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  1. Article: Resistance of Sugarcane Cultivar R 570 to Puccinia melanocephala Isolatesfrom Different Geographic Locations.

    Asnaghi, C / D'Hont, A / Glaszmann, J C / Rott, P

    Plant disease

    2019  Volume 85, Issue 3, Page(s) 282–286

    Abstract: Two different inoculation techniques were investigated before studying the reaction of the major rust resistance gene of sugarcane cultivar R 570 against isolates of Puccinia melanocephala from different geographic locations. Cultivar R 570 exhibited ... ...

    Abstract Two different inoculation techniques were investigated before studying the reaction of the major rust resistance gene of sugarcane cultivar R 570 against isolates of Puccinia melanocephala from different geographic locations. Cultivar R 570 exhibited severe rust symptoms when in vitro plantlets were inoculated with a rust isolate from Réunion Island, but a good correlation with field resistance was observed when detached leaves were inoculated with the pathogen. This latter technique was then used to inoculate R 570 and a sample of its self progeny with rust isolates from Brazil, Colombia, Florida (three isolates), Guadeloupe, Réunion Island, and Zimbabwe. R 570 was resistant to all isolates of P. melanocephala, and the segregation of resistance in the progeny did not change with the isolates, suggesting that a single gene, or a single chromosomic region, was involved in the resistance against all tested isolates. This major resistance gene has, therefore, potential value to improve resistance to rust in various geographic regions.
    Language English
    Publishing date 2019-03-04
    Publishing country United States
    Document type Journal Article
    ZDB-ID 754182-x
    ISSN 0191-2917
    ISSN 0191-2917
    DOI 10.1094/PDIS.2001.85.3.282
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Resistance of sugarcane cultivar R 570 to Puccinia melanocephala isolates from different geographic locations

    Asnaghi, C / D'Hont, A / Glaszmann, J.C / Rott, P

    Plant disease. Mar 2001. v. 85 (3)

    2001  

    Abstract: Two different inoculation techniques were investigated before studying the reaction of the major rust resistance gene of sugarcane cultivar R 570 against isolates of Puccinia melanocephala from different geographic locations. Cultivar R 570 exhibited ... ...

    Abstract Two different inoculation techniques were investigated before studying the reaction of the major rust resistance gene of sugarcane cultivar R 570 against isolates of Puccinia melanocephala from different geographic locations. Cultivar R 570 exhibited severe rust symptoms when in vitro plantlets were inoculated with a rust isolate from Reunion Island, but a good correlation with field resistance was observed when detached leaves were inoculated with the pathogen. This latter technique was then used to inoculate R 570 and a sample of its self progeny with rust isolates from Brazil, Colombia, Florida (three isolates), Guadeloupe, Reunion Island, and Zimbabwe. R 570 was resistant to all isolates of P. melanocephala, and the segregation of resistance in the progeny did not change with the isolates, suggesting that a single gene, or a single chromosomic region, was involved in the resistance against all tested isolates. This major resistance gene has, therefore, potential value to improve resistance to rust in various geographic regions.
    Keywords Saccharum ; Puccinia melanocephala ; cultivars ; disease resistance ; provenance ; geographical variation ; genetic resistance ; genes ; leaves ; rust diseases
    Language English
    Dates of publication 2001-03
    Size p. 282-286.
    Document type Article
    ZDB-ID 754182-x
    ISSN 0191-2917
    ISSN 0191-2917
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: Application of synteny across Poaceae to determine the map location of a sugarcane rust resistance gene

    Asnaghi, C / Paulet, F / Kaye, C / Grivet, L / Deu, M / Glaszmann, J.C / D'Hont, A

    Theoretical and applied genetics. Oct 2000. v. 101 (5/6)

