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  1. Article ; Online: Identificación de marcadores de transición epitelio-mesénquima (TEM) por inmunohistoquímica en osteosarcoma pediátrico y asociación con desenlaces clínicos.

    Fonseca, Eileen V / Olaya, Natalia / Patifto, Esteban F / Aponte, Nelson H / Linares Ballesteros, Adriana

    Andes pediatrica : revista Chilena de pediatria

    2023  Volume 93, Issue 6, Page(s) 868–877

    Abstract: The epithelial-mesenchymal transition (EMT) is the ability of epithelial and mesenchymal cells to exchange phenotypes transiently. Its identification in carcinomatous cells has been associated with aggressive clinical phenotypes. In sarcomas, this ... ...

    Title translation Identification of epithelial-mesenchymal transition markers (EMT) by immunohistochemistry in pediatric osteosarcoma and association with clinical outcomes.
    Abstract The epithelial-mesenchymal transition (EMT) is the ability of epithelial and mesenchymal cells to exchange phenotypes transiently. Its identification in carcinomatous cells has been associated with aggressive clinical phenotypes. In sarcomas, this ability is under study.
    Objective: to evaluate the expression of two transcription factors involved in EMT by immunohistochemistry in pediatric osteosarcoma and its association with clinical outcomes.
    Patients and method: A retrospective cohort study in children under 18 years of age with osteosarcoma diagnosis. Immunohistochemistry was performed for Snail and Twist-1 expressions from samples collected at the time of diagnosis. Correlations between immunohistochemistry and the clinical outcomes and overall survival were performed.
    Results: 53 patients were included. There were 26 positive cytoplasmic cases (49.1%) in Snail expression and were correlated with the presence of multiple metastases (p = 0.02) and distant bone metastases (p = 0.01). On the other hand, 45 cases (84.9%) were positive in Twist-1 expression in the nuclear location, showing no association with the analyzed clinical variables.
    Conclusions: Snail and Twist-1 were frequently expressed in pediatric cases of osteosarcoma. Cytoplasmic Snail was correlated with the presence of multiple metastatic disease and distant bone metastases. The positivity of both markers suggests the activation of these proteins as regulators of EMT events in this tumor, suggesting a role in the phenomena related to the clinical presentation of the disease.
    MeSH term(s) Humans ; Child ; Adolescent ; Snail Family Transcription Factors/genetics ; Snail Family Transcription Factors/metabolism ; Epithelial-Mesenchymal Transition/genetics ; Immunohistochemistry ; Retrospective Studies ; Cadherins/genetics ; Cadherins/metabolism ; Osteosarcoma/diagnosis ; Osteosarcoma/pathology ; Bone Neoplasms/diagnosis ; Bone Neoplasms/pathology
    Chemical Substances Snail Family Transcription Factors ; Cadherins
    Language Spanish
    Publishing date 2023-10-25
    Publishing country Chile
    Document type English Abstract ; Journal Article
    ISSN 2452-6053
    ISSN (online) 2452-6053
    DOI 10.32641/andespediatr.v93i6.4255
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Pharmacogenetics of ABCB1, CDA, DCK, GSTT1, GSTM1 and outcomes in a cohort of pediatric acute myeloid leukemia patients from Colombia.

    Yunis, Luz K / Linares-Ballesteros, Adriana / Aponte, Nelson / Barros, Gisela / García, Johnny / Niño, Laura / Uribe, Gloria / Quintero, Edna / Yunis, Juan J

    Cancer reports (Hoboken, N.J.)

    2022  Volume 6, Issue 3, Page(s) e1744

    Abstract: Background and aim: Different studies have shown pharmacogenetic variants related to drug toxicity in acute myeloid leukemia (AML) patients. Our aim was to identify the association between ABCB1, CDA, DCK, GSTT1, and GSTM1 variants with clinical ... ...

