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  1. Article ; Online: Novel simultaneous determination of alliin and allicin in Allium sp. using digital subtraction HPTLC.

    Bosca, Bogdan / Mot, Augustin C

    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    2023  Volume 1222, Page(s) 123700

    Abstract: Allicin is a major thiosulfinate found in garlic and other Allium sp. and it is responsible for their pungent aroma. It is formed during the tissue lysis of garlic, by the initial action of alliinase upon alliin, the major cysteine sulfoxide. ... ...

    Abstract Allicin is a major thiosulfinate found in garlic and other Allium sp. and it is responsible for their pungent aroma. It is formed during the tissue lysis of garlic, by the initial action of alliinase upon alliin, the major cysteine sulfoxide. Simultaneous detection of these two analytes is usually performed using HPLC. Contrary to the most important phytoconstituents in other samples, allicin is scarcely detected using the simple HPTLC technique, due to challenges caused by its unique structure, despite its simplicity and high needs in the analytical monitoring of the Allium sp. In this work, a cost-effective, simple, sensitive and accurate method was developed for the determination of allicin together with alliin, using HPTLC. Allicin is quickly pre-derivatised with cysteine in excess to the stable S-allylmercaptocysteine that is then simultaneously detected with alliin, using ninhydrin reagent. The method was validated in terms of accuracy (recoveries of 90-120 %), precision (RSD% of 4-12 %), selectivity, robustness, peak purity and limit of detection (LOD = 0.05 μg/band for allicin and LOD = 0.10 μg/band for alliin). The method was successfully applied using real Allium sp. samples and the results were in good agreement with HPLC data.
    MeSH term(s) Cysteine ; Garlic/chemistry ; Sulfinic Acids ; Disulfides ; Antioxidants
    Chemical Substances alliin (7I4L2D0E9G) ; Cysteine (K848JZ4886) ; allicin (3C39BY17Y6) ; Sulfinic Acids ; Disulfides ; Antioxidants
    Language English
    Publishing date 2023-04-05
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1180823-8
    ISSN 1873-376X ; 0378-4347 ; 1570-0232 ; 1387-2273
    ISSN (online) 1873-376X
    ISSN 0378-4347 ; 1570-0232 ; 1387-2273
    DOI 10.1016/j.jchromb.2023.123700
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Four-dimensional reconstruction and characterization of bladder deformations.

    Ogier, Augustin C / Rapacchi, Stanislas / Bellemare, Marc-Emmanuel

    Computer methods and programs in biomedicine

    2023  Volume 237, Page(s) 107569

    Abstract: Background and objective: Pelvic floor disorders are prevalent diseases and patient care remains difficult as the dynamics of the pelvic floor remains poorly understood. So far, only 2D dynamic observations of straining exercises at excretion are ... ...

    Abstract Background and objective: Pelvic floor disorders are prevalent diseases and patient care remains difficult as the dynamics of the pelvic floor remains poorly understood. So far, only 2D dynamic observations of straining exercises at excretion are available in the clinics and 3D mechanical defects of pelvic organs are not well studied. In this context, we propose a complete methodology for the 3D representation of non-reversible bladder deformations during exercises, combined with a 3D representation of the location of the highest strain areas on the organ surface.
    Methods: Novel image segmentation and registration approaches have been combined with three geometrical configurations of up-to-date rapid dynamic multi-slice MRI acquisitions for the reconstruction of real-time dynamic bladder volumes.
    Results: For the first time, we proposed real-time 3D deformation fields of the bladder under strain from in-bore forced breathing exercises. The potential of our method was assessed on eight control subjects undergoing forced breathing exercises. We obtained average volume deviations of the reconstructed dynamic volume of bladders around 2.5% and high registration accuracy with mean distance values of 0.4 ± 0.3 mm and Hausdorff distance values of 2.2 ± 1.1 mm.
    Conclusions: The proposed framework provides proper 3D+t spatial tracking of non-reversible bladder deformations. This has immediate applicability in clinical settings for a better understanding of pelvic organ prolapse pathophysiology. This work can be extended to patients with cavity filling or excretion problems to better characterize the severity of pelvic floor pathologies or to be used for preoperative surgical planning.
    MeSH term(s) Humans ; Urinary Bladder/diagnostic imaging ; Magnetic Resonance Imaging/methods ; Pelvic Floor/diagnostic imaging ; Pelvic Floor/pathology
    Language English
    Publishing date 2023-04-29
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 632564-6
    ISSN 1872-7565 ; 0169-2607
    ISSN (online) 1872-7565
    ISSN 0169-2607
    DOI 10.1016/j.cmpb.2023.107569
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Chromatographic Determination of Total Selenium in Biofortified

