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  1. Article ; Online: Molecular and physiological characterization of Fusarium strains associated with different diseases in date palm.

    Amgad A Saleh / Anwar H Sharafaddin / Mahmoud H El Komy / Yasser E Ibrahim / Younis K Hamad

    PLoS ONE, Vol 16, Iss 7, p e

    2021  Volume 0254170

    Abstract: Several species of Fusarium cause serious diseases in date palm worldwide. In the present work, 14 SSR markers were used to assess the genetic variation of Fusarium strains isolated from diseased trees in Saudi Arabia. We also studied the effect of ... ...

    Abstract Several species of Fusarium cause serious diseases in date palm worldwide. In the present work, 14 SSR markers were used to assess the genetic variation of Fusarium strains isolated from diseased trees in Saudi Arabia. We also studied the effect of different temperatures on mycelial growth of these strains. The pathogenicity of four strains of F. proliferatum was also evaluated on local date palm cultivars. Eleven SSR markers amplified a total of 57 scorable alleles from Fusarium strains. Phylogenetic analysis showed that F. proliferatum strains grouped in one clade with 95% bootstrap value. Within F. proliferatum clade, 14 SSR genotypes were identified, 9 of them were singleton. Four out of the five multi-individual SSR genotypes contained strains isolated from more than one location. Most F. solani strains grouped in one clade with 95% bootstrap value. Overall, the SSR markers previously developed for F. verticillioides and F. oxysporum were very useful in assessing the genetic diversity and confirming the identity of Saudi Fusarium strains. The results from the temperature study showed significant differences in mycelial growth of Fusarium strains at different temperatures tested. The highest average radial growth for Fusarium strains was observed at 25°C, irrespective of species. The four F. proliferatum strains showed significant differences in their pathogenicity on date palm cultivars. It is anticipated that the assessment of genetic diversity, effect of temperature on hyphal growth and pathogenicity of potent pathogenic Fusarium strains recovered from date palm-growing locations in Saudi Arabia can help in effectively controlling these pathogens.
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Prevalence of Escherichia coli strains resistance to antibiotics in wound infections and raw milk

    Naiyf S. Alharbi / Jamal M. Khaled / Shine Kadaikunnan / Ahmed S. Alobaidi / Anwar H. Sharafaddin / Sami A. Alyahya / Taghreed N. Almanaa / Mohammad A. Alsughayier / Muhammed R. Shehu

    Saudi Journal of Biological Sciences, Vol 26, Iss 7, Pp 1557-

    2019  Volume 1562

    Abstract: Antibiotic-resistant Escherichia coli strains including extended-spectrum β-lactamase (ESBL) isolates are globally widespread in medical, food, and environmental sources. Some of these strains are considered the most pathogenic bacteria in humans. The ... ...

    Abstract Antibiotic-resistant Escherichia coli strains including extended-spectrum β-lactamase (ESBL) isolates are globally widespread in medical, food, and environmental sources. Some of these strains are considered the most pathogenic bacteria in humans. The present work examined the predominance of antibiotic resistance in E. coli strains in wound infections comparing with E. coli strains isolated from a raw milk as a potential source of those strains. The wound infections included abdomen, anus, arm, back, buttock, chest, foot, hand, head, leg, lung, mouth, neck, penis, thigh, toe, and vagina infections. In total, 161 and 153 isolates identified as E. coli were obtained from wound infections and raw milk, respectively. A Vitek 2 system innovated by bioMérieux, France was applied to perform the identification and susceptibility tests. The E. coli isolates that have ability to produce ESBL were detected by an ESBL panel and NO45 card (bioMérieux). Over half of the E. coli were from abdomen, back, and buttock wound infections. More than 50%of the E. coli isolates obtained from wound infections were resistant to cefazolin, ampicillin, cefuroxime, ciprofloxacin, mezlocillin, moxifloxacin, piperacillin, and tetracycline; 70% of the isolates from wound infections and 0% of the isolates from raw milk were E. coli isolates produced ESBL. The data showed that the strains resistance to multi-antibiotic and produced ESBL are more widespread among wound infections than in raw milk. Keywords: Escherichia coli, Wound infections, Antibiotics, Raw milk
    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2019-11-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article: Fusarium species associated with date palm in Saudi Arabia

    Saleh, AmgadA / Anwar H. Sharafaddin / Mahmoud H. El_Komy / Yasser E. Ibrahim / Younis K. Hamad / Younis Y. Molan

    European journal of plant pathology. 2017 June, v. 148, no. 2

    2017  

    Abstract: Fusarium is one of the most destructive fungal genera whose members cause many diseases on plants, animals, and humans. Moreover, many Fusarium species secrete mycotoxins (e.g. trichothecenes and fumonisins) that are toxic to humans and animals. Fusarium ...

