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Article ; Online: Tumour generated exosomal miRNAs: A major player in tumour angiogenesis.

Kumar, V B Sameer / Anjali, K

Biochimica et biophysica acta. Molecular basis of disease

2022 Volume 1868, Issue 6, Page(s) 166383

Abstract: One of the major reasons for the non-effectiveness of anticancer therapeutics designed to target tumour angiogenesis is the involvement of multiple layers of cellular communication that exists within the tumour microenvironment. Any attempt to block one ... ...

Abstract	One of the major reasons for the non-effectiveness of anticancer therapeutics designed to target tumour angiogenesis is the involvement of multiple layers of cellular communication that exists within the tumour microenvironment. Any attempt to block one signaling pathway by targeting one of its components led to the activation of alternate pathway that overcome this block rendering the therapy ineffective. During the previous decades most of the research was focused on manipulating signaling pathways initiated by soluble factors and their intermediates. More recently the work from our lab and many advanced labs all over the globe have brought into light, the significance of signaling events mediated by exosomes in regulation of tumour progression and angiogenesis. In this mini review we intend to collate the information available from several groups including ours related to the role of exosomal cargo especially exosomal miRNAs in modulating tumour angiogenesis.
MeSH term(s)	Exosomes/metabolism ; Humans ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Neoplasms/pathology ; Neovascularization, Pathologic/pathology ; Tumor Microenvironment/genetics
Chemical Substances	MicroRNAs
Language	English
Publishing date	2022-03-12
Publishing country	Netherlands
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	60-7
ISSN	1879-260X ; 1879-2596 ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
ISSN (online)	1879-260X ; 1879-2596 ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650
ISSN	0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
DOI	10.1016/j.bbadis.2022.166383
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Article ; Online: Exploring the last 50 years of Indian research on the impact of coal mining using bibliometric analysis with an overview of water-related impacts

Anjali, Kumari / Remesan, Renji

Environ Sci Pollut Res. 2023 Feb., v. 30, no. 6 p.16449-16463

2023

Abstract: This paper throws light on the bibliometric review of the impact of coal mining in India over the past 50 years, emphasizing environmental, especially water-related impacts. The data were refined from the Web of Science database and analyzed in a ... ...

Abstract	This paper throws light on the bibliometric review of the impact of coal mining in India over the past 50 years, emphasizing environmental, especially water-related impacts. The data were refined from the Web of Science database and analyzed in a bibliometric map visualization software tool, VOSviewer, to grasp the research focus, status quo and analyze the trend and direction of the work being carried out in this area. The methodology was covered in three phases: search and document selection, software and data extraction, and analysis of results and trends. The study results indicated that (i) the publication has increased in the past two decades (2001–2021) with a steep increase in the period from 2010 to 2021 with 74.68% article types documents and a mere 7.74% review documents. (ii) In India, the significant contribution is made by the Indian Institute of Technology (Indian School of Mines), Dhanbad with Department of Science and Technology as a primary funding agency. (iii) The bibliometric map of co-occurrence of author keywords showed that keywords relating to the “pollution” (connected to air, water) from “Jharia coalfield” have highest occurrences in the relevant published works of literature and topics like “reclamation,” “mine spoil,” and application of approaches like “remote sensing and GIS” have lower linkage strengths in general. (iv) The result of the co-citation network study has marked the most significant authors and the highly cited sources of the database revealing Ghose M.K. and Singh A.K. as among the most cited authors with citations more than 150 in the field of our interest. (v) The trend of publication in the research area of Water Resources showed a significant increase after 2015. The keyword occurrence map reveals that water quality studies have been extensively studied, but quantifications of the coal mining-induced changes in water regimes at river basin scales are absent.
Keywords	bibliometric analysis ; coal ; computer software ; databases ; mine spoil ; water quality ; watersheds ; India
Language	English
Dates of publication	2023-02
Size	p. 16449-16463.
Publishing place	Springer Berlin Heidelberg
Document type	Article ; Online
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-022-23381-2
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Article: KCNQ2/3 regulates efferent mediated slow excitation of vestibular afferents in mammals.