    Abstract Background and aim: Different studies have shown pharmacogenetic variants related to drug toxicity in acute myeloid leukemia (AML) patients. Our aim was to identify the association between ABCB1, CDA, DCK, GSTT1, and GSTM1 variants with clinical outcomes and toxicity in pediatric patients with AML.
    Methods: Fifty-one confirmed de novo AML pediatric patients were included. A SNaPshot™ assay and conventional PCR were used to evaluate ABCB1, CDA, DCK, GSTT1, and GSTM1 variants. Clinical outcomes and toxicity associations were evaluated using odds ratios and Chi-square analysis.
    Results: Patients carrying ABCB1 (1236C > T, rs1128503) GG genotype in had a 6.8 OR (CI 95% 1.08-42.73, p = .044) for cardiotoxicity as compared to patients carrying either AA or GA genotypes 0.14 OR (CI 95% 0.023-0.92, p = .044). For ABCB1 (1236G > A rs1128503/2677C > A/T rs2032582/3435G > A rs1045642) AA/AA/AA combined genotypes had a strong association with death after HSTC OR 13.73 (CI 95% 1.94-97.17, p = .009). Combined genotypes GG/CC/GG with CDA (79A > C, rs2072671) CA genotype or CDA (-451G > A, rs532545) CT genotype, had a 4.11 OR (CI 95% 2.32-725, p = .007) and 3.8 OR (CI 95% 2.23-6.47, p = .027) with MRD >0.1% after first chemotherapy cycle, respectively.
    Conclusion: Our results highlight the importance of pharmacogenetic analysis in pediatric AML, particularly in populations with a high degree of admixture, and might be useful as a future tool for patient stratification for treatment.
    MeSH term(s) Humans ; Child ; Pharmacogenetics ; Colombia/epidemiology ; Leukemia, Myeloid, Acute/drug therapy ; Leukemia, Myeloid, Acute/genetics ; Genotype ; ATP Binding Cassette Transporter, Subfamily B/genetics ; ATP Binding Cassette Transporter, Subfamily B/therapeutic use
    Chemical Substances ABCB1 protein, human ; ATP Binding Cassette Transporter, Subfamily B
    Language English
    Publishing date 2022-10-31
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2573-8348
    ISSN (online) 2573-8348
    DOI 10.1002/cnr2.1744
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Genomic alterations in a cohort of pediatric acute myeloid leukemia patients at two cancer centers in Colombia.

    Yunis, Luz K / Linares-Ballesteros, Adriana / Barros, Gisela / Garcia, Johnny / Aponte, Nelson / Niño, Laura / Uribe, Gloria / Quintero, Edna / Perez, Jaime / Martinez, Leila / Yunis, Juan J

    International journal of hematology

    2022  Volume 117, Issue 2, Page(s) 269–277

    Abstract: Few studies identifying genomic aspects in pediatric acute myeloid leukemia patients in Latin American countries have been reported. The aim of this study was to identify genomic alterations, clinical characteristics and outcomes in a cohort of pediatric ...

    Abstract Few studies identifying genomic aspects in pediatric acute myeloid leukemia patients in Latin American countries have been reported. The aim of this study was to identify genomic alterations, clinical characteristics and outcomes in a cohort of pediatric AML patients. This descriptive observational cohort study included patients with confirmed de novo acute myeloid leukemia up to 18 years of age. Cytogenetics and conventional FISH analysis, next-generation sequencing and PCR testing were performed. The correlation of genomic data with treatment response and outcomes were analyzed. Of the 51 patients analyzed, 67.4% had a cytogenetic abnormality and 74.5% had a genetic variant. FLT3 variants (ITD or TKD D835) were found in 27.4%, followed by NRAS (21.6%), KRAS (13.7%) and WT1 and KIT (11.8%). Patients were stratified by risk (66.6% high-risk) after the end of induction. FLT3-ITD was associated with relapse (OR 11.25; CI 1.89-66.72, p 0.006) and NRAS with death during induction (OR 16.71; CI 1.51-184.59, p 0.022). Our study highlights the importance of rapid incorporation of genetic testing in pediatric AML in Colombia, as it directly affects treatment decisions and outcomes. Incorporation of targeted therapies with conventional chemotherapy is an increasingly urgent need in pediatric patients.
    MeSH term(s) Humans ; Colombia/epidemiology ; Leukemia, Myeloid, Acute/genetics ; Leukemia, Myeloid, Acute/drug therapy ; Chromosome Aberrations ; Recurrence ; Genomics ; Mutation ; Prognosis ; fms-Like Tyrosine Kinase 3/genetics
    Chemical Substances fms-Like Tyrosine Kinase 3 (EC 2.7.10.1)
    Language English
    Publishing date 2022-10-24
    Publishing country Japan
    Document type Observational Study ; Journal Article
    ZDB-ID 1076875-0
    ISSN 1865-3774 ; 0917-1258 ; 0925-5710
    ISSN (online) 1865-3774
    ISSN 0917-1258 ; 0925-5710
    DOI 10.1007/s12185-022-03475-w
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 269: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Clinical Characteristics and Outcomes of a Cohort of Pediatric Oncohematologic Patients With COVID-19 Infection in the City of Bogotá, Colombia.

    Fonseca, Eileen V / Pardo, Carlos A / Linares, Adriana / López, Juan F / Camacho, German / Aponte, Nelson H / Bravo, Diana L / Orozco, Daniela / Estupiñan, Marcela / Chaparro, Mauricio

    The Pediatric infectious disease journal

    2021  Volume 40, Issue 6, Page(s) 499–502

    Abstract: Background: In children, the complications of severe acute respiratory syndrome coronavirus 2 infection occur less frequently than in adults but the characteristics of this disease in oncology patients are not well characterized.: Methods: This was a ...

