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  1. Article ; Online: Identification of potential vaccine targets for elicitation of host immune cells against SARS-CoV-2 by reverse vaccinology approach.

    Yasmin, Sabina / Ansari, Mohammad Yousuf / Pandey, Krishna / Dikhit, Manas Ranjan

    International journal of biological macromolecules

    2024  Volume 265, Issue Pt 2, Page(s) 130754

    Abstract: The COVID-19 pandemic has emerged as a critical global health crisis, demanding urgent and effective strategies for containment. While some knowledge exists about epitope sequences recognized by human immune cells and their activation of CD8+ T cells ... ...

    Abstract The COVID-19 pandemic has emerged as a critical global health crisis, demanding urgent and effective strategies for containment. While some knowledge exists about epitope sequences recognized by human immune cells and their activation of CD8+ T cells within the HLA context, comprehensive information remains limited. This study employs reverse vaccinology to explore antigenic HLA-restricted T-cell epitopes capable of eliciting durable immunity. Screening reveals 187 consensus epitopes, with 23 offering broad population coverage worldwide, spanning over 5000 HLA alleles. Sequence alignment analysis highlights the genetic distinctiveness of these peptides from Homo sapiens and their intermediate to high TAP binding efficiency. Notably, these epitopes share 100 % sequence identity across strains from nine countries, indicating potential for a uniform protective immune response among diverse ethnic populations. Docking simulations further confirm their binding capacity with the HLA allele, validating them as promising targets for SARS-CoV-2 immune recognition. The anticipated epitopes are connected with suitable linkers and adjuvant, and then assessed for its translational efficacy within a bacterial expression vector through computational cloning. Through docking, it is observed that the chimeric vaccine construct forms lasting hydrogen bonds with Toll-like receptor (TLR4), while immune simulation illustrates an increased cytotoxic response aimed at CD8+ T cells. This comprehensive computational analysis suggests the chimeric vaccine construct's potential to provoke a robust immune response against SARS-CoV-2. By delineating these antigenic fragments, our study offers valuable insights into effective vaccine and immunotherapy development against COVID-19, contributing significantly to global efforts in combating this infectious threat.
    MeSH term(s) Humans ; SARS-CoV-2 ; COVID-19/prevention & control ; Vaccinology ; Pandemics/prevention & control ; Molecular Docking Simulation ; Viral Vaccines ; Epitopes, T-Lymphocyte/chemistry ; Epitopes, B-Lymphocyte ; Computational Biology ; Vaccines, Subunit
    Chemical Substances Viral Vaccines ; Epitopes, T-Lymphocyte ; Epitopes, B-Lymphocyte ; Vaccines, Subunit
    Language English
    Publishing date 2024-03-19
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2024.130754
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 2374: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  2. Article: Are Proton Pump Inhibitors Contributing in Emerging New Hypertensive Population?

    Tayal, Rohit / Yasmin, Sabina / Chauhan, Samrat / Singh, Thakur Gurjeet / Saini, Monika / Shorog, Eman / Althubyani, Maryam M / Alsaadi, Baiaan H / Aljohani, Fatimah / Alenazi, Maram A / Abutaily, Sarah A / Ansari, Mohammad Yousuf

    Pharmaceuticals (Basel, Switzerland)

    2023  Volume 16, Issue 10

    Abstract: Balancing the therapeutic advantages of a medicine with its possible risks and side effects is an important part of medical practice and drug regulation. When a drug is designed to treat a particular disease or medical condition ends up causing ... ...

    Abstract Balancing the therapeutic advantages of a medicine with its possible risks and side effects is an important part of medical practice and drug regulation. When a drug is designed to treat a particular disease or medical condition ends up causing additional risks or side effects that lead to the development of other serious health problems, it can have detrimental consequences for patients. This article explores the correlation between persistent proton pump inhibitor (PPI) use and hypertension, a common cardiovascular ailment. While PPIs are beneficial in treating various gastrointestinal problems, their availability without a prescription has resulted in self-medication and long-term use without medical monitoring. Recent findings have revealed a link between long-term PPI usage and increased cardiovascular risks, particularly hypertension. This study investigates the intricate mechanisms underlying PPI's effects, focusing on potential pathways contributing to hypertension, such as endothelial dysfunction, disruption of nitric oxide bioavailability, vitamin B deficiency, hypocalcemia, and hypomagnesemia. The discussion explains how long-term PPI use can disrupt normal endothelial function, vascular control, and mineral balance, eventually leading to hypertension. The article emphasizes the significance of using PPIs with caution and ongoing research to better understand the implications of these medications on cardiovascular health.
    Language English
    Publishing date 2023-09-30
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2193542-7
    ISSN 1424-8247
    ISSN 1424-8247
    DOI 10.3390/ph16101387
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Novel Drug Delivery Systems as an Emerging Platform for Stomach Cancer Therapy.

