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Article ; Online: Genome Sequences of 17 Strains from Eight Races of Xanthomonas campestris pv. campestris.

Bellenot, Caroline / Carrère, Sébastien / Gris, Carine / Noël, Laurent D / Arlat, Matthieu

Microbiology resource announcements

2022 Volume 11, Issue 7, Page(s) e0027922

Abstract: Xanthomonas campestris pv. campestris is a group of phytopathogenic bacteria causing black rot disease on Brassicaceae crops. Here, we report on draft genome sequences of 17 strains representing eight of nine known races of this pathogen, including the ... ...

Abstract	Xanthomonas campestris pv. campestris is a group of phytopathogenic bacteria causing black rot disease on Brassicaceae crops. Here, we report on draft genome sequences of 17 strains representing eight of nine known races of this pathogen, including the pathotype strain CFBP 6865.
Language	English
Publishing date	2022-06-13
Publishing country	United States
Document type	Journal Article
ISSN	2576-098X
ISSN (online)	2576-098X
DOI	10.1128/mra.00279-22
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Genome‐wide identification of fitness determinants in the Xanthomonas campestris bacterial pathogen during early stages of plant infection

Luneau, Julien S. / Baudin, Maël / Quiroz Monnens, Thomas / Carrère, Sébastien / Bouchez, Olivier / Jardinaud, Marie‐Françoise / Gris, Carine / François, Jonas / Ray, Jayashree / Torralba, Babil / Arlat, Matthieu / Lewis, Jennifer D. / Lauber, Emmanuelle / Deutschbauer, Adam M. / Noël, Laurent D. / Boulanger, Alice

The new phytologist. 2022 Oct., v. 236, no. 1

2022

Abstract: Plant diseases are an important threat to food production. While major pathogenicity determinants required for disease have been extensively studied, less is known on how pathogens thrive during host colonization, especially at early infection stages. ... ...

Abstract	Plant diseases are an important threat to food production. While major pathogenicity determinants required for disease have been extensively studied, less is known on how pathogens thrive during host colonization, especially at early infection stages. Here, we used randomly barcoded‐transposon insertion site sequencing (RB‐TnSeq) to perform a genome‐wide screen and identify key bacterial fitness determinants of the vascular pathogen Xanthomonas campestris pv campestris (Xcc) during infection of the cauliflower host plant (Brassica oleracea). This high‐throughput analysis was conducted in hydathodes, the natural entry site of Xcc, in xylem sap and in synthetic media. Xcc did not face a strong bottleneck during hydathode infection. In total, 181 genes important for fitness were identified in plant‐associated environments with functional enrichment in genes involved in metabolism but only few genes previously known to be involved in virulence. The biological relevance of 12 genes was independently confirmed by phenotyping single mutants. Notably, we show that XC_3388, a protein with no known function (DUF1631), plays a key role in the adaptation and virulence of Xcc possibly through c‐di‐GMP‐mediated regulation. This study revealed yet unsuspected social behaviors adopted by Xcc individuals when confined inside hydathodes at early infection stages.
Keywords	Brassica oleracea ; Xanthomonas campestris pv. campestris ; cauliflower ; food production ; host plants ; metabolism ; pathogens ; phenotype ; sap ; virulence ; xylem
Language	English
Dates of publication	2022-10
Size	p. 235-248.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	208885-x
ISSN	1469-8137 ; 0028-646X
ISSN (online)	1469-8137
ISSN	0028-646X
DOI	10.1111/nph.18313
Database	NAL-Catalogue (AGRICOLA)

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Article: Xanthomonas transcriptome inside cauliflower hydathodes reveals bacterial virulence strategies and physiological adaptations at early infection stages

Luneau, Julien S. / Cerutti, Aude / Roux, Brice / Carrère, Sébastien / Jardinaud, Marie‐Françoise / Gaillac, Antoine / Gris, Carine / Lauber, Emmanuelle / Berthomé, Richard / Arlat, Matthieu / Boulanger, Alice / Noël, Laurent D.

Molecular plant pathology. 2022 Feb., v. 23, no. 2

2022

Abstract: Xanthomonas campestris pv. campestris (Xcc) is a seed‐transmitted vascular pathogen causing black rot disease on cultivated and wild Brassicaceae. Xcc enters the plant tissues preferentially via hydathodes, which are organs localized at leaf margins. To ... ...

