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Article ; Online: The influence of habitual salt intake on bone remodelling in young healthy people

Erna Davidović-Cvetko / Anita Matić / Jasminka Milas-Ahić / Ines Drenjančević

Zdravniški Vestnik, Vol 90, Iss 7-8, Pp 380-

2021 Volume 389

Abstract: Introduction: Sodium alters calcium metabolism by increasing calcium excretion, thus possibly influencing bone metabolism. The hypothesis of the present study is that amount of dietary sodium intake affects the bone remodelling. This study aimed to ... ...

Abstract	Introduction: Sodium alters calcium metabolism by increasing calcium excretion, thus possibly influencing bone metabolism. The hypothesis of the present study is that amount of dietary sodium intake affects the bone remodelling. This study aimed to assess whether a habitual intake of sodium has an effect on peak bone mass and biochemical indicators of bone metabolism. Subjects and Methods: In a cross-sectional study that involved 41 young men and women, six biochemical markers were assessed from blood samples using ELISA: osteocalcin, C-terminal procollagen type I peptide, receptor activator kappa B ligand, pyridinoline, parathyroid hormone, and osteoprotegerin, while bone mineral density (BMD) and bone mineral content (BMC) were measured by dual x-ray absorptiometry. Subjects were divided into two groups according to habitual sodium intake (low-Na and high-Na group) assessed by questionnaire. Results: No difference was found between groups of low and high Na intake in BMD and BMC, or in biochemical markers of bone metabolism. Since the groups differed in Ca intake, energy and vitamin D, adjustments were made for those cofounders. Regression analysis showed that only the dietary intake of vitamin D was associated with dual femur BMD and BMC, and no correlation was found between bone remodelling indicators and Na intake after adjustment for vitamin D intake. Conclusion: The present results could not confirm that habitual sodium intake above recommended levels affects bone remodelling processes or decreases bone mineral density in young healthy people if combined with adequate calcium intake.
Keywords	sodium intake ; habitual diet ; bone density ; bone mineral content ; bone remodeling ; Medicine ; R
Subject code	616
Language	English
Publishing date	2021-08-01T00:00:00Z
Publisher	Slovenian Medical Association
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Anthropometric and Biochemical Parameters in Relation to Dietary Habits as Early Indicator of Cardiovascular Impairment in Young Adult Cohort

Nikolina Kolobarić / Maja Gradinjan Centner / Petar Šušnjara / Anita Matić / Ines Drenjančević

International Journal of Environmental Research and Public Health, Vol 17, Iss 9208, p

2020 Volume 9208

Abstract: Adjusted dietary assessment questionnaire was used to determine dietary habits of medical students which were related to biochemical and anthropometric markers of studied cohort. Thirty-seven young and healthy volunteers aged 19–28 years old entered the ... ...

Abstract	Adjusted dietary assessment questionnaire was used to determine dietary habits of medical students which were related to biochemical and anthropometric markers of studied cohort. Thirty-seven young and healthy volunteers aged 19–28 years old entered the protocol and were divided according to sex and according to residence. Subjects were given questionnaires for tracking food/beverage consumption. Venous blood samples were taken after overnight fast ( n = 32). Nutrient status and energy consumption were determined and analyzed. Study population had normal weight and body mass index (BMI). Biochemical characteristics were within normal reference range, while some participants had lipid profile disbalance. Men had significantly higher BMI than women. Average BMI was significantly higher in participants with elevated cholesterol levels compared to participants with normal cholesterol levels. Majority of participants consumed less than five meals per day with no major differences between students according to residence and sex. Men had significantly higher protein intake and consumed at least four meals daily compared to woman who had three or less meals daily with no differences in intake according to residence. Students with normal lipid profile consumed more carbohydrates than students with increased cholesterol. Results suggest that students with bad dietary habits have potentially higher risk for future cardiovascular problems, even before the onset of adverse effects.
Keywords	diet ; body mass index ; cholesterol levels ; Medicine ; R
Subject code	796 ; 150
Language	English
Publishing date	2020-12-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Effect of Low Selenium Diet on Glutathione Peroxidase 3 Concentration in Male Sprague-Dawley Rats’ Serum

