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  1. Article ; Online: Molecular detection of canine respiratory pathogens between 2017 and 2018 in Japan.

    Matsuu, Aya / Yabuki, Mihoko / Aoki, Emiko / Iwahana, Michio

    The Journal of veterinary medical science

    2020  Volume 82, Issue 6, Page(s) 690–694

    Abstract: A molecular survey was conducted to understand recent distribution of pathogens associated with canine infectious respiratory disease (CIRD) in Japan. Nasal and/or pharyngeal swabs were collected from asymptomatic dogs and those with CIRD, living in ... ...

    Abstract A molecular survey was conducted to understand recent distribution of pathogens associated with canine infectious respiratory disease (CIRD) in Japan. Nasal and/or pharyngeal swabs were collected from asymptomatic dogs and those with CIRD, living in private house or in kennels. PCR-based examination was conducted for detecting nine pathogens. Among private household dogs, 50.8% with CIRD, 11.1% with respiratory disease other than CIRD, and 4.3% asymptomatic were positive for more than one pathogen, whereas in kennel-housed dogs, 42.9% with CIRD and 27.3% asymptomatic were positive. Bordetella bronchiseptica was most frequently detected, followed by canine herpesvirus 1, canine parainfluenza virus, canine pneumovirus, Mycoplasma cynos, and canine adenovirus type 2. In kennel environment, asymptomatic dogs might act as reservoirs carrying the respiratory pathogens.
    MeSH term(s) Animals ; Bordetella bronchiseptica ; Dog Diseases/diagnosis ; Dog Diseases/microbiology ; Dog Diseases/virology ; Dogs ; Female ; Japan ; Male ; Molecular Diagnostic Techniques/veterinary ; Respiratory Tract Infections/diagnosis ; Respiratory Tract Infections/microbiology ; Respiratory Tract Infections/veterinary ; Respiratory Tract Infections/virology
    Keywords covid19
    Language English
    Publishing date 2020-04-07
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 1071753-5
    ISSN 1347-7439 ; 0916-7250
    ISSN (online) 1347-7439
    ISSN 0916-7250
    DOI 10.1292/jvms.20-0017
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Development of the differential detection reverse transcription loop-mediated isothermal amplification method for Barley yellow mosaic virus or Japanese soil-borne wheat mosaic virus from barley.

    Fukuta, Shiro / Kato, Shuhei / Suzuki, Ryoji / Arakawa, Mizuho / Goto, Koji / Maejima, Hidekazu / Nagamine, Takashi / Aoki, Emiko

    Letters in applied microbiology

    2023  Volume 76, Issue 2

    Abstract: A differential detection reverse transcription loop-mediated isothermal amplification (DD-RT-LAMP) method was developed to detect either Barley yellow mosaic virus (BaYMV) or Japanese soil-borne wheat mosaic virus (JSBWMV) simultaneously. Both primer ... ...

    Abstract A differential detection reverse transcription loop-mediated isothermal amplification (DD-RT-LAMP) method was developed to detect either Barley yellow mosaic virus (BaYMV) or Japanese soil-borne wheat mosaic virus (JSBWMV) simultaneously. Both primer sets, which recognized either BaYMV or JSBWMV genomic RNA, amplified DNA more efficiently at 65°C using an isothermal DNA amplification and fluorescence detection device. Furthermore, these primer sets showed unique annealing curves. The peak annealing temperatures of BaYMV and JSBWMV amplification products using specific primer sets were 86.9°C-87.7°C and 84.5°C-85.0°C, respectively, and were clearly distinguishable during an annealing step following the isothermal amplification, monitored using a fluorescence detection device. In the field samples of barley (Hordeum vulgare L.) tested, BaYMV or JSBWMV were detected by DD-RT-LAMP, and the detection results of DD-RT-LAMP were correspondent with the results of reverse transcription-PCR.
    MeSH term(s) Hordeum/virology ; Nucleic Acid Amplification Techniques/methods ; Reverse Transcription ; Sensitivity and Specificity ; Plant Diseases/virology ; Plant Viruses/isolation & purification
    Language English
    Publishing date 2023-01-24
    Publishing country England
    Document type Journal Article
    ZDB-ID 632584-1
    ISSN 1472-765X ; 0266-8254
    ISSN (online) 1472-765X
    ISSN 0266-8254
    DOI 10.1093/lambio/ovac065
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Molecular detection of canine respiratory pathogens between 2017 and 2018 in Japan

