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Article: Diagnostic accuracy of different bronchoscopic specimens in sputum Xpert MBT/RIF- negative pulmonary TB patients.

Badr, Omaima Ibrahim / Elrefaey, Wael Aly / Shabrawishi, Mohammed / Assaggaf, Hamza M / Minshawi, Faisal

2022 Volume 17, Issue 1, Page(s) 872

Abstract: Background: Tuberculosis (TB) control remains a critical public health problem worldwide. Rapid diagnosis and proper treatment are beneficial for the effective control of tuberculosis transmission. Diagnostic challenges arise when a patient has a ... ...

Abstract	Background: Tuberculosis (TB) control remains a critical public health problem worldwide. Rapid diagnosis and proper treatment are beneficial for the effective control of tuberculosis transmission. Diagnostic challenges arise when a patient has a clinical and radiological suspicion of tuberculosis but cannot produce sputum, sputum acid-fast bacilli, or Xpert Methods: A retrospective study recruited 330 suspected pulmonary TB patients with negative sputum of Xpert MBT/RIF who underwent bronchoscopy between March 2018 and December 2021. The diagnostic yields of bronchoalveolar lavage fluid (acid-fast bacilli, Xpert MTB/RIF, and culture) and histopathologic examination (HPE) were calculated and compared to the final diagnosis and culture as a gold standard. Results: Out of 330 suspected pulmonary TB patients, 181 had a final diagnosis of TB, and 149 had non-TB. The sensitivity of BALF (culture, Xpert, acid-fast bacilli) and trans-bronchial lung biopsy (HPE) was 80.7%, 72.9%, 21.1%, and 87.1%, respectively. Multiple nodules were associated with significantly higher BALF Xpert MTB, bronchoalveolar lavage fluid culture, and trans-bronchial lung biopsy (HPE) positivity. Conclusions: Bronchoscopic specimens are essential for accurate and rapid diagnosis of sputum Xpert MBT/RIF negative patients with high clinical and radiological suspicion of tuberculosis.
Language	English
Publishing date	2022-10-28
Publishing country	Italy
Document type	Journal Article
ZDB-ID	2677839-7
ISSN	2049-6958 ; 1828-695X
ISSN (online)	2049-6958
ISSN	1828-695X
DOI	10.4081/mrm.2022.872
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Investigation of Dombrock Blood Group Alleles and Genotypes among Saudi Blood Donors in Southwestern Saudi Arabia

Halawani, Amr J. / Mansor, Abdullah S. / Assaggaf, Hamza M. / Almasmoum, Hibah A. / Abu-Tawil, Hisham I. / Alsharif, Khalaf F. / Dobie, Gasim / Habibullah, Mahmoud M.

Genes. 2022 June 17, v. 13, no. 6

2022

Abstract: The Dombrock (DO) blood group system has two primary antigens, Doᵃ and Doᵇ, which can cause delayed hemolytic transfusion reactions. The paucity of specific monospecific antibodies can hamper the typing based on these antigens. Thus, blood group ... ...

Abstract	The Dombrock (DO) blood group system has two primary antigens, Doᵃ and Doᵇ, which can cause delayed hemolytic transfusion reactions. The paucity of specific monospecific antibodies can hamper the typing based on these antigens. Thus, blood group genotyping (BGG) was investigated as a possible solution. Sequence-specific primers were designed to target a single nucleotide polymorphism (rs11276) on the ART4 gene encoding the DOA and DOB alleles. Blood samples (n = 150) from randomly selected volunteer donors were used. DNA was extracted and resulting PCR products were purified and sequenced. The allelic frequencies of DOA and DOB were (n = 122, 40.67%) and (n = 178, 59.33%), respectively. The distributions of DO genotypes were as follows: DOA/DOA (n = 20), 13.33%; DOB/DOB (n = 48), 32.00%; and DOA/DOB (n = 82), 54.67%. In conclusion, this study reports on the allelic frequencies of DOA and DOB of the DO blood group system in Jazan Province, Kingdom of Saudi Arabia. Furthermore, this study reports on the prevalence of each genotype, of which DOA/DOB was the most abundant. This study contributes significantly to build the current blood donor database in Southwestern Saudi Arabia. Moreover, it may assist in providing safe blood to polytransfused patients and reduce the risk of the red cell alloimmunization.
Keywords	DNA ; blood ; blood donors ; blood groups ; databases ; genotype ; genotyping ; risk reduction ; single nucleotide polymorphism ; Saudi Arabia
Language	English
Dates of publication	2022-0617
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2527218-4
ISSN	2073-4425
ISSN	2073-4425
DOI	10.3390/genes13061079
Database	NAL-Catalogue (AGRICOLA)

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Article: Predictors of Parental Recall of Newborn Hearing Screening Program in Saudi Arabia.

