Article ; Online: Point-of-care impedimetric aptasensor to detect the luteinizing hormone.
2024 Volume 191, Issue 2, Page(s) 115
Abstract: Luteinizing hormone (LH) is a useful biomarker for identifying ovulation events in the cows to predict the time of ovulation to achieve a high success rate of conception following artificial insemination. Although antibody-based radioimmunoassay and ... ...
| Abstract | Luteinizing hormone (LH) is a useful biomarker for identifying ovulation events in the cows to predict the time of ovulation to achieve a high success rate of conception following artificial insemination. Although antibody-based radioimmunoassay and enzyme-linked immunosorbent assay are being used for LH measurement, these techniques are expensive, time-consuming, and require expertise and sophisticated laboratory facilities. So, there is a need for a field-applicable, affordable, easy-to-use method for LH detection. For developing such a specific, quantitative, and inexpensive system, an aptamer-based smartphone-enabled aptasensor has been investigated. The aptamer was used instead of the antibody as a biorecognition element due to its comparative stability at ambient temperature, ease of synthesis, and cost-effectiveness. Electrochemical impedance spectroscopy has been used to obtain label-free detection of LH within 20 min in ~ 20 μL sample volume. The screen-printed gold electrode is compatible with a smartphone-enabled miniaturized device (Sensit Smart; Palmsens BV, The Netherlands) and was fabricated with the aptamer to detect LH in biological fluids (limit of detection 0.80 and 0.61 ng/mL in buffer and undiluted/unprocessed serum, respectively, with the dynamic range of detection of 0.01 to 50 ng/mL). All the data were obtained in the 10 kHz to 0.10 Hz frequency range at a bias potential of 0.30 V with an alternating potential of 10 mV. The clinical relevance of the sensor was evaluated in 10 serum samples collected from dairy animals which established a high correlation with standard LH-ELISA (κ > 0.87). The aptasensor can be stored at room temperature for 30 days without any significant loss in electrochemical sensing ability. |
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| MeSH term(s) | Animals ; Cattle ; Luteinizing Hormone ; Point-of-Care Systems ; Aptamers, Nucleotide/chemistry ; Biosensing Techniques/methods ; Electrochemical Techniques/methods |
| Chemical Substances | Luteinizing Hormone (9002-67-9) ; Aptamers, Nucleotide |
| Language | English |
| Publishing date | 2024-01-30 |
| Publishing country | Austria |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 89-9 |
| ISSN | 1436-5073 ; 0026-3672 |
| ISSN (online) | 1436-5073 |
| ISSN | 0026-3672 |
| DOI | 10.1007/s00604-024-06191-w |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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