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  1. Article ; Online: Aberrant Adenosine Triphosphate Release and Impairment of P2Y2-Mediated Signaling in Sarcoglycanopathies.

    Benzi, Andrea / Baratto, Serena / Astigiano, Cecilia / Sturla, Laura / Panicucci, Chiara / Mamchaoui, Kamel / Raffaghello, Lizzia / Bruzzone, Santina / Gazzerro, Elisabetta / Bruno, Claudio

    Laboratory investigation; a journal of technical methods and pathology

    2023  Volume 103, Issue 3, Page(s) 100037

    Abstract: Sarcoglycanopathies, limb-girdle muscular dystrophies (LGMD) caused by genetic loss-of-function of the membrane proteins sarcoglycans (SGs), are characterized by progressive degeneration of skeletal muscle. In these disorders, muscle necrosis is ... ...

    Abstract Sarcoglycanopathies, limb-girdle muscular dystrophies (LGMD) caused by genetic loss-of-function of the membrane proteins sarcoglycans (SGs), are characterized by progressive degeneration of skeletal muscle. In these disorders, muscle necrosis is associated with immune-mediated damage, whose triggering and perpetuating molecular mechanisms are not fully elucidated yet. Extracellular adenosine triphosphate (eATP) seems to represent a crucial factor, with eATP activating purinergic receptors. Indeed, in vivo blockade of the eATP/P2X7 purinergic pathway ameliorated muscle disease progression. P2X7 inhibition improved the dystrophic process by restraining the activity of P2X7 receptors on immune cells. Whether P2X7 blockade can display a direct action on muscle cells is not known yet. In this study, we investigated eATP effects in primary cultures of myoblasts isolated from patients with LGMDR3 (α-sarcoglycanopathy) and in immortalized cells isolated from a patient with LGMDR5 (γ-sarcoglycanopathy). Our results demonstrated that, owing to a reduced ecto-ATPase activity and/or an enhanced release of ATP, patient cells are exposed to increased juxtamembrane concentrations of eATP and display a higher susceptivity to eATP signals. The purinoceptor P2Y2, which proved to be overexpressed in patient cells, was identified as a pivotal receptor responsible for the enhanced ATP-induced or UTP-induced Ca
    MeSH term(s) Humans ; Adenosine Triphosphate/metabolism ; Muscle, Skeletal/metabolism ; Sarcoglycanopathies/metabolism ; Signal Transduction ; Receptors, Purinergic P2Y2
    Chemical Substances Adenosine Triphosphate (8L70Q75FXE) ; Receptors, Purinergic P2Y2
    Language English
    Publishing date 2023-01-10
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80178-1
    ISSN 1530-0307 ; 0023-6837
    ISSN (online) 1530-0307
    ISSN 0023-6837
    DOI 10.1016/j.labinv.2022.100037
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.164: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Paracrine ADP Ribosyl Cyclase-Mediated Regulation of Biological Processes.

    Astigiano, Cecilia / Benzi, Andrea / Laugieri, Maria Elena / Piacente, Francesco / Sturla, Laura / Guida, Lucrezia / Bruzzone, Santina / De Flora, Antonio

    Cells

    2022  Volume 11, Issue 17

    Abstract: ADP-ribosyl cyclases (ADPRCs) catalyze the synthesis of the ... ...

    Abstract ADP-ribosyl cyclases (ADPRCs) catalyze the synthesis of the Ca
    MeSH term(s) ADP-ribosyl Cyclase/metabolism ; ADP-ribosyl Cyclase 1/metabolism ; Animals ; Antigens, CD/metabolism ; Biological Phenomena ; Connexin 43/metabolism ; Cyclic ADP-Ribose/metabolism ; Mammals/metabolism ; Membrane Glycoproteins/metabolism ; NAD/metabolism
    Chemical Substances Antigens, CD ; Connexin 43 ; Membrane Glycoproteins ; NAD (0U46U6E8UK) ; Cyclic ADP-Ribose (119340-53-3) ; ADP-ribosyl Cyclase (EC 3.2.2.5) ; ADP-ribosyl Cyclase 1 (EC 3.2.2.6)
    Language English
    Publishing date 2022-08-24
    Publishing country Switzerland
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells11172637
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Sirtuin 6 Regulates the Activation of the ATP/Purinergic Axis in Endothelial Cells.