    Matsuu, Aya / Yabuki, Mihoko / Aoki, Emiko / Iwahana, Michio

    J Vet Med Sci

    Abstract: A molecular survey was conducted to understand recent distribution of pathogens associated with canine infectious respiratory disease (CIRD) in Japan. Nasal and/or pharyngeal swabs were collected from asymptomatic dogs and those with CIRD, living in ... ...

    Abstract A molecular survey was conducted to understand recent distribution of pathogens associated with canine infectious respiratory disease (CIRD) in Japan. Nasal and/or pharyngeal swabs were collected from asymptomatic dogs and those with CIRD, living in private house or in kennels. PCR-based examination was conducted for detecting nine pathogens. Among private household dogs, 50.8% with CIRD, 11.1% with respiratory disease other than CIRD, and 4.3% asymptomatic were positive for more than one pathogen, whereas in kennel-housed dogs, 42.9% with CIRD and 27.3% asymptomatic were positive. Bordetella bronchiseptica was most frequently detected, followed by canine herpesvirus 1, canine parainfluenza virus, canine pneumovirus, Mycoplasma cynos, and canine adenovirus type 2. In kennel environment, asymptomatic dogs might act as reservoirs carrying the respiratory pathogens.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #664667
    Database COVID19

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  4. Article ; Online: Investigation of the possibility of using an augmented reality-based endotracheal aspiration simulation tool for nursing education.

    Arakida, Mikako / Takahashi, Tokiichiro / Matsuda, Yuko / Yamabe, Yuta / Miyamoto, Chizuko / Aoki, Emiko / Takahashi, Sawako / Ando, Mizuho / Moriya, Tomoaki / Kodama, Shuhei / Yamashita, Ruriko / Igarashi, Takahiro / Tanizaki, Keito / Uchida, Kenta

    Japan journal of nursing science : JJNS

    2023  Volume 21, Issue 2, Page(s) e12573

    Abstract: Aim: We developed an augmented reality technology-based endotracheal aspiration simulation tool (the AR tool) consisting of three modes: a mode for nursing students to learn endotracheal aspiration by themselves (learning mode); a mode for repeated ... ...

    Abstract Aim: We developed an augmented reality technology-based endotracheal aspiration simulation tool (the AR tool) consisting of three modes: a mode for nursing students to learn endotracheal aspiration by themselves (learning mode); a mode for repeated practice (practice mode); and a mode for confirmation testing (test mode). This study aimed to compare the learning outcomes of the AR tool with traditional training mannequins and identify potential uses and improvements of the AR tool.
    Methods: We invited students, and faculty members from the three universities who agreed to cooperate in conducting this study. Fifty-four students and nine faculty members agreed to participate in this study. The students were divided into two groups. One group was to study with the AR tool and the other group was to study with the traditional half-body training mannequin for suction. The students in both groups were asked to demonstrate endotracheal aspiration on another full-body type training mannequin which could be used as a patient with a tracheostomy (Skill test). Group interviews with faculty members focused on the topic of the AR tool's potential use in nursing education and improvement needed.
    Results: There was little significant difference in the skill test results of endotracheal aspiration of students in both groups. The students and faculty members both expressed an interest in the AR tool. They said it was suitable for self-study of endotracheal aspiration.
    Conclusions: This AR tool is an effective teaching tool for learning the sequence of endotracheal suctioning, although there are some areas that need improvement.
    MeSH term(s) Humans ; Augmented Reality ; Education, Nursing ; Manikins ; Learning ; Students ; Students, Nursing
    Language English
    Publishing date 2023-12-12
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 2156618-5
    ISSN 1742-7924 ; 1742-7932
    ISSN (online) 1742-7924
    ISSN 1742-7932
    DOI 10.1111/jjns.12573
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: SURVEILLANCE FOR ANTIBODIES AGAINST SIX CANINE VIRUSES IN WILD RACCOONS (PROCYON LOTOR) IN JAPAN.