Abstract	Xanthomonas campestris pv. campestris (Xcc) is a seed‐transmitted vascular pathogen causing black rot disease on cultivated and wild Brassicaceae. Xcc enters the plant tissues preferentially via hydathodes, which are organs localized at leaf margins. To decipher both physiological and virulence strategies deployed by Xcc during early stages of infection, the transcriptomic profile of Xcc was analysed 3 days after entry into cauliflower hydathodes. Despite the absence of visible plant tissue alterations and despite a biotrophic lifestyle, 18% of Xcc genes were differentially expressed, including a striking repression of chemotaxis and motility functions. The Xcc full repertoire of virulence factors had not yet been activated but the expression of the HrpG regulon composed of 95 genes, including genes coding for the type III secretion machinery important for suppression of plant immunity, was induced. The expression of genes involved in metabolic adaptations such as catabolism of plant compounds, transport functions, sulphur and phosphate metabolism was upregulated while limited stress responses were observed 3 days postinfection. We confirmed experimentally that high‐affinity phosphate transport is needed for bacterial fitness inside hydathodes. This analysis provides information about the nutritional and stress status of bacteria during the early biotrophic infection stages and helps to decipher the adaptive strategy of Xcc to the hydathode environment.
Keywords	Brassicaceae ; Xanthomonas campestris pv. campestris ; catabolism ; cauliflower ; chemotaxis ; immunity ; leaves ; lifestyle ; pathogens ; phosphates ; plant pathology ; plant tissues ; regulon ; secretion ; sulfur ; transcriptome ; transcriptomics ; virulence
Language	English
Dates of publication	2022-02
Size	p. 159-174.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	2020755-4
ISSN	1364-3703 ; 1464-6722
ISSN (online)	1364-3703
ISSN	1464-6722
DOI	10.1111/mpp.13117
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Genome-wide identification of fitness determinants in the Xanthomonas campestris bacterial pathogen during early stages of plant infection.

Luneau, Julien S / Baudin, Maël / Quiroz Monnens, Thomas / Carrère, Sébastien / Bouchez, Olivier / Jardinaud, Marie-Françoise / Gris, Carine / François, Jonas / Ray, Jayashree / Torralba, Babil / Arlat, Matthieu / Lewis, Jennifer D / Lauber, Emmanuelle / Deutschbauer, Adam M / Noël, Laurent D / Boulanger, Alice

The New phytologist

2022 Volume 236, Issue 1, Page(s) 235–248

Abstract	Plant diseases are an important threat to food production. While major pathogenicity determinants required for disease have been extensively studied, less is known on how pathogens thrive during host colonization, especially at early infection stages. Here, we used randomly barcoded-transposon insertion site sequencing (RB-TnSeq) to perform a genome-wide screen and identify key bacterial fitness determinants of the vascular pathogen Xanthomonas campestris pv campestris (Xcc) during infection of the cauliflower host plant (Brassica oleracea). This high-throughput analysis was conducted in hydathodes, the natural entry site of Xcc, in xylem sap and in synthetic media. Xcc did not face a strong bottleneck during hydathode infection. In total, 181 genes important for fitness were identified in plant-associated environments with functional enrichment in genes involved in metabolism but only few genes previously known to be involved in virulence. The biological relevance of 12 genes was independently confirmed by phenotyping single mutants. Notably, we show that XC_3388, a protein with no known function (DUF1631), plays a key role in the adaptation and virulence of Xcc possibly through c-di-GMP-mediated regulation. This study revealed yet unsuspected social behaviors adopted by Xcc individuals when confined inside hydathodes at early infection stages.
MeSH term(s)	Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Brassica/microbiology ; Plant Diseases/microbiology ; Virulence/genetics ; Xanthomonas campestris ; Xylem/metabolism
Chemical Substances	Bacterial Proteins
Language	English
Publishing date	2022-07-02
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	208885-x
ISSN	1469-8137 ; 0028-646X
ISSN (online)	1469-8137
ISSN	0028-646X
DOI	10.1111/nph.18313
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Bonn / Germany

Z 868: Show issues

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Article ; Online: Xanthomonas transcriptome inside cauliflower hydathodes reveals bacterial virulence strategies and physiological adaptations at early infection stages.