    Bosca, Bogdan M / Mot, Augustin C

    Molecules (Basel, Switzerland)

    2021  Volume 26, Issue 21

    Abstract: Herein, a method based on selective piazselenol formation is applied for total selenium determination in ... ...

    Abstract Herein, a method based on selective piazselenol formation is applied for total selenium determination in biofortified
    MeSH term(s) Allium/chemistry ; Azoles/chemistry ; Drug Compounding ; Molecular Structure ; Organoselenium Compounds/chemistry ; Selenium/analysis ; Solid Phase Microextraction
    Chemical Substances Azoles ; Organoselenium Compounds ; piaselenole (273-15-4) ; Selenium (H6241UJ22B)
    Language English
    Publishing date 2021-11-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules26216730
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: The Preperitoneal Space in Hernia Repair.

    Lorenz, A / Augustin, C / Konschake, M / Gehwolf, P / Henninger, B / Augustin, F / Öfner, D

    Frontiers in surgery

    2022  Volume 9, Page(s) 869731

    Abstract: The preperitoneal spaces relevant for incisional hernia repair and minimally invasive groin hernia repair are described in terms of surgical anatomy. Emphasis is put on the transversalis fascia and the urogenital fascia and its extensions, the ... ...

    Abstract The preperitoneal spaces relevant for incisional hernia repair and minimally invasive groin hernia repair are described in terms of surgical anatomy. Emphasis is put on the transversalis fascia and the urogenital fascia and its extensions, the vesicoumbilical fascia, and the spermatic sheath of Stoppa procedure. Steps in hernia surgery where these structures are relevant are reviewed.
    Language English
    Publishing date 2022-05-30
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2773823-1
    ISSN 2296-875X
    ISSN 2296-875X
    DOI 10.3389/fsurg.2022.869731
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Redox Reactivity of Nonsymbiotic Phytoglobins towards Nitrite.

    Zagrean-Tuza, Cezara / Pato, Galaba / Damian, Grigore / Silaghi-Dumitrescu, Radu / Mot, Augustin C

    Molecules (Basel, Switzerland)

    2024  Volume 29, Issue 6

    Abstract: Nonsymbiotic phytoglobins (nsHbs) are a diverse superfamily of hemoproteins grouped into three different classes (1, 2, and 3) based on their sequences. Class 1 Hb are expressed under hypoxia, osmotic stress, and/or nitric oxide exposure, while class 2 ... ...

    Abstract Nonsymbiotic phytoglobins (nsHbs) are a diverse superfamily of hemoproteins grouped into three different classes (1, 2, and 3) based on their sequences. Class 1 Hb are expressed under hypoxia, osmotic stress, and/or nitric oxide exposure, while class 2 Hb are induced by cold stress and cytokinins. Both are mainly six-coordinated. The deoxygenated forms of the class 1 and 2 nsHbs from
    MeSH term(s) Animals ; Nitrites/chemistry ; Nitric Oxide/metabolism ; Hemoglobins/chemistry ; Nitrite Reductases/chemistry ; Hypoxia ; Arabidopsis/metabolism ; Oxidation-Reduction ; Mammals/metabolism
    Chemical Substances Nitrites ; Nitric Oxide (31C4KY9ESH) ; Hemoglobins ; Nitrite Reductases (EC 1.7.-)
    Language English
    Publishing date 2024-03-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules29061200
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  6. Article ; Online: Effect of knee joint angle on vastus medialis and vastus lateralis rigidity during isometric submaximal voluntary knee extensions.

    Bouvier, Jérémie / Ogier, Augustin C / Martin, Cyril / Fouré, Alexandre

    Journal of electromyography and kinesiology : official journal of the International Society of Electrophysiological Kinesiology

    2023  Volume 73, Page(s) 102826

    Abstract: The use of shear wave elastography during voluntary contraction has enabled the non-invasive assessment of load sharing strategies between agonist muscles. However, the change in joint angle and voluntary contraction intensity can modify contribution ... ...