    Aoki, Emiko / Soma, Takehisa / Yokoyama, Mayumi / Matsubayashi, Makoto / Sasai, Kazumi

    Journal of wildlife diseases

    2017  Volume 53, Issue 4, Page(s) 761–768

    Abstract: Raccoons (Procyon lotor) are found worldwide. They are frequently seen in crowded inner cities as well as in forests or wooded areas, often living in proximity to humans and their pets. We examined sera from 100 wild raccoons in Japan for antibodies to ... ...

    Abstract Raccoons (Procyon lotor) are found worldwide. They are frequently seen in crowded inner cities as well as in forests or wooded areas, often living in proximity to humans and their pets. We examined sera from 100 wild raccoons in Japan for antibodies to six canine viruses with veterinary significance to assess their potential as reservoirs. We also aimed to understand the distribution of potentially infected wildlife. We found that 7% of samples were seropositive for canine distemper virus (CDV), 10% for canine parvovirus type 2, 2% for canine adenovirus type 1, 6% for canine adenovirus type 2, and 7% for canine coronavirus. No samples were found to be seropositive for canine parainfluenza virus. Seropositivity rates for canine distemper virus and canine parvovirus type 2 were significantly different between areas, and younger raccoons (<1 yr old) were more frequently seropositive than older raccoons. Because raccoons belong to the suborder Caniformia, similar to dogs (Canis lupus familiaris), our results suggest that they can act as reservoirs for some of these important canine viruses and might be involved in viral transmission. Further study should include isolation and analysis of canine viruses in wild raccoons from a wider area.
    Keywords covid19
    Language English
    Publishing date 2017-10
    Publishing country United States
    Document type Journal Article
    ZDB-ID 410709-3
    ISSN 1943-3700 ; 0090-3558
    ISSN (online) 1943-3700
    ISSN 0090-3558
    DOI 10.7589/2016-11-253
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: [Examination of the effects of a role-playing training program for the improvement of planning and management skills of public health nurses].

    Arakida, Mikako / Matsuda, Yuko / Aoki, Emiko / Takenaka, Kanako / Yamashita, Ruriko / Rokuro, Keiko / Yamazaki, Itsuko / Machida, Keiko / Funakawa, Yuka

    Nihon koshu eisei zasshi] Japanese journal of public health

    2020  Volume 67, Issue 12, Page(s) 881–891

    Abstract: Objectives The Japan Health Insurance Association (JHIA) conducts training in each branch facility to improve the ability of public health nurses (PHNs). The headquarters of the PHNs of JHIA and the researchers conducted a training program for leaders of ...

