Article ; Online: Evaluation of the Expression of CCR5 and CX3CR1 Receptors and Correlation with the Functionality of T Cells in Women infected with ZIKV during Pregnancy.
2021 Volume 13, Issue 2
Abstract: There have been reports of neurological abnormalities associated with the Zika virus (ZIKV), such as congenital Zika syndrome (CZS) in children born to mothers infected during pregnancy. We investigated how the immune response to ZIKV during pregnancy is ...
| Abstract | There have been reports of neurological abnormalities associated with the Zika virus (ZIKV), such as congenital Zika syndrome (CZS) in children born to mothers infected during pregnancy. We investigated how the immune response to ZIKV during pregnancy is primed and conduct a thorough evaluation of the inflammatory and cytotoxic profiles as well as the expression of CCR5 and CX3CR1. We compared the reactivity of T cells to ZIKV peptides in convalescent mothers infected during pregnancy. The child's clinical outcome (i.e., born with or without CZS) was taken to be the variable. The cells were stimulated in vitro with ZIKV peptides and evaluated using the ELISPOT and flow cytometry assays. After in vitro stimulation with ZIKV peptides, we observed a tendency toward a higher Interferon gamma (IFN-γ)-producing T cell responses in mothers who had asymptomatic children and a higher CD107a expression in T cells in mothers who had children with CZS. We found a higher frequency of T cells expressing CD107a+ and co-expressing CX3CR1+CCR5+, which is much clearer in the T cells of mothers who had CZS children. We suggest that this differential profile influenced the clinical outcome of babies. These data need to be further investigated, including the evaluation of other ZIKV peptides and markers and functional assays. |
|---|---|
| MeSH term(s) | Adult ; CX3C Chemokine Receptor 1/metabolism ; Cross-Sectional Studies ; Cytotoxicity, Immunologic ; Female ; Humans ; Infant ; Interferon-gamma/metabolism ; Lysosomal Membrane Proteins/metabolism ; Pregnancy ; Pregnancy Complications, Infectious/immunology ; Pregnancy Outcome ; Receptors, CCR5/metabolism ; T-Lymphocytes/immunology ; T-Lymphocytes/metabolism ; Young Adult ; Zika Virus/immunology ; Zika Virus Infection/immunology |
| Chemical Substances | CCR5 protein, human ; CX3C Chemokine Receptor 1 ; CX3CR1 protein, human ; LAMP1 protein, human ; Lysosomal Membrane Proteins ; Receptors, CCR5 ; Interferon-gamma (82115-62-6) |
| Language | English |
| Publishing date | 2021-01-28 |
| Publishing country | Switzerland |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2516098-9 |
| ISSN | 1999-4915 ; 1999-4915 |
| ISSN (online) | 1999-4915 |
| ISSN | 1999-4915 |
| DOI | 10.3390/v13020191 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.