant strains. Development of a vaccine-based therapy is greatly desired. However, no staphylococcal vaccine is available till date. In this study, we have identified Major amidase (Atl-AM) as a prime candidate for future vaccine design against these pathogens. Atl-AM is a multi-functional non-covalently cell wall associated protein which is involved in staphylococcal cell separation after cell division, host extracellular matrix adhesion and biofilm formation. Atl-AM is present on the surface of diverse S. aureus and S. epidermidis strains. When used in combination with Freund's adjuvant, Atl-AM generated a mixed Th1 and Th2 mediated immune response which is skewed more toward Th1; and showed increased production of opsonophagocytic IgG2a and IgG2b antibodies. Significant protective immune response was observed when vaccinated mice were challenged with S. aureus or S. epidermidis. Vaccination prevented the systemic dissemination of both organisms. Our results demonstrate the remarkable efficacy of Atl-AM as a vaccine candidate against both of these pathogens.
    Keywords adhesion ; adjuvants ; amidase ; antibiotic resistance ; antibodies ; biofilm ; cell division ; cell walls ; extracellular matrix ; immune response ; immunoglobulin G ; mice ; morbidity ; mortality ; pathogens ; Staphylococcus aureus ; Staphylococcus epidermidis ; vaccination ; vaccine development ; vaccines ; virulence
    Language English
    Dates of publication 2015-06
    Size p. 314-321.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2015.03.047
    Database NAL-Catalogue (AGRICOLA)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 2374: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top