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  1. Article ; Online: Molecular docking of bioactive compounds extracted and purified from selected medicinal plant species against covid-19 proteins and in vitro evaluation.

    Khanum, Ayesha / Bibi, Yamin / Khan, Ilham / Mustafa, Ghazala / Attia, Kotb A / Mohammed, Arif Ahmed / Yang, Seung Hwan / Qayyum, Abdul

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 3736

    Abstract: Bioactive compounds are secondary metabolites of plants. They offer diverse pharmacological properties. Peganum harmala is reported to have pharmaceutical effects like insecticidal, antitumor, curing malaria, anti-spasmodic, vasorelaxant, antihistaminic ... ...

    Abstract Bioactive compounds are secondary metabolites of plants. They offer diverse pharmacological properties. Peganum harmala is reported to have pharmaceutical effects like insecticidal, antitumor, curing malaria, anti-spasmodic, vasorelaxant, antihistaminic effect. Rosa brunonii has medicinal importance in its flower and fruits effective against different diseases and juice of leaf is reported to be applied externally to cure wounds and cuts. Dryopteris ramosa aqueous leaf extract is used to treat stomach ulcers and stomachaches. Each of these three medicinal plants have been indicated to have anticancer, antiviral, antioxidant, cytotoxic and antifungal effects but efficacy of their bioactive compounds remained unexplored. Study was aimed to explore In-vitro and In-silico anticancer, antiviral, antioxidant, cytotoxic and antifungal effects of bioactive compounds of above three medicinal plants. DPPH and ABTS assay were applied for assessment of antioxidant properties of compounds. Antibacterial properties of compounds were checked by agar well diffusion method. Brine shrimp lethality assay was performed to check cytotoxic effect of compounds. Molecular docking was conducted to investigate the binding efficacy between isolated compounds and targeted proteins. The compound isomangiferrin and tiliroside presented strong antioxidant potential 78.32% (± 0.213) and 77.77% (± 0.211) respectively in DPPH assay while harmaline showed 80.71% (± 0.072) at 200 µg/mL in ABTS assay. The compound harmine, harmaline and PH-HM 17 exhibited highest zone of inhibition 22 mm, 23 mm, 22 mm respectively against Xanthomonas while Irriflophenone-3-C-β- D-glucopyranoside showed maximum zone of inhibition 34 mm against E. coli. The compound isomangiferrin and vasicine contained strong antibacterial activity 32 mm and 22 mm respectively against S. aureus. The compound mangiferrin, astragalin, tiliroside, quercitin-3-O-rhamnoside showed maximum inhibitory zone 32 mm, 26 mm, 24 mm and 22 mm respectively against Klebsiella pneumoniae. Highest cytotoxic effect was observed by compound tiliroside i.e. 95% with LD
    MeSH term(s) Plants, Medicinal/chemistry ; Plant Extracts/chemistry ; Molecular Docking Simulation ; Antifungal Agents ; Antioxidants/chemistry ; Harmaline ; Staphylococcus aureus ; Escherichia coli ; COVID-19 ; Anti-Bacterial Agents/pharmacology ; Antiviral Agents/pharmacology ; Sulfonic Acids ; Benzothiazoles
    Chemical Substances 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid (28752-68-3) ; Plant Extracts ; Antifungal Agents ; Antioxidants ; Harmaline (CN58I4TOET) ; Anti-Bacterial Agents ; Antiviral Agents ; Sulfonic Acids ; Benzothiazoles
    Language English
    Publishing date 2024-02-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-54470-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: In-silico analysis and transformation of OsMYB48 transcription factor driven by CaMV35S promoter in model plant -

    Ahmad, Yumna / Haider, Saqlain / Iqbal, Javed / Naseer, Sana / Attia, Kotb A / Mohammed, Arif Ahmed / Fiaz, Sajid / Mahmood, Tariq

    GM crops & food

    2024  Volume 15, Issue 1, Page(s) 130–149

    Abstract: Global crop yield has been affected by a number of abiotic stresses. Heat, salinity, and drought stress are at the top of the list as serious environmental growth-limiting factors. To enhance crop productivity, molecular approaches have been used to ... ...