Sinha, Anjali K / Lee, Choongheon / Holt, Joseph C

bioRxiv : the preprint server for biology

2024

Abstract: Primary vestibular afferents transmit information from hair cells about head position and movement to the CNS, which is critical for maintaining balance, gaze stability and spatial navigation. The CNS, in turn, modulates hair cells and afferents via the ... ...

Abstract	Primary vestibular afferents transmit information from hair cells about head position and movement to the CNS, which is critical for maintaining balance, gaze stability and spatial navigation. The CNS, in turn, modulates hair cells and afferents via the efferent vestibular system (EVS) and its activation of several cholinergic signaling mechanisms. Electrical stimulation of EVS neurons gives rise to three kinetically- and mechanistically-distinct afferent responses including a slow excitation, a fast excitation, and a fast inhibition. EVS-mediated slow excitation is attributed to odd-numbered muscarinic acetylcholine receptors (mAChRs) on the afferent whose activation leads to the closure of a potassium conductance and increased afferent discharge. Likely effector candidates include low-threshold, voltage-gated potassium channels belonging to the KCNQ (Kv7.X) family, which are involved in neuronal excitability across the nervous system and are subject to mAChR modulation. Specifically, KCNQ2/3 heteromeric channels may be the molecular correlates for the M-current, a potassium current that is blocked following the activation of odd-numbered mAChRs. To this end, multiple members of the KCNQ channel family, including KCNQ2 and KCNQ3, are localized to several microdomains within vestibular afferent endings, where they influence afferent excitability and could be targeted by EVS neurons. Additionally, the relative expression of KCNQ subunits appears to vary across the sensory epithelia and among different afferent types. However, it is unclear which KCNQ channel subunits are targeted by mAChR activation and whether that also varies among different afferent classes. Here we show that EVS-mediated slow excitation is blocked and enhanced by the non-selective KCNQ channel blocker XE991 and opener retigabine, respectively. Using KCNQ subunit-selective drugs, we observed that a KCNQ2 blocker blocks the slow response in irregular afferents, while a KCNQ2/3 opener enhances slow responses in regular afferents. The KCNQ2 blockers did not appear to affect resting afferent discharge rates, while KCNQ2/3 or KCNQ2/4 openers decreased afferent excitability. Here, we show pharmacological evidence that KCNQ2/3 subunits are likely targeted by mAChR activation in mammalian vestibular afferents. Additionally, we show that KCNQ3 KO mice have altered resting discharge rate as well as EVS-mediated slow response. These data together suggest that KCNQ channels play a role in slow response and discharge rate of vestibular afferents, which can be modulated by EVS in mammals.
Language	English
Publishing date	2024-01-01
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.12.30.573731
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Article ; Online: Exploring the last 50 years of Indian research on the impact of coal mining using bibliometric analysis with an overview of water-related impacts.

Anjali, Kumari / Remesan, Renji

Environmental science and pollution research international

2022 Volume 30, Issue 6, Page(s) 16449–16463

Abstract	This paper throws light on the bibliometric review of the impact of coal mining in India over the past 50 years, emphasizing environmental, especially water-related impacts. The data were refined from the Web of Science database and analyzed in a bibliometric map visualization software tool, VOSviewer, to grasp the research focus, status quo and analyze the trend and direction of the work being carried out in this area. The methodology was covered in three phases: search and document selection, software and data extraction, and analysis of results and trends. The study results indicated that (i) the publication has increased in the past two decades (2001-2021) with a steep increase in the period from 2010 to 2021 with 74.68% article types documents and a mere 7.74% review documents. (ii) In India, the significant contribution is made by the Indian Institute of Technology (Indian School of Mines), Dhanbad with Department of Science and Technology as a primary funding agency. (iii) The bibliometric map of co-occurrence of author keywords showed that keywords relating to the "pollution" (connected to air, water) from "Jharia coalfield" have highest occurrences in the relevant published works of literature and topics like "reclamation," "mine spoil," and application of approaches like "remote sensing and GIS" have lower linkage strengths in general. (iv) The result of the co-citation network study has marked the most significant authors and the highly cited sources of the database revealing Ghose M.K. and Singh A.K. as among the most cited authors with citations more than 150 in the field of our interest. (v) The trend of publication in the research area of Water Resources showed a significant increase after 2015. The keyword occurrence map reveals that water quality studies have been extensively studied, but quantifications of the coal mining-induced changes in water regimes at river basin scales are absent.
MeSH term(s)	Bibliometrics ; Coal Mining ; Databases, Factual ; Environment ; Software
Language	English
Publishing date	2022-10-03
Publishing country	Germany
Document type	Journal Article ; Review
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-022-23381-2
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Article: Evaluation of microbial contamination in removable dental prosthesis at different time of usage.

