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  1. AU="Artemi, Giulia"
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  1. Article ; Online: Smart nano-sized extracellular vesicles for cancer therapy: Potential theranostic applications in gastrointestinal tumors.

    Lucchetti, Donatella / Colella, Filomena / Artemi, Giulia / Haque, Shafiul / Sgambato, Alessandro / Pellicano, Rinaldo / Fagoonee, Sharmila

    Critical reviews in oncology/hematology

    2023  Volume 191, Page(s) 104121

    Abstract: Extracellular vesicles (EVs) have gained tremendous interest in the search for next-generation therapeutics for the treatment of a range of pathologies, including cancer, especially due to their small size, biomolecular cargo, ability to mediate ... ...

    Abstract Extracellular vesicles (EVs) have gained tremendous interest in the search for next-generation therapeutics for the treatment of a range of pathologies, including cancer, especially due to their small size, biomolecular cargo, ability to mediate intercellular communication, high physicochemical stability, low immunogenicity and biocompatibility. The theranostic potential of EVs have been enhanced by adopting several strategies such as genetic or metabolic engineering, parental cell modification or direct functionalization to incorporate therapeutic compounds into these nanoplatforms. The smart nano-sized EVs indeed offer huge opportunities in the field of cancer, and current research is set at overcoming the existing pitfalls. Smart EVs are already being applied in the clinics despite the challenges faced. We provide, herein, an update on the technologies employed for EV functionalization in order to achieve optimal tumor cell targeting and EV tracking in vivo with bio-imaging modalities, as well as the preclinical and clinical studies making use of these modified EVs, in the context of gastrointestinal tumors.
    MeSH term(s) Humans ; Drug Delivery Systems/methods ; Precision Medicine ; Extracellular Vesicles/chemistry ; Extracellular Vesicles/metabolism ; Extracellular Vesicles/pathology ; Gastrointestinal Neoplasms/diagnosis ; Gastrointestinal Neoplasms/therapy ; Gastrointestinal Neoplasms/metabolism ; Cell Communication
    Language English
    Publishing date 2023-09-09
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 605680-5
    ISSN 1879-0461 ; 0737-9587 ; 1040-8428
    ISSN (online) 1879-0461
    ISSN 0737-9587 ; 1040-8428
    DOI 10.1016/j.critrevonc.2023.104121
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1931: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Cockayne syndrome group A protein localizes at centrosomes during mitosis and regulates Cyclin B1 ubiquitination.

    Paccosi, Elena / Artemi, Giulia / Filippi, Silvia / Balzerano, Alessio / Costanzo, Federico / Laghezza-Masci, Valentina / Proietti, Silvia / Proietti-De-Santis, Luca

    European journal of cell biology

    2023  Volume 102, Issue 2, Page(s) 151325

    Abstract: Mutations in CSA and CSB proteins cause Cockayne syndrome, a rare genetic neurodevelopment disorder. Alongside their demonstrated roles in DNA repair and transcription, these two proteins have recently been discovered to regulate cytokinesis, the final ... ...

    Abstract Mutations in CSA and CSB proteins cause Cockayne syndrome, a rare genetic neurodevelopment disorder. Alongside their demonstrated roles in DNA repair and transcription, these two proteins have recently been discovered to regulate cytokinesis, the final stage of the cell division. This last finding allowed, for the first time, to highlight an extranuclear localization of CS proteins, beyond the one already known at mitochondria. In this study, we demonstrated an additional role for CSA protein being recruited at centrosomes in a strictly determined step of mitosis, which ranges from pro-metaphase until metaphase exit. Centrosomal CSA exerts its function in specifically targeting the pool of centrosomal Cyclin B1 for ubiquitination and proteasomal degradation. Interestingly, a lack of CSA recruitment at centrosomes does not affect Cyclin B1 centrosomal localization but, instead, it causes its lasting centrosomal permanence, thus inducing Caspase 3 activation and apoptosis. The discovery of this unveiled before CSA recruitment at centrosomes opens a new and promising scenario for the understanding of some of the complex and different clinical aspects of Cockayne Syndrome.
    MeSH term(s) Humans ; Cyclin B1/genetics ; Cyclin B1/metabolism ; Cockayne Syndrome/genetics ; Cockayne Syndrome/metabolism ; Mitosis ; Centrosome/metabolism ; Ubiquitination
    Chemical Substances Cyclin B1
    Language English
    Publishing date 2023-05-19
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 391967-5
    ISSN 1618-1298 ; 0070-2463 ; 0171-9335
    ISSN (online) 1618-1298
    ISSN 0070-2463 ; 0171-9335
    DOI 10.1016/j.ejcb.2023.151325
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 667: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Exploiting bioactive natural products of marine origin: Evaluation of the meroterpenoid metachromin V as a novel potential therapeutic drug for colorectal cancer.

    Lucchetti, Donatella / Luongo, Francesca / Colella, Filomena / Gurreri, Enrico / Artemi, Giulia / Desiderio, Claudia / Serra, Stefano / Giuliante, Felice / De Maria, Ruggero / Sgambato, Alessandro / Vitali, Alberto / Fiori, Micol Eleonora

    Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

    2023  Volume 162, Page(s) 114679

    Abstract: Colorectal cancer (CRC) is the second most common cause of cancer death, leading to almost 1 million deaths per year. Despite constant progress in surgical and therapeutic protocols, the 5-year survival rate of advanced CRC patients remains extremely ... ...