    Abstract Global crop yield has been affected by a number of abiotic stresses. Heat, salinity, and drought stress are at the top of the list as serious environmental growth-limiting factors. To enhance crop productivity, molecular approaches have been used to determine the key regulators affecting stress-related phenomena. MYB transcription factors (TF) have been reported as one of the promising defensive proteins against the unfavorable conditions that plants must face. Different roles of MYB TFs have been suggested such as regulation of cellular growth and differentiation, hormonal signaling, mediating abiotic stress responses, etc. To gain significant insights, a comprehensive in-silico analysis of OsMYB TF was carried out in comparison with 21 dicot MYB TFs and 10 monocot MYB TFs. Their chromosomal location, gene structure, protein domain, and motifs were analyzed. The phylogenetic relationship was also studied, which resulted in the classification of proteins into four basic groups: groups A, B, C, and D. The protein motif analysis identified several conserved sequences responsible for cellular activities. The gene structure analysis suggested that proteins that were present in the same class, showed similar intron-exon structures. Promoter analysis revealed major cis-acting elements that were found to be responsible for hormonal signaling and initiating a response to abiotic stress and light-induced mechanisms. The transformation of OsMYB TF into tobacco was carried out using the
    MeSH term(s) Transcription Factors/genetics ; Transcription Factors/metabolism ; Nicotiana/genetics ; Phylogeny ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Gene Expression Regulation, Plant ; Crops, Agricultural/genetics ; Stress, Physiological/genetics
    Chemical Substances Transcription Factors ; Plant Proteins
    Language English
    Publishing date 2024-03-29
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2706099-8
    ISSN 2164-5701 ; 2164-5701
    ISSN (online) 2164-5701
    ISSN 2164-5701
    DOI 10.1080/21645698.2024.2334476
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Genome-wide analysis and expression divergence of

    Parveen, Kauser / Saddique, Muhammad Abu Bakar / Waqas, Muhammad Umair / Attia, Kotb A / Rizwan, Muhammad / Abushady, Asmaa M / Shamsi, Imran Haider

    Functional plant biology : FPB

    2024  Volume 51

    Abstract: Chickpea (Cicer arietinum ) is a grain crop that is an important source of protein, vitamins, carbohydrates and minerals. It is highly sensitive to salt stress, and salt damage to cellular homeostasis and protein folding affects production. Plants have ... ...

    Abstract Chickpea (Cicer arietinum ) is a grain crop that is an important source of protein, vitamins, carbohydrates and minerals. It is highly sensitive to salt stress, and salt damage to cellular homeostasis and protein folding affects production. Plants have several mechanisms to prevent cellular damages under abiotic stresses, such as proteins in the endoplasmic reticulum (protein isulfide somerases (PDIs) and PDI-like proteins), which help prevent the build-up of mis-folded proteins that are damaged under abiotic stresses. In this study, we completed initial comprehensive genome-wide analysis of the chickpea PDI gene family. We found eight PDI genes are distributed on six out of eight chromosomes. Two pairs of paralogous genes were found to have segmental duplications. The phylogenetic analysis showed that the PDI s have a high degree of homology in C. arietinum, Cicer reticulatum, Lens culinaris, Phaseolus acutifolius, Pisum sativum and Oryza sativa . The gene structure analysis displayed that CaPDI1-CaPDI8 have 9-12 exons except for CaPDI5 , which has 25 exons. Subcellular localisation indicated accumulation of CaPDIs in endoplasmic reticulum. Protein-conserved motifs and domain analysis demonstrated that thioredoxin domains of PDI family is present in all CaPDIs. CaPDI proteins have strong protein-protein interaction. In silico expression analysis showed that four out of eight PDI genes (CPDI2, CaPDI6, CaPDI7 and CaPDI8 ) were expressed under salt stress. Of these, expression of CaPDI2 and CaPDI8 was the highest. This work indicated that PDI genes are involved in salt stress tolerance in chickpea and the CaPDIs may be further studied for their role of inducing salt tolerance.
    MeSH term(s) Protein Disulfide-Isomerases/genetics ; Protein Disulfide-Isomerases/metabolism ; Cicer/genetics ; Cicer/metabolism ; Phylogeny ; Salt Stress/genetics ; Stress, Physiological/genetics
    Chemical Substances Protein Disulfide-Isomerases (EC 5.3.4.1)
    Language English
    Publishing date 2024-01-22
    Publishing country Australia
    Document type Journal Article
    ZDB-ID 2071582-1
    ISSN 1445-4416 ; 1445-4408
    ISSN (online) 1445-4416
    ISSN 1445-4408
    DOI 10.1071/FP23253
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: A survey of bluetongue infection in one-humped camels (Camelus Dromedarius); seroprevalence and risk factors analysis