Luneau, Julien S / Cerutti, Aude / Roux, Brice / Carrère, Sébastien / Jardinaud, Marie-Françoise / Gaillac, Antoine / Gris, Carine / Lauber, Emmanuelle / Berthomé, Richard / Arlat, Matthieu / Boulanger, Alice / Noël, Laurent D

Molecular plant pathology

2021 Volume 23, Issue 2, Page(s) 159–174

Abstract: Xanthomonas campestris pv. campestris (Xcc) is a seed-transmitted vascular pathogen causing black rot disease on cultivated and wild Brassicaceae. Xcc enters the plant tissues preferentially via hydathodes, which are organs localized at leaf margins. To ... ...

Abstract	Xanthomonas campestris pv. campestris (Xcc) is a seed-transmitted vascular pathogen causing black rot disease on cultivated and wild Brassicaceae. Xcc enters the plant tissues preferentially via hydathodes, which are organs localized at leaf margins. To decipher both physiological and virulence strategies deployed by Xcc during early stages of infection, the transcriptomic profile of Xcc was analysed 3 days after entry into cauliflower hydathodes. Despite the absence of visible plant tissue alterations and despite a biotrophic lifestyle, 18% of Xcc genes were differentially expressed, including a striking repression of chemotaxis and motility functions. The Xcc full repertoire of virulence factors had not yet been activated but the expression of the HrpG regulon composed of 95 genes, including genes coding for the type III secretion machinery important for suppression of plant immunity, was induced. The expression of genes involved in metabolic adaptations such as catabolism of plant compounds, transport functions, sulphur and phosphate metabolism was upregulated while limited stress responses were observed 3 days postinfection. We confirmed experimentally that high-affinity phosphate transport is needed for bacterial fitness inside hydathodes. This analysis provides information about the nutritional and stress status of bacteria during the early biotrophic infection stages and helps to decipher the adaptive strategy of Xcc to the hydathode environment.
MeSH term(s)	Adaptation, Physiological/genetics ; Bacterial Proteins/metabolism ; Brassica/genetics ; Gene Expression Regulation, Bacterial ; Plant Diseases/genetics ; Transcriptome/genetics ; Virulence/genetics ; Xanthomonas/metabolism ; Xanthomonas campestris/genetics
Chemical Substances	Bacterial Proteins
Language	English
Publishing date	2021-11-26
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2020755-4
ISSN	1364-3703 ; 1364-3703
ISSN (online)	1364-3703
ISSN	1364-3703
DOI	10.1111/mpp.13117
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Xylem sap proteomics.

de Bernonville, Thomas Dugé / Albenne, Cécile / Arlat, Matthieu / Hoffmann, Laurent / Lauber, Emmanuelle / Jamet, Elisabeth

Methods in molecular biology (Clifton, N.J.)

2014 Volume 1072, Page(s) 391–405

Abstract: Proteomic analysis of xylem sap has recently become a major field of interest to understand several biological questions related to plant development and responses to environmental clues. The xylem sap appears as a dynamic fluid undergoing changes in its ...

Abstract	Proteomic analysis of xylem sap has recently become a major field of interest to understand several biological questions related to plant development and responses to environmental clues. The xylem sap appears as a dynamic fluid undergoing changes in its proteome upon abiotic and biotic stresses. Unlike cell compartments which are amenable to purification in sufficient amount prior to proteomic analysis, the xylem sap has to be collected in particular conditions to avoid contamination by intracellular proteins and to obtain enough material. A model plant like Arabidopsis thaliana is not suitable for such an analysis because efficient harvesting of xylem sap is difficult. The analysis of the xylem sap proteome also requires specific procedures to concentrate proteins and to focus on proteins predicted to be secreted. Indeed, xylem sap proteins appear to be synthesized and secreted in the root stele or to originate from dying differentiated xylem cells. This chapter describes protocols to collect xylem sap from Brassica species and to prepare total and N-glycoprotein extracts for identification of proteins by mass spectrometry analyses and bioinformatics.
MeSH term(s)	Brassicaceae/metabolism ; Chromatography, Affinity ; Computational Biology ; Electrophoresis, Polyacrylamide Gel ; Mass Spectrometry ; Plant Exudates/metabolism ; Proteome/metabolism ; Proteomics/methods ; Xylem/metabolism
Chemical Substances	Plant Exudates ; Proteome
Language	English
Publishing date	2014
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-62703-631-3_28
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Genome Sequence of Xanthomonas campestris pv. campestris Strain Xca5

