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  1. Article ; Online: Anterior Sphincter-sparing Suturing of the Vesicourethral Anastomosis During Robotic-assisted Laparoscopic Radical Prostatectomy.

    Antonelli, Luca / Afferi, Luca / Mattei, Agostino / Fankhauser, Christian Daniel

    European urology open science

    2023  Volume 52, Page(s) 109–114

    Abstract: Background: Continence is an important functional outcome after robotic-assisted laparoscopic radical prostatectomy (RARP), and modifications of the surgical technique may improve outcomes.: Objective: To illustrate a novel RARP technique and to ... ...

    Abstract Background: Continence is an important functional outcome after robotic-assisted laparoscopic radical prostatectomy (RARP), and modifications of the surgical technique may improve outcomes.
    Objective: To illustrate a novel RARP technique and to describe the observed continence outcomes.
    Design setting and participants: A retrospective study of men treated with RARP between 2017 and 2021 was conducted.
    Surgical procedure: During RARP, periprostatic structures are preserved, the intraprostatic urethra is partially spared, and the anterior anastomosis stitches involve the plexus structures but not the anterior urethra.
    Measurements: A descriptive analysis of the pathological, functional, and short-term oncological outcomes was performed.
    Results and limitations: Of 640 men, 448 (70%) with at least 1 yr of follow-up and a median age of 66 yr were included. The median operative time was 270 min and the prostatic volume 52 ml. The transurethral catheter was removed after a median of 3 d, and leakage of urine in the first 24 h after catheter removal was observed in 66/448 patients (15%). Positive surgical margins were reported in 104/448 (23%). Prostate-specific antigen persistence after prostatectomy was observed in 26/448 (6%). During a median follow-up of 2 yr (interquartile range 1-3 yr), the biochemical recurrence after prostatectomy was observed in 19/448 patients (4%). One year after prostatectomy, 406/448 patients (91%) were continent and required no pad at all, while 42/448 (9%) required at least one pad per day.
    Conclusions: Not stitching the anterior urethra is a novel technical modification and may improve continence outcomes.
    Patient summary: We describe a novel way to stitch the bladder neck to the urethra after removal of the prostate using a surgical robotic system. Our technique appeared safe, with promising urinary continence results.
    Language English
    Publishing date 2023-05-05
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 3040546-4
    ISSN 2666-1683 ; 2058-4881
    ISSN (online) 2666-1683
    ISSN 2058-4881
    DOI 10.1016/j.euros.2023.04.007
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: PhaseX: an X-ray phase-contrast imaging simulation code for matter under extreme conditions.

    Barbato, Francesco / Atzeni, Stefano / Batani, Dimitri / Antonelli, Luca

    Optics express

    2022  Volume 30, Issue 3, Page(s) 3388–3403

    Abstract: We present PhaseX, a simulation code for X-ray phase-contrast imaging (XPCI), specially dedicated to the study of matter under extreme conditions (of pressure and density). Indeed, XPCI can greatly benefit the diagnosis of such states of matter. This is ... ...

    Abstract We present PhaseX, a simulation code for X-ray phase-contrast imaging (XPCI), specially dedicated to the study of matter under extreme conditions (of pressure and density). Indeed, XPCI can greatly benefit the diagnosis of such states of matter. This is due to the noticeable contrast enhancement obtained thanks to the exploitation of both attenuation and phase-shift of the electromagnetic waves crossing the sample to be diagnosed. PhaseX generates synthetic images with and without phase contrast. Thanks to its modular design PhaseX can adapt to any imaging set-up and accept as inputs objects generated by hydrodynamic or particle-in-cell codes. We illustrate Phase-X capabilities by showing a few examples concerning laser-driven implosions and laser-driven shock waves.
    Language English
    Publishing date 2022-02-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1491859-6
    ISSN 1094-4087 ; 1094-4087
    ISSN (online) 1094-4087
    ISSN 1094-4087
    DOI 10.1364/OE.448479
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: An enantioselective high-performance liquid chromatography-mass spectrometry method to study the fate of quizalofop-P-ethyl in soil and selected agricultural products

    Antonelli, Lorenzo / Lucci, Elena / Fanali, Salvatore / Fanali, Chiara / Gentili, Alessandra / Chankvetadze, Bezhan

    Journal of Chromatography A. 2023 Aug. 09, p.464289-

    2023  , Page(s) 464289–

    Abstract: In this study, the attention was focused on quizalofop-ethyl, a chiral herbicide whose formulation has recently been marketed as quizalofop-P-ethyl, i.e. the (+)-enantiomer exhibiting herbicidal activity. To verify the real enantiomeric purity of this ... ...

