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Article ; Online: Acrylamide-Derived Ionome, Metabolic, and Cell Cycle Alterations Are Alleviated by Ascorbic Acid in the Fission Yeast

Marek Kovár / Alica Navrátilová / Renata Kolláthová / Anna Trakovická / Miroslava Požgajová

Molecules, Vol 27, Iss 4307, p

2022 Volume 4307

Abstract: Acrylamide (AA), is a chemical with multiple industrial applications, however, it can be found in foods that are rich in carbohydrates. Due to its genotoxic and cytotoxic effects, AA has been classified as a potential carcinogen. With the use of ... ...

Abstract	Acrylamide (AA), is a chemical with multiple industrial applications, however, it can be found in foods that are rich in carbohydrates. Due to its genotoxic and cytotoxic effects, AA has been classified as a potential carcinogen. With the use of spectrophotometry, ICP-OES, fluorescence spectroscopy, and microscopy cell growth, metabolic activity, apoptosis, ROS production, MDA formation, CAT and SOD activity, ionome balance, and chromosome segregation were determined in Schizosaccharomyces pombe . AA caused growth and metabolic activity retardation, enhanced ROS and MDA production, and modulated antioxidant enzyme activity. This led to damage to the cell homeostasis due to ionome balance disruption. Moreover, AA-induced oxidative stress caused alterations in the cell cycle regulation resulting in chromosome segregation errors, as 4.07% of cells displayed sister chromatid non-disjunction during mitosis. Ascorbic acid (AsA, Vitamin C), a strong natural antioxidant, was used to alleviate the negative impact of AA. Cell pre-treatment with AsA significantly improved AA impaired growth, and antioxidant capacity, and supported ionome balance maintenance mainly due to the promotion of calcium uptake. Chromosome missegregation was reduced to 1.79% (44% improvement) by AsA pre-incubation. Results of our multiapproach analyses suggest that AA-induced oxidative stress is the major cause of alteration to cell homeostasis and cell cycle regulation.
Keywords	acrylamide ; antioxidant capacity ; ascorbic acid ; cell cycle ; ionome ; morphology ; Organic chemistry ; QD241-441
Subject code	571
Language	English
Publishing date	2022-07-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Association of SNPs in porcine estrogen receptor gene with carcass traits

Nina Moravčíková / Anna Trakovická

Scientific Papers Animal Science and Biotechnologies, Vol 48, Iss 1, Pp 90-

2015 Volume 94

Abstract: The aim of this study was to assess the influence of estrogen receptor gene polymorphism on carcass traits in pigs’ population. In association analysis was evaluated the effect of ESR/PvuII genotypes on back fat thickness, proportion of valuable meat ... ...

Abstract	The aim of this study was to assess the influence of estrogen receptor gene polymorphism on carcass traits in pigs’ population. In association analysis was evaluated the effect of ESR/PvuII genotypes on back fat thickness, proportion of valuable meat parts, area of musculus longisimus thoracis (MLT) and proportion of thigh. In total 180 genomic DNA samples of crossbreeds (Large White x Landrace) was genotyped by using PCR-RFLP method and restriction enzyme PvuII. In population was observed prevalence of heterozygous individuals (45.56%). The lowest proportion was detected for homozygous BB genotype (13.89%). Genotype frequencies in analysed population caused the higher frequency of A allele (0.63±0.03) and relative high level of observed heterozygosity (0.46). The medium level of polymorphic information content (0.36) was found for selected locus. Association analysis was carried out by One-Way ANOVA procedure. The results showed that genotype of ESR gene influenced significantly only proportion of thigh and area of MLT (P<0.01). Average values of both traits observed for each genotype indicated positive effect of A allele present in genotype. Our results demonstrate the significant role of ESR gene for economically important traits that can be used for application in marker assisted selection programs of pigs.
Keywords	associations’ analysis ; porcine esr gene ; polymorphism ; production trait ; Agriculture ; S ; Technology ; T ; Science ; Q
Subject code	630
Language	English
Publishing date	2015-05-01T00:00:00Z
Publisher	Agroprint Timisoara
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: The Genetic Structure of Slovak Spotted Cattle Based on Genome-wide Analysis

Kristína Lehocká / Barbora Olšanská / Radovan Kasarda / Ondrej Kadlečík / Anna Trakovická / Nina Moravčíková

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, Vol 68, Iss 1, Pp 57-

2020 Volume 61

Abstract: The objective of the study was to determine the membership probability and level of admixture among Slovak Spotted cattle and historically related breeds (Ayshire, Holstein, Swiss Simmental and Slovak Pinzgau). The analysis was based on the panel of 35 ... ...