Singh, Tulika / Bhojaraju, Nandakishore / Vinod, V / Anjali, K / Shah, Samsuddeen / Kumar, Naveen

Journal of oral and maxillofacial pathology : JOMFP

2023 Volume 27, Issue 2, Page(s) 333–339

Abstract: Aim: The current study evaluates and compares the percentage distribution of different microorganisms according to their strains and occurrence among the three studied groups.: Method and methodology: Sample of 30 removable dental prosthesis wearing ... ...

Abstract	Aim: The current study evaluates and compares the percentage distribution of different microorganisms according to their strains and occurrence among the three studied groups. Method and methodology: Sample of 30 removable dental prosthesis wearing patients was selected: wearing either complete dentures or partial dentures and without any significant medical history or on prescription medication for the past 3-6 months. Samples were obtained in three subcategories based on the duration of prostheses worn by the patient. A sterile swab made up of cotton moistened with phosphate buffer saline (PBS) was scrubbed on the dental prosthesis at the fitting surfaces and the denture-bearing area of the oral cavity. Within two hours, the collected swab sample was infused in the sterile tube containing 1 ml of 0.84% PBS solution maintained at pH 7-7.2 and sent for microbiological analysis. The samples were then inoculated into different medias. Microbial growth was checked after incubating the culture plates for 48 h at 37°C. Microorganisms were recognized and counted by calibrated colony counter. Gram's stain was used to stain the colony smear and biochemical tests such as coagulase, catalase, oxidase, sugar fermentation with acid and gas production (triple sugar iron), methyl red test, test for indole production, hydrogen sulphide (H Statistical analysis: Descriptive statistics included calculation of means and standard deviation using multivariate analysis. All values were considered statistically significant for a value of Results: Streptococcus species, Coagulase-negative staphylococcus, Staphylococcus aureus, Candida albicans and Klebsiella pneumoniae Conclusion: A progressive increase in the microbial contamination was directly proportional to the duration of removable prosthesis usage.
Language	English
Publishing date	2023-07-13
Publishing country	India
Document type	Journal Article
ZDB-ID	2390999-7
ISSN	1998-393X ; 0973-029X
ISSN (online)	1998-393X
ISSN	0973-029X
DOI	10.4103/jomfp.jomfp_157_23
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Article: Linking satellite-based forest cover change with rainfall and land surface temperature in Kerala, India

Anjali, Kumari / Roshni, Thendiyath

Environment, development and sustainability. 2022 Sept., v. 24, no. 9

2022

Abstract: Forest cover change is having an enduring link with climatic factors. This study was designed to study the impact of forest cover change in the Western Ghats on meteorological parameters like rainfall and land surface temperature (LST) between 2000 and ... ...

Abstract	Forest cover change is having an enduring link with climatic factors. This study was designed to study the impact of forest cover change in the Western Ghats on meteorological parameters like rainfall and land surface temperature (LST) between 2000 and 2019 using Landsat images. Land use land cover (LULC) data were extracted from Landsat images, and rainfall data of 53 stations were obtained from tropical rainfall measuring mission (TRMM) data. Mann–Kendall trend test, a nonparametric test, was adopted to evaluate the long-run trends in rainfall for North East (NE) and South West (SW) monsoon during the selected time period. The test results indicate that 19 stations in the North of Kerala show a positive trend in SW monsoon and 3 stations show a negative trend in NE monsoon. The breakpoint analysis of the rainfall dataset with time and with the elevation from mean sea level (MSL) is performed and the results show a breakpoint in the rainfall for the year 2008 and at 47 m elevation (MSL) from the coast of the Arabian Sea. Forest cover data show that there is a loss of 12.65% in the region for two decades span and also a significant increase in minimum LST from 8.308 to 9.208 °C and increase in maximum LST from 41.51 to 46.29 °C during the selected time period. Forest cover loss could be an important factor responsible for the increase in LST. The research outcomes would help the policymakers in improvising the measures for land management policies including plantation and urbanization.
Keywords	Landsat ; coasts ; data collection ; forests ; land management ; land use and land cover maps ; meteorological data ; monsoon season ; rain ; sea level ; surface temperature ; urbanization ; Arabian Sea ; India
Language	English
Dates of publication	2022-09
Size	p. 11282-11300.
Publishing place	Springer Netherlands
Document type	Article
ZDB-ID	1438730-x
ISSN	1387-585X
ISSN	1387-585X
DOI	10.1007/s10668-021-01908-w
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Article: Elucidating the role of muscarinic acetylcholine receptor (mAChR) signaling in efferent mediated responses of vestibular afferents in mammals.