    Astigiano, Cecilia / Piacente, Francesco / Laugieri, Maria Elena / Benzi, Andrea / Di Buduo, Christian A / Miguel, Carolina P / Soncini, Debora / Cea, Michele / Antonelli, Antonella / Magnani, Mauro / Balduini, Alessandra / De Flora, Antonio / Bruzzone, Santina

    International journal of molecular sciences

    2023  Volume 24, Issue 7

    Abstract: Sirtuin 6 (SIRT6) is a member of the mammalian ... ...

    Abstract Sirtuin 6 (SIRT6) is a member of the mammalian NAD
    MeSH term(s) Humans ; Adenosine Triphosphate ; Human Umbilical Vein Endothelial Cells/metabolism ; NAD ; Sirtuins/metabolism ; Tumor Necrosis Factor-alpha/pharmacology
    Chemical Substances Adenosine Triphosphate (8L70Q75FXE) ; NAD (0U46U6E8UK) ; SIRT6 protein, human (EC 3.5.1.-) ; Sirtuins (EC 3.5.1.-) ; Tumor Necrosis Factor-alpha
    Language English
    Publishing date 2023-04-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24076759
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Anoxia Rapidly Induces Changes in Expression of a Large and Diverse Set of Genes in Endothelial Cells.

    Antonelli, Antonella / Scarpa, Emanuele Salvatore / Bruzzone, Santina / Astigiano, Cecilia / Piacente, Francesco / Bruschi, Michela / Fraternale, Alessandra / Di Buduo, Christian A / Balduini, Alessandra / Magnani, Mauro

    International journal of molecular sciences

    2023  Volume 24, Issue 6

    Abstract: Sinusoidal endothelial cells are the predominant vascular surface of the bone marrow and constitute the functional hematopoietic niche where hematopoietic stem and progenitor cells receive cues for self-renewal, survival, and differentiation. In the bone ...

    Abstract Sinusoidal endothelial cells are the predominant vascular surface of the bone marrow and constitute the functional hematopoietic niche where hematopoietic stem and progenitor cells receive cues for self-renewal, survival, and differentiation. In the bone marrow hematopoietic niche, the oxygen tension is usually very low, and this condition affects stem and progenitor cell proliferation and differentiation and other important functions of this region. Here, we have investigated in vitro the response of endothelial cells to a marked decrease in O
    MeSH term(s) Hematopoietic Stem Cells/metabolism ; Endothelial Cells/metabolism ; Cells, Cultured ; Bone Marrow/metabolism ; Interleukins/metabolism ; Sirtuins/genetics ; Sirtuins/metabolism
    Chemical Substances Interleukins ; Sirtuins (EC 3.5.1.-)
    Language English
    Publishing date 2023-03-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24065157
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Novel Thiazole-Based SIRT2 Inhibitors Discovered via Molecular Modelling Studies and Enzymatic Assays.

    Abbotto, Elena / Casini, Beatrice / Piacente, Francesco / Scarano, Naomi / Cerri, Elena / Tonelli, Michele / Astigiano, Cecilia / Millo, Enrico / Sturla, Laura / Bruzzone, Santina / Cichero, Elena

    Pharmaceuticals (Basel, Switzerland)

    2023  Volume 16, Issue 9

    Abstract: Recently, the development of sirtuin small molecule inhibitors (SIRTIs) has been gaining attention for the treatment of different cancer types, but also to contrast neurodegenerative disease, diabetes, and autoimmune syndromes. In the search for SIRT2 ... ...

    Abstract Recently, the development of sirtuin small molecule inhibitors (SIRTIs) has been gaining attention for the treatment of different cancer types, but also to contrast neurodegenerative disease, diabetes, and autoimmune syndromes. In the search for SIRT2 modulators, the availability of several X-crystallographic data regarding SIRT2-ligand complexes has allowed for setting up a structure-based study, which is herein presented. A set of 116 SIRT2 inhibitors featuring different chemical structures has been collected from the literature and used for molecular docking studies involving 4RMG and 5MAT PDB codes. The information found highlights key contacts with the SIRT2 binding pocket such as Van der Waals and π-π stacking with Tyr104, Phe119, Phe234, and Phe235 in order to achieve high inhibitory ability values. Following the preliminary virtual screening studies, a small in-house library of compounds (
    Language English
    Publishing date 2023-09-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2193542-7
    ISSN 1424-8247
    ISSN 1424-8247
    DOI 10.3390/ph16091316
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Mitochondrial rewiring drives metabolic adaptation to NAD(H) shortage in triple negative breast cancer cells.