    Selim, Abdelfattah / Alsubki, Roua A. / Albohairy, Fatima M. / Attia, Kotb A. / Kimiko, Itoh

    BMC Vet Res. 2022 Dec., v. 18, no. 1 p.322-322

    2022  

    Abstract: Bluetongue (BT) is an insect-borne, non-contagious viral disease which affects domestic ruminants including camels and is transmitted by Culicoides spp. Clinical symptoms of BT are typically seen in sheep, although subclinical BT infections are mostly ... ...

    Abstract Bluetongue (BT) is an insect-borne, non-contagious viral disease which affects domestic ruminants including camels and is transmitted by Culicoides spp. Clinical symptoms of BT are typically seen in sheep, although subclinical BT infections are mostly seen in cattle, goats, and camelids. The goal of the present study was to evaluate the sero-prevalence of Bluetongue virus (BTV) in camels from some governorates in Egypt’s southern and northern regions, as well as the infection’s potential risk factors. During 2020–2021, a cross sectional study was conducted to screen presence of anti-BTV antibodies in 400 serum samples, which were collected randomly from camels, examined using competitive enzyme-linked immunosorbent assay (cELISA). The sera of 102 out of 400 camels tested positive for BTV, representing a frequency of 25.5%. Moreover, the odds of sero-positivity were higher among camels living in Aswan (OR = 5.33, 95%CI: 2.35–12.11), especially in females (OR = 2.63, 95%CI = 1.44–4.09) during summer season (OR = 2.40, 95%CI = 1.20–4.81). Furthermore, the probability of getting BTV infection increased when camels were exposed to the insect vectors (OR = 1.63, 95%CI = 0.87–3.09). The high prevalence of BTV in camels in several Egyptian regions highlights the need for more epidemiological investigations of BTV infection in other ruminant species in order to better control BT disease in these regions.
    Keywords Bluetongue virus ; Camelus dromedarius ; Culicoides ; blood serum ; bluetongue ; cattle ; cross-sectional studies ; enzyme-linked immunosorbent assay ; insects ; risk ; seroprevalence ; sheep ; summer ; surveys ; Egypt
    Language English
    Dates of publication 2022-12
    Size p. 322.
    Publishing place BioMed Central
    Document type Article ; Online
    ZDB-ID 2191675-5
    ISSN 1746-6148
    ISSN 1746-6148
    DOI 10.1186/s12917-022-03421-2
    Database NAL-Catalogue (AGRICOLA)

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  5. Article: Cross-sectional survey on Mycobacterium avium Subsp. paratuberculosis in Dromedary Camels: Seroprevalence and risk factors

    Selim, Abdelfattah / Attia, Kotb A. / Alsubki, Roua A. / Kimiko, Itoh / Sayed-Ahmed, Mohamed Z.

    Acta tropica. 2022 Feb., v. 226

    2022  

    Abstract: Johne's disease is a chronic disease with great concern in ruminants and caused by Mycobacterium avium subsp. paratuberculosis (MAP). A cross-sectional study was conducted from February 2019 to January 2020 to estimate the prevalence of MAP infection ... ...