    Abstract In this study, the attention was focused on quizalofop-ethyl, a chiral herbicide whose formulation has recently been marketed as quizalofop-P-ethyl, i.e. the (+)-enantiomer exhibiting herbicidal activity. To verify the real enantiomeric purity of this product as well as to study its environmental fate, the enantioselective separation of the P- and M- enantiomers of quizalofop-ethyl was achieved on Lux Cellulose-2 column (3-chloro,4-methylphenilcarbamate cellulose) under isocratic organic polar mode. Once established that the commercial formulation contains ⁓ 0.6% (enantiomeric fraction) of M as an impurity, the HPLC-MS/MS method was applied to the analysis of real samples (soil, carrots and turnips) treated with it, upon preliminary method validation on each of the selected matrices. A simple solid-liquid extraction allowed recoveries greater than 70%; limits of detections of P and M enentiomers were below 5 ng g⁻¹. The analyses showed a modification of the enantiomeric fraction of quizalofop-M-ethyl between the commercial formulation (EFM = 0.63 ± 0.03%) and the analysed samples (EFM = 7.6 ± 0.1% for carrots; EFM = 0% for the other matrices). This outcome has highlighted the occurrence of an enantioselective biotic dissipation, responsible for a greater persistency of the distomer in carrots. On the other hand, since screening analyses revealed the occurrence of residues of the metabolite quizalofop-acid with the same EFs as the ester precursor, it was concluded that the hydrolytic conversion was an abiotic process.
    Keywords cellulose ; enantiomers ; enantioselectivity ; environmental fate ; herbicidal properties ; liquid chromatography ; mass spectrometry ; metabolites ; quizalofop ; soil ; Quizalofop-ethyl ; pesticides ; high performance liquid chromatography ; polysaccharide-based chiral selectors ; enantioselective chromatography
    Language English
    Dates of publication 2023-0809
    Publishing place Elsevier B.V.
    Document type Article ; Online
    Note Pre-press version
    ZDB-ID 218139-3
    ISSN 0021-9673 ; 0378-4355 ; 0376-737X
    ISSN 0021-9673 ; 0378-4355 ; 0376-737X
    DOI 10.1016/j.chroma.2023.464289
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Shock-Augmented Ignition Approach to Laser Inertial Fusion.

    Scott, R H H / Barlow, D / Trickey, W / Ruocco, A / Glize, K / Antonelli, L / Khan, M / Woolsey, N C

    Physical review letters

    2022  Volume 129, Issue 19, Page(s) 195001

    Abstract: Shock ignition enables high gain at low implosion velocity, reducing ablative Rayleigh-Taylor instability growth, which can degrade conventional direct drive. With this method, driving a strong shock requires high laser power and intensity, resulting in ... ...

    Abstract Shock ignition enables high gain at low implosion velocity, reducing ablative Rayleigh-Taylor instability growth, which can degrade conventional direct drive. With this method, driving a strong shock requires high laser power and intensity, resulting in inefficiencies in the drive and the generation of hot electrons that can preheat the fuel. A new "shock-augmented ignition" pulse shape is described that, by preconditioning the ablation plasma before launching a strong shock, enables the shock ignition of thermonuclear fuel, but importantly, with substantially reduced laser power and intensity requirements. The reduced intensity requirement with respect to shock ignition limits laser-plasma instabilities, such as stimulated Raman and Brillouin scatter, reducing the risk of hot-electron preheat and restoring the laser coupling advantages of conventional direct drive. Simulations indicate that, due to the reduced power requirements, high gain (∼100) ignition of large-scale direct drive implosions (outer radius ∼1750  μm, deuterium-tritium ice thickness ∼165  μm) may be possible within the power and energy limits of existing facilities such as the National Ignition Facility. Moreover, this concept extends to indirect drive implosions, which exhibit substantial yield increases at reduced implosion velocity. Shock-augmented ignition expands the viable design space of laser inertial fusion.
    Language English
    Publishing date 2022-11-17
    Publishing country United States
    Document type Journal Article
    ZDB-ID 208853-8
    ISSN 1079-7114 ; 0031-9007
    ISSN (online) 1079-7114
    ISSN 0031-9007
    DOI 10.1103/PhysRevLett.129.195001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: An enantioselective high-performance liquid chromatography-mass spectrometry method to study the fate of quizalofop-P-ethyl in soil and selected agricultural products.