Abstract	The objective of the study was to determine the membership probability and level of admixture among Slovak Spotted cattle and historically related breeds (Ayshire, Holstein, Swiss Simmental and Slovak Pinzgau). The analysis was based on the panel of 35 934 SNPs that were used for genotyping of 423 individuals. The optimal number of clusters was estimated in two ways; by analysis of Bayesian information criterion and Bayesian clustering algorithm. The optimal number of clusters ranged from 3 to 5, depending on the applied approach. Subsequently, the population structure was tested by discriminant analysis of principal components (DAPC) and unsupervised Bayesian analysis based on the correlated allele frequencies model. The first discriminant function revealed three genetic clusters in population resulting from the production type and origin of analysed breeds. The unsupervised Bayesian analysis showed similar results, where the highest level of admixture was found between Slovak Pinzgau and Slovak Spotted cattle (0.6%). Despite that, the results of this study clearly showed that the Slovak Spotted cattle is genetically separated from other breeds that were involved in its grading-up process.
Keywords	dual-purpose breed ; DAPC analysis ; membership probability ; population structure ; Agriculture ; S ; Biology (General) ; QH301-705.5
Subject code	630
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Mendel University Press
Document type	Article ; Online
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Article ; Online: Runs of Homozygosity as Footprints of Selection in the Norik of Muran Horse Genome

Nina Moravčíková / Radovan Kasarda / Ondrej Kadlečík / Anna Trakovická / Marko Halo / Juraj Candrák

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, Vol 67, Iss 5, Pp 1165-

2019 Volume 1170

Abstract: The aim of this study was to analyse the genome-wide distribution of runs of homozygosity (ROH) segments in the genome of Norik of Muran horse and to identify the regions under strong selection pressure. Overall, 25 animals genotyped by the GGP Equine70k ...

Abstract	The aim of this study was to analyse the genome-wide distribution of runs of homozygosity (ROH) segments in the genome of Norik of Muran horse and to identify the regions under strong selection pressure. Overall, 25 animals genotyped by the GGP Equine70k chip were included in the study. After SNP pruning, 54479 SNPs (75.72%) covering 2.25 Gb of the autosomal genome were retained for scan of ROH segments distribution. The ROHs were present in the genome of all animals and covered in average 13.17% (295.29 Mb) of autosomal genome expressed by the SNP loci. The highest number of ROHs was identified on autosome 1 (404), while the lowest proportion of autosome residing in ROH showed ECA31 (38). The footprints of selection, characterized by SNPs with extreme frequency in ROHs across specific genomic regions, were defined by the top 0.01 percentile of signals. Overall, nine genomic regions located on seven autosomes (3, 6, 9, 11, 15, 23) were identified. The strongest signal of selection showed three autosomes ECA3, ECA9 and ECA11. The protein-coding genes located within these regions suggested that the identified footprints of selection are most likely consequences of intensive breeding for traits of interest during the grading-up process of the Norik of Muran horse.
Keywords	autozygosity ; horse ; genomic data ; local population ; selection sweeps ; Agriculture ; S ; Biology (General) ; QH301-705.5
Subject code	630
Language	English
Publishing date	2019-01-01T00:00:00Z
Publisher	Mendel University Press
Document type	Article ; Online
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Article ; Online: Level of Inbreeding in Norik of Muran Horse

Radovan Kasarda / Nina Moravčíková / Ondrej Kadlečík / Anna Trakovická / Marko Halo / Juraj Candrák

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, Vol 67, Iss 6, Pp 1457-

Pedigree vs. Genomic Data

2019 Volume 1463

Abstract: The objective of this study was to analyse the level of pedigree and genomic inbreeding in a herd of the Norik of Muran horses. The pedigree file included 1374 animals (603 stallions and 771 mares), while the reference population consisted of animals ... ...