    Abstract Johne's disease is a chronic disease with great concern in ruminants and caused by Mycobacterium avium subsp. paratuberculosis (MAP). A cross-sectional study was conducted from February 2019 to January 2020 to estimate the prevalence of MAP infection among camels which are kept in three governorates in Nile Delta of Egypt. A total of 440 serum samples were examined by ELISA for detection of MAP antibodies. The multivariable logistic regression model was performed to determine the associated risk factors for MAP infection in examined camels. Overall, the seroprevalence of MAP infection was found to be 7.5% among examined camels. The multivariable logistic regression model was performed to determine the associated risk factors for MAP infection in examined camels. The main findings revealed that the risk of getting MAP infection increased among elder camels (>10 years old) with signs of diarrhea, having communal water source and in camels grazing in the same pasture (odds ratio >1). However, geographic location, sex and contact with cattle had not significant impact regarding to seroprevalence of MAP infection in camels. The present findings confirm presence of MAP among camels which is a potential risk factor for contamination of environment and spreading of infection. Therefore, further studies for detection of infected animals in early stage are needed beside the estimated risk factors in this study to build an efficient control program.
    Keywords Camelus dromedarius ; Mycobacterium avium subsp. paratuberculosis ; blood serum ; cattle ; chronic diseases ; cross-sectional studies ; diarrhea ; odds ratio ; paratuberculosis ; pastures ; regression analysis ; risk estimate ; risk factors ; river deltas ; seroprevalence ; Egypt
    Language English
    Dates of publication 2022-02
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 210415-5
    ISSN 1873-6254 ; 0001-706X
    ISSN (online) 1873-6254
    ISSN 0001-706X
    DOI 10.1016/j.actatropica.2021.106261
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: A survey of bluetongue infection in one-humped camels (Camelus Dromedarius); seroprevalence and risk factors analysis.

    Selim, Abdelfattah / Alsubki, Roua A / Albohairy, Fatima M / Attia, Kotb A / Kimiko, Itoh

    BMC veterinary research

    2022  Volume 18, Issue 1, Page(s) 322

    Abstract: Bluetongue (BT) is an insect-borne, non-contagious viral disease which affects domestic ruminants including camels and is transmitted by Culicoides spp. Clinical symptoms of BT are typically seen in sheep, although subclinical BT infections are mostly ... ...

    Abstract Bluetongue (BT) is an insect-borne, non-contagious viral disease which affects domestic ruminants including camels and is transmitted by Culicoides spp. Clinical symptoms of BT are typically seen in sheep, although subclinical BT infections are mostly seen in cattle, goats, and camelids. The goal of the present study was to evaluate the sero-prevalence of Bluetongue virus (BTV) in camels from some governorates in Egypt's southern and northern regions, as well as the infection's potential risk factors. During 2020-2021, a cross sectional study was conducted to screen presence of anti-BTV antibodies in 400 serum samples, which were collected randomly from camels, examined using competitive enzyme-linked immunosorbent assay (cELISA). The sera of 102 out of 400 camels tested positive for BTV, representing a frequency of 25.5%. Moreover, the odds of sero-positivity were higher among camels living in Aswan (OR = 5.33, 95%CI: 2.35-12.11), especially in females (OR = 2.63, 95%CI = 1.44-4.09) during summer season (OR = 2.40, 95%CI = 1.20-4.81). Furthermore, the probability of getting BTV infection increased when camels were exposed to the insect vectors (OR = 1.63, 95%CI = 0.87-3.09). The high prevalence of BTV in camels in several Egyptian regions highlights the need for more epidemiological investigations of BTV infection in other ruminant species in order to better control BT disease in these regions.
    MeSH term(s) Animals ; Antibodies, Viral/blood ; Bluetongue/epidemiology ; Bluetongue virus/immunology ; Bluetongue virus/isolation & purification ; Camelus/virology ; Cross-Sectional Studies ; Egypt/epidemiology ; Female ; Male ; Risk Factors ; Seroepidemiologic Studies
    Chemical Substances Antibodies, Viral
    Language English
    Publishing date 2022-08-22
    Publishing country England
    Document type Journal Article
    ZDB-ID 2191675-5
    ISSN 1746-6148 ; 1746-6148
    ISSN (online) 1746-6148
    ISSN 1746-6148
    DOI 10.1186/s12917-022-03421-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Prevalence, antimicrobial susceptibilities and risk factors of Methicillin resistant Staphylococcus aureus (MRSA) in dairy bovines.

    Selim, Abdelfattah / Kelis, Khalid / AlKahtani, Muneera D F / Albohairy, Fatima M / Attia, Kotb A

    BMC veterinary research

    2022  Volume 18, Issue 1, Page(s) 293

    Abstract: Staphylococcus aureus is a common mastitis pathogen in dairy cows, and methicillin-resistant S. aureus (MRSA) has been found in dairy farms all over the world. The study carried out on bovines from three governorates in Egypt, with the goal of ... ...