    2000  

    Keywords Saccharum ; rust diseases ; Puccinia melanocephala ; disease resistance ; chromosome mapping ; quantitative traits ; loci ; linkage groups ; genetic markers ; restriction fragment length polymorphism ; Sorghum bicolor ; Oryza sativa ; Zea mays ; chromosomes ; species differences
    Language English
    Dates of publication 2000-10
    Size p. 962-969.
    Document type Article
    ZDB-ID 2170-2
    ISSN 1432-2242 ; 0040-5752
    ISSN (online) 1432-2242
    ISSN 0040-5752
    Database NAL-Catalogue (AGRICOLA)

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  4. Article: Targeted mapping of a sugarcane rust resistance gene (Bru1) using bulked segregant analysis and AFLP markers

    Asnaghi, C / Roques, D / Ruffel, S / Kaye, C / Hoarau, J.Y / Telismart, H / Girard, J.C / Raboin, L.M / Risterucci, A.M / Grivet, L

    Theoretical and applied genetics. 2004 Feb., v. 108, no. 4

    2004  

    Abstract: The presence of a major resistance gene (Bru1) for brown rust in the sugarcane cultivar R570 (2n about 115) was confirmed by analyzing segregation of rust resistance in a large population of 658 individuals, derived from selfing of clone R570. A subset ... ...

    Abstract The presence of a major resistance gene (Bru1) for brown rust in the sugarcane cultivar R570 (2n about 115) was confirmed by analyzing segregation of rust resistance in a large population of 658 individuals, derived from selfing of clone R570. A subset of this population was analyzed with AFLP and bulked segregant analysis (BSA) to develop a detailed genetic map around the resistance gene. Four hundred and forty three primer pairs were used resulting in the identification of eight AFLP markers surrounding the resistance gene in an interval of 10 cM, with the closest markers located at 1.9 and 2.2 cM on each side of the gene. Efficiency of the AFLP/BSA applied to the complex polyploid genome of sugarcane is discussed, as well as the potential of the newly identified AFLP markers for developing a map-based cloning approach exploiting, synteny conservation with sorghum.
    Keywords Saccharum ; sugarcane ; major genes ; disease resistance ; genetic resistance ; rust diseases ; Puccinia melanocephala ; plant pathogenic fungi ; chromosome mapping ; genetic markers ; amplified fragment length polymorphism ; genetic techniques and protocols
    Language English
    Dates of publication 2004-02
    Size p. 759-764.
    Document type Article
    ZDB-ID 2170-2
    ISSN 1432-2242 ; 0040-5752
    ISSN (online) 1432-2242
    ISSN 0040-5752
    Database NAL-Catalogue (AGRICOLA)

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  5. Article: Targeted mapping of a sugarcane rust resistance gene (Bru1) using bulked segregant analysis and AFLP markers.

    Asnaghi, C / Roques, D / Ruffel, S / Kaye, C / Hoarau, J-Y / Télismart, H / Girard, J C / Raboin, L M / Risterucci, A M / Grivet, L / D'Hont, A

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

    2004  Volume 108, Issue 4, Page(s) 759–764

    Abstract: The presence of a major resistance gene (Bru1) for brown rust in the sugarcane cultivar R570 (2n about 115) was confirmed by analyzing segregation of rust resistance in a large population of 658 individuals, derived from selfing of clone R570. A subset ... ...

