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Article ; Online: Filling knowledge gaps related to AmpC-dependent β-lactam resistance in Enterobacter cloacae

Isabel M. Barceló / María Escobar-Salom / Elena Jordana-Lluch / Gabriel Torrens / Antonio Oliver / Carlos Juan

Scientific Reports, Vol 14, Iss 1, Pp 1-

2024 Volume 11

Abstract: Abstract Enterobacter cloacae starred different pioneer studies that enabled the development of a widely accepted model for the peptidoglycan metabolism-linked regulation of intrinsic class C cephalosporinases, highly conserved in different Gram- ... ...

Abstract	Abstract Enterobacter cloacae starred different pioneer studies that enabled the development of a widely accepted model for the peptidoglycan metabolism-linked regulation of intrinsic class C cephalosporinases, highly conserved in different Gram-negatives. However, some mechanistic and fitness/virulence-related aspects of E. cloacae choromosomal AmpC-dependent resistance are not completely understood. The present study including knockout mutants, β-lactamase cloning, gene expression analysis, characterization of resistance phenotypes, and the Galleria mellonella infection model fills these gaps demonstrating that: (i) AmpC enzyme does not show any collateral activity impacting fitness/virulence; (ii) AmpC hyperproduction mediated by ampD inactivation does not entail any biological cost; (iii) alteration of peptidoglycan recycling alone or combined with AmpC hyperproduction causes no attenuation of E. cloacae virulence in contrast to other species; (iv) derepression of E. cloacae AmpC does not follow a stepwise dynamics linked to the sequential inactivation of AmpD amidase homologues as happens in Pseudomonas aeruginosa; (v) the enigmatic additional putative AmpC-type β-lactamase generally present in E. cloacae does not contribute to the classical cephalosporinase hyperproduction-based resistance, having a negligible impact on phenotypes even when hyperproduced from multicopy vector. This study reveals interesting particularities in the chromosomal AmpC-related behavior of E. cloacae that complete the knowledge on this top resistance mechanism.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2024-01-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Molecular Basis of AmpC β-Lactamase Induction by Avibactam in Pseudomonas aeruginosa

Silvia López-Argüello / María Montaner / Antonio Oliver / Bartolome Moya

International Journal of Molecular Sciences, Vol 22, Iss 3051, p

PBP Occupancy, Live Cell Binding Dynamics and Impact on Resistant Clinical Isolates Harboring PDC-X Variants

2021 Volume 3051

Abstract: Avibactam belongs to the new class of diazabicyclooctane β-lactamase inhibitors. Its inhibitory spectrum includes class A, C and D enzymes, including P. aeruginosa AmpC. Nonetheless, recent reports have revealed strain-dependent avibactam AmpC induction. ...

Abstract	Avibactam belongs to the new class of diazabicyclooctane β-lactamase inhibitors. Its inhibitory spectrum includes class A, C and D enzymes, including P. aeruginosa AmpC. Nonetheless, recent reports have revealed strain-dependent avibactam AmpC induction. In the present work, we wanted to assess the mechanistic basis underlying AmpC induction and determine if derepressed PDC-X mutated enzymes from ceftazidime/avibactam-resistant clinical isolates were further inducible. We determined avibactam concentrations that half-maximally inhibited (IC 50 ) bocillin FL binding. Inducer β-lactams were also studied as comparators. Live cells’ time-course penicillin-binding proteins (PBPs) occupancy of avibactam was studied. To assess the ampC induction capacity of avibactam and comparators, qRT-PCR was performed in wild-type PAO1, PBP4, triple PBP4, 5/6 and 7 knockout derivatives and two ceftazidime/avibactam-susceptible/resistant XDR clinical isolates belonging to the epidemic high-risk clone ST175. PBP4 inhibition was observed for avibactam and β-lactam comparators. Induction capacity was consistently correlated with PBP4 binding affinity. Outer membrane permeability-limited PBP4 binding was observed in the live cells’ assay. As expected, imipenem and cefoxitin showed strong induction in PAO1, especially for carbapenem; avibactam induction was conversely weaker. Overall, the inducer effect was less remarkable in ampC -derepressed mutants and nonetheless absent upon avibactam exposure in the clinical isolates harboring mutated AmpC variants and their parental strains.
Keywords	penicillin-binding proteins ; PBP ; avibactam ; AmpC induction ; PDC ; β-lactam resistance ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Language	English
Publishing date	2021-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Longitudinal Study of Dyadic Adjustment in a Sample of Spanish Fathers

Silvia Escribano PhD / Antonio Oliver-Roig PhD / Miguel Richart-Martínez PhD

American Journal of Men's Health, Vol

2020 Volume 14

Abstract: The objectives of this study were to examine the evolution of fathers’ long-term dyadic adjustment after the birth of a child and to analyze their evolution considering related factors. A total of 113 Spanish fathers with a mean age of 35.72 years ( SD = ...