Nikolina Kolobaric (ex. Bilic-Dujmusic) / Ana Stupin / Petar Šušnjara / Lidija Barić / Anita Matic

Southeastern European Medical Journal, Vol 3, Iss 1, Pp 50-

2019 Volume 59

Abstract: Aim: Determination of antioxidative enzyme glutathione peroxidase 3 (GPx3) serum concentrations after consumption of food which contains different concentrations of selenium (Se). Research subjects and methods: Four-week-old Sprague Dawley rats consumed ... ...

Abstract	Aim: Determination of antioxidative enzyme glutathione peroxidase 3 (GPx3) serum concentrations after consumption of food which contains different concentrations of selenium (Se). Research subjects and methods: Four-week-old Sprague Dawley rats consumed food containing different concentrations of Se (food Divan) over a period of 10 weeks. The animals were divided into two groups: 1) normal Se (0.363 mg/kg Se) and 2) low Se (0.030 mg/kg Se). Each animal was weighed at the end of protocol, and serum samples were collected for determining GPx3 concentrations. All experimental procedures were in compliance with the European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes and approved by the Ethics Committee of the Faculty of Medicine in Osijek and the Ministry of Agriculture of the Republic of Croatia. Results: Different concentrations of Se in food did not cause a change in body weight. Food containing the recommended intake of Se according to the guidelines of the World Health Organization significantly increased GPx3 enzyme concentration (13.96±0.42 mg/ml) when compared to low selective Se (12.04 ± 0.33 mg/ml, p = 0.002). Conclusion: Serum concentration of the antioxidant enzyme GPx3 depends on the concentration of Se in food. It is shown that, in comparison with food with low Se levels, food containing a normal concentration of Se is enriched with the antioxidant GPx3 which, according to numerous studies, has a protective role in the human body.
Keywords	antioxidative enzymes ; glutathione peroxidase 3 ; Se ; Sprague Dawley rats ; Medicine ; R
Subject code	590 ; 630
Language	English
Publishing date	2019-05-01T00:00:00Z
Publisher	Faculty of Medicine Osijek
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Enhanced Antioxidative Defense by Vitamins C and E Consumption Prevents 7-Day High-Salt Diet-Induced Microvascular Endothelial Function Impairment in Young Healthy Individuals

Lidija Barić / Ines Drenjančević / Martina Mihalj / Anita Matić / Marko Stupin / Luka Kolar / Zrinka Mihaljević / Ines Mrakovčić-Šutić / Vatroslav Šerić / Ana Stupin

Journal of Clinical Medicine, Vol 9, Iss 3, p

2020 Volume 843

Abstract: This study aimed to examine whether the oral supplementation of vitamins C and E during a seven-day high salt diet (HS; ~14 g salt/day) prevents microvascular endothelial function impairment and changes oxidative status caused by HS diet in 51 (26 women ... ...