Almatrafi, Mohammed A / Alsahaf, Nouf / Kabli, Abdulrahman / Maksood, Lama / Alharbi, Khawlah / Alsharif, Alhanouf / Mujahed, Revan A / Naser, Abdallah Y / Assaggaf, Hamza M / Mosalli, Rafat / Alshareef, Shahd / Salawati, Emad

Healthcare (Basel, Switzerland)

2023 Volume 11, Issue 9

Abstract: Hearing impairment is a prevalent disabling condition among children; all newborns should undergo a universal newborn hearing screening (UNHS). Unfortunately, many newborns who fail the screening test are lost to follow-up. Our study aims to evaluate ... ...

Abstract	Hearing impairment is a prevalent disabling condition among children; all newborns should undergo a universal newborn hearing screening (UNHS). Unfortunately, many newborns who fail the screening test are lost to follow-up. Our study aims to evaluate parents' perceptions of UNHS and to identify predictors for newborn hearing screening recall in Saudi Arabia. A cross-sectional study involving Saudi parents with 0-to-18-year-old children born in Saudi Arabia was conducted. Descriptive statistics and binary logistic regression were used to describe the participants' characteristics and to identify UNHS recall predictors. A total of 1533 parents were surveyed. Overall, 29.9% of them recalled a hearing screening at birth, while 22.2% reported no hearing screening, and 47.8% were unable to remember. Only (6.9%) participants reported a failed hearing screening, of which 75.9% recalled a follow-up recommendation. Females, parents aged 30-34 years, consanguineous parents, and parents of newborns who were treated with antibiotics were more likely to recall hearing screening compared to others. This study highlights inadequate awareness of UNHS among parents. Our findings support the need to improve the reporting system of UNHS results and implement educational programs to increase parents' recall of hearing test results and ensure early follow-ups for neonates with failed test results.
Language	English
Publishing date	2023-05-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2721009-1
ISSN	2227-9032
ISSN	2227-9032
DOI	10.3390/healthcare11091357
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Article ; Online: Hand Hygiene Knowledge, Perception, and Practices among Domestic Visitors to the Prophet's Mosque in Al Madinah City Amid the COVID-19 Pandemic: A Cross-Sectional Study.

Mahdi, Hashim A / Assaggaf, Hamza M / Alfelali, Mohammad / Ahmed, Omar B / Alsafi, Radi / Shaban, Ramon Z / Booy, Robert / Rashid, Harunor

International journal of environmental research and public health

2021 Volume 18, Issue 2

Abstract: This study aimed to assess hand hygiene knowledge, perception, and practices of visitors to the Prophet's Mosque in Al Madinah City, Saudi Arabia. Using a self-administered electronic questionnaire, a cross-sectional survey was conducted among domestic ... ...

Abstract	This study aimed to assess hand hygiene knowledge, perception, and practices of visitors to the Prophet's Mosque in Al Madinah City, Saudi Arabia. Using a self-administered electronic questionnaire, a cross-sectional survey was conducted among domestic residents, who visited the mosque between 31 July and 3 August 2020. Participants' demographic data, hand hygiene knowledge, perception, and practices were collected. Four hundred participants aged 18-65 (median 36) years completed the survey, of which 215 (53.8%) were female. The visitors' mean knowledge score about hand hygiene was 6.4 (± standard deviation (SD) 1.35) of total 12. Most participants (392, 98%) were aware of the role of hand hygiene in preventing Coronavirus Disease 2019 (COVID-19); nevertheless, 384 (96%) said hand hygiene lowers body immunity and 316 (79%) thought <60% alcohol is sufficient for hand disinfection. Males had a higher knowledge score than females (6.46 (±1.41) vs. 6.14 (±1.27),
MeSH term(s)	Adolescent ; Adult ; Aged ; COVID-19/prevention & control ; Cross-Sectional Studies ; Female ; Hand Disinfection ; Hand Hygiene ; Health Knowledge, Attitudes, Practice ; Humans ; Male ; Middle Aged ; Pandemics ; Saudi Arabia ; Surveys and Questionnaires ; Young Adult
Language	English
Publishing date	2021-01-14
Publishing country	Switzerland
Document type	Journal Article
ISSN	1660-4601
ISSN (online)	1660-4601
DOI	10.3390/ijerph18020673
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Article: Geo-environmental approach to assess heavy metals around auto-body refinishing shops using bio-monitors