Abstract	The objectives of this study were to examine the evolution of fathers’ long-term dyadic adjustment after the birth of a child and to analyze their evolution considering related factors. A total of 113 Spanish fathers with a mean age of 35.72 years ( SD = 3.84 years) participated. In general, there was a decline in the dyadic adjustment of the fathers until 6–12 months after childbirth, after which their level of adjustment remained stable until 13–24 months. We observed different patterns when analyzing the evolution by subgroups formed based on these different variables, previous experience of paternity, and anxiety. The intrinsic differences between fathers should also be considered because these differences can influence the way in which men face the parental process as well as the evolution of the quality of their relationship with their partner.
Keywords	Medicine ; R
Language	English
Publishing date	2020-10-01T00:00:00Z
Publisher	SAGE Publishing
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Visible‐Light‐Activated Molecular Machines Kill Fungi by Necrosis Following Mitochondrial Dysfunction and Calcium Overload

Ana L. Santos / Jacob L. Beckham / Dongdong Liu / Gang Li / Alexis vanVenrooy / Antonio Oliver / George P. Tegos / James M. Tour

Advanced Science, Vol 10, Iss 10, Pp n/a-n/a (2023)

2023

Abstract: Abstract Invasive fungal infections are a growing public health threat. As fungi become increasingly resistant to existing drugs, new antifungals are urgently needed. Here, it is reported that 405‐nm‐visible‐light‐activated synthetic molecular machines ( ... ...

Abstract	Abstract Invasive fungal infections are a growing public health threat. As fungi become increasingly resistant to existing drugs, new antifungals are urgently needed. Here, it is reported that 405‐nm‐visible‐light‐activated synthetic molecular machines (MMs) eliminate planktonic and biofilm fungal populations more effectively than conventional antifungals without resistance development. Mechanism‐of‐action studies show that MMs bind to fungal mitochondrial phospholipids. Upon visible light activation, rapid unidirectional drilling of MMs at ≈3 million cycles per second (MHz) results in mitochondrial dysfunction, calcium overload, and ultimately necrosis. Besides their direct antifungal effect, MMs synergize with conventional antifungals by impairing the activity of energy‐dependent efflux pumps. Finally, MMs potentiate standard antifungals both in vivo and in an ex vivo porcine model of onychomycosis, reducing the fungal burden associated with infection.
Keywords	antifungal ; fungal mitochondrial phospholipids ; molecular machines ; reduction of infection‐associated mortality and fungal burden ; visible light activation ; Science ; Q
Language	English
Publishing date	2023-04-01T00:00:00Z
Publisher	Wiley
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Book ; Thesis: Zwischen Rathaus, Milieu und Netzwerk

D'Antonio, Oliver

über die lokale Verankerung politischer Parteien

(Research)

2015

Title variant	Politische Parteien und lokale Verankerung
Author's details	Oliver D'Antonio
Series title	Research
Keywords	Kommunalpolitik ; Politische Kultur ; Partei ; Politisches Netzwerk ; Leipzig ; Frankfurt am Main
Language	German
Size	621 S.
Publisher	Springer VS
Publishing place	Wiesbaden
Document type	Book ; Thesis
Thesis / German Habilitation thesis	Univ., Diss. u.d.T. D'Antonio, Oliver: Politische Parteien und lokale Verankerung--Göttingen, 2014, eine empirische Fallstudie am Beispiel der Parteien FDP, Bündnis 90/Die Grünen und Die Linke in den Großstädten Frankfurt am Main und Leipzig
ISBN	3658072431 ; 9783658072438
Database	Former special subject collection: coastal and deep sea fishing

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Book ; Online ; Thesis: Zwischen Rathaus, Milieu und Netzwerk

D'Antonio, Oliver

über die lokale Verankerung politischer Parteien

2015

Abstract: Haben politische Parteien, wie oft unterstellt, den Kontakt zu den Bürgerinnen und Bürgern verloren? Und wie stellen ihre lokalen Einheiten eine Bindung zur Gesellschaft her? Oliver D’Antonio geht dieser Frage für die Parteien Bündnis 90/Die Grünen, Die ... ...