    Abstract Fusarium is one of the most destructive fungal genera whose members cause many diseases on plants, animals, and humans. Moreover, many Fusarium species secrete mycotoxins (e.g. trichothecenes and fumonisins) that are toxic to humans and animals. Fusarium isolates from date palm trees showing disease symptoms, e.g. chlorosis, necrosis and whitening, were collected from seven regions across Saudi Arabia. After single-sporing, the fungal strains were morphologically characterized. To confirm the identity of morphologically characterized Fusarium strains, three nuclear loci, two partial genes of translation elongation factor 1 α (tef1α) and β-tubulin (tub2), and the rDNA-ITS region, were amplified and sequenced. Of the 70 Fusarium strains, 70 % were identified as F. proliferatum that were recovered from six regions across Saudi Arabia. Fusarium solani (13 %), as well as one strain each of the following species: F. brachygibbosum, F. oxysporum, and F. verticillioides were also recovered. In addition, five Fusarium-like strains were recognized as Sarocladium kiliense by DNA-based data. The preliminary in vitro pathogenicity results showed that F. proliferatum had the highest colonization abilities on date palm leaflets, followed by F. solani. Although F. oxysporum f. sp. albedinis is the most serious date palm pathogen, F. proliferatum and F. solani are becoming serious pathogens and efforts should be made to restrict and control them. In addition, the potential toxin risks of strains belonging to F. proliferatum should be evaluated.
    Keywords Fusarium solani ; Phoenix dactylifera ; Sarocladium ; chlorosis ; fumonisins ; fungi ; genes ; humans ; loci ; necrosis ; pathogenicity ; pathogens ; plants (botany) ; risk ; toxicity ; trichothecenes ; tubulin ; Saudi Arabia
    Language English
    Dates of publication 2017-06
    Size p. 367-377.
    Publishing place Springer Netherlands
    Document type Article
    ZDB-ID 1196520-4
    ISSN 0929-1873
    ISSN 0929-1873
    DOI 10.1007/s10658-016-1095-3
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 1151: Show issues

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  4. Article: Brevibacillus laterosporus isolated from the digestive tract of honeybees has high antimicrobial activity and promotes growth and productivity of honeybee’s colonies

    Khaled, JamalM / Fahd A. Al-Mekhlafi / Ramzi A. Mothana / Naiyf S. Alharbi / Khalid E. Alzaharni / Anwar H. Sharafaddin / Shine Kadaikunnan / Ahmed S. Alobaidi / Noofal I. Bayaqoob / Marimuthu Govindarajan / Giovanni Benelli

    Environmental science and pollution research international. 2018 Apr., v. 25, no. 11

    2018  

    Abstract: The development of novel antimicrobial drugs, as well as the discovery of novel compounds able to promote honeybee’s growth, represents major challenges for modern entomology. The main aim of this study was to investigate whether Brevibacillus ... ...

    Abstract The development of novel antimicrobial drugs, as well as the discovery of novel compounds able to promote honeybee’s growth, represents major challenges for modern entomology. The main aim of this study was to investigate whether Brevibacillus laterosporus isolated from the digestive tract of Saudi honeybees, Apis mellifera, was able to stimulate colony strength parameters of honeybees and to evaluate its ability to produce antimicrobial agents. Honeybees were collected in Dirab, Riyadh Region, Saudi Arabia, and microorganisms were isolated and identified by 16S ribosomal RNA analysis. Microscopic identification of the microorganism in its native state was facilitated by atomic force microscopy at high-resolution imaging. Active biological compounds were produced by submerged fermentation with B. laterosporus. The fermented broth was subjected to extraction and purification, and then semi-pure compounds were analyzed by gas chromatography–mass spectrometry. The effectiveness of the crude extract and semi-pure compounds as antimicrobial agents was evaluated by susceptibility assays. More than 22% of the microorganisms isolated from the digestive tract of healthy honeybees have been identified as B. laterosporus, this kind of species has a unique shape and morphological structure. The cyclic dipeptide cyclo(Leu-Pro) produced by B. laterosporus showed biological activity against several pathogenic microorganisms. Furthermore, the total counts of workers, closed brood, and open brood, as well as the production of bee pollen and honey, were better in honeybees treated with a B. laterosporus suspension. The data indicated that the B. laterosporus strain isolated from a healthy honeybee might be a novel probiotic and a producer of important biological compounds.
    Keywords Apis mellifera ; Brevibacillus laterosporus ; anti-infective agents ; antimicrobial properties ; atomic force microscopy ; bee pollen ; digestive tract ; drugs ; entomology ; gas chromatography-mass spectrometry ; honey ; honey bees ; image analysis ; probiotics ; ribosomal RNA ; submerged fermentation ; Saudi Arabia
    Language English
    Dates of publication 2018-04
    Size p. 10447-10455.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    ZDB-ID 1178791-0
    ISSN 1614-7499 ; 0944-1344
    ISSN (online) 1614-7499
    ISSN 0944-1344
    DOI 10.1007/s11356-017-0071-6
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 5915: Show issues

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  5. Article: Correction to: Brevibacillus laterosporus isolated from the digestive tract of honeybees has high antimicrobial activity and promotes growth and productivity of honeybee’s colonies

    Khaled, Jamal M / Ahmed S. Alobaidi / Anwar H. Sharafaddin / Fahd A. Al-Mekhlafi / Giovanni Benelli / Khalid E. Alzaharni / Marimuthu Govindarajan / Naiyf S. Alharbi / Noofal I. Bayaqoob / Ramzi A. Mothana / Shine Kadaikunnan

    Environmental science and pollution research international. 2018 Aug., v. 25, no. 24

    2018  

    Abstract: The original publication of this paper contains a mistake. The correct affiliation no. 3 is shown in this paper. ...

    Abstract The original publication of this paper contains a mistake. The correct affiliation no. 3 is shown in this paper.
    Keywords antimicrobial properties ; Brevibacillus laterosporus ; digestive tract ; honey bees
    Language English
    Dates of publication 2018-08
    Size p. 24516.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    Note Published Erratum
    ZDB-ID 1178791-0
    ISSN 1614-7499 ; 0944-1344
    ISSN (online) 1614-7499
    ISSN 0944-1344
    DOI 10.1007/s11356-018-2721-8
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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