    Abstract Staphylococcus aureus is a common mastitis pathogen in dairy cows, and methicillin-resistant S. aureus (MRSA) has been found in dairy farms all over the world. The study carried out on bovines from three governorates in Egypt, with the goal of determining the prevalence of MRSA in positive milk samples of subclinical mastitis, performing an antibiotic susceptibility test against MRSA isolates and determining the risk factors associated with MRSA. A total of 350 quarter milk samples (n = 200 mixed breed cow; n = 150 water buffalo) were collected and examined for subclinical mastitis using the California mastitis test (CMT) before being exposed to standard microbiological procedures for S. aureus isolation. The disc diffusion method was used to phenotypically analyse the positive S. aureus isolates for MRSA, which was verified by a PCR assay targeting the mecA gene. According to the findings of the study, 41.4% (145/350) milk samples were positive based on CMT, while 35.7% (125/350) of positive samples identified as MRSA based on PCR assay. However, the obtained results revealed non-significant disparity between cattle and buffalo and all predicted risk factors were strongly associated with prevalence of subclinical mastitis. The in-vitro antibiotic susceptibility test revealed that cefoxitin was completely resistant, whereas linezolid, ciprofloxacin, and trimethoprim + sulphamethoxazole were sensitive against the MRSA isolates. The relevance of S. aureus to public health, as well as the development of resistance to antibiotics like methicillin, needs ongoing testing of antimicrobial medications against MRSA isolates.
    MeSH term(s) Animals ; Anti-Bacterial Agents/pharmacology ; Cattle ; Cattle Diseases/epidemiology ; Female ; Mastitis, Bovine/epidemiology ; Mastitis, Bovine/microbiology ; Methicillin-Resistant Staphylococcus aureus ; Microbial Sensitivity Tests/veterinary ; Milk/microbiology ; Prevalence ; Risk Factors ; Staphylococcal Infections/epidemiology ; Staphylococcal Infections/microbiology ; Staphylococcal Infections/veterinary ; Staphylococcus aureus
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2022-07-29
    Publishing country England
    Document type Journal Article
    ZDB-ID 2191675-5
    ISSN 1746-6148 ; 1746-6148
    ISSN (online) 1746-6148
    ISSN 1746-6148
    DOI 10.1186/s12917-022-03389-z
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  8. Article: The first study on the seroprevalence of

    Selim, Abdelfattah / Attia, Kotb A / Alsubki, Roua A / Albohairy, Fatima / Kimiko, Itoh / Said, Mourad Ben

    Veterinary world

    2022  Volume 15, Issue 5, Page(s) 1221–1227

    Abstract: Background and aim: Ovine anaplasmosis is a rickettsial disease caused by : Materials and methods: A cross-sectional study was performed to investigate the seroprevalence of : Results: Overall, the seroprevalence of anti-: Conclusion: Age, ... ...

    Abstract Background and aim: Ovine anaplasmosis is a rickettsial disease caused by
    Materials and methods: A cross-sectional study was performed to investigate the seroprevalence of
    Results: Overall, the seroprevalence of anti-
    Conclusion: Age, animal husbandry, acaricide use, tick infestation, and contact with cattle were the primary risk factors for
    Language English
    Publishing date 2022-05-20
    Publishing country India
    Document type Journal Article
    ZDB-ID 2456277-4
    ISSN 2231-0916 ; 0972-8988
    ISSN (online) 2231-0916
    ISSN 0972-8988
    DOI 10.14202/vetworld.2022.1221-1227
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Sero-survey of bovine herpes virus-1 in dromedary camels and associated risk factors.

    Selim, Abdelfattah / Shoulah, Salma / Alsubki, Roua A / Albohairy, Fatima M / Attia, Kotb A / Kimiko, Itoh

    BMC veterinary research

    2022  Volume 18, Issue 1, Page(s) 362

    Abstract: Infectious bovine rhinotracheitis (IBR) is a major animal health hazard in many countries throughout the world, caused by bovine herpesvirus-1 (BoHV-1). The study's goal was to evaluate the prevalence of BoHV-1 seropositivity among dromedary camels in ... ...