    Abstract Objectives The Japan Health Insurance Association (JHIA) conducts training in each branch facility to improve the ability of public health nurses (PHNs). The headquarters of the PHNs of JHIA and the researchers conducted a training program for leaders of PHNs at each JHIA branch. The goal of the program was to create a training plan using role-play to acquire facilitation skills. This study aimed to examine the effects of training.Methods The study was designed in accordance with the Instructional Designs. The training goals were as follows: (1) understanding the role of facilitation and the facilitator in the debriefing session after the role-play, (2) understanding facilitation techniques, (3) being confident in performing as facilitators in the debriefing session; and (4) conducting the debriefing sessions using facilitation skills. The evaluation of the training was based on the Kirkpatrick model from the viewpoint of confidence in using facilitation, knowledge of facilitation, and conduct of training and utilization of facilitation technology. Questionnaire evaluations were conducted three times before the training, immediately after the training, and three months after the training. In August 2016, 4.5 hours of training were conducted in one day.Results There were 79 participants in the training group. The mean points of knowledge and confidence were 2.6-3.6 before training, 6.3-7.9 after training, and 6.0-6.9 at 3 months after training. The participants rated their interest in three questions of the training as high as 8.1-8.6. In addition, 64.6% of participants held a role-play session at each branch within three months of the initial training. In the role-play session planned by the participants, the practitioners implemented the explanation of the purpose and the rules at each branch 96.1% and 98.0%, respectively. Participants who had attended facilitation training prior to our program scored higher points of knowledge and confidence before and after three months. Three months after the training, 79 participants responded to the question of the role of the facilitator in role-playing. The descriptions were categorized into "opinions on the basics and planning of role-play training" and "opinions on the roles in retrospectives."Conclusion The participants evaluated the training contents and the materials used as appropriate, and their knowledge and confidence in facilitation improved after the training. To maintain and improve facilitation skills, the educational system needs to implement training using role-playing at each branch consistently.
    MeSH term(s) Clinical Competence ; Education, Nursing/methods ; Education, Public Health Professional/methods ; Government Programs ; Insurance, Health/organization & administration ; Japan ; Knowledge ; Nurses, Public Health/education ; Nurses, Public Health/psychology ; Professional Role ; Program Evaluation ; Quality Improvement ; Quality of Health Care ; Self Concept ; Time Factors
    Language Japanese
    Publishing date 2020-12-31
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 45044-3
    ISSN 0546-1766
    ISSN 0546-1766
    DOI 10.11236/jph.67.12_881
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Surveillance for antibodies against six canine viruses in wild raccoons (procyon lotor) in japan

    Aoki, Emiko / Kazumi Sasai / Makoto Matsubayashi / Mayumi Yokoyama / Takehisa Soma

    Journal of wildlife diseases. 2017 Oct., v. 53, no. 4

    2017  

    Abstract: Raccoons (Procyon lotor) are found worldwide. They are frequently seen in crowded inner cities as well as in forests or wooded areas, often living in proximity to humans and their pets. We examined sera from 100 wild raccoons in Japan for antibodies to ... ...

    Abstract Raccoons (Procyon lotor) are found worldwide. They are frequently seen in crowded inner cities as well as in forests or wooded areas, often living in proximity to humans and their pets. We examined sera from 100 wild raccoons in Japan for antibodies to six canine viruses with veterinary significance to assess their potential as reservoirs. We also aimed to understand the distribution of potentially infected wildlife. We found that 7% of samples were seropositive for canine distemper virus (CDV), 10% for canine parvovirus type 2, 2% for canine adenovirus type 1, 6% for canine adenovirus type 2, and 7% for canine coronavirus. No samples were found to be seropositive for canine parainfluenza virus. Seropositivity rates for canine distemper virus and canine parvovirus type 2 were significantly different between areas, and younger raccoons (<1 yr old) were more frequently seropositive than older raccoons. Because raccoons belong to the suborder Caniformia, similar to dogs (Canis lupus familiaris), our results suggest that they can act as reservoirs for some of these important canine viruses and might be involved in viral transmission. Further study should include isolation and analysis of canine viruses in wild raccoons from a wider area.
    Keywords antibodies ; Canine adenovirus A ; Canine coronavirus ; Canine distemper virus ; Canis lupus ; Carnivore protoparvovirus 1 ; cities ; dogs ; forests ; monitoring ; pets ; Procyon lotor ; seroprevalence ; virus transmission ; viruses ; wildlife ; Japan ; covid19
    Language English
    Dates of publication 2017-10
    Size p. 761-768.
    Publishing place Wildife Disease Association
    Document type Article
    ZDB-ID 410709-3
    ISSN 1943-3700 ; 0090-3558
    ISSN (online) 1943-3700
    ISSN 0090-3558
    DOI 10.7589/2016-11-253
    Database NAL-Catalogue (AGRICOLA)

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  8. Article: Development of Barley Cultivars with Resistance to Scald (Rhynchosporium secalis (Oud.) Davis) in Japan

    Aoki, Emiko / Baba, Takahide / Yamaguchi, Osamu / Ito, Seiji / Moriwaki, Jouji

    Japan agricultural research quarterly

    2011  Volume 45, Issue 4, Page(s) 349

    Document type Article
    ZDB-ID 407386-1
    ISSN 0021-3551
    Database Current Contents Nutrition, Environment, Agriculture

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