Sinha, Anjali K / Lee, Choongheon / Holt, Joseph C

bioRxiv : the preprint server for biology

2023

Abstract: The peripheral vestibular system detects head position and movement through activation of vestibular hair cells (HCs) in vestibular end organs. HCs transmit this information to the CNS by way of primary vestibular afferent neurons. The CNS, in turn, ... ...

Abstract	The peripheral vestibular system detects head position and movement through activation of vestibular hair cells (HCs) in vestibular end organs. HCs transmit this information to the CNS by way of primary vestibular afferent neurons. The CNS, in turn, modulates HCs and afferents via the efferent vestibular system (EVS) through activation of cholinergic signaling mechanisms. In mice, we previously demonstrated that activation of muscarinic acetylcholine receptors (mAChRs), during EVS stimulation, gives rise to a slow excitation that takes seconds to peak and tens of seconds to decay back to baseline. This slow excitation is mimicked by muscarine and ablated by the non-selective mAChR blockers scopolamine, atropine, and glycopyrrolate. While five distinct mAChRs (M1-M5) exist, the subtype(s) driving EVS-mediated slow excitation remain unidentified and details on how these mAChRs alter vestibular function is not well understood. The objective of this study is to characterize which mAChR subtypes drive the EVS-mediated slow excitation, and how their activation impacts vestibular physiology and behavior. In C57Bl/6J mice, M3mAChR antagonists were more potent at blocking slow excitation than M1mAChR antagonists, while M2/M4 blockers were ineffective. While unchanged in M2/M4mAChR double KO mice, EVS-mediated slow excitation in M3 mAChR-KO animals were reduced or absent in irregular afferents but appeared unchanged in regular afferents. In agreement, vestibular sensory-evoked potentials (VsEP), known to be predominantly generated from irregular afferents, were significantly less enhanced by mAChR activation in M3mAChR-KO mice compared to controls. Finally, M3mAChR-KO mice display distinct behavioral phenotypes in open field activity, and thermal profiles, and balance beam and forced swim test. M3mAChRs mediate efferent-mediated slow excitation in irregular afferents, while M1mAChRs may drive the same process in regular afferents.
Language	English
Publishing date	2023-08-06
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.07.31.549902
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Article: Analysis of the Effect of Female Genital Tuberculosis on Ovarian Reserve Parameters.

Richa, Sharma / Anjali, Kanhere / Sonal, Jain / Akrati, Jain

Journal of human reproductive sciences

2023 Volume 16, Issue 2, Page(s) 125–131

Abstract: Background: Female genital tuberculosis (FGTB) is a known cause of female infertility. Worldwide incidence is 5%-10% and annual burden in India is around 4%-7%. It is known to cause tubal and endometrial damage. However, the effect on ovarian damage is ... ...

