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  1. Article ; Online: Happy birthday, Klaus-Rüdiger! Heartfelt appreciation on the occasion of the 80th birthday of Professor Klaus-Rüdiger Trott.

    Combs, Stephanie E / Atkinson, Michael J

    Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al

    2020  Volume 196, Issue 8, Page(s) 747–748

    MeSH term(s) Germany ; History, 20th Century ; History, 21st Century ; Humans ; London ; Radiation Oncology/history ; Radiobiology/history
    Language English
    Publishing date 2020-07-15
    Publishing country Germany
    Document type Biography ; Editorial ; Historical Article
    ZDB-ID 84983-2
    ISSN 1439-099X ; 0179-7158 ; 0039-2073
    ISSN (online) 1439-099X
    ISSN 0179-7158 ; 0039-2073
    DOI 10.1007/s00066-020-01668-y
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  2. Article ; Online: Quantitative Proteomic Analysis Using Formalin-Fixed, Paraffin-Embedded (FFPE) Human Cardiac Tissue.

    Azimzadeh, Omid / Atkinson, Michael J / Tapio, Soile

    Methods in molecular biology (Clifton, N.J.)

    2021  Volume 2261, Page(s) 525–533

    Abstract: Clinical tissue archives represent an invaluable source of biological information. Formalin-fixed, paraffin-embedded (FFPE) tissue can be used for retrospective investigation of biomarkers of diseases and prognosis.Recently, the number of studies using ... ...

    Abstract Clinical tissue archives represent an invaluable source of biological information. Formalin-fixed, paraffin-embedded (FFPE) tissue can be used for retrospective investigation of biomarkers of diseases and prognosis.Recently, the number of studies using proteome profiling of samples from clinical archives has markedly increased. However, the application of conventional quantitative proteomics technologies remains a challenge mainly due to the harsh fixation process resulting in protein cross-linking and protein degradation. In the present chapter, we demonstrate a protocol for label-free proteomic analysis of FFPE tissue prepared from human cardiac autopsies. The data presented here highlight the applicability and suitability of FFPE heart tissue for understanding the molecular mechanism of cardiac injury using a proteomics approach.
    MeSH term(s) Autopsy ; Chromatography, Reverse-Phase ; Fixatives/chemistry ; Formaldehyde/chemistry ; Humans ; Myocardium/metabolism ; Paraffin Embedding ; Proteins/analysis ; Proteome ; Proteomics ; Tandem Mass Spectrometry ; Tissue Fixation
    Chemical Substances Fixatives ; Proteins ; Proteome ; Formaldehyde (1HG84L3525)
    Language English
    Publishing date 2021-01-09
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-1186-9_33
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  3. Article ; Online: Combining HDAC and MEK Inhibitors with Radiation against Glioblastoma-Derived Spheres.

    Essien, Eno I / Hofer, Thomas P / Atkinson, Michael J / Anastasov, Nataša

    Cells

    2022  Volume 11, Issue 5

    Abstract: Glioblastoma stem-like cells (GSLCs) in glioblastoma limit effective treatment and promote therapeutic resistance and tumor recurrence. Using a combined radiation and drug-screening platform, we tested the combination of a histone deacetylase inhibitor ( ... ...