    Abstract The presence of a major resistance gene (Bru1) for brown rust in the sugarcane cultivar R570 (2n about 115) was confirmed by analyzing segregation of rust resistance in a large population of 658 individuals, derived from selfing of clone R570. A subset of this population was analyzed with AFLP and bulked segregant analysis (BSA) to develop a detailed genetic map around the resistance gene. Four hundred and forty three primer pairs were used resulting in the identification of eight AFLP markers surrounding the resistance gene in an interval of 10 cM, with the closest markers located at 1.9 and 2.2 cM on each side of the gene. Efficiency of the AFLP/BSA applied to the complex polyploid genome of sugarcane is discussed, as well as the potential of the newly identified AFLP markers for developing a map-based cloning approach exploiting, synteny conservation with sorghum.
    MeSH term(s) Basidiomycota ; Chromosome Mapping ; DNA Primers ; Immunity, Innate/genetics ; Plant Diseases/microbiology ; Polymorphism, Restriction Fragment Length ; Saccharum/genetics ; Saccharum/microbiology
    Chemical Substances DNA Primers
    Language English
    Publishing date 2004-02
    Publishing country Germany
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2170-2
    ISSN 1432-2242 ; 0040-5752
    ISSN (online) 1432-2242
    ISSN 0040-5752
    DOI 10.1007/s00122-003-1487-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Crosstalk and differential response to abiotic and biotic stressors reflected at the transcriptional level of effector genes from secondary metabolism

    Glombitza, S / Dubuis, P.H / Thulke, O / Welzl, G / Bovet, L / Gotz, M / Affenzeller, M / Geist, B / Hehn, A / Asnaghi, C

    Plant molecular biology. 2004 Apr., v. 54, no. 6

    2004  

    Abstract: Plant secondary metabolism significantly contributes to defensive measures against adverse abiotic and biotic cues. To investigate stress-induced, transcriptional alterations of underlying effector gene families, which encode enzymes acting consecutively ...

    Abstract Plant secondary metabolism significantly contributes to defensive measures against adverse abiotic and biotic cues. To investigate stress-induced, transcriptional alterations of underlying effector gene families, which encode enzymes acting consecutively in secondary metabolism and defense reactions, a DNA array (MetArray) harboring gene-specific probes was established. It comprised complete sets of genes encoding 109 secondary product glycosyltransferases and 63 glutathione-utilizing enzymes along with 62 cytochrome P450 monooxygenases and 26 ABC transporters. Their transcriptome was monitored in different organs of unstressed plants and in shoots in response to herbicides, UV-B radiation, endogenous stress hormones, and pathogen infection. A principal component analysis based on the transcription of these effector gene families defined distinct responses and crosstalk. Methyl jasmonate and ethylene treatments were separated from a group combining reactions towards two sulfonylurea herbicides, salicylate and an avirulent strain of Pseudomonas syringae pv. tomato. The responses to the herbicide bromoxynil and UV-B radiation were distinct from both groups. In addition, these analyses pinpointed individual effector genes indicating their role in these stress responses. A small group of genes was diagnostic in differentiating the response to two herbicide classes used. Interestingly, a subset of genes induced by P. syringae was not responsive to the applied stress hormones. Small groups of comprehensively induced effector genes indicate common defense strategies. Furthermore, homologous members within branches of these effector gene families displayed differential expression patterns either in both organs or during stress responses arguing for their non-redundant functions.
    Keywords Arabidopsis thaliana ; multigene family ; enzymes ; glutathione ; protein metabolism ; glycosyltransferases ; cytochrome P-450 ; ABC transporters ; transcription (genetics) ; gene expression ; messenger RNA ; plant stress ; herbicides ; ultraviolet radiation ; bacterial diseases of plants ; Pseudomonas syringae pv. tomato ; plant pathogenic bacteria
    Language English
    Dates of publication 2004-04
    Size p. 817-835.
    Document type Article
    ZDB-ID 778032-1
    ISSN 1573-5028 ; 0167-4412
    ISSN (online) 1573-5028
    ISSN 0167-4412
    Database NAL-Catalogue (AGRICOLA)

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  7. Article: A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth.

    Abdulrazzak, Nawroz / Pollet, Brigitte / Ehlting, Jürgen / Larsen, Kim / Asnaghi, Carole / Ronseau, Sebastien / Proux, Caroline / Erhardt, Mathieu / Seltzer, Virginie / Renou, Jean-Pierre / Ullmann, Pascaline / Pauly, Markus / Lapierre, Catherine / Werck-Reichhart, Danièle

    Plant physiology

    2006  Volume 140, Issue 1, Page(s) 30–48

    Abstract: Cytochromes P450 monooxygenases from the CYP98 family catalyze the meta-hydroxylation step in the phenylpropanoid biosynthetic pathway. The ref8 Arabidopsis (Arabidopsis thaliana) mutant, with a point mutation in the CYP98A3 gene, was previously ... ...