Author's details	von Oliver D’Antonio
Abstract	Haben politische Parteien, wie oft unterstellt, den Kontakt zu den Bürgerinnen und Bürgern verloren? Und wie stellen ihre lokalen Einheiten eine Bindung zur Gesellschaft her? Oliver D’Antonio geht dieser Frage für die Parteien Bündnis 90/Die Grünen, Die LINKE und FDP in Frankfurt am Main und Leipzig nach. Dazu führte er Gespräche mit Parteiaktiven und anderen lokalen Akteuren und begleitete das Parteileben vor Ort. Schließlich stellt er fest, dass die Parteien auf Basis unterschiedlicher Selbstverständnisse auch ihre Rolle in der lokalen Gesellschaft sehr verschiedenartig definieren und entsprechend handeln: Während manche Parteien fest in Milieus verankert sind, haben andere ausgreifende, aber fragile Netzwerke in die Stadtgesellschaften ausgeworfen und betonen ihre außerparlamentarische Aktivität. Wieder andere sehen sich eher als institutionelle Repräsentantinnen der Bürgerschaft in Rathaus und Verwaltung. Im Dreieck zwischen Rathaus, Milieu und Netzwerk konstituiert sich die lokale Verankerung politischer Parteien. Der Inhalt Interviews mit lokalen Parteiaktiven Aktivitäten der Parteibasis vor Ort Lokale Milieus und Netzwerke politischer Parteien Die Zielgruppen Dozierende und Studierende der Politikwissenschaft und Parteiensoziologie Parteimitglieder und KommunalpolitikerInnen Der Autor Dr. Oliver D’Antonio ist wissenschaftlicher Mitarbeiter am Institut für Demokratieforschung an der Georg-August-Universität Göttingen
Keywords	Social sciences
Language	German
Size	Online-Ressource (XVI, 605 S), online resource
Publisher	Springer VS
Publishing place	Wiesbaden
Document type	Book ; Online ; Thesis
Thesis / German Habilitation thesis	Georg-August-Univ., Diss.--Göttingen, 2014
Note	Includes bibliographical references
ISBN	9783658072438 ; 9783658072445 ; 3658072431 ; 365807244X
DOI	10.1007/978-3-658-07244-5
Database	Former special subject collection: coastal and deep sea fishing

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Book ; Online ; Thesis: Zwischen Rathaus, Milieu und Netzwerk

D'Antonio, Oliver

über die lokale Verankerung politischer Parteien

2015

Author's details	von Oliver D’Antonio
Abstract	Haben politische Parteien, wie oft unterstellt, den Kontakt zu den Bürgerinnen und Bürgern verloren? Und wie stellen ihre lokalen Einheiten eine Bindung zur Gesellschaft her? Oliver D’Antonio geht dieser Frage für die Parteien Bündnis 90/Die Grünen, Die LINKE und FDP in Frankfurt am Main und Leipzig nach. Dazu führte er Gespräche mit Parteiaktiven und anderen lokalen Akteuren und begleitete das Parteileben vor Ort. Schließlich stellt er fest, dass die Parteien auf Basis unterschiedlicher Selbstverständnisse auch ihre Rolle in der lokalen Gesellschaft sehr verschiedenartig definieren und entsprechend handeln: Während manche Parteien fest in Milieus verankert sind, haben andere ausgreifende, aber fragile Netzwerke in die Stadtgesellschaften ausgeworfen und betonen ihre außerparlamentarische Aktivität. Wieder andere sehen sich eher als institutionelle Repräsentantinnen der Bürgerschaft in Rathaus und Verwaltung. Im Dreieck zwischen Rathaus, Milieu und Netzwerk konstituiert sich die lokale Verankerung politischer Parteien. Der Inhalt Interviews mit lokalen Parteiaktiven Aktivitäten der Parteibasis vor Ort Lokale Milieus und Netzwerke politischer Parteien Die Zielgruppen Dozierende und Studierende der Politikwissenschaft und Parteiensoziologie Parteimitglieder und KommunalpolitikerInnen Der Autor Dr. Oliver D’Antonio ist wissenschaftlicher Mitarbeiter am Institut für Demokratieforschung an der Georg-August-Universität Göttingen
Keywords	Social sciences
Language	German
Size	Online-Ressource (XVI, 605 S), online resource
Publisher	Springer VS
Publishing place	Wiesbaden
Document type	Book ; Online ; Thesis
Thesis / German Habilitation thesis	Georg-August-Univ., Diss.--Göttingen, 2014
ISBN	9783658072438 ; 9783658072445 ; 3658072431 ; 365807244X
DOI	10.1007/978-3-658-07244-5
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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Book ; Thesis: Zwischen Rathaus, Milieu und Netzwerk

D'Antonio, Oliver

über die lokale Verankerung politischer Parteien

(Research)

2015

Title variant	Politische Parteien und lokale Verankerung
Author's details	Oliver D'Antonio
Series title	Research
Keywords	Kommunalpolitik ; Politische Kultur ; Partei ; Politisches Netzwerk ; Leipzig ; Frankfurt am Main
Language	German
Size	621 S.
Publisher	Springer VS
Publishing place	Wiesbaden
Document type	Book ; Thesis
Thesis / German Habilitation thesis	Univ., Diss. u.d.T. D'Antonio, Oliver: Politische Parteien und lokale Verankerung--Göttingen, 2014, eine empirische Fallstudie am Beispiel der Parteien FDP, Bündnis 90/Die Grünen und Die Linke in den Großstädten Frankfurt am Main und Leipzig
ISBN	3658072431 ; 9783658072438
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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Inter-library loan at ZB MED