    Hani, Umme / Osmani, Riyaz Ali M / Yasmin, Sabina / Gowda, B H Jaswanth / Ather, Hissana / Ansari, Mohammad Yousuf / Siddiqua, Ayesha / Ghazwani, Mohammed / Fatease, Adel Al / Alamri, Ali H / Rahamathulla, Mohamed / Begum, M Yasmin / Wahab, Shadma

    Pharmaceutics

    2022  Volume 14, Issue 8

    Abstract: Cancer has long been regarded as one of the world's most fatal diseases, claiming the lives of countless individuals each year. Stomach cancer is a prevalent cancer that has recently reached a high number of fatalities. It continues to be one of the most ...

    Abstract Cancer has long been regarded as one of the world's most fatal diseases, claiming the lives of countless individuals each year. Stomach cancer is a prevalent cancer that has recently reached a high number of fatalities. It continues to be one of the most fatal cancer forms, requiring immediate attention due to its low overall survival rate. Early detection and appropriate therapy are, perhaps, of the most difficult challenges in the fight against stomach cancer. We focused on positive tactics for stomach cancer therapy in this paper, and we went over the most current advancements and progressions of nanotechnology-based systems in modern drug delivery and therapies in great detail. Recent therapeutic tactics used in nanotechnology-based delivery of drugs aim to improve cellular absorption, pharmacokinetics, and anticancer drug efficacy, allowing for more precise targeting of specific agents for effective stomach cancer treatment. The current review also provides information on ongoing research aimed at improving the curative effectiveness of existing anti-stomach cancer medicines. All these crucial matters discussed under one overarching title will be extremely useful to readers who are working on developing multi-functional nano-constructs for improved diagnosis and treatment of stomach cancer.
    Language English
    Publishing date 2022-07-29
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2527217-2
    ISSN 1999-4923
    ISSN 1999-4923
    DOI 10.3390/pharmaceutics14081576
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: High resolution cryo-EM structures of two potently SARS-CoV-2 neutralizing monoclonal antibodies of same donor origin that vary in neutralizing Omicron variants

    Rencilin, Clayton Fernando / Ansari, Mohammad Yousuf / Chatterjee, Arnab / Deshpande, Suprit / Mukherjee, Sohini / Singh, Randhir / Jayatheertha, Sowrabha / Reddy, Poorvi M. / Das, Payel / Hingankar, Nitin / Rathore, Deepak / Varadarajan, Raghavan / Bhattacharya, Jayanta / Dutta, Somnath

    bioRxiv

    Abstract: While vaccines have by large been found to effective against the evolving SARS-CoV-2 variants, the profound and rapid effectivity of monoclonal antibodies (mAbs) in significantly reducing hospitalization to severe disease outcomes have also been ... ...

    Abstract While vaccines have by large been found to effective against the evolving SARS-CoV-2 variants, the profound and rapid effectivity of monoclonal antibodies (mAbs) in significantly reducing hospitalization to severe disease outcomes have also been demonstrated. In the present study, by high resolution cryo-electron microscopy (cryo-EM), we examined the structural insights of two trimeric spike (S) protein bound mAbs isolated from an Indian convalescent individual infected with ancestral SARS-CoV-2 which we recently reported to potently neutralize SARS-CoV-2 from its ancestral form through highly virulent Delta form however different in their ability to neutralize Omicron variants. Our findings showed binding and conformational heterogeneities of both the mAbs (THSC20.HVTR04 and THSC20.HVTR26) bound to S trimer in its apo and hACE-2 bound forms. Additionally, cryo-EM resolved structure assisted modeling highlighted key residues associated with the ability of these two mAbs to neutralize Omicron variants. Our findings highlighted key interacting features modulating antigen-antibody interacting that can further aid in structure guided antibody engineering to enhance their breadth and potency.
    Keywords covid19
    Language English
    Publishing date 2022-12-05
    Publisher Cold Spring Harbor Laboratory
    Document type Article ; Online
    DOI 10.1101/2022.12.03.518949
    Database COVID19

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  5. Article ; Online: Tuberculosis: current treatment, diagnostics, and newer antitubercular agents in clinical trials.

    Ahsan, Mohamed Jawed / Ansari, Mohammad Yousuf / Yasmin, Sabina / Jadav, Surender Singh / Kumar, Pradeep / Garg, Shiv Kumar / Aseri, Ajay / Khalilullah, Habibullah

    Infectious disorders drug targets

    2014  Volume 15, Issue 1, Page(s) 32–41

    Abstract: Tuberculosis (TB), a dreadful disease is one of the most important health problems worldwide, and is responsible for approximately 1.3 million death tolls in 2012. DOTS is the currently used drug therapy in TB and the long term drug regimens and patients' ...