    Abstract Cytochromes P450 monooxygenases from the CYP98 family catalyze the meta-hydroxylation step in the phenylpropanoid biosynthetic pathway. The ref8 Arabidopsis (Arabidopsis thaliana) mutant, with a point mutation in the CYP98A3 gene, was previously described to show developmental defects, changes in lignin composition, and lack of soluble sinapoyl esters. We isolated a T-DNA insertion mutant in CYP98A3 and show that this mutation leads to a more drastic inhibition of plant development and inhibition of cell growth. Similar to the ref8 mutant, the insertion mutant has reduced lignin content, with stem lignin essentially made of p-hydroxyphenyl units and trace amounts of guaiacyl and syringyl units. However, its roots display an ectopic lignification and a substantial proportion of guaiacyl and syringyl units, suggesting the occurrence of an alternative CYP98A3-independent meta-hydroxylation mechanism active mainly in the roots. Relative to the control, mutant plantlets produce very low amounts of sinapoyl esters, but accumulate flavonol glycosides. Reduced cell growth seems correlated with alterations in the abundance of cell wall polysaccharides, in particular decrease in crystalline cellulose, and profound modifications in gene expression and homeostasis reminiscent of a stress response. CYP98A3 thus constitutes a critical bottleneck in the phenylpropanoid pathway and in the synthesis of compounds controlling plant development. CYP98A3 cosuppressed lines show a gradation of developmental defects and changes in lignin content (40% reduction) and structure (prominent frequency of p-hydroxyphenyl units), but content in foliar sinapoyl esters is similar to the control. The purple coloration of their leaves is correlated to the accumulation of sinapoylated anthocyanins.
    MeSH term(s) Arabidopsis/anatomy & histology ; Arabidopsis/genetics ; Arabidopsis/growth & development ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Cell Enlargement ; Cell Wall/chemistry ; Coumaric Acids/metabolism ; Cytochrome P-450 Enzyme System/genetics ; Cytochrome P-450 Enzyme System/metabolism ; DNA, Bacterial ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Plant ; Genetic Complementation Test ; Hydroxylation ; Lignin/genetics ; Lignin/metabolism ; Mixed Function Oxygenases/genetics ; Mixed Function Oxygenases/metabolism ; Models, Biological ; Molecular Sequence Data ; Mutagenesis, Insertional ; Mutation ; Phenylpropionates/chemistry ; Phenylpropionates/metabolism ; Plant Roots/anatomy & histology ; Plant Roots/metabolism ; Plants, Genetically Modified/anatomy & histology ; Plants, Genetically Modified/genetics ; Plants, Genetically Modified/growth & development ; Polysaccharides/analysis ; RNA, Plant/metabolism
    Chemical Substances Arabidopsis Proteins ; Coumaric Acids ; DNA, Bacterial ; Phenylpropionates ; Polysaccharides ; RNA, Plant ; T-DNA ; Lignin (9005-53-2) ; Cytochrome P-450 Enzyme System (9035-51-2) ; Mixed Function Oxygenases (EC 1.-) ; CYP98A3 protein, Arabidopsis (EC 1.14.-)
    Language English
    Publishing date 2006-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 208914-2
    ISSN 1532-2548 ; 0032-0889
    ISSN (online) 1532-2548
    ISSN 0032-0889
    DOI 10.1104/pp.105.069690
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Crosstalk and differential response to abiotic and biotic stressors reflected at the transcriptional level of effector genes from secondary metabolism.