Abstract	The objective of this study was to analyse the level of pedigree and genomic inbreeding in a herd of the Norik of Muran horses. The pedigree file included 1374 animals (603 stallions and 771 mares), while the reference population consisted of animals that were genotyped by using 70k SNP platform (n = 25). The trend of pedigree inbreeding was expressed as the probability that an animal has two identical alleles by descent according to classical formulas. The trend of genomic inbreeding was derived from the distribution of runs of homozygosity (ROHs) with various length in the genome based on the assumption that these regions reflect the autozygosity originated from past generations of ancestors. A maximum of 19 generations was found in pedigree file. As expected, the highest level of pedigree completeness was found in first five generations. Subsequent quality control of genomic data resulted in totally 54432 SNP markers covering 2.242 Mb of the autosomal genome. The pedigree analysis showed that in current generation can be expected the pedigree inbreeding at level 0.23% (ΔFPEDi = 0.19 ± 1.17%). Comparable results was obtained also by the genomic analysis, when the inbreeding in current generation reached level 0.11%. Thus, in term of genetic diversity both analyses reflected sufficient level of variability across analysed population of Norik of Muran horses.
Keywords	horse ; genetic diversity ; inbreeding ; local population ; Norik of Muran ; Agriculture ; S ; Biology (General) ; QH301-705.5
Subject code	630
Language	English
Publishing date	2019-01-01T00:00:00Z
Publisher	Mendel University Press
Document type	Article ; Online
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Article ; Online: The impact of diacylglycerol O-acyltransferase 1 gene polymorphism on carcass traits in cattle

Anna Trakovická / Klára Vavrišínová / Michal Gábor / Martina Miluchová / Radovan Kasarda / Nina Moravčíková

Journal of Central European Agriculture, Vol 20, Iss 1, Pp 12-

2019 Volume 18

Abstract: The aim of this study was to identify the K232A polymorphism in gene encoding diacylglycerol O-acyltransferase 1 (DGAT1) and to evaluate its effect on carcass traits in population of Slovak Pinzgau steers. The genotyping data were obtained for totally 56 ...

Abstract	The aim of this study was to identify the K232A polymorphism in gene encoding diacylglycerol O-acyltransferase 1 (DGAT1) and to evaluate its effect on carcass traits in population of Slovak Pinzgau steers. The genotyping data were obtained for totally 56 animals by using PCR-RFLP method. The A allele (0.93) was more frequent in analysed population than K allele (0.07). Even if the expected and observed heterozygosity indicated prevalence of homozygotes, the significant effect of inbreeding on population structure wasn’t found (FIS=-0.08). The decline in effective allele number (Na=1.15) as well as polymorphic information content (PIC=0.12) pointed out to significant decrease of locus effectiveness in population. The effect of DGAT1 gene polymorphism on carcass traits was tested by using the GLM procedure adopted in SAS 9.3. In association analysis the proportion of muscle, fat, bone, and drip loss within the beef three-rib section were evaluated. However, the statistical analysis showed only non-significant impact of DGAT1 gene polymorphism on selected production traits in analysed population.
Keywords	association analysis ; meat production ; Pinzgau cattle ; polymorphism ; Agriculture ; S
Subject code	630
Language	Bulgarian
Publishing date	2019-03-01T00:00:00Z
Publisher	University of Zagreb, Faculty of Agriculture
Document type	Article ; Online
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Article ; Online: SNP ANALYSES OF THE BOVINE GROWTH HORMONE AND LEPTIN GENES BY PCR – RFLP METHOD

Anna Trakovická / Nina Moravčíková

Journal of Microbiology, Biotechnology and Food Sciences, Vol 1, Iss Special issue, Pp 679-

2012 Volume 688

Abstract: The aim of this study was detection of polymorphism in the bovine growth hormone and leptin genes with using PCR-RFLP method. A polymorphic site of the growth hormone gene (AluI loci) that results in amino acid change at position 127 of the protein chain ...

