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  1. Article ; Online: Three-Dimensional Microscopy by Milling with Ultraviolet Excitation.

    Guo, Jiaming / Artur, Camille / Eriksen, Jason L / Mayerich, David

    Scientific reports

    2019  Volume 9, Issue 1, Page(s) 14578

    Abstract: Analysis of three-dimensional biological samples is critical to understanding tissue function and the mechanisms of disease. Many chronic conditions, like neurodegenerative diseases and cancers, correlate with complex tissue changes that are difficult to ...

    Abstract Analysis of three-dimensional biological samples is critical to understanding tissue function and the mechanisms of disease. Many chronic conditions, like neurodegenerative diseases and cancers, correlate with complex tissue changes that are difficult to explore using two-dimensional histology. While three-dimensional techniques such as confocal and light-sheet microscopy are well-established, they are time consuming, require expensive instrumentation, and are limited to small tissue volumes. Three-dimensional microscopy is therefore impractical in clinical settings and often limited to core facilities at major research institutions. There would be a tremendous benefit to providing clinicians and researchers with the ability to routinely image large three-dimensional tissue volumes at cellular resolution. In this paper, we propose an imaging methodology that enables fast and inexpensive three-dimensional imaging that can be readily integrated into current histology pipelines. This method relies on block-face imaging of paraffin-embedded samples using deep-ultraviolet excitation. The imaged surface is then ablated to reveal the next tissue section for imaging. The final image stack is then aligned and reconstructed to provide tissue models that exceed the depth and resolution achievable with modern three-dimensional imaging systems.
    MeSH term(s) Animals ; Brain/diagnostic imaging ; Cerebral Cortex/diagnostic imaging ; Humans ; Image Processing, Computer-Assisted/methods ; Imaging, Three-Dimensional/methods ; Liver/diagnostic imaging ; Lung/diagnostic imaging ; Mice ; Microcirculation ; Microscopy/methods ; Microscopy, Confocal/methods ; Microscopy, Ultraviolet/methods ; Microtomy/methods ; Monte Carlo Method ; Pattern Recognition, Automated ; Ultraviolet Rays
    Language English
    Publishing date 2019-10-10
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-019-50870-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Multiplex protein-specific microscopy with ultraviolet surface excitation.

    Guo, Jiaming / Artur, Camille / Womack, Tasha / Eriksen, Jason L / Mayerich, David

    Biomedical optics express

    2019  Volume 11, Issue 1, Page(s) 99–108

    Abstract: Immunohistochemical techniques, such as immunofluorescence (IF) staining, enable microscopic imaging of local protein expression within tissue samples. Molecular profiling enabled by IF is critical to understanding pathogenesis and is often involved in ... ...

    Abstract Immunohistochemical techniques, such as immunofluorescence (IF) staining, enable microscopic imaging of local protein expression within tissue samples. Molecular profiling enabled by IF is critical to understanding pathogenesis and is often involved in complex diagnoses. A recent innovation, known as
    Language English
    Publishing date 2019-12-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2572216-5
    ISSN 2156-7085
    ISSN 2156-7085
    DOI 10.1364/BOE.11.000099
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: 10×-Enhanced Heterogeneous Nanocatalysis on a Nanoporous Gold Disk Array with High-Density Hot Spots.

    Arnob, Md Masud Parvez / Artur, Camille / Misbah, Ibrahim / Mubeen, Syed / Shih, Wei-Chuan

    ACS applied materials & interfaces

    2019  Volume 11, Issue 14, Page(s) 13499–13506

    Abstract: Certain noble metal nanostructures as heterogeneous photocatalysts have drawn significant attention in the recent past because of their unique optical properties which lead to the excitation of localized surface plasmon resonance (LSPR). The LSPR ... ...

    Abstract Certain noble metal nanostructures as heterogeneous photocatalysts have drawn significant attention in the recent past because of their unique optical properties which lead to the excitation of localized surface plasmon resonance (LSPR). The LSPR concentrates electromagnetic fields to the surfaces and its relaxation processes can convert photon energy to energetic charge carriers or heat, which can be subsequently harvested to enhance surface catalysis. Here, we report the catalytic performance of a novel plasmonic nanostructure, disk-shaped nanoporous gold (NPG) nanoparticles or simply NPG disks, using a well-tested reduction pathway of resazurin to resorufin. We show that the catalytic reaction rate of NPG disks is enhanced by 10-fold upon external light illumination because of the excitation of LSPR. The plasmon-enhanced catalytic reaction follows a linear-to-superlinear transition in the rate dependence on the input light power. In addition, the light input results in a room temperature reaction rate equivalent to that of an ambient temperature of 70 °C. Together, the results support that hot charge carriers play the dominant role in the enhancement.
    Language English
    Publishing date 2019-03-26
    Publishing country United States
    Document type Journal Article
    ISSN 1944-8252
    ISSN (online) 1944-8252
    DOI 10.1021/acsami.8b19914
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Book ; Online: Three-Dimensional Microscopy by Milling with Ultraviolet Excitation

    Guo, Jiaming / Artur, Camille / Eriksen, Jason L. / Mayerich, David

    2019  

    Abstract: Analysis of three-dimensional biological samples is critical to understanding tissue function and the mechanisms of disease. Many chronic conditions, like neurodegenerative diseases and cancers, correlate with complex tissue changes that are difficult to ...