    Abstract Glioblastoma stem-like cells (GSLCs) in glioblastoma limit effective treatment and promote therapeutic resistance and tumor recurrence. Using a combined radiation and drug-screening platform, we tested the combination of a histone deacetylase inhibitor (HDACi) and MAPK/ERK kinase inhibitor (MEKi) with radiation to predict the efficacy against GSLCs. To mimic a stem-like phenotype, glioblastoma-derived spheres were used and treated with a combination of HDACi (MS-275) and MEKi (TAK-733 or trametinib) with 4 Gy irradiation. The sphere-forming ability after the combined radiochemotherapy was investigated using a sphere formation assay, while the expression levels of the GSLC markers (CD44, Nestin and SOX2) after treatment were analyzed using Western blotting and flow cytometry. The combined radiochemotherapy treatment inhibited the sphere formation in both glioblastoma-derived spheres, decreased the expression of the GSLC markers in a cell-line dependent manner and increased the dead cell population. Finally, we showed that the combined treatment with radiation was more effective at reducing the GSLC markers compared to the standard treatment of temozolomide and radiation. These results suggest that combining HDAC and MEK inhibition with radiation may offer a new strategy to improve the treatment of glioblastoma.
    MeSH term(s) Cell Line, Tumor ; Glioblastoma/drug therapy ; Glioblastoma/genetics ; Glioblastoma/radiotherapy ; Histone Deacetylase Inhibitors/pharmacology ; Humans ; Mitogen-Activated Protein Kinase Kinases ; Neoplasm Recurrence, Local ; Protein Kinase Inhibitors/pharmacology ; Temozolomide/pharmacology
    Chemical Substances Histone Deacetylase Inhibitors ; Protein Kinase Inhibitors ; Mitogen-Activated Protein Kinase Kinases (EC 2.7.12.2) ; Temozolomide (YF1K15M17Y)
    Language English
    Publishing date 2022-02-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells11050775
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  4. Article: Radiation treatment effects on the proteome of the tumour microenvironment.

    Atkinson, Michael J

    Advances in experimental medicine and biology

    2013  Volume 990, Page(s) 49–60

    Abstract: Exposure of tumourous tissue to ionizing radiation initiates a wound-healing response involving remodelling of the extracellular microenvironment. The initial reaction involves direct damage to the matrix proteins and the secretion and activation of ... ...

    Abstract Exposure of tumourous tissue to ionizing radiation initiates a wound-healing response involving remodelling of the extracellular microenvironment. The initial reaction involves direct damage to the matrix proteins and the secretion and activation of proteolytic enzymes that lead to local destruction of the extracellular matrix. Subsequently the wounded area may undergo complete repair, may enter a prolonged period of heightened proteolysis, or may overproduce matrix proteins leading to fibrosis. The source of matrix degrading enzymatic activity may be the tumour cells and the tumour stroma. Additional complexity is provided by proteolytic activity released from tissue macrophages, mast cells and by invading inflammatory cells. The local production of growth factors, including VEGF and TGF-β play a key role in coordinating the response. It is anticipated that the application of modern proteomic technologies will reveal hitherto unrecognised levels of complexity in these processes. Hopefully this will lead to the development of new therapeutic strategies to prevent long-term health implications of radiation exposure.
    MeSH term(s) Bystander Effect/radiation effects ; Extracellular Matrix/metabolism ; Extracellular Matrix/pathology ; Extracellular Matrix/radiation effects ; Fibrosis/metabolism ; Fibrosis/pathology ; Humans ; Hypoxia/metabolism ; Hypoxia/pathology ; Intercellular Signaling Peptides and Proteins/metabolism ; Neoplasms/metabolism ; Neoplasms/pathology ; Neoplasms/radiotherapy ; Proteolysis/radiation effects ; Proteome/analysis ; Proteome/metabolism ; Radiation Injuries/metabolism ; Radiation Injuries/pathology ; Radiation, Ionizing ; Signal Transduction/radiation effects ; Tumor Microenvironment/radiation effects ; Wound Healing/radiation effects
    Chemical Substances Intercellular Signaling Peptides and Proteins ; Proteome
    Language English
    Publishing date 2013
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 2214-8019 ; 0065-2598
    ISSN (online) 2214-8019
    ISSN 0065-2598
    DOI 10.1007/978-94-007-5896-4_3
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  5. Article ; Online: Isolation of Proteins from Extracellular Vesicles (EVs) for Mass Spectrometry-Based Proteomic Analyses.