    Antonelli, Lorenzo / Lucci, Elena / Fanali, Salvatore / Fanali, Chiara / Gentili, Alessandra / Chankvetadze, Bezhan

    Journal of chromatography. A

    2023  Volume 1707, Page(s) 464289

    Abstract: In this study, the attention was focused on quizalofop-ethyl, a chiral herbicide whose formulation has recently been marketed as quizalofop-P-ethyl, i.e. the (+)-enantiomer exhibiting herbicidal activity. To verify the real enantiomeric purity of this ... ...

    Abstract In this study, the attention was focused on quizalofop-ethyl, a chiral herbicide whose formulation has recently been marketed as quizalofop-P-ethyl, i.e. the (+)-enantiomer exhibiting herbicidal activity. To verify the real enantiomeric purity of this product as well as to study its environmental fate, the enantioselective separation of the P- and M- enantiomers of quizalofop-ethyl was achieved on Lux Cellulose-2 column (3‑chloro,4-methylphenilcarbamate cellulose) under isocratic conditions in polar organic mode. Once established that the commercial formulation contains ˜ 0.6% (enantiomeric fraction) of M as an impurity, an HPLC-MS/MS method was developed, validated and applied to the analysis of soil, carrots and turnips treated with the herbicide. A simple solid-liquid extraction allowed recoveries greater than 70%; limits of detections of P and M enantiomers were below 5 ng g
    MeSH term(s) Chromatography, High Pressure Liquid/methods ; Soil/chemistry ; Tandem Mass Spectrometry/methods ; Stereoisomerism ; Herbicides/analysis
    Chemical Substances quizalofop (137F325077) ; quizalofop-ethyl (7280704RL4) ; Soil ; Herbicides
    Language English
    Publishing date 2023-08-09
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1171488-8
    ISSN 1873-3778 ; 0021-9673
    ISSN (online) 1873-3778
    ISSN 0021-9673
    DOI 10.1016/j.chroma.2023.464289
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Segmentation, 3D Reconstruction, and Analysis of PcG Proteins in Fluorescence Microscopy Images in Different Cell Culture Conditions.

    Gregoretti, Francesco / Lucini, Federica / Cesarini, Elisa / Oliva, Gennaro / Lanzuolo, Chiara / Antonelli, Laura

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2655, Page(s) 147–169

    Abstract: Polycomb-group (PcG) of proteins are evolutionarily conserved transcription factors necessary for the regulation of gene expression during the development and the safeguard of cell identity in adulthood. In the nucleus, they form aggregates whose ... ...

    Abstract Polycomb-group (PcG) of proteins are evolutionarily conserved transcription factors necessary for the regulation of gene expression during the development and the safeguard of cell identity in adulthood. In the nucleus, they form aggregates whose positioning and dimension are fundamental for their function. We present an algorithm, and its MATLAB implementation, based on mathematical methods to detect and analyze PcG proteins in fluorescence cell image z-stacks. Our algorithm provides a method to measure the number, the size, and the relative positioning of the PcG bodies in the nucleus for a better understanding of their spatial distribution, and thus of their role for a correct genome conformation and function.
    MeSH term(s) Polycomb-Group Proteins ; Imaging, Three-Dimensional ; Cell Nucleus/metabolism ; Cell Culture Techniques ; Microscopy, Fluorescence/methods
    Chemical Substances Polycomb-Group Proteins
    Language English
    Publishing date 2023-05-22
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3143-0_12
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Nanocomposite microbeads made of recycled polylactic acid for the magnetic solid phase extraction of xenobiotics from human urine.