    Abstract Analysis of three-dimensional biological samples is critical to understanding tissue function and the mechanisms of disease. Many chronic conditions, like neurodegenerative diseases and cancers, correlate with complex tissue changes that are difficult to explore using two-dimensional histology. While three-dimensional techniques such as confocal and light-sheet microscopy are well-established, they are time consuming, require expensive instrumentation, and are limited to small tissue volumes. Three-dimensional microscopy is therefore impractical in clinical settings and often limited to core facilities at major research institutions. There would be a tremendous benefit to providing clinicians and researchers with the ability to routinely image large three-dimensional tissue volumes at cellular resolution. In this paper, we propose an imaging methodology that enables fast and inexpensive three-dimensional imaging that can be readily integrated into current histology pipelines. This method relies on block-face imaging of paraffin-embedded samples using deep-ultraviolet excitation. The imaged surface is then ablated to reveal the next tissue section for imaging. The final image stack is then aligned and reconstructed to provide tissue models that exceed the depth and resolution achievable with modern three-dimensional imaging systems.
    Keywords Physics - Biological Physics ; Physics - Optics
    Subject code 610
    Publishing date 2019-05-03
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article: Plasmonic nanoparticle-based expansion microscopy with surface-enhanced Raman and dark-field spectroscopic imaging.

    Artur, Camille G / Womack, Tasha / Zhao, Fusheng / Eriksen, Jason L / Mayerich, David / Shih, Wei-Chuan

    Biomedical optics express

    2018  Volume 9, Issue 2, Page(s) 603–615

    Abstract: Fluorescence-based expansion microscopy (ExM) is a new technique which can yield nanoscale resolution of biological specimen on a conventional fluorescence microscope through physical sample expansion up to 20 times its original dimensions while ... ...

    Abstract Fluorescence-based expansion microscopy (ExM) is a new technique which can yield nanoscale resolution of biological specimen on a conventional fluorescence microscope through physical sample expansion up to 20 times its original dimensions while preserving structural information. It however inherits known issues of fluorescence microscopy such as photostability and multiplexing capabilities, as well as an ExM-specific issue in signal intensity reduction due to a dilution effect after expansion. To address these issues, we propose using antigen-targeting plasmonic nanoparticle labels which can be imaged using surface-enhanced Raman scattering spectroscopy (SERS) and dark-field spectroscopy. We demonstrate that the nanoparticles enable multimodal imaging: bright-field, dark-field and SERS, with excellent photostability, contrast enhancement and brightness.
    Language English
    Publishing date 2018-01-10
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2572216-5
    ISSN 2156-7085
    ISSN 2156-7085
    DOI 10.1364/BOE.9.000603
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Single-molecule SERS detection of C60.

    Artur, Camille G / Miller, Rowan / Meyer, Matthias / Le Ru, Eric C / Etchegoin, Pablo G

    Physical chemistry chemical physics : PCCP

    2012  Volume 14, Issue 9, Page(s) 3219–3225

    Abstract: Single-molecule Surface-Enhanced Raman Scattering (SERS) detection of buckminsterfullerene (C(60)) is achieved by using different isotopologues of the molecule with a distribution around an average isotopic substitution ((12)C → (13)C) of ~30%. The ... ...

    Abstract Single-molecule Surface-Enhanced Raman Scattering (SERS) detection of buckminsterfullerene (C(60)) is achieved by using different isotopologues of the molecule with a distribution around an average isotopic substitution ((12)C → (13)C) of ~30%. The distribution of different isotopologues creates a broad (~20 cm(-1)) average SERS signal within which single-molecule SERS spectra of individual isotopic realizations of the molecule can be distinguished. The SERS enhancement factors for SM-SERS C(60) events are typically in the range of ~10(8), suggesting a limitation imposed by either photobleaching or surface interactions with the (Ag) metallic colloids to reach the highest SERS hot-spots (which can typically have larger maximum enhancements). SM-SERS signals of isotopically substituted C(60) also show broader peaks (FWHM ≈ 4 cm(-1)) than equivalent signals in natural C(60). The latter feature suggests a contribution to the homogeneous broadening coming from isotopic disorder in the molecule; a feature that can only be observed with the ability to detect single-molecule spectra.
    Language English
    Publishing date 2012-03-07
    Publishing country England
    Document type Journal Article
    ZDB-ID 1476244-4
    ISSN 1463-9084 ; 1463-9076
    ISSN (online) 1463-9084
    ISSN 1463-9076
    DOI 10.1039/c2cp23853e
    Database MEDical Literature Analysis and Retrieval System OnLINE

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