Zeb, Jahan / Tahir, Hajira / Othman, Abdullah / Habeebullah, Turki M. / Sayqal, Ali / Assaggaf, Hamza M. / Ahmed, Omar B. / Sultan, Muhammad / Mohiuddin, Shaikh / Masood, Saiyada Shadiah / Mirza, Agha Zeeshan / Hajira, Bibi

Heliyon. 2022 Jan., v. 8, no. 1

2022

Abstract: The vehicular industry is looking for continuous challenges to develop the sustainability of its manufacturing, maintenance processes, and vehicle emissions due to marketability, environmental, economic, and policy concerns. The present study focuses on ... ...

Abstract	The vehicular industry is looking for continuous challenges to develop the sustainability of its manufacturing, maintenance processes, and vehicle emissions due to marketability, environmental, economic, and policy concerns. The present study focuses on the impact of these processes on the environment. In Pakistan, most of the auto-body refinishing processes are carried out in an open atmosphere. The shades of Azadirachta indica (Neem Tree) are generally used for the outdoor practice of scrapping, grinding, and painting in auto-body refinishing shops of Pakistan. Azadirachta indica leaves were selected as bio-indicator. For the present work, 26 affected sites and 10 control sites were selected from Karachi city, which is the financial hub and biggest city of Pakistan. Concentrations of different metals (Fe, Co, Cd, Cr, Cu, Mn, Mo, Ni, Pb, and Zn) were determined by atomic absorption spectrophotometer. A geographic information system (GIS) is used to present the variation in concentrations within Karachi city. The only positive correlation was observed in Pb and Mn (0.750). Principal component analysis (PCA) is applied to identify the anthropogenic effect between auto-body refinishing areas and control areas. Almost all analyzed metals show higher concentration at affected sites but Pb (87.14 mg/kg), Mn (46.47 mg/kg) and Fe (146.95 mg/kg) were leading the values, as compared to their concentration at control sites, Pb (48.83 mg/kg), Mn (15.23 mg/kg) and Fe (43.07 mg/kg). All analyzed metals are frequently present in different color pigments, whereas Pb, Mn, and Fe may also come from other sources, like the anti-knocking agent, vehicular exhaust, and scraping of car surface.
Keywords	Azadirachta indica ; anthropogenic activities ; atomic absorption spectrometry ; color ; geographic information systems ; indicator species ; issues and policy ; principal component analysis ; Pakistan
Language	English
Dates of publication	2022-01
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2022.e08809
Database	NAL-Catalogue (AGRICOLA)

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Article: The Influence of COVID-19 Pandemic on Influenza Immunization in Saudi Arabia: Cross-Sectional Study.

Minshawi, Faisal / Samannodi, Mohammed / Alwafi, Hassan / Assaggaf, Hamza M / Almatrafi, Mohammed A / Salawati, Emad / Alsafi, Radi / Alharbi, Ruba A / Alduais, Raghad F / Alrehaili, Muruj / Tariq, Shahad / Alghamdi, Rahaf / Almatrfi, Shumok

Journal of multidisciplinary healthcare

2022 Volume 15, Page(s) 1841–1849

Abstract: Introduction: Influenza vaccination is the primary control measure for severe complications caused by influenza viruses. Moreover, in the face of the COVID-19 pandemic, Saudi Arabia recommends vaccinating people at risk against influenza to minimise co- ... ...

Abstract	Introduction: Influenza vaccination is the primary control measure for severe complications caused by influenza viruses. Moreover, in the face of the COVID-19 pandemic, Saudi Arabia recommends vaccinating people at risk against influenza to minimise co-infection risk with SARS-CoV2. Therefore, this study aims to assess the Saudi population's knowledge, attitude, and practice toward influenza vaccination during the COVID-19 pandemic. Moreover, we evaluate the impact of the COVID-19 pandemic on seasonal influenza vaccination. Methods: This cross-sectional study was conducted using an online survey in Saudi Arabia between July to August 2021. Participants were invited to complete the questionnaire through a survey link sent to social media platforms. Results: A total of 2410 participants were included in this study. Our data demonstrate a lack of practice, attitude, and knowledge, especially on the influenza virus's symptoms, viral transmission, and vaccine efficacy. Moreover, this study showed that the COVID-19 pandemic significantly impacted seasonal influenza vaccination in the Saudi population by 1.5-times compared to the previous years. Conclusion: COVID-19 pandemic has increased the hesitancy of Saudi participants in influenza vaccination due to the lack of knowledge. As the pandemic of COVID-19 is fading, awareness campaigns are needed to encourage the public about the importance of receiving the influenza vaccine, especially for those at high risk each year.
Language	English
Publishing date	2022-08-30
Publishing country	New Zealand
Document type	Journal Article
ZDB-ID	2453343-9
ISSN	1178-2390
ISSN	1178-2390
DOI	10.2147/JMDH.S379150
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Article ; Online: Effect of the COVID-19 Vaccine on the Menstrual Cycle among Females in Saudi Arabia.