    Subedi, Prabal / Schneider, Michael / Atkinson, Michael J / Tapio, Soile

    Methods in molecular biology (Clifton, N.J.)

    2021  Volume 2261, Page(s) 207–212

    Abstract: Extracellular vesicles (EVs) are freely circulating nano/micrometer-sized membrane-bound vesicles released by various cell types. Their cargo consists of proteins, lipids, metabolites, and different types of RNA molecules reflecting the origin of the ... ...

    Abstract Extracellular vesicles (EVs) are freely circulating nano/micrometer-sized membrane-bound vesicles released by various cell types. Their cargo consists of proteins, lipids, metabolites, and different types of RNA molecules reflecting the origin of the secreting cell type or tissue. Since the EV cargo is constantly changing in response to pathological status or different environmental stressors, it has been extensively studied in the quest for biomarkers, especially in the cancer research. Mass spectrometry (MS)-based proteome analysis is a powerful tool to elucidate the protein cargo in EVs. This chapter describes and characterizes three MS-compatible lysis methods, namely by using urea, guanidium hydrochloride, and radioimmunoprecipitation buffer for isolating proteins from EVs.
    MeSH term(s) Analytic Sample Preparation Methods ; Animals ; Cells, Cultured ; Exosomes/metabolism ; Guanidine/chemistry ; Humans ; Immunoprecipitation ; Mass Spectrometry ; Proteins/isolation & purification ; Proteomics ; Urea/chemistry
    Chemical Substances Proteins ; Urea (8W8T17847W) ; Guanidine (JU58VJ6Y3B)
    Language English
    Publishing date 2021-01-09
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-1186-9_12
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Quantifying telomeric lncRNAs using PNA-labelled RNA-Flow FISH (RNA-Flow).

    González-Vasconcellos, Iria / Cobos-Fernández, María A / Atkinson, Michael J / Fernandez-Piqueras, José / Santos, Javier

    Communications biology

    2022  Volume 5, Issue 1, Page(s) 513

    Abstract: Here we present a method to detect and quantify long non-coding RNAs, in particular those related to telomeres. By coupling the specificity of a peptide nucleic acid (PNA) probe with flow cytometry we have quantified cellular levels of TERRA and TERC ... ...

    Abstract Here we present a method to detect and quantify long non-coding RNAs, in particular those related to telomeres. By coupling the specificity of a peptide nucleic acid (PNA) probe with flow cytometry we have quantified cellular levels of TERRA and TERC lncRNAs in culture cell lines and PBMCs. This easy-to-use method appointed RNA-Flow allows reliable lncRNA quantification with broad applications in basic research and clinical diagnostics. In addition, the staining protocol presented here was proven useful for the detection and quantification of such lncRNAs on unfixed cells using confocal microscopy.
    MeSH term(s) Flow Cytometry/methods ; Peptide Nucleic Acids/genetics ; RNA, Long Noncoding/genetics ; Telomere/genetics
    Chemical Substances Peptide Nucleic Acids ; RNA, Long Noncoding
    Language English
    Publishing date 2022-05-25
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2399-3642
    ISSN (online) 2399-3642
    DOI 10.1038/s42003-022-03452-3
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  7. Article: The Chaperone Protein GRP78 Promotes Survival and Migration of Head and Neck Cancer After Direct Radiation Exposure and Extracellular Vesicle-Transfer.

    Schneider, Michael / Winkler, Klaudia / Kell, Rosemarie / Pfaffl, Michael W / Atkinson, Michael J / Moertl, Simone

    Frontiers in oncology

    2022  Volume 12, Page(s) 842418

    Abstract: Background and purpose: Increased levels of the chaperone protein GRP78 have been implicated in poorer outcomes of cancer therapy. We have therefore explored the functional connection between the expression of GRP78 and the development of ... ...