Bolot, Stéphanie / Guy, Endrick / Carrere, Sébastien / Barbe, Valérie / Arlat, Matthieu / Noël, Laurent D

Genome announcements. 2013 Feb. 28, v. 1, no. 1

2013

Abstract: An annotated high-quality draft genome sequence for Xanthomonas campestris pv. campestris race 1 strain Xca5 (originally described as X. campestris pv. armoraciae), the causal agent of black rot on Brassicaceae plants, has been determined. This genome ... ...

Abstract	An annotated high-quality draft genome sequence for Xanthomonas campestris pv. campestris race 1 strain Xca5 (originally described as X. campestris pv. armoraciae), the causal agent of black rot on Brassicaceae plants, has been determined. This genome sequence is a valuable resource for comparative genomics within the campestris pathovar.
Keywords	Brassicaceae ; Xanthomonas campestris pv. campestris ; genome ; genomics ; nucleotide sequences ; pathovars
Language	English
Dates of publication	2013-0228
Size	p. e00032-12.
Publishing place	American Society for Microbiology
Document type	Article
ZDB-ID	2704277-7
ISSN	2169-8287
ISSN	2169-8287
DOI	10.1128/genomeA.00032-12
Database	NAL-Catalogue (AGRICOLA)

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Article: Role of the acquisition of a type 3 secretion system in the emergence of novel pathogenic strains of Xanthomonas

Meline, Valérian / Delage, Wesley / Brin, Chrystelle / Li‐Marchetti, Camille / Sochard, Daniel / Arlat, Matthieu / Rousseau, Céline / Darrasse, Armelle / Briand, Martial / Lebreton, Guillaume / Portier, Perrine / Fischer‐Le Saux, Marion / Durand, Karine / Jacques, Marie‐Agnès / Belin, Etienne / Boureau, Tristan

Molecular plant pathology. 2019 Jan., v. 20, no. 1

2019

Abstract: Cases of emergence of novel plant‐pathogenic strains are regularly reported that reduce the yields of crops and trees. However, the molecular mechanisms underlying such emergence are still poorly understood. The acquisition by environmental non‐ ... ...

Abstract	Cases of emergence of novel plant‐pathogenic strains are regularly reported that reduce the yields of crops and trees. However, the molecular mechanisms underlying such emergence are still poorly understood. The acquisition by environmental non‐pathogenic strains of novel virulence genes by horizontal gene transfer has been suggested as a driver for the emergence of novel pathogenic strains. In this study, we tested such an hypothesis by transferring a plasmid encoding the type 3 secretion system (T3SS) and four associated type 3 secreted proteins (T3SPs) to the non‐pathogenic strains of Xanthomonas CFBP 7698 and CFBP 7700, which lack genes encoding T3SS and any previously known T3SPs. The resulting strains were phenotyped on Nicotiana benthamiana using chlorophyll fluorescence imaging and image analysis. Wild‐type, non‐pathogenic strains induced a hypersensitive response (HR)‐like necrosis, whereas strains complemented with T3SS and T3SPs suppressed this response. Such suppression depends on a functional T3SS. Amongst the T3SPs encoded on the plasmid, Hpa2, Hpa1 and, to a lesser extent, XopF1 collectively participate in suppression. Monitoring of the population sizes in planta showed that the sole acquisition of a functional T3SS by non‐pathogenic strains impairs growth inside leaf tissues. These results provide functional evidence that the acquisition via horizontal gene transfer of a T3SS and four T3SPs by environmental non‐pathogenic strains is not sufficient to make strains pathogenic. In the absence of a canonical effector, the sole acquisition of a T3SS seems to be counter‐selective, and further acquisition of type 3 effectors is probably needed to allow the emergence of novel pathogenic strains.
Keywords	Nicotiana benthamiana ; Xanthomonas ; avirulent strains ; chlorophyll ; crop yield ; genes ; horizontal gene transfer ; hypersensitive response ; image analysis ; leaves ; monitoring ; necrosis ; plasmids ; protein secretion ; tissues ; trees ; type III secretion system ; virulence ; virulent strains
Language	English
Dates of publication	2019-01
Size	p. 33-50.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	2020755-4
ISSN	1364-3703 ; 1464-6722
ISSN (online)	1364-3703
ISSN	1464-6722
DOI	10.1111/mpp.12737
Database	NAL-Catalogue (AGRICOLA)

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Article: Genome Sequence of Xanthomonas campestris pv. campestris Strain Xca5.