    Glombitza, Sabine / Dubuis, Pierre-Henri / Thulke, Oliver / Welzl, Gerhard / Bovet, Lucien / Götz, Michael / Affenzeller, Matthias / Geist, Birgit / Hehn, Alain / Asnaghi, Carole / Ernst, Dieter / Seidlitz, Harald K / Gundlach, Heidrun / Mayer, Klaus F / Martinoia, Enrico / Werck-Reichhart, Daniele / Mauch, Felix / Schäffner, Anton R

    Plant molecular biology

    2004  Volume 54, Issue 6, Page(s) 817–835

    Abstract: Plant secondary metabolism significantly contributes to defensive measures against adverse abiotic and biotic cues. To investigate stress-induced, transcriptional alterations of underlying effector gene families, which encode enzymes acting consecutively ...

    Abstract Plant secondary metabolism significantly contributes to defensive measures against adverse abiotic and biotic cues. To investigate stress-induced, transcriptional alterations of underlying effector gene families, which encode enzymes acting consecutively in secondary metabolism and defense reactions, a DNA array (MetArray) harboring gene-specific probes was established. It comprised complete sets of genes encoding 109 secondary product glycosyltransferases and 63 glutathione-utilizing enzymes along with 62 cytochrome P450 monooxygenases and 26 ABC transporters. Their transcriptome was monitored in different organs of unstressed plants and in shoots in response to herbicides, UV-B radiation, endogenous stress hormones, and pathogen infection. A principal component analysis based on the transcription of these effector gene families defined distinct responses and crosstalk. Methyl jasmonate and ethylene treatments were separated from a group combining reactions towards two sulfonylurea herbicides, salicylate and an avirulent strain of Pseudomonas syringae pv. tomato . The responses to the herbicide bromoxynil and UV-B radiation were distinct from both groups. In addition, these analyses pinpointed individual effector genes indicating their role in these stress responses. A small group of genes was diagnostic in differentiating the response to two herbicide classes used. Interestingly, a subset of genes induced by P. syringae was not responsive to the applied stress hormones. Small groups of comprehensively induced effector genes indicate common defense strategies. Furthermore, homologous members within branches of these effector gene families displayed differential expression patterns either in both organs or during stress responses arguing for their non-redundant functions.
    MeSH term(s) Acetates/pharmacology ; Arabidopsis/genetics ; Arabidopsis/metabolism ; Arabidopsis/microbiology ; Cluster Analysis ; Cyclopentanes/pharmacology ; Ethylenes/pharmacology ; Gene Expression Profiling ; Gene Expression Regulation, Plant/drug effects ; Herbicides/pharmacology ; Nitriles/pharmacology ; Oligonucleotide Array Sequence Analysis/methods ; Oxylipins ; Plant Growth Regulators/pharmacology ; Pseudomonas syringae/growth & development ; Salicylic Acid/pharmacology ; Sulfonamides/pharmacology ; Sulfonylurea Compounds/pharmacology ; Transcription, Genetic/drug effects ; Transcription, Genetic/genetics ; Triazines/pharmacology ; Urea/analogs & derivatives ; Urea/pharmacology
    Chemical Substances Acetates ; Cyclopentanes ; Ethylenes ; Herbicides ; Nitriles ; Oxylipins ; Plant Growth Regulators ; Sulfonamides ; Sulfonylurea Compounds ; Triazines ; primisulfuron (7UFU80LX99) ; Urea (8W8T17847W) ; methyl jasmonate (900N171A0F) ; ethylene (91GW059KN7) ; bromoxynil (J46EK95K0P) ; prosulfuron (NG7LE47J14) ; Salicylic Acid (O414PZ4LPZ)
    Language English
    Publishing date 2004-04
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 778032-1
    ISSN 1573-5028 ; 0167-4412
    ISSN (online) 1573-5028
    ISSN 0167-4412
    DOI 10.1007/s11103-004-0274-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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