    Abstract Colorectal cancer (CRC) is the second most common cause of cancer death, leading to almost 1 million deaths per year. Despite constant progress in surgical and therapeutic protocols, the 5-year survival rate of advanced CRC patients remains extremely poor. Colorectal Cancer Stem Cells (CRC-CSCs) are endowed with unique stemness-related properties responsible for resistance, relapse and metastasis. The development of novel therapeutics able to tackle CSCs while avoiding undesired toxicity is a major need for cancer treatment. Natural products are a large reservoir of unexplored compounds with possible anticancer bioactivity, sustainability, and safety. The family of meroterpenoids derived from sponges share interesting bioactive properties. Bioassay-guided fractionation of a meroterpenoids extract led to the isolation of three compounds, all cytotoxic against several cancer cell lines: Metachromins U, V and W. In this study, we evaluated the anticancer potential of the most active one, Metachromins V (MV), on patient-derived CRC-CSCs. MV strongly impairs CSCs-viability regardless their mutational background and the cytotoxic effect is maintained on therapy-resistant metastatic CSCs. MV affects cell cycle progression, inducing a block in G2 phase in all the cell lines tested and more pronouncedly in CRC-CSCs. Moreover, MV triggers an important reorganization of the cytoskeleton and a strong reduction of Rho GTPases expression, impairing CRC-CSCs motility and invasion ability. By Proteomic analysis identified a potential molecular target of MV: CCAR1, that regulates apoptosis under chemotherapy treatments and affect β-catenin pathway. Further studies will be needed to confirm and validate these data in in vivo experimental models.
    MeSH term(s) Humans ; Proteomics ; Cell Line, Tumor ; Neoplasm Recurrence, Local/pathology ; Colorectal Neoplasms/pathology ; Antineoplastic Agents/pharmacology ; Neoplastic Stem Cells/metabolism ; Cell Cycle Proteins/metabolism ; Apoptosis Regulatory Proteins/metabolism
    Chemical Substances metachromin V ; Antineoplastic Agents ; CCAR1 protein, human ; Cell Cycle Proteins ; Apoptosis Regulatory Proteins
    Language English
    Publishing date 2023-04-15
    Publishing country France
    Document type Journal Article
    ZDB-ID 392415-4
    ISSN 1950-6007 ; 0753-3322 ; 0300-0893
    ISSN (online) 1950-6007
    ISSN 0753-3322 ; 0300-0893
    DOI 10.1016/j.biopha.2023.114679
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.198: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Mutational status of plasma exosomal KRAS predicts outcome in patients with metastatic colorectal cancer.

    Lucchetti, Donatella / Zurlo, Ina Valeria / Colella, Filomena / Ricciardi-Tenore, Claudio / Di Salvatore, Mariantonietta / Tortora, Giampaolo / De Maria, Ruggero / Giuliante, Felice / Cassano, Alessandra / Basso, Michele / Crucitti, Antonio / Laurenzana, Ilaria / Artemi, Giulia / Sgambato, Alessandro

    Scientific reports

    2021  Volume 11, Issue 1, Page(s) 22686

    Abstract: Liquid biopsy has become a useful alternative in metastatic colorectal cancer (mCRC) patients when tissue biopsy of metastatic sites is not feasible. In this study we aimed to investigate the clinical utility of circulating exosomes DNA in the management ...

    Abstract Liquid biopsy has become a useful alternative in metastatic colorectal cancer (mCRC) patients when tissue biopsy of metastatic sites is not feasible. In this study we aimed to investigate the clinical utility of circulating exosomes DNA in the management of mCRC patients. Exosomes level and KRAS mutational status in exosomal DNA was assesed in 70 mCRC patients and 29 CRC primary tumor and were analysed at different disease steps evaluating serial blood samples (240 blood samples). There was a significant correlation between the extension of disease and exosomes level and the resection of primary localized tumor was correlated with a decrease of KRAS G12V/ D copies and fractional abundance in metastatic disease. CEA expression and liver metastasis correlated with a higher number of KRAS G12V/D copies/ml and a higher fractional abundance; in the subgroup of mCRC patients eligible for surgery, the size of tumor and the radiological response were related to exosomes level but only the size was related to the number of KRAS WT copies; both KRAS wild-type and mutated levels were identified as a prognostic factor related to OS. Finally, we found that 91% of mutated mCRC patients became wild type after the first line chemotherapy but this status reverted in mutated one at progression in 80% of cases. In a prospective cohort of mCRC patients, we show how longitudinal monitoring using exosome-based liquid biopsy provides clinical information relevant to therapeutic stratification.
    MeSH term(s) Biomarkers, Tumor/blood ; Biomarkers, Tumor/genetics ; Circulating Tumor DNA/genetics ; Circulating Tumor DNA/isolation & purification ; Colonic Neoplasms/blood ; Colonic Neoplasms/diagnosis ; Colonic Neoplasms/genetics ; Colonic Neoplasms/pathology ; Exosomes/metabolism ; Female ; Follow-Up Studies ; Humans ; Kaplan-Meier Estimate ; Liquid Biopsy/methods ; Male ; Mutation ; Prognosis ; Progression-Free Survival ; Prospective Studies ; Proto-Oncogene Proteins p21(ras)/blood ; Proto-Oncogene Proteins p21(ras)/genetics ; Rectal Neoplasms/blood ; Rectal Neoplasms/diagnosis ; Rectal Neoplasms/genetics ; Rectal Neoplasms/pathology ; Rome/epidemiology
    Chemical Substances Biomarkers, Tumor ; Circulating Tumor DNA ; KRAS protein, human ; Proto-Oncogene Proteins p21(ras) (EC 3.6.5.2)
    Language English
    Publishing date 2021-11-22
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-021-01668-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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