    Abstract Infectious bovine rhinotracheitis (IBR) is a major animal health hazard in many countries throughout the world, caused by bovine herpesvirus-1 (BoHV-1). The study's goal was to evaluate the prevalence of BoHV-1 seropositivity among dromedary camels in three governorates in northern Egypt, as well as to identify risk variables related with BoHV-1 seropositivity. A total of 321 blood samples were collected randomly from dromedary camels living in the selected governorates and examined for presence of BoHV-1 antibody using ELISA test. The overall seroprevalence of BoHV-1 among examined camels was 5.92% (95%CI: 3.82-9.06). Univariable analysis confirmed that the significant association (P < 0.05) between sex, history of abortion, contact with small ruminants and herd size and BoHV-1 seropositivity. Using multiple logistic regression analysis, the following risk factors were identified to be related with the presence of BoHV-1 infection: sex (OR = 2.54, 95%CI: 0.63-10.22), history of abortion (OR = 4.16, 95%CI: 1.30-13.27), contact with small ruminants (OR = 5.61, 95%CI: 1.67-18.80) and large herd size (OR = 10.52, 95%CI: 2.46-44.91). This study estimated the disease's seroprevalence in Egyptian dromedary camels, implying that camels could act as a BoHV-1 reservoir for transmission to other species.
    MeSH term(s) Abortion, Veterinary ; Animals ; Camelus ; Cattle ; Cattle Diseases ; Female ; Herpesvirus 1, Bovine ; Pregnancy ; Risk Factors ; Seroepidemiologic Studies
    Language English
    Publishing date 2022-09-30
    Publishing country England
    Document type Journal Article
    ZDB-ID 2191675-5
    ISSN 1746-6148 ; 1746-6148
    ISSN (online) 1746-6148
    ISSN 1746-6148
    DOI 10.1186/s12917-022-03448-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Molecular epidemiological survey, genetic characterization and phylogenetic analysis of Anaplasma ovis infecting sheep in Northern Egypt.

    Said, Mourad Ben / Attia, Kotb A / Alsubki, Roua A / Mohamed, Arif A / Kimiko, Itoh / Selim, Abdelfattah

    Acta tropica

    2022  Volume 229, Page(s) 106370

    Abstract: Anaplasma ovis is the most common etiologic agent of ovine anaplasmosis, mainly transmitted by ticks. The present study aimed to determine the molecular prevalence of A. ovis in sheep from Egypt and assessed the associated risk factors. The study was ... ...

    Abstract Anaplasma ovis is the most common etiologic agent of ovine anaplasmosis, mainly transmitted by ticks. The present study aimed to determine the molecular prevalence of A. ovis in sheep from Egypt and assessed the associated risk factors. The study was conducted, between January and December 2020, in four governorates situated in Northern Egypt. Blood samples from 355 asymptomatic sheep were collected and examined by the use of PCR specific to A. ovis. Diversity analysis and phylogenetic study based on partial msp4 gene sequence were performed on revealed A. ovis DNA. Overall, the molecular prevalence rate of A. ovis was 15.5% and the highest rate was observed in Kafr ElSheikh governorate (16.8%). Statistical analysis revealed that A. ovis infection was significantly related to sheep gender and to tick infestation. The risk factors that were found to be associated with A. ovis infection in exposed sheep were: female sex (OR=2.6, 95%CI: 1.13-6.12), and infestation with ticks (OR=2.1, 95%CI: 1.11-3.79). The analysis of A. ovis msp4 sequences revealed two different genotypes classified in the Old World sub-cluster with other Egyptian isolates. Investigation on prevalence, risk factors and genetic variability of A. ovis in sheep reported in this study is important for the implementation of control programs. Further studies are needed to determine the vectors and reservoirs of A. ovis in Egyptian small ruminants and to identify the real economic impact of A. ovis infection on the country.
    MeSH term(s) Anaplasma ovis/genetics ; Anaplasmosis/epidemiology ; Animals ; Egypt/epidemiology ; Female ; Goats ; Phylogeny ; Sheep ; Sheep Diseases/epidemiology
    Language English
    Publishing date 2022-02-12
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 210415-5
    ISSN 1873-6254 ; 0001-706X
    ISSN (online) 1873-6254
    ISSN 0001-706X
    DOI 10.1016/j.actatropica.2022.106370
    Database MEDical Literature Analysis and Retrieval System OnLINE

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