Qashqari, Fadi S I / Dahlawi, Maryam / Assaggaf, Hamza M / Alsafi, Radi / Gari, Abdulrahim / Abudawood, Abdulrahman / Al-Doboke, Amal / Alsulami, Seham / Bukhari, Rahaf / Majeed, Shaza Adnan / Salih, Elaf Abdullah / Alfelali, Mohammad / Makhdoom, Hatim / Jalal, Naif A

Ethiopian journal of health sciences

2022 Volume 32, Issue 6, Page(s) 1083–1092

Abstract: Background: The number of reports of menstrual changes after COVID-19 vaccination in the Saudi population is still unknown. Therefore, this study aimed to assess the effect of the COVID-19 vaccine(Pfizer, AstraZeneca, and Moderna) on the menstrual cycle ...

Abstract	Background: The number of reports of menstrual changes after COVID-19 vaccination in the Saudi population is still unknown. Therefore, this study aimed to assess the effect of the COVID-19 vaccine(Pfizer, AstraZeneca, and Moderna) on the menstrual cycle among females in Saudi Arabia. Methods: This descriptive cross-sectional study was conducted in Saudi Arabia at Umm Al-Qura University (UQU) from August 2021 to February 2022. Data was collected through a previously validated online questionnaire. Results: A total of 2338 participants who received the first dose of the COVID-19 vaccine participated in this study; 1606 (68.7%) of them received the second dose in addition to the first. The mean age of the study participants was 35.4±9.5 years. No significant associations were found between the type of COVID-19 vaccine and the impact on the menstrual cycle, either for the first or second dose (P-values > 0.05). A significant association was found only between the first dose vaccination day and the impact on the menstrual cycle in the second question of "After receiving the COVID-19 vaccine, your next period was" (P-value ≤ 0.05). Significant associations were found between the second dose vaccination day and the impact on the menstrual cycle in the first and second questions of "After receiving the COVID-19 vaccine, your next period was", and "After receiving the first dose, your next period was," respectively (P-values ≤ 0.05). Conclusion: The study found a potential association between the COVID-19 vaccine and menstrual cycle irregularities, which could impact females' quality of life.
MeSH term(s)	Female ; Humans ; Adult ; COVID-19 Vaccines ; Cross-Sectional Studies ; Quality of Life ; COVID-19/epidemiology ; COVID-19/prevention & control ; Menstrual Cycle
Chemical Substances	COVID-19 Vaccines
Language	English
Publishing date	2022-12-02
Publishing country	Ethiopia
Document type	Journal Article
ZDB-ID	2705970-4
ISSN	2413-7170 ; 2413-7170
ISSN (online)	2413-7170
ISSN	2413-7170
DOI	10.4314/ejhs.v32i6.4
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Article: Geo-environmental approach to assess heavy metals around auto-body refinishing shops using bio-monitors.

Zeb, Jahan / Tahir, Hajira / Othman, Abdullah / Habeebullah, Turki M / Sayqal, Ali / Assaggaf, Hamza M / Ahmed, Omar B / Sultan, Muhammad / Mohiuddin, Shaikh / Masood, Saiyada Shadiah / Mirza, Agha Zeeshan / Hajira, Bibi