    Abstract Background: In children, the complications of severe acute respiratory syndrome coronavirus 2 infection occur less frequently than in adults but the characteristics of this disease in oncology patients are not well characterized.
    Methods: This was a retrospective study in patients younger than 18 years of age with coronavirus disease 2019 (COVID-19) and cancer diagnoses between April and September 2020. Demographic variables, laboratory, and radiologic findings and complications of each case were identified. A descriptive analysis was performed.
    Results: A total of 33 patients were identified; the median age was 10 years. Fifteen patients (42%) were in chemotherapy at the time of the infection diagnosis, in two patients the chemotherapy protocol was permanently suspended. The most common symptom was fever in 20 patients (60%). Seven patients (21.2%) showed mild pneumonia, four patients (12.1%) severe pneumonia, and three cases (9.0%) were classified as critical. In the evaluated cohort, five patients (15.1%) died, and in two of those, death was caused by COVID-19 infection.
    Conclusions: Children with an oncologic disease, the search for COVID cases should be oriented to patients with fever, including febrile neutropenia, the presence of respiratory symptoms, and the search for epidemiologic contact. A higher frequency of complications and mortality attributed to COVID-19, two in pediatric oncohematologic patients was found. Institutional strategies to detect the infection early and lower institutional infection are indicated.
    MeSH term(s) Adolescent ; COVID-19/mortality ; COVID-19/physiopathology ; COVID-19/therapy ; Child ; Child, Preschool ; Cohort Studies ; Hematologic Neoplasms/mortality ; Hematologic Neoplasms/physiopathology ; Hematologic Neoplasms/virology ; Humans ; Infant ; Pandemics ; Retrospective Studies ; SARS-CoV-2/isolation & purification ; Treatment Outcome
    Language English
    Publishing date 2021-05-10
    Publishing country United States
    Document type Journal Article
    ZDB-ID 392481-6
    ISSN 1532-0987 ; 0891-3668
    ISSN (online) 1532-0987
    ISSN 0891-3668
    DOI 10.1097/INF.0000000000003135
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1852: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Philadelphia-like acute lymphoblastic leukemia: Characterization in a pediatric cohort in a referral center in Colombia.

    Linares Ballesteros, Adriana / Yunis, Luz Karime / García, Johnny / Aponte, Nelson / Flechas, Jessica / Martinez, Cindy / Uribe, Gloria / Quintero, Edna / Díaz, Angela / Pardo, Carlos / Sarmiento, Isabel Cristina / Contreras, Agustin / Yunis, Juan Jose

    Cancer reports (Hoboken, N.J.)

    2021  Volume 5, Issue 5, Page(s) e1587

    Abstract: Background: Philadelphia-like (Ph-like) acute lymphoblastic leukemia (ALL) is a subtype of pediatric leukemia with high risk factors and poor outcome. There are few reports of its prevalence in Latin America.: Aim: This study evaluated the frequency ... ...

    Abstract Background: Philadelphia-like (Ph-like) acute lymphoblastic leukemia (ALL) is a subtype of pediatric leukemia with high risk factors and poor outcome. There are few reports of its prevalence in Latin America.
    Aim: This study evaluated the frequency and clinical and biological characteristics of Ph-like ALL in a pediatric cancer center in Colombia.
    Methods: The Ph-like genetic profile was analyzed by a low-density array (LDA). Samples from patients with Ph-like ALL were analyzed by fluorescent in situ hybridization for cytokine receptor like factor 2 (CRLF2) and ABL proto-oncogene 1, non-receptor tyrosine kinase (ABL1) rearrangements. Copy number variations were assessed by multiplex ligation probe amplification.
    Results: Data from 121 patients were analyzed. Fifteen patients (12.4%) had Ph-like ALL, and these patients had significantly higher leukocyte counts at diagnosis and higher levels of minimal residual disease on days 15 and 33 of induction than patients without the Ph-like subtype. There were no significant differences in sex, age, or response to prednisone at day 8 between the two groups. CRLF2 rearrangements were identified in eight patients, and ABL1 rearrangements were identified in two patients. Other genetic alterations alone or in combination were identified in 77% of patients, including deletions in cyclin dependent kinase inhibitor 2 A/B (46.2%), IKAROS family zinc finger 1 (38.3%), and paired box 5 (30.8%).
    Conclusions: Ph-like ALL had a 12.4% prevalence in our cohort of patients with pediatric ALL. The identification of this group of patients has importance for risk stratification and future targeted therapy.
    MeSH term(s) Child ; Colombia/epidemiology ; DNA Copy Number Variations ; Humans ; Ikaros Transcription Factor/genetics ; In Situ Hybridization, Fluorescence ; Precursor Cell Lymphoblastic Leukemia-Lymphoma/diagnosis ; Precursor Cell Lymphoblastic Leukemia-Lymphoma/drug therapy ; Precursor Cell Lymphoblastic Leukemia-Lymphoma/epidemiology ; Referral and Consultation
    Chemical Substances Ikaros Transcription Factor (148971-36-2)
    Language English
    Publishing date 2021-11-17
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2573-8348
    ISSN (online) 2573-8348
    DOI 10.1002/cnr2.1587
    Database MEDical Literature Analysis and Retrieval System OnLINE

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