Abstract	This study aimed to examine whether the oral supplementation of vitamins C and E during a seven-day high salt diet (HS; ~14 g salt/day) prevents microvascular endothelial function impairment and changes oxidative status caused by HS diet in 51 (26 women and 25 men) young healthy individuals. Laser Doppler flowmetry measurements demonstrated that skin post-occlusive reactive hyperemia (PORH), and acetylcholine-induced dilation (AChID) were significantly impaired in the HS group, but not in HS+C+E group, while sodium nitroprusside-induced dilation remained unaffected by treatments. Serum oxidative stress markers: Thiobarbituric acid reactive substances (TBARS), 8-iso prostaglandin-F2α, and leukocytes’ intracellular hydrogen peroxide (H 2 O 2 ) production were significantly increased, while ferric-reducing ability of plasma (FRAP) and catalase concentrations were decreased in the HS group. All these parameters remained unaffected by vitamins supplementation. Matrix metalloproteinase 9, antioxidant enzymes Cu/Zn SOD and glutathione peroxidase 1, and leukocytes’ intracellular superoxide production remained unchanged after the protocols in both HS and HS+C+E groups. Importantly, multiple regression analysis revealed that FRAP was the most powerful predictor of AChID, while PORH was strongly predicted by both FRAP and renin-angiotensin system activity. Hereby, we demonstrated that oxidative dis-balance has the pivotal role in HS diet-induced impairment of endothelial and microvascular function in healthy individuals which could be prevented by antioxidative vitamins consumption.
Keywords	high-salt diet ; microcirculation ; endothelium ; oxidative stress ; antioxidative defense ; vitamin c ; vitamin e ; renin-angiotensin system ; Medicine ; R
Subject code	610
Language	English
Publishing date	2020-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Anti-Inflammatory Potential of n-3 Polyunsaturated Fatty Acids Enriched Hen Eggs Consumption in Improving Microvascular Endothelial Function of Healthy Individuals—Clinical Trial

Ana Stupin / Martina Mihalj / Nikolina Kolobarić / Petar Šušnjara / Luka Kolar / Zrinka Mihaljević / Anita Matić / Marko Stupin / Ivana Jukić / Zlata Kralik / Manuela Grčević / Gordana Kralik / Vatroslav Šerić / Ines Drenjančević

International Journal of Molecular Sciences, Vol 21, Iss 4149, p

2020 Volume 4149

Abstract: The effects of consumption of n-3 polyunsaturated fatty acids (n-3 PUFAs) enriched hen eggs on endothelium-dependent and endothelium-independent vasodilation in microcirculation, and on endothelial activation and inflammation were determined in young ... ...

Abstract	The effects of consumption of n-3 polyunsaturated fatty acids (n-3 PUFAs) enriched hen eggs on endothelium-dependent and endothelium-independent vasodilation in microcirculation, and on endothelial activation and inflammation were determined in young healthy individuals. Control group ( N = 21) ate three regular hen eggs/daily (249 mg n-3 PUFAs/day), and n-3 PUFAs group ( N = 19) ate three n-3 PUFAs enriched hen eggs/daily (1053 g n-3 PUFAs/day) for 3 weeks. Skin microvascular blood flow in response to iontophoresis of acetylcholine (AChID; endothelium-dependent) and sodium nitroprusside (SNPID; endothelium-independent) was assessed by laser Doppler flowmetry. Blood pressure (BP), body composition, body fluid status, serum lipid and free fatty acids profile, and inflammatory and endothelial activation markers were measured before and after respective dietary protocol. Results: Serum n-3 PUFAs concentration significantly increased, AChID significantly improved, and SNPID remained unchanged in n-3 PUFAs group, while none was changed in Control group. Interferon-γ (pro-inflammatory) significantly decreased and interleukin-10 (anti-inflammatory) significantly increased in n-3 PUFAs. BP, fat free mass, and total body water significantly decreased, while fat mass, interleukin-17A (pro-inflammatory), interleukin-10 and vascular endothelial growth factor A significantly increased in the Control group. Other measured parameters remained unchanged in both groups. Favorable anti-inflammatory properties of n-3 PUFAs consumption potentially contribute to the improvement of microvascular endothelium-dependent vasodilation in healthy individuals.
Keywords	n-3 polyunsaturated fatty acids ; omega-3 polyunsaturated fatty acids ; endothelium ; microvascular ; inflammation ; laser Doppler flowmetry ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	571
Language	English
Publishing date	2020-06-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Arachidonic Acid Metabolites of CYP450 Enzymes and HIF-1α Modulate Endothelium-Dependent Vasorelaxation in Sprague-Dawley Rats under Acute and Intermittent Hyperbaric Oxygenation