    Antonelli, Lorenzo / Frondaroli, Maria Chiara / De Cesaris, Massimo Giuseppe / Felli, Nina / Dal Bosco, Chiara / Lucci, Elena / Gentili, Alessandra

    Mikrochimica acta

    2024  Volume 191, Issue 5, Page(s) 251

    Abstract: Nanocomposite microbeads (average diameter = 10-100 µm) were prepared by a microemulsion-solidification method and applied to the magnetic solid-phase extraction (m-SPE) of fourteen analytes, among pesticides, drugs, and hormones, from human urine ... ...

    Abstract Nanocomposite microbeads (average diameter = 10-100 µm) were prepared by a microemulsion-solidification method and applied to the magnetic solid-phase extraction (m-SPE) of fourteen analytes, among pesticides, drugs, and hormones, from human urine samples. The microbeads, perfectly spherical in shape to maximize the surface contact with the analytes, were composed of magnetic nanoparticles dispersed in a polylactic acid (PLA) solid bulk, decorated with multi-walled carbon nanotubes (mPLA@MWCNTs). In particular, PLA was recovered from filters of smoked electronic cigarettes after an adequate cleaning protocol. A complete morphological characterization of the microbeads was performed via Fourier-transform infrared (FTIR) spectroscopy, UV-Vis spectroscopy, thermogravimetric and differential scanning calorimetry analysis (TGA and DSC), scanning electron microscopy (SEM) and X-ray diffraction analysis (XRD). The recovery study of the m-SPE procedure showed yields ≥ 64%, with the exception of 4-chloro-2-methylphenol (57%) at the lowest spike level (3 µg L
    MeSH term(s) Humans ; Nanotubes, Carbon/chemistry ; Xenobiotics ; Electronic Nicotine Delivery Systems ; Microspheres ; Polyesters ; Solid Phase Extraction/methods ; Nanocomposites/chemistry ; Magnetic Phenomena
    Chemical Substances poly(lactide) (459TN2L5F5) ; Nanotubes, Carbon ; Xenobiotics ; Polyesters
    Language English
    Publishing date 2024-04-09
    Publishing country Austria
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 89-9
    ISSN 1436-5073 ; 0026-3672
    ISSN (online) 1436-5073
    ISSN 0026-3672
    DOI 10.1007/s00604-024-06335-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Recovery of cellulose acetate bioplastic from cigarette butts: realization of a sustainable sorbent for water remediation.

    De Cesaris, Massimo Giuseppe / Felli, Nina / Antonelli, Lorenzo / Francolini, Iolanda / D'Orazio, Giovanni / Dal Bosco, Chiara / Gentili, Alessandra

    The Science of the total environment

    2024  Volume 929, Page(s) 172677

    Abstract: Cigarette butts, one of the most common forms of litter in the world, represent a source of chemical and plastic pollution releasing thousands of toxic compounds and microfibers of cellulose acetate (CA). Besides the correct waste management, the ... ...

    Abstract Cigarette butts, one of the most common forms of litter in the world, represent a source of chemical and plastic pollution releasing thousands of toxic compounds and microfibers of cellulose acetate (CA). Besides the correct waste management, the recovery of CA from cigarette filters is a way to cushion their negative effects on the environment. Thus far, recycling strategies have been limited to industrial applications, while not many solutions have designed for water remediation. This work describes a strategy to valorize this harmful waste and to reverse its environmental impact, proposing a simple and effective procedure of reclamation of CA and its reuse to prepare a composite sorbent for the treatment of polluted water. The first step entails the washing of filters with hot water (T = 90 °C) and hot ethanol (T = 58-68 °C) to remove the impurities produced during cigarette burning, as verified by means of UV and attenuated total reflection-Fourier-transform infrared (ATR-FTIR) spectroscopy, thermal gravimetric analysis (TGA), and differential scanning calorimetry (DSC). The second step involves the use of the regenerated CA to prepare porous cylinder-shaped cryogels (15 mm × 10 mm) whose sorption properties are enhanced by the combination with AC (15 % w/w). The synthesis takes advantage of the sol-gel transition of the polymer dispersion (5 % w/V) in a solution acetone/water 5 mM in NH
    Language English
    Publishing date 2024-04-24
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2024.172677
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Book ; Online: Cartoon-texture evolution for two-region image segmentation

