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  1. Article: Ultrasensitive electrochemical genosensors for species-specific diagnosis of malaria.

    Ansah, Felix / Krampa, Francis / Donkor, Jacob K / Owusu-Appiah, Caleb / Ashitei, Sarah / Kornu, Victor E / Danku, Reinhard K / Chirawurah, Jersley D / Awandare, Gordon A / Aniweh, Yaw / Kanyong, Prosper

    Electrochimica acta

    2022  Volume 429, Page(s) 140988

    Abstract: The absence of reliable species-specific diagnostic tools for malaria at point-of-care (POC) remains a major setback towards effective disease management. This is partly due to the limited sensitivity and specificity of the current malaria POC diagnostic ...

    Abstract The absence of reliable species-specific diagnostic tools for malaria at point-of-care (POC) remains a major setback towards effective disease management. This is partly due to the limited sensitivity and specificity of the current malaria POC diagnostic kits especially in cases of low-density parasitaemia and mixed species infections. In this study, we describe the first label-free DNA-based genosensors based on electrochemical impedance spectroscopy (EIS) for species-specific detection of
    Language English
    Publishing date 2022-10-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1483548-4
    ISSN 0013-4686
    ISSN 0013-4686
    DOI 10.1016/j.electacta.2022.140988
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: The temporal dynamics of Plasmodium species infection after artemisinin-based combination therapy (ACT) among asymptomatic children in the Hohoe municipality, Ghana.

    Ansah, Felix / Nyame, Kwamina / Laryea, Rukaya / Owusu, Richard / Amon, Denick / Boyetey, Mark-Jefferson Buer / Ayeke, Dzidzor / Razak, Nasibatu / Kornu, Victor E / Ashitei, Sarah / Owusu-Appiah, Caleb / Chirawurah, Jersley D / Abugri, James / Aniweh, Yaw / Opoku, Nicholas / Sutherland, Colin J / Binka, Fred N / Kweku, Margaret / Awandare, Gordon A /
    Dinko, Bismarck

    Malaria journal

    2023  Volume 22, Issue 1, Page(s) 271

    Abstract: Background: The routine surveillance of asymptomatic malaria using nucleic acid-based amplification tests is essential in obtaining reliable data that would inform malaria policy formulation and the implementation of appropriate control measures.: ... ...

    Abstract Background: The routine surveillance of asymptomatic malaria using nucleic acid-based amplification tests is essential in obtaining reliable data that would inform malaria policy formulation and the implementation of appropriate control measures.
    Methods: In this study, the prevalence rate and the dynamics of Plasmodium species among asymptomatic children (n = 1697) under 5 years from 30 communities within the Hohoe municipality in Ghana were determined.
    Results and discussion: The observed prevalence of Plasmodium parasite infection by polymerase chain reaction (PCR) was 33.6% (571/1697), which was significantly higher compared to that obtained by microscopy [26.6% (451/1697)] (P < 0.0001). Based on species-specific analysis by nested PCR, Plasmodium falciparum infection [33.6% (570/1697)] was dominant, with Plasmodium malariae, Plasmodium ovale and Plasmodium vivax infections accounting for 0.1% (1/1697), 0.0% (0/1697), and 0.0% (0/1697), respectively. The prevalence of P. falciparum infection among the 30 communities ranged from 0.0 to 82.5%. Following artesunate-amodiaquine (AS + AQ, 25 mg/kg) treatment of a sub-population of the participants (n = 184), there was a substantial reduction in Plasmodium parasite prevalence by 100% and 79.2% on day 7 based on microscopy and nested PCR analysis, respectively. However, there was an increase in parasite prevalence from day 14 to day 42, with a subsequent decline on day 70 by both microscopy and nested PCR. For parasite clearance rate analysis, we found a significant proportion of the participants harbouring residual Plasmodium parasites or parasite genomic DNA on day 1 [65.0% (13/20)], day 2 [65.0% (13/20)] and day 3 [60.0% (12/20)] after initiating treatment. Of note, gametocyte carriage among participants was low before and after treatment.
    Conclusion: Taken together, the results indicate that a significant number of individuals could harbour residual Plasmodium parasites or parasite genomic DNA after treatment. The study demonstrates the importance of routine surveillance of asymptomatic malaria using sensitive nucleic acid-based amplification techniques.
    MeSH term(s) Child ; Humans ; Ghana/epidemiology ; Malaria/drug therapy ; Malaria/epidemiology ; Artemisinins/therapeutic use ; Malaria, Falciparum/drug therapy ; Malaria, Falciparum/epidemiology ; Plasmodium malariae ; Nucleic Acids
    Chemical Substances artemisinin (9RMU91N5K2) ; Artemisinins ; Nucleic Acids
    Language English
    Publishing date 2023-09-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 2091229-8
    ISSN 1475-2875 ; 1475-2875
    ISSN (online) 1475-2875
    ISSN 1475-2875
    DOI 10.1186/s12936-023-04712-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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