Bolot, Stéphanie / Guy, Endrick / Carrere, Sébastien / Barbe, Valérie / Arlat, Matthieu / Noël, Laurent D

Genome announcements

2013 Volume 1, Issue 1

Abstract	An annotated high-quality draft genome sequence for Xanthomonas campestris pv. campestris race 1 strain Xca5 (originally described as X. campestris pv. armoraciae), the causal agent of black rot on Brassicaceae plants, has been determined. This genome sequence is a valuable resource for comparative genomics within the campestris pathovar.
Language	English
Publishing date	2013-02-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	2704277-7
ISSN	2169-8287
ISSN	2169-8287
DOI	10.1128/genomeA.00032-12
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: The Plant Pathogen Xanthomonas campestris pv. campestris Exploits N-Acetylglucosamine during Infection

Boulanger, Alice / Zischek, Claudine / Lautier, Martine / Jamet, Stevie / Rival, Pauline / Carrère, Sébastien / Arlat, Matthieu / Lauber, Emmanuelle

mBio. 2014 Oct. 31, v. 5, no. 5

2014

Abstract: ABSTRACT N -Acetylglucosamine (GlcNAc), the main component of chitin and a major constituent of bacterial peptidoglycan, is present only in trace amounts in plants, in contrast to the huge amount of various sugars that compose the polysaccharides of the ... ...

Abstract	ABSTRACT N -Acetylglucosamine (GlcNAc), the main component of chitin and a major constituent of bacterial peptidoglycan, is present only in trace amounts in plants, in contrast to the huge amount of various sugars that compose the polysaccharides of the plant cell wall. Thus, GlcNAc has not previously been considered a substrate exploited by phytopathogenic bacteria during plant infection. Xanthomonas campestris pv. campestris , the causal agent of black rot disease of Brassica plants, expresses a carbohydrate utilization system devoted to GlcNAc exploitation. In addition to genes involved in GlcNAc catabolism, this system codes for four TonB-dependent outer membrane transporters (TBDTs) and eight glycoside hydrolases. Expression of all these genes is under the control of GlcNAc. In vitro experiments showed that X. campestris pv. campestris exploits chitooligosaccharides, and there is indirect evidence that during the early stationary phase, X. campestris pv. campestris recycles bacterium-derived peptidoglycan/muropeptides. Results obtained also suggest that during plant infection and during growth in cabbage xylem sap, X. campestris pv. campestris encounters and metabolizes plant-derived GlcNAc-containing molecules. Specific TBDTs seem to be preferentially involved in the consumption of all these plant-, fungus- and bacterium-derived GlcNAc-containing molecules. This is the first evidence of GlcNAc consumption during infection by a phytopathogenic bacterium. Interestingly, N -glycans from plant N -glycosylated proteins are proposed to be substrates for glycoside hydrolases belonging to the X. campestris pv. campestris GlcNAc exploitation system. This observation extends the range of sources of GlcNAc metabolized by phytopathogenic bacteria during their life cycle.
Keywords	Brassica ; N-acetylglucosamine ; Xanthomonas campestris pv. campestris ; cabbage ; cell walls ; chitin ; chitooligosaccharides ; fungi ; genes ; glycosides ; hydrolases ; in vitro studies ; insects ; metabolism ; peptidoglycans ; plant pathogenic bacteria ; sap ; transporters ; xylem
Language	English
Dates of publication	2014-1031
Size	p. e01527-14.
Publishing place	American Society for Microbiology
Document type	Article
ZDB-ID	2557172-2
ISSN	2150-7511 ; 2161-2129
ISSN (online)	2150-7511
ISSN	2161-2129
DOI	10.1128/mBio.01527-14
Database	NAL-Catalogue (AGRICOLA)

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