    Abstract Tuberculosis (TB), a dreadful disease is one of the most important health problems worldwide, and is responsible for approximately 1.3 million death tolls in 2012. DOTS is the currently used drug therapy in TB and the long term drug regimens and patients' poor compliance lead to emergence of multidrug resistant (MDR) and extensively drug resistant (XDR) TB, which invigorates the research efforts to address the urgent need for the quick diagnosis and for newer antitubercular agents and vaccines to completely eradicate TB. Today we have at least 20 new diagnostic test platforms, 14 TB vaccine candidates in clinical trials and over 35 candidates in preclinical development, and among the antitubercular agents under clinical investigation, 4 anti-TB agents are in Phase III (efficacy) trials and 7 anti-TB agents are in Phase II, early bactericidal activity and sputum culture conversion trials (rifapentine is in a Phase II and a Phase III trial), 5 anti-TB agents in preclinical development and 3 anti-TB agents in Good Laboratory Practice toxicity evaluation. Recently US FDA has approved TMC207 as a part of combination therapy to treat adults with MDR pulmonary TB in the absence of other alternatives. We provide here the concise review on the chemical entities currently in the clinical trials, the new vaccines in the developmental pipeline, and the new diagnostic test.
    MeSH term(s) Adult ; Antitubercular Agents/therapeutic use ; Clinical Trials as Topic ; Diarylquinolines/therapeutic use ; Diarylquinolines/toxicity ; Directly Observed Therapy ; Drug Discovery ; Drug Resistance, Bacterial ; Drug Therapy, Combination ; Humans ; Mycobacterium tuberculosis/drug effects ; Rifampin/analogs & derivatives ; Rifampin/therapeutic use ; Rifampin/toxicity ; Tuberculosis/diagnosis ; Tuberculosis/drug therapy ; Tuberculosis/prevention & control ; Tuberculosis Vaccines/therapeutic use
    Chemical Substances Antitubercular Agents ; Diarylquinolines ; Tuberculosis Vaccines ; bedaquiline (78846I289Y) ; Rifampin (VJT6J7R4TR) ; rifapentine (XJM390A33U)
    Language English
    Publishing date 2014-09-04
    Publishing country United Arab Emirates
    Document type Journal Article ; Review
    ZDB-ID 2234298-9
    ISSN 2212-3989 ; 1871-5265
    ISSN (online) 2212-3989
    ISSN 1871-5265
    DOI 10.2174/1871526514666140923153329
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5598: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: Mining the Proteome of Leishmania donovani for the Development of Novel MHC Class I Restricted Epitope for the Control of Visceral Leishmaniasis.

    Dikhit, Manas R / Kumar, Akhilesh / Amit, Ajay / Dehury, Budheswar / Nathsharma, Yangya Prasad / Ansari, Mohammad Yousuf / Ali, Vahab / Topno, Roshan Kamal / Das, Vnr / Pandey, Krishna / Sahoo, Ganesh Chandra / Bimal, Sanjiva / Das, Pradeep

    Journal of cellular biochemistry

    2017  

    Abstract: Although, the precise host defence mechanism(s) is not completely understood, T cell-mediated immune responses is believed to play a pivotal role in controlling parasite infection. Here we target the stage dependent over expressed gene. Here, the ... ...

    Abstract Although, the precise host defence mechanism(s) is not completely understood, T cell-mediated immune responses is believed to play a pivotal role in controlling parasite infection. Here we target the stage dependent over expressed gene. Here, the consensus based computational approach was adopted for the screening of potential major histocompatibility complex class I restricted epitopes. Based on the computational analysis and previously published report, a set 19 antigenic proteins derived from Leishmania donovani were screened for further characterization as vaccine candidates. A total of 49 epitopes were predicted, which revealed a comprehensive binding affinity to the 40 different MHC class I supertypes. Based on the population coverage and HLA cross presentation, nine highly promiscuous epitopes such as LTYDDVWTV (P1), FLFPQRTAL(P2), FLFSNGAVV (P3), YIYNFGIRV (P4), YMTAAFAAL (P5), KLLRPFAPL (P6), FMLGWIVTI (P7), SLFERNKRV (P8), and SVWNRIFTL (P9) which have either a high or an intermediate TAP binding affinity were selected for further analysis. Theoretical population coverage analysis of polytope vaccine (P1-P9) revealed more than 92% population. Stimulation with the cocktail of peptide revealed a proliferative CD8
    Language English
    Publishing date 2017-06-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 392402-6
    ISSN 1097-4644 ; 0730-2312
    ISSN (online) 1097-4644
    ISSN 0730-2312
    DOI 10.1002/jcb.26190
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1114: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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