Abstract	Background: Female genital tuberculosis (FGTB) is a known cause of female infertility. Worldwide incidence is 5%-10% and annual burden in India is around 4%-7%. It is known to cause tubal and endometrial damage. However, the effect on ovarian damage is poorly known. The availability of ovarian markers has contributed to an improved understanding of ovarian reserve in FGTB. Aims: The aim of this study was to assess ovarian reserve by measuring anti-Mullerian hormone (AMH) and antral follicle count (AFC) amongst infertile women and analyse the effect of GTB on ovarian reserve parameters. Settings and design: This was a prospective study at a tertiary referral centre for infertility for 18 months. Materials and methods: A total of 133 infertile women who underwent diagnostic hysterolaparoscopy and cartridge-based nucleic acid amplification test testing of an endometrial biopsy were included in the study. AMH and AFC of all the infertile women were assessed and compared between cases with and without FGTB. Statistical analysis used: Independent Results: Fifty-eight (43.6%) cases were diagnosed with FGTB (Group I), and 75 (56.3%) cases were without FGTB (Group II). The mean AMH level 1.88 ng/ml (±1.52) and mean AFC 9.0 (±5.50) were significantly lower ( Conclusion: In women with prolonged infertility and low ovarian reserve, FGTB should be ruled out. Early diagnosis and treatment of GTB may prevent further decline of ovarian reserve and improve the reproductive outcome.
Language	English
Publishing date	2023-06-30
Publishing country	India
Document type	Journal Article
ZDB-ID	2418984-4
ISSN	1998-4766 ; 0974-1208
ISSN (online)	1998-4766
ISSN	0974-1208
DOI	10.4103/jhrs.jhrs_36_23
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Article ; Online: Computational avenues in oral protein and peptide therapeutics.

Pandya, Anjali K / Patravale, Vandana B

Drug discovery today

2021 Volume 26, Issue 6, Page(s) 1510–1520

Abstract: Proteins and peptides are amongst the most sought-after biomolecules because of their exceptional potential to cater to a vast range of diseases. Although widely studied and researched, the oral delivery of these biomolecules remains a challenge. ... ...

Abstract	Proteins and peptides are amongst the most sought-after biomolecules because of their exceptional potential to cater to a vast range of diseases. Although widely studied and researched, the oral delivery of these biomolecules remains a challenge. Alongside formulation strategies, approaches to overcome the inherent barriers for peptide absorption are being designed at the molecular level to establish a sound rationale and to achieve higher bioavailability. Computer-aided drug design (CADD) is a modern in silico approach for developing successful bio-formulations. CADD enables intricate study of the biomolecules in conjunction with their target sites or receptors at the molecular level. Knowledge of the molecular interactions of proteins and peptides makes way for the pre-screening of suitable formulation components and facilitates their delivery.
MeSH term(s)	Administration, Oral ; Animals ; Biological Availability ; Computer Simulation ; Drug Delivery Systems ; Drug Design ; Humans ; Peptides/administration & dosage ; Peptides/pharmacokinetics ; Proteins/administration & dosage ; Proteins/pharmacokinetics
Chemical Substances	Peptides ; Proteins
Language	English
Publishing date	2021-03-05
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1324988-5
ISSN	1878-5832 ; 1359-6446
ISSN (online)	1878-5832
ISSN	1359-6446
DOI	10.1016/j.drudis.2021.03.003
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Article ; Online: Proteins with amino acid repeats constitute a rapidly evolvable and human-specific essentialome

Anjali K. Singh / Ishita Amar / Harikrishnan Ramadasan / Keertana S. Kappagantula / Sreenivas Chavali

Cell Reports, Vol 42, Iss 7, Pp 112811- (2023)

2023

Abstract: Summary: Protein products of essential genes, indispensable for organismal survival, are highly conserved and bring about fundamental functions. Interestingly, proteins that contain amino acid homorepeats that tend to evolve rapidly are enriched in ... ...

Abstract	Summary: Protein products of essential genes, indispensable for organismal survival, are highly conserved and bring about fundamental functions. Interestingly, proteins that contain amino acid homorepeats that tend to evolve rapidly are enriched in eukaryotic essentialomes. Why are proteins with hypermutable homorepeats enriched in conserved and functionally vital essential proteins? We solve this functional versus evolutionary paradox by demonstrating that human essential proteins with homorepeats bring about crosstalk across biological processes through high interactability and have distinct regulatory functions affecting expansive global regulation. Importantly, essential proteins with homorepeats rapidly diverge with the amino acid substitutions frequently affecting functional sites, likely facilitating rapid adaptability. Strikingly, essential proteins with homorepeats influence human-specific embryonic and brain development, implying that the presence of homorepeats could contribute to the emergence of human-specific processes. Thus, we propose that homorepeat-containing essential proteins affecting species-specific traits can be potential intervention targets across pathologies, including cancers and neurological disorders.
Keywords	CP: Molecular biology ; Biology (General) ; QH301-705.5
Subject code	580
Language	English
Publishing date	2023-07-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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