Abstract	The aim of this study was detection of polymorphism in the bovine growth hormone and leptin genes with using PCR-RFLP method. A polymorphic site of the growth hormone gene (AluI loci) that results in amino acid change at position 127 of the protein chain (leucine, L to valine, V) has been linked to differences in circulating metabolites, metabolic hormones and to milk yield. The polymorphism in bovine leptin gene situated in the intron between two exons results in amino acid change at position 2059 of the protein chain (cytosine, C to thymine, T). The polymorphisms were studied in a group of 58 bull’s Slovak spotted breed. A strategy employing PCR was used to amplify a 428 bp (GH gene) and 422 bp (LEP gene) products from blood samples. Digestion of PCR products with restriction enzymes AluI and Sau3AI revealed alleles: L and V; A and B for GH gene and LEP gene, respectively. The growth hormone gene is a candidate gene for body weight gains in cattle since plays a fundamental role in growth regulation. Leptin also plays an important role in the regulation of feed intake, energy metabolism, growth and reproduction of cattle, therefore, animals with higher leptin gene expression will probably have lower daily weight gain than others with similar forage offer and nutritional condition and probably will also have longer calving interval.
Keywords	cattle ; growth hormone gene ; leptin gene ; PCR-RFLP ; polymorphism ; Biotechnology ; TP248.13-248.65 ; Chemical technology ; TP1-1185 ; Technology ; T ; DOAJ:Biotechnology ; DOAJ:Life Sciences ; DOAJ:Biology and Life Sciences ; Nutrition. Foods and food supply ; TX341-641 ; Home economics ; TX1-1110 ; DOAJ:Nutrition and Food Sciences ; DOAJ:Agriculture and Food Sciences ; Microbiology ; QR1-502 ; Science ; Q ; DOAJ:Microbiology ; DOAJ:Biology
Subject code	630
Language	English
Publishing date	2012-02-01T00:00:00Z
Publisher	Faculty of Biotechnology and Food Sciences in Nitra
Document type	Article ; Online
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Article ; Online: ANALYSIS OF CYP19 GENE POLYMORPHISM AS FACTOR AFFECTING MILK PRODUCTION OF CATTLE

Anna Trakovická / Nina Moravčíková / Martina Miluchová / Michal Gábor

Journal of Microbiology, Biotechnology and Food Sciences, Vol vol. 4, Iss special issue 2 (Biotechnology), Pp 111-

2015 Volume 113

Abstract: The aim of this study was to determine the animal CYP19/PvuII genotypes, evaluate the genetic structure of population and estimate the effect of genotypes on long-life milk production traits in Slovak Simmental cattle. Blood samples were obtained from in ...

Abstract	The aim of this study was to determine the animal CYP19/PvuII genotypes, evaluate the genetic structure of population and estimate the effect of genotypes on long-life milk production traits in Slovak Simmental cattle. Blood samples were obtained from in total 150 Slovak Simmental cows originating from four different farms localized on eastern Slovakia. The polymorphism in CYP19 gene was detected using PCR-RFLP method and PvuII restriction enzyme. In cows population was identified the presence of all three genotypes: AA (74.7%), AB (21.3%), and BB (4%). The A allele was more frequent (0.85±0.02) than B (0.15±0.02). In population was found deviations from HWE. The level of locus polymorphism in the analysed population was only low (PIC=0.212). The activity of CYP19/PvuII alleles were unbalanced (Ne=1.33) and the higher proportion of homozygous animals caused the decrease of observed heterozygosity (He=0.21). Based on F – statistic results the most of the genetic variation was distributed within all analysed individuals (FIT 0.155). Evaluation of CYP19/PvuII genotype effect on milk production traits was carried out with linear models (GLM procedure). Based on the selected effect we were able to estimate the variability of analysed traits on in average 85%. The CYP19/PvuII genotype affected significantly the variability of milk and protein yield (P<0.05). The higher production of both traits was found in individuals with BB genotype. Statistically significant influence was detected also for sire (P<0.05), breeder (P<0.0001) and total number of completed lactations (P<0.0001).
Keywords	bovine CYP19 gene ; milk production ; polymorphism ; Slovak Simmental cattle ; Biotechnology ; TP248.13-248.65 ; Chemical technology ; TP1-1185 ; Technology ; T ; Nutrition. Foods and food supply ; TX341-641 ; Home economics ; TX1-1110 ; Microbiology ; QR1-502 ; Science ; Q
Subject code	630
Language	English
Publishing date	2015-02-01T00:00:00Z
Publisher	Slovak University of Agriculture
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: ASSOCIATIONS BETWEEN SNPs IN BOVINE ESTROGEN RECEPTOR GENE AND PRODUCTION TRAITS IN HOLSTEIN CATTLE

Nina Moravčíková / Anna Trakovická / Alica Navrátilová / Rudolf Nádaský

Journal of Microbiology, Biotechnology and Food Sciences, Vol vol. 4, Iss special issue 2 (Biotechnology), Pp 83-

2015 Volume 85

Abstract: aim of this study was to determine allelic and genotypic frequency of two SNPs in ERα gene and evaluate the associations between ERα genetic variants and milk production traits in Holstein cattle. Analysis of the molecular mechanisms involved in the ... ...