Zrinka Mihaljević / Anita Matić / Ana Stupin / Ruža Frkanec / Branka Tavčar / Vanja Kelava / Ivana Tartaro Bujak / Nikolina Kolobarić / Aleksandar Kibel / Ines Drenjančević

International Journal of Molecular Sciences, Vol 21, Iss 6353, p

2020 Volume 6353

Abstract: Acetylcholine-induced vasorelaxation (AChIR) and responses to reduced pO 2 (hypoxia-induced relaxation (HIR), 0% O 2 ) were assessed in vitro in aortic rings of healthy male Sprague-Dawley rats (N = 252) under hyperbaric (HBO 2 ) protocols. The studied ... ...

Abstract	Acetylcholine-induced vasorelaxation (AChIR) and responses to reduced pO 2 (hypoxia-induced relaxation (HIR), 0% O 2 ) were assessed in vitro in aortic rings of healthy male Sprague-Dawley rats (N = 252) under hyperbaric (HBO 2 ) protocols. The studied groups consisted of the CTRL group (untreated); the A-HBO 2 group (single HBO 2 120 min of 100% O 2 at 2.0 bars); the 24H-HBO 2 group (examined 24 h after single exposure) and the 4D-HBO 2 group (four consecutive days of single HBO 2 ). AChIR, sensitivity to ACh and iNOS expression were decreased in the A-HBO 2 group. HIR was prostanoid- and epoxyeicosatrienoic acid (EET)-mediated. HIF-1α expression was increased in the 24H-HBO 2 and 4D-HBO 2 groups. LW6 (HIF-1α inhibitor) decreased HIR in the 24H-HBO 2 group. HBO 2 affected the expression of COX-1 and COX-2. CYP2c11 expression was elevated in the 24H-HBO 2 and 4D-HBO 2 groups. Concentrations of arachidonic acid (AA) metabolites 14(15)-DiHET, 11(12)-DiHET and 8(9)-DiHET were increased in A-HBO 2 and 24H-HBO 2. An increased concentration of 8(9)-EET was observed in the A-HBO 2 and 24h-HBO 2 groups vs. the CTRL and 4D-HBO 2 groups, and an increased concentration of 5(6)-DiHET was observed in the 24H-HBO 2 group vs. the 4D-HBO 2 group. The 20-HETE concentration was increased in the A-HBO 2 group. All were determined by LC-MS/MS of the aorta. The results show that AChIR in all groups is mostly NO-dependent. HIR is undoubtedly mediated by the CYP450 enzymes’ metabolites of AA, whereas HIF-1α contributes to restored HIR. Vasoconstrictor metabolites of CYP450 enzymes contribute to attenuated AChIR and HIR in A-HBO 2 .
Keywords	acetylcholine ; aortic rings ; epoxyeicosatrienoic acids ; hyperbaric oxygenation ; hypoxia ; HIF-1α ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	290
Language	English
Publishing date	2020-09-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Effects of n-3 Polyunsaturated Fatty Acid-Enriched Hen Egg Consumption on the Inflammatory Biomarkers and Microvascular Function in Patients with Acute and Chronic Coronary Syndrome—A Randomized Study

Željka Breškić Ćurić / Ana Marija Masle / Aleksandar Kibel / Kristina Selthofer-Relatić / Ana Stupin / Zrinka Mihaljević / Ivana Jukić / Marko Stupin / Anita Matić / Nataša Kozina / Petar Šušnjara / Brankica Juranić / Nikolina Kolobarić / Vatroslav Šerić / Ines Drenjančević

Biology, Vol 10, Iss 774, p

2021 Volume 774

Abstract: This study aimed to test the effect of n-3 polyunsaturated fatty acid (PUFA)-enriched hen egg consumption on serum lipid and free fatty acid profiles, inflammatory and oxidative stress biomarkers, and microvascular reactivity in patients with coronary ... ...

