Article: Extracellular matrix gene expression profiling using microfluidics for colorectal carcinoma stratification.
Biomicrofluidics
2016 Volume 10, Issue 5, Page(s) 54124
Abstract: In cancer, biomarkers have many potential applications including generation of a differential diagnosis, prediction of response to treatment, and monitoring disease progression. Many molecular biomarkers have been put forward for different diseases but ... ...
| Abstract | In cancer, biomarkers have many potential applications including generation of a differential diagnosis, prediction of response to treatment, and monitoring disease progression. Many molecular biomarkers have been put forward for different diseases but most of them do not possess the required specificity and sensitivity. A biomarker with a high sensitivity has a low specificity and vice versa. The inaccuracy of the biomarkers currently in use has led to a compelling need to identify more accurate markers with diagnostic and prognostic significance. The aim of the present study was to use a novel, droplet-based, microfluidic platform to evaluate the prognostic value of a panel of thirty-four genes that regulate the composition of extracellular matrices in colorectal carcinoma. Our method is a novel approach as it uses using continuous-flowing Polymerase Chain Reaction for the sensitive detection and accurate quantitation of gene expression. We identified a panel of relevant extracellular matrix genes whose expression levels were measured by real-time quantitative polymerase chain reaction using Taqman |
|---|---|
| Language | English |
| Publishing date | 2016-10-31 |
| Publishing country | United States |
| Document type | Journal Article |
| ISSN | 1932-1058 |
| ISSN | 1932-1058 |
| DOI | 10.1063/1.4966245 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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