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  1. Article ; Online: Lithocholic bile acid induces apoptosis in human nephroblastoma cells

    Julian Trah / Jonas Arand / Jun Oh / Laia Pagerols-Raluy / Magdalena Trochimiuk / Birgit Appl / Hannah Heidelbach / Deirdre Vincent / Moin A. Saleem / Konrad Reinshagen / Anne K. Mühlig / Michael Boettcher

    Scientific Reports, Vol 10, Iss 1, Pp 1-

    a non-selective treatment option

    2020  Volume 8

    Abstract: Abstract Lithocholic bile acid (LCA) has been reported to selectively kill cancer cells within many tumor cell lines including neuroblastoma or glioblastoma. Wilms’ tumor shares similarities with neuro- and glioblastoma. Hence, the aim of the study was ... ...

    Abstract Abstract Lithocholic bile acid (LCA) has been reported to selectively kill cancer cells within many tumor cell lines including neuroblastoma or glioblastoma. Wilms’ tumor shares similarities with neuro- and glioblastoma. Hence, the aim of the study was to evaluate the effects of LCA on nephroblastoma. To test the effects of LCA, nephroblastoma cell line WT CLS1 was used. SK NEP1 was tested as well. It was originally classified as a nephroblastoma cell line but was meanwhile reclassified as an ewing sarcoma cell line. As control cell lines HEK 293 from embryonic kidney and RC 124 from adult kidney tissue as well as podocytes were used. The effects were evaluated using proliferation assay, caspase activity assay, FACS and Western blot. LCA showed a dose and time-dependent selective effect inducing apoptosis in nephroblastoma cells. However, these effects were not limited to the nephroblastoma cell line but also affected control kidney cell lines and the sarcoma cells; only podocytes are significantly less affected by LCA (at dosages < 200 µm). There were no significant differences regarding the TGR5 receptor expression. The study showed that LCA has a strong, yet unselective effect on all used in vitro cell-lines, sparing the highly differentiated podocytes in lower concentrations. Further studies are needed to verify our results before dismissing LCA as an anti-cancer drug.
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2020-11-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Multicytokine-producing CD4+ T cells characterize the livers of patients with NASH

    Anna Woestemeier / Pasquale Scognamiglio / Yu Zhao / Jonas Wagner / Franziska Muscate / Christian Casar / Francesco Siracusa / Filippo Cortesi / Theodora Agalioti / Simone Müller / Adrian Sagebiel / Leonie Konczalla / Ramez Wahib / Karl-Frederick Karstens / Anastasios D. Giannou / Anna Duprée / Stefan Wolter / Milagros N. Wong / Anne K. Mühlig /
    Agata A. Bielecka / Vikas Bansal / Tianran Zhang / Oliver Mann / Victor G. Puelles / Tobias B. Huber / Ansgar W. Lohse / Jakob R. Izbicki / Noah W. Palm / Stefan Bonn / Samuel Huber / Nicola Gagliani

    JCI Insight, Vol 8, Iss

    2023  Volume 1

    Abstract: A role of CD4+ T cells during the progression from nonalcoholic fatty liver disease (NAFLD) to nonalcoholic steatohepatitis (NASH) has been suggested, but which polarization state of these cells characterizes this progression and the development of ... ...

    Abstract A role of CD4+ T cells during the progression from nonalcoholic fatty liver disease (NAFLD) to nonalcoholic steatohepatitis (NASH) has been suggested, but which polarization state of these cells characterizes this progression and the development of fibrosis remain unclear. In addition, a gut-liver axis has been suggested to play a role in NASH, but the role of CD4+ T cells in this axis has just begun to be investigated. Combining single-cell RNA sequencing and multiple-parameter flow cytometry, we provide the first cell atlas to our knowledge focused on liver-infiltrating CD4+ T cells in patients with NAFLD and NASH, showing that NASH is characterized by a population of multicytokine-producing CD4+ T cells. Among these cells, only those with a Th17 polarization state were enriched in patients with advanced fibrosis. In parallel, we observed that Bacteroides appeared to be enriched in the intestine of NASH patients and to correlate with the frequency of multicytokine-producing CD4+ T cells. In short, we deliver a CD4+ T cell atlas of NAFLD and NASH, providing the rationale to target CD4+ T cells with a Th17 polarization state to block fibrosis development. Finally, our data offer an early indication to test whether multicytokine-producing CD4+ T cells are part of the gut-liver axis characterizing NASH.
    Keywords Hepatology ; Immunology ; Medicine ; R
    Subject code 570
    Language English
    Publishing date 2023-01-01T00:00:00Z
    Publisher American Society for Clinical investigation
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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