    Carreira, Agata Sofia Assuncao / Ravera, Silvia / Zucal, Chiara / Thongon, Natthakan / Caffa, Irene / Astigiano, Cecilia / Bertola, Nadia / Buongiorno, Arianna / Roccuzzo, Michela / Bisio, Alessandra / Pardini, Barbara / Nencioni, Alessio / Bruzzone, Santina / Provenzani, Alessandro

    Neoplasia (New York, N.Y.)

    2023  Volume 41, Page(s) 100903

    Abstract: Nicotinamide phosphoribosyltransferase (NAMPT) is a key metabolic enzyme in ... ...

    Abstract Nicotinamide phosphoribosyltransferase (NAMPT) is a key metabolic enzyme in NAD
    MeSH term(s) Humans ; NAD/metabolism ; Triple Negative Breast Neoplasms/drug therapy ; Triple Negative Breast Neoplasms/genetics ; Cytokines/metabolism ; Nicotinamide Phosphoribosyltransferase/genetics ; Nicotinamide Phosphoribosyltransferase/metabolism ; Mitochondria/metabolism ; Cell Line, Tumor ; Phosphotransferases (Alcohol Group Acceptor)
    Chemical Substances NAD (0U46U6E8UK) ; Cytokines ; Nicotinamide Phosphoribosyltransferase (EC 2.4.2.12) ; NMRK1 protein, human (EC 2.7.1.22) ; Phosphotransferases (Alcohol Group Acceptor) (EC 2.7.1.-)
    Language English
    Publishing date 2023-05-04
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1483840-0
    ISSN 1476-5586 ; 1522-8002
    ISSN (online) 1476-5586
    ISSN 1522-8002
    DOI 10.1016/j.neo.2023.100903
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5203: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  7. Article: The TRPM2 ion channel regulates metabolic and thermogenic adaptations in adipose tissue of cold-exposed mice.

    Benzi, Andrea / Heine, Markus / Spinelli, Sonia / Salis, Annalisa / Worthmann, Anna / Diercks, Björn / Astigiano, Cecilia / Pérez Mato, Raúl / Memushaj, Adela / Sturla, Laura / Vellone, Valerio / Damonte, Gianluca / Jaeckstein, Michelle Y / Koch-Nolte, Friedrich / Mittrücker, Hans-Willi / Guse, Andreas H / De Flora, Antonio / Heeren, Joerg / Bruzzone, Santina

    Frontiers in endocrinology

    2024  Volume 14, Page(s) 1251351

    Abstract: Introduction: During thermogenesis, adipose tissue (AT) becomes more active and enhances oxidative metabolism. The promotion of this process in white AT (WAT) is called "browning" and, together with the brown AT (BAT) activation, is considered as a ... ...

    Abstract Introduction: During thermogenesis, adipose tissue (AT) becomes more active and enhances oxidative metabolism. The promotion of this process in white AT (WAT) is called "browning" and, together with the brown AT (BAT) activation, is considered as a promising approach to counteract obesity and metabolic diseases. Transient receptor potential cation channel, subfamily M, member 2 (TRPM2), is an ion channel that allows extracellular Ca
    Methods: Wild type (WT) and
    Results: Trpm2
    Discussion: Our data indicate TRPM2 as a fundamental player in BAT activation and WAT browning. TRPM2 agonists may represent new pharmacological strategies to fight obesity.
    MeSH term(s) Mice ; Animals ; TRPM Cation Channels/genetics ; TRPM Cation Channels/metabolism ; Adipose Tissue, Brown/metabolism ; Adipose Tissue, White/metabolism ; Obesity/genetics ; Obesity/metabolism ; Thermogenesis/genetics
    Chemical Substances TRPM Cation Channels ; TRPM2 protein, mouse
    Language English
    Publishing date 2024-02-06
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2592084-4
    ISSN 1664-2392
    ISSN 1664-2392
    DOI 10.3389/fendo.2023.1251351
    Database MEDical Literature Analysis and Retrieval System OnLINE

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