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Article ; Online: Effects of Inhaled Corticosteroids on the Innate Immunological Response to Pseudomonas aeruginosa Infection in Patients with COPD

Noemi Cerón-Pisa / Hanaa Shafiek / Aina Martín-Medina / Javier Verdú / Elena Jordana-Lluch / Maria Escobar-Salom / Isabel M. Barceló / Carla López-Causapé / Antonio Oliver / Carlos Juan / Amanda Iglesias / Borja G. Cosío

International Journal of Molecular Sciences, Vol 23, Iss 8127, p

2022 Volume 8127

Abstract: Inhaled corticosteroids (ICS) use is associated with an increased risk of Pseudomonas aeruginosa (PA) infection in patients with COPD. We aimed to evaluate the effects of ICS on alveolar macrophages in response to PA in COPD patients with and without ... ...

Abstract	Inhaled corticosteroids (ICS) use is associated with an increased risk of Pseudomonas aeruginosa (PA) infection in patients with COPD. We aimed to evaluate the effects of ICS on alveolar macrophages in response to PA in COPD patients with and without baseline ICS treatment (COPD and COPD + ICS, respectively) as well as smoker and nonsmoker controls. To do so, cells were infected with PA and cotreated with budesonide (BUD) or fluticasone propionate (FLU). The analysis of NF-κB and c-jun activity revealed a significant increase in both factors in response to PA cotreated with BUD/FLU in smokers but not in COPD or COPD + ICS patients when compared with PA infection alone. The expression of Toll-like receptor 2 (TLR2) and the transcription factor c-jun were induced upon PA infection in nonsmokers only. Moreover, in the smoker and COPD groups, there was a significant increase in TLR2 and a decrease in c-jun expression when treated with BUD/FLU after PA infection, which were not observed in COPD + ICS patients. Therefore, the chronic use of ICS seemingly makes the macrophages tolerant to BUD/FLU stimulation compared with those from patients not treated with ICS, promoting an impaired recognition of PA and activity of alveolar macrophages in terms of altered expression of TLR2 and cytokine production, which could explain the increased risk of PA infection in COPD patients under ICS treatment.
Keywords	corticosteroids ; COPD ; Pseudomonas aeruginosa ; inflammatory cytokines ; Toll-like receptors ; transcription factors ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	616 ; 610
Language	English
Publishing date	2022-07-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Book ; Online: Zwischen Rathaus, Milieu und Netzwerk

D'Antonio, Oliver

Über die lokale Verankerung politischer Parteien

2014

Abstract: Haben politische Parteien, wie oft unterstellt, den Kontakt zu den Bürgerinnen und Bürgern verloren? Und wie stellen ihre lokalen Einheiten eine Bindung zur Gesellschaft her? Oliver D'Antonio geht dieser Frage für die Parteien Bündnis 90/Die Grünen, Die ... ...

Abstract	Haben politische Parteien, wie oft unterstellt, den Kontakt zu den Bürgerinnen und Bürgern verloren? Und wie stellen ihre lokalen Einheiten eine Bindung zur Gesellschaft her? Oliver D'Antonio geht dieser Frage für die Parteien Bündnis 90/Die Grünen, Die LINKE und FDP in Frankfurt am Main und Leipzig nach. Dazu führte er Gespräche mit Parteiaktiven und anderen lokalen Akteuren und begleitete das Parteileben vor Ort. Schließlich stellt er fest, dass die Parteien auf Basis unterschiedlicher Selbstverständnisse auch ihre Rolle in der lokalen Gesellschaft sehr verschiedenartig definieren und entsprechend handeln: Während manche Parteien fest in Milieus verankert sind, haben andere ausgreifende, aber fragile Netzwerke in die Stadtgesellschaften ausgeworfen und betonen ihre außerparlamentarische Aktivität. Wieder andere sehen sich eher als institutionelle Repräsentantinnen der Bürgerschaft in Rathaus und Verwaltung. Im Dreieck zwischen Rathaus, Milieu und Netzwerk konstituiert sich die lokale Verankerung politischer Parteien. Dr. Oliver D'Antonio ist wissenschaftlicher Mitarbeiter am Institut für Demokratieforschung an der Georg-August-Universität Göttingen.
Language	German
Size	Online-Ressource
Edition	1. Aufl.
Publisher	Springer VS
Publishing place	s.l.
Document type	Book ; Online
Note	Description based upon print version of record
ISBN	3658072431 ; 9783658072438
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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