Abstract	aim of this study was to determine allelic and genotypic frequency of two SNPs in ERα gene and evaluate the associations between ERα genetic variants and milk production traits in Holstein cattle. Analysis of the molecular mechanisms involved in the regulation of reproduction in connection with milk production and followed genotyping of the individuals with optimal genetic potential may facilitate the animal selection in dairy cattle farms. Genomic DNA was obtained in total from 150 hair root samples of Holstein cows. Two polymorphic sites in 5´region on ERα gene (BTA6) were analysed. Genotyping of animals was carried out by PCR-RFLP method using SnaBI and BglI restriction endonucleases. After restriction analyses was detected in population the presence of two ERα/SnaBI (GG, AG), and three ERα/BglI genotypes (GG, AG, AA). The highest proportion was found for individuals with ERα/SnaBI GG (85%) and ERα/BglI AA (83%) genotypes. The missing of ERα/SnaBI AA genotype was reflected to the higher distribution of G allele (0.92± 0.02). For the ERα/BglI polymorphism was observed the higher frequency of A allele (0.91±0.02). The differences between observed and expected genotype frequencies caused the deviations from HWE in locus ERα/SnaBI. The statistical analyses of ERα genotypes effect on milk production traits was performed with linear models (GLM procedure). Based on the selected effect we were able to estimate the variability of analyzed traits on 80%. The ERα/SnaBI and ERα/BglI genotypes affected the variability of milk, protein and fat yield only non-significant (P > 0.05).
Keywords	ERα gene ; cattle ; milk production ; polymorphisms ; Biotechnology ; TP248.13-248.65 ; Chemical technology ; TP1-1185 ; Technology ; T ; Nutrition. Foods and food supply ; TX341-641 ; Home economics ; TX1-1110 ; Microbiology ; QR1-502 ; Science ; Q
Subject code	630
Language	English
Publishing date	2015-02-01T00:00:00Z
Publisher	Slovak University of Agriculture
Document type	Article ; Online
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Article ; Online: Studies on regulation of the cell cycle in fission yeast.

Miroslava Požgajová / Peter Androvič / Anna Trakovická

Scientific Papers Animal Science and Biotechnologies, Vol 48, Iss 1, Pp 173-

2015 Volume 177

Abstract: All living organisms including plants and animals are composed of millions of cells. These cells perform different functions for the organism although they possess the same chromosomes and carry the same genetic information. Thus, to be able to ... ...

Abstract	All living organisms including plants and animals are composed of millions of cells. These cells perform different functions for the organism although they possess the same chromosomes and carry the same genetic information. Thus, to be able to understand multicellular organism we need to understand the life cycle of individual cells from which the organism comprises. The cell cycle is the life cycle of a single cell in the plant or animal body. It involves series of events in which components of the cell doubles and afterwards equally segregate into daughter cells. Such process ensures growth of the organism, and specialized reductional cell division which leads to production of gamets, assures sexual reproduction. Cell cycle is divided in the G1, S, G2 and M phase. Two gap-phases (G1 and G2) separate S phase (or synthesis) and M phase which stays either for mitosis or meiosis. Essential for normal life progression and reproduction is correct chromosome segregation during mitosis and meiosis. Defects in the division program lead to aneuploidy, which in turn leads to birth defects, miscarriages or cancer. Even thou, researchers invented much about the regulation of the cell cycle, there is still long way to understand the complexity of the regulatory machineries that ensure proper segregation of chromosomes. In this paper we would like to describe techniques and materials we use for our studies on chromosome segregation in the model organism Schizosaccharomyces pombe.
Keywords	meiosis ; mitosis ; live cell imaging ; schizosaccharomycesn pombe ; Agriculture ; S ; Technology ; T ; Science ; Q
Subject code	571
Language	English
Publishing date	2015-05-01T00:00:00Z
Publisher	Agroprint Timisoara
Document type	Article ; Online
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