Heliyon

2022 Volume 8, Issue 1, Page(s) e08809

Abstract	The vehicular industry is looking for continuous challenges to develop the sustainability of its manufacturing, maintenance processes, and vehicle emissions due to marketability, environmental, economic, and policy concerns. The present study focuses on the impact of these processes on the environment. In Pakistan, most of the auto-body refinishing processes are carried out in an open atmosphere. The shades of Azadirachta indica (Neem Tree) are generally used for the outdoor practice of scrapping, grinding, and painting in auto-body refinishing shops of Pakistan. Azadirachta indica leaves were selected as bio-indicator. For the present work, 26 affected sites and 10 control sites were selected from Karachi city, which is the financial hub and biggest city of Pakistan. Concentrations of different metals (Fe, Co, Cd, Cr, Cu, Mn, Mo, Ni, Pb, and Zn) were determined by atomic absorption spectrophotometer. A geographic information system (GIS) is used to present the variation in concentrations within Karachi city. The only positive correlation was observed in Pb and Mn (0.750). Principal component analysis (PCA) is applied to identify the anthropogenic effect between auto-body refinishing areas and control areas. Almost all analyzed metals show higher concentration at affected sites but Pb (87.14 mg/kg), Mn (46.47 mg/kg) and Fe (146.95 mg/kg) were leading the values, as compared to their concentration at control sites, Pb (48.83 mg/kg), Mn (15.23 mg/kg) and Fe (43.07 mg/kg). All analyzed metals are frequently present in different color pigments, whereas Pb, Mn, and Fe may also come from other sources, like the anti-knocking agent, vehicular exhaust, and scraping of car surface.
Language	English
Publishing date	2022-01-21
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2022.e08809
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Haematological, Biochemical, and Inflammatory Biomarkers of COVID-19 Patients Hospitalized in Critical Unit: A Retrospective Study.

Alsafi, Radi T / Minshawi, Faisal / Alshareef, Ahmad / Althobiany, Essa / Alqurashi, Afnan / Zawawi, Ayat / Qasem, Ahmed / Halawani, Amr J / Almatrafi, Mohammed / Alwafi, Hassan / Samannodi, Mohammed / Salawati, Emad / Assaggaf, Hamza M

Cureus

2022 Volume 14, Issue 3, Page(s) e23691

Abstract: Background: The World Health Organization declared coronavirus disease 2019 (COVID-19) responsible for a catastrophic global pandemic. The complexity of COVID-19 is centred on the unpredictable course of the disease, which can rapidly develop from ... ...

Abstract	Background: The World Health Organization declared coronavirus disease 2019 (COVID-19) responsible for a catastrophic global pandemic. The complexity of COVID-19 is centred on the unpredictable course of the disease, which can rapidly develop from patients being asymptomatic to having life-threatening symptoms. The unpredictable disease severity of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has been a major problem facing the healthcare system during the pandemic. Identifying the laboratory biomarkers would help predict SARS-CoV-2 pathogenicity. This study focused on the previous literature regarding three laboratory biomarker profiles: haematological, inflammatory, and biochemical biomarkers. Methods: A retrospective study of COVID-19 patients was conducted between May 2020 and September 2020 to determine the predictors of hospitalization (severity) in COVID-19 patients. Patients were divided into two groups: those admitted to an intensive care unit (ICU, severe) and those admitted to a non-ICU (stable). Patients' data were obtained from their medical records at Al Noor Specialist Hospital and East Arafat Hospital in Saudi Arabia. Results: A total of 487 patients with COVID-19, including 304 males and 183 females, were investigated in this study. A total of 217 patients were admitted to the ICU. Patients admitted to the ICU had a higher prevalence of chronic comorbidities than non-ICU patients. D-dimer, white blood cells (WBC), neutrophils, ferritin, C-reactive protein (CRP), lactate dehydrogenase (LDH), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were more elevated in patients admitted to the ICU compared to non-ICU patients. Conclusion: Chronic comorbidities are a significant predictor for admission to the ICU. Moreover, tests for D-dimer, WBC, neutrophils, lymphocytes, CRP, LDH, and ALT could be used to predict patients' admission to the ICU.
Language	English
Publishing date	2022-03-31
Publishing country	United States
Document type	Journal Article
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.23691
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Article ; Online: Investigation of Dombrock Blood Group Alleles and Genotypes among Saudi Blood Donors in Southwestern Saudi Arabia.

Halawani, Amr J / Mansor, Abdullah S / Assaggaf, Hamza M / Almasmoum, Hibah A / Abu-Tawil, Hisham I / Alsharif, Khalaf F / Dobie, Gasim / Habibullah, Mahmoud M

Genes

2022 Volume 13, Issue 6

Abstract: The Dombrock (DO) blood group system has two primary antigens, ... ...

Abstract	The Dombrock (DO) blood group system has two primary antigens, Do
MeSH term(s)	Alleles ; Blood Donors ; Blood Group Antigens/genetics ; Genotype ; Humans ; Saudi Arabia/epidemiology
Chemical Substances	Blood Group Antigens
Language	English
Publishing date	2022-06-17
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2527218-4
ISSN	2073-4425 ; 2073-4425
ISSN (online)	2073-4425
ISSN	2073-4425
DOI	10.3390/genes13061079
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