Abstract	This study aimed to test the effect of n-3 polyunsaturated fatty acid (PUFA)-enriched hen egg consumption on serum lipid and free fatty acid profiles, inflammatory and oxidative stress biomarkers, and microvascular reactivity in patients with coronary artery disease (CAD). Forty CAD patients participated in this study. Of those, 20 patients had acute CAD (Ac-CAD), and 20 patients had chronic CAD (Ch-CAD). The control group (N = 20) consumed three regular hen eggs/daily (249 mg n-3 PUFAs/day), and the n-3 PUFAs group (N = 20) consumed three n-3 PUFA-enriched hen eggs/daily (1053 g n-3 PUFAs/day) for 3 weeks. Serum n-3 PUFA concentration significantly increased (in all CAD patients), while LDL cholesterol and IL-6 (in Ac-CAD patients), and hsCRP and IL-1a (in all CAD patients) significantly decreased in the n-3 PUFAs group. Glutathione peroxidase (GPx) activity significantly decreased, and forearm skin microvascular reactivity in response to vascular occlusion (postocclusive reactive hyperemia (PORH)) remained unchanged in both the n-3 PUFAs and control groups in total CAD, Ac-CAD, and Ch-CAD patients. Potentially, n-3 PUFA-enriched hen eggs can change the free fatty acid profile to a more favorable lower n6/n3 ratio, and to exhibit mild anti-inflammatory effects but not to affect microvascular reactivity in CAD patients.
Keywords	n-3 polyunsaturated fatty acids ; functional food ; coronary artery disease ; inflammation ; oxidative stress ; microcirculation ; Biology (General) ; QH301-705.5
Subject code	610
Language	English
Publishing date	2021-08-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3 −/− / C57BL/6N Knockout and WT (C57BL/6N) Control Mice

Nataša Kozina / Zrinka Mihaljević / Mirela Baus Lončar / Martina Mihalj / Mihael Mišir / Marina Dobrivojević Radmilović / Helena Justić / Srećko Gajović / Kate Šešelja / Iva Bazina / Anita Horvatić / Anita Matić / Nikola Bijelić / Edi Rođak / Ivana Jukić / Ines Drenjančević

International Journal of Molecular Sciences, Vol 20, Iss 20, p

2019 Volume 5188

Abstract: High salt (HS) dietary intake leads to impaired vascular endothelium-dependent responses to various physiological stimuli, some of which are mediated by arachidonic acid (AA) metabolites. Transgenic Tff3 −/− gene knockout mice ( Tff3 −/− / C57BL/6N) have ...

Abstract	High salt (HS) dietary intake leads to impaired vascular endothelium-dependent responses to various physiological stimuli, some of which are mediated by arachidonic acid (AA) metabolites. Transgenic Tff3 −/− gene knockout mice ( Tff3 −/− / C57BL/6N) have changes in lipid metabolism which may affect vascular function and outcomes of stroke. We aimed to study the effects of one week of HS diet (4% NaCl) on vascular function and stroke induced by transient occlusion of middle cerebral artery in Tff3 −/− and wild type (WT/C57BL/6N) mice. Flow-induced dilation (FID) of carotid artery was reduced in WT-HS mice, but not affected in Tff3 −/− - HS mice. Nitric oxide (NO) mediated FID. NO production was decreased with HS diet. On the contrary, acetylcholine-induced dilation was significantly decreased in Tff3 −/− mice on both diets and WT-HS mice. HS intake and Tff3 gene depletion affected the structural components of the vessels. Proteomic analysis revealed a significant effect of Tff3 gene deficiency on HS diet-induced changes in neuronal structural proteins and acute innate immune response proteins’ expression and Tff3 depletion, but HS diet did not increase the stroke volume, which is related to proteome modification and upregulation of genes involved mainly in cellular antioxidative defense. In conclusion, Tff3 depletion seems to partially impair vascular function and worsen the outcomes of stroke, which is moderately affected by HS diet.
Keywords	tff3 gene ; flow-induced dilation ; high salt diet ; stroke ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Language	English
Publishing date	2019-10-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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