    Antonelli, Laura / De Simone, Valentina / Viola, Marco

    2022  

    Abstract: Two-region image segmentation is the process of dividing an image into two regions of interest, i.e., the foreground and the background. To this aim, Chan et al. [Chan, Esedo\=glu, Nikolova, SIAM Journal on Applied Mathematics 66(5), 1632-1648, 2006] ... ...

    Abstract Two-region image segmentation is the process of dividing an image into two regions of interest, i.e., the foreground and the background. To this aim, Chan et al. [Chan, Esedo\=glu, Nikolova, SIAM Journal on Applied Mathematics 66(5), 1632-1648, 2006] designed a model well suited for smooth images. One drawback of this model is that it may produce a bad segmentation when the image contains oscillatory components. Based on a cartoon-texture decomposition of the image to be segmented, we propose a new model that is able to produce an accurate segmentation of images also containing noise or oscillatory information like texture. The novel model leads to a non-smooth constrained optimization problem which we solve by means of the ADMM method. The convergence of the numerical scheme is also proved. Several experiments on smooth, noisy, and textural images show the effectiveness of the proposed model.

    Comment: 26 pages, 2 tables, 6 figures
    Keywords Mathematics - Numerical Analysis ; Computer Science - Computer Vision and Pattern Recognition ; 90C25 ; 65K05 ; 94A08
    Subject code 004
    Publishing date 2022-03-07
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Characterization and Authentication of "Ricotta" Whey Cheeses through GC-FID Analysis of Fatty Acid Profile and Chemometrics.

    Biancolillo, Alessandra / Reale, Samantha / Foschi, Martina / Bertini, Emanuele / Antonelli, Lavinia / D'Archivio, Angelo Antonio

    Molecules (Basel, Switzerland)

    2022  Volume 27, Issue 21

    Abstract: The fatty acid (FA) profiles of 240 samples of ricotta whey cheese made from sheep, goat, cow, or water buffalo milk were analyzed by gas-chromatography (GC). Then, sequential preprocessing through orthogonalization (SPORT) was used in order to classify ... ...

    Abstract The fatty acid (FA) profiles of 240 samples of ricotta whey cheese made from sheep, goat, cow, or water buffalo milk were analyzed by gas-chromatography (GC). Then, sequential preprocessing through orthogonalization (SPORT) was used in order to classify samples according to the nature of the milk they were made from. This strategy achieved excellent results, correctly classifying 77 (out of 80) validation samples. Eventually, since 36 (over 114) sheep ricotta whey cheeses were PDO products, a second classification problem, finalizing the discrimination of PDO and Non-PDO dairies, was faced. In this case, two classifiers were used, SPORT and soft independent modelling by class analogy (SIMCA). Both approaches provided more than satisfying results; in fact, SPORT properly assigned 63 (of 65) test samples, whereas the SIMCA model accepted 14 PDO individuals over 15 (93.3% sensitivity) and correctly rejected all the other samples (100.0% specificity). In conclusion, all the tested approaches resulted as suitable for the two fixed purposes. Eventually, variable importance in projection (VIP) analysis was used to understand which FAs characterize the different categories of ricotta. Among the 22 analyzed compounds, about 10 are considered the most relevant for the solution of the investigated problems.
    MeSH term(s) Female ; Cattle ; Sheep ; Animals ; Cheese/analysis ; Whey/chemistry ; Fatty Acids/analysis ; Chemometrics ; Whey Proteins/analysis ; Milk/chemistry ; Buffaloes ; Goats
    Chemical Substances Fatty Acids ; Whey Proteins
    Language English
    Publishing date 2022-10-31
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules27217401
    Database MEDical Literature Analysis and Retrieval System OnLINE

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