Article: Characterisation of an acid trehalase produced by the thermotolerant fungus Rhizopus microsporus var. rhizopodiformis: biochemical properties and immunochemical localisation.
2005 Volume 251, Issue 1, Page(s) 169–175
Abstract: An acid trehalase from Rhizopus microsporus var. rhizopodiformis was purified to apparent homogeneity. The molecular weight by SDS-PAGE (60 kDa) or Sephacryl S-200 filtration (105 kDa) suggested a homodimer. The carbohydrate content was 72%. ... ...
Abstract | An acid trehalase from Rhizopus microsporus var. rhizopodiformis was purified to apparent homogeneity. The molecular weight by SDS-PAGE (60 kDa) or Sephacryl S-200 filtration (105 kDa) suggested a homodimer. The carbohydrate content was 72%. Endoglycosidase H digestion resulted in one sharp band of 51.5 kDa in SDS-PAGE. pH and temperature optima were 4.5 and 45 degrees C, respectively. The isoelectric point was 6.69 and activation energy was 1.14 kcal mol(-1). The enzyme was stable for 1 h at 50 degrees C and decayed at 60 degrees C (t50 of 1.3 min.). Apparent KM for trealose was 0.2mM. Immunolocalisation studies showed the enzyme tightly packed at the surface of the cells. |
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MeSH term(s) | Carbohydrates/analysis ; Dimerization ; Edetic Acid/pharmacology ; Enzyme Activators/pharmacology ; Enzyme Stability ; Hydrogen-Ion Concentration ; Isoelectric Point ; Kinetics ; Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase/metabolism ; Metals/pharmacology ; Molecular Weight ; Rhizopus/chemistry ; Rhizopus/enzymology ; Temperature ; Trehalase/chemistry ; Trehalase/isolation & purification ; Trehalase/metabolism ; Trehalose/metabolism |
Chemical Substances | Carbohydrates ; Enzyme Activators ; Metals ; Edetic Acid (9G34HU7RV0) ; Trehalose (B8WCK70T7I) ; Trehalase (EC 3.2.1.28) ; Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase (EC 3.2.1.96) |
Language | English |
Publishing date | 2005-10-01 |
Publishing country | England |
Document type | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 752343-9 |
ISSN | 1574-6968 ; 0378-1097 |
ISSN (online) | 1574-6968 |
ISSN | 0378-1097 |
DOI | 10.1016/j.femsle.2005.07.045 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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