    Abstract Background and purpose: Increased levels of the chaperone protein GRP78 have been implicated in poorer outcomes of cancer therapy. We have therefore explored the functional connection between the expression of GRP78 and the development of radioresistance and metastatic behavior in HNSCC.
    Material and methods: The association between gene expression of GRP78 and survival in HNSCC patients was examined using the TCGA database. The influence of ionizing radiation on the GRP78 levels in HNSCC cell lines, their secreted extracellular vesicles (EV) and non-irradiated EV-recipient cells was investigated by Western Blot and FACS. The consequences of chemical inhibition or experimental overexpression of GRP78 on radioresistance and migration of HNSCC cells were analyzed by clonogenic survival and gap closure assays.
    Results: Elevated levels of GRP78 RNA in HNSCC correlated with poorer overall survival. Radiation increased GRP78 protein expression on the surface of HNSCC cell lines. Experimental overexpression of GRP78 increased both radioresistance and migratory potential. Chemical inhibition of GRP78 impaired cell migration. EVs were identified as a potential source of increased GRP78 content as elevated levels of surface GRP78 were found in EVs released by irradiated cells. These vesicles transferred GRP78 to non-irradiated recipient cells during co-cultivation.
    Conclusions: We have identified the chaperone protein GRP78 as a potential driver of increased radioresistance and motility in HNSCC. The uptake of GRP78-rich EVs originating from irradiated cells may contribute to a poorer prognosis through bystander effects mediated by the transfer of GRP78 to non-irradiated cells. Therefore, we consider the chaperone protein GRP78 to be an attractive target for improving radiotherapy strategies.
    Language English
    Publishing date 2022-03-01
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2649216-7
    ISSN 2234-943X
    ISSN 2234-943X
    DOI 10.3389/fonc.2022.842418
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  8. Book ; Online ; Thesis: Identification of the molecular mechanisms promoting neuroendocrine tumor formation

    Minaskan Karabid, Ninelia [Verfasser] / Atkinson, Michael J. [Akademischer Betreuer] / Uhlenhaut, Nina H. [Gutachter] / Atkinson, Michael J. [Gutachter]

    2020  

    Author's details Ninelia Minaskan Karabid ; Gutachter: Nina H. Uhlenhaut, Michael J. Atkinson ; Betreuer: Michael J. Atkinson
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language English
    Publisher Universitätsbibliothek der TU München
    Publishing place München
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  9. Book ; Online: Verbundprojekt 02NUK007 "Individuelle Strahlenempfindlichkeit und genomische Instabilität: Teilprojekt 02NUK007A "Mögliche Implikationen für Strahlensensitivität und Krebsrisiko"

    Atkinson, Michael J

    2012  

    Author's details Michael J. Atkinson
    Language German
    Size Online-Ressource (40 S., 6,15 MB), Ill., graph. Darst.
    Publisher Technische Informationsbibliothek u. Universitätsbibliothek ; Helmholtz Zentrum München, Inst. für Strahlenbiologie
    Publishing place Hannover ; Neuherberg
    Document type Book ; Online
    Note Förderkennzeichen BMBF 02NUK007A. - Verbund-Nr. 01066475 ; Unterschiede zwischen dem gedruckten Dokument und der elektronischen Ressource können nicht ausgeschlossen werden
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  10. Book: Verbundprojekt: Individuelle Strahlenempfindlichkeit und genomische Instabilität

    Atkinson, Michael J

    Teilprojekt: Einfluss von Zellstressantworten auf die Ausbildung akuter Nebenwirkungen nach Strahlentherapie ; Schlussbericht

    2014  

    Title variant Individuelle Strahlenempfindlichkeit und genomische Instabilität
    Author's details Michael J. Atkinson
    Language German
    Size 39 S., Ill., graph. Darst.
    Publisher Techn. Univ., Lehrstuhl für Strahlenbiologie
    Publishing place München
    Document type Book
    Note Förderkennzeichen BMBF 02NUK007E. - Verbund-Nr. 01066475
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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