    Abstract The use of shear wave elastography during voluntary contraction has enabled the non-invasive assessment of load sharing strategies between agonist muscles. However, the change in joint angle and voluntary contraction intensity can modify contribution between muscles. The aim of this study was to investigate the effect of knee joint angle on the local mechanical properties of the vastus medialis (VM) and the vastus lateralis (VL) during isometric submaximal voluntary contractions from shear wave elastography mapping. The VM and VL Young's modulus at rest and during constant isometric submaximal voluntary contractions (i.e., 25%, 50% and 75% of maximal voluntary contraction [MVC]) were assessed for two knee angles (50° and 100° | knee fully extended = 0°) in twelve participants. No significant difference was found in the VM Young's modulus among all torque levels and knee angles (p > 0.05). VL Young's modulus was significantly higher at 25% MVC for a knee angle of 100° than at 75% MVC for the same knee angle and was greater at 25% MVC for a knee angle of 100° than for 50° (p < 0.05). In contrast to the VM, the contribution of the VL to the knee joint torque production during isometric voluntary contraction appears to depend on the muscle length and the relative knee extension torque level.
    MeSH term(s) Humans ; Quadriceps Muscle/diagnostic imaging ; Quadriceps Muscle/physiology ; Muscle, Skeletal/physiology ; Electromyography ; Knee/physiology ; Knee Joint/physiology ; Isometric Contraction/physiology ; Torque
    Language English
    Publishing date 2023-09-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 1073161-1
    ISSN 1873-5711 ; 1050-6411
    ISSN (online) 1873-5711
    ISSN 1050-6411
    DOI 10.1016/j.jelekin.2023.102826
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Chromatographic Determination of Total Selenium in Biofortified Allium sp. following Piazselenol Formation and Micro-Solid-Phase Extraction

    Bogdan M. Bosca / Augustin C. Mot

    Molecules, Vol 26, Iss 6730, p

    2021  Volume 6730

    Abstract: Herein, a method based on selective piazselenol formation is applied for total selenium determination in biofortified Allium species. Piazselenol is formed by reacting Se(IV) with an aromatic diamine, namely 4-nitro-1,2-phenylenediamine, in acidic medium. ...

    Abstract Herein, a method based on selective piazselenol formation is applied for total selenium determination in biofortified Allium species. Piazselenol is formed by reacting Se(IV) with an aromatic diamine, namely 4-nitro-1,2-phenylenediamine, in acidic medium. Samples were digested in a nitric acid/hydrogen peroxide open system, followed by selenate reduction in hydrochloric acid. Reaction conditions were optimized in terms of pH, temperature, reaction time, and other auxiliary reagents for interference removal, namely, EDTA and hydroxylamine. For the extraction of the selectively formed 4-nitro-piazselenol, micro-solid-phase extraction (μSPE) was applied, and the analysis and detection of the corresponding complex was performed by HPLC coupled with DAD. An external standard calibration curve was developed (R 2 = 0.9994) with good sensitivity, and was used to calculate the total selenium content from several Allium plants material, with good intermediate precision (RSD% < 16%). The accuracy of the method was evaluated using both, a comparison with an accepted reference method from our previously published data, as well as three certified reference material with recoveries between 84–126%. The limit of detection was determined to be 0.35 μg/g (in solids) and 1.1 μg/L (in solution), while the limit of quantification was 1.07 μg/g and 3.4 μg/L (in solution). Using the proposed method, selenium content can be quickly and accurately determined in several types of samples. In addition, this study present experimental conditions for overcoming the interferences that might be encountered in selenium determination using piazselenol.
    Keywords selenium ; Allium ; piazselenol ; micro-solid-phase extraction ; Organic chemistry ; QD241-441
    Subject code 540
    Language English
    Publishing date 2021-11-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Book ; Online: Three-dimensional reconstruction and characterization of bladder deformations

    Ogier, Augustin C. / Rapacchi, Stanislas / Bellemare, Marc-Emmanuel

    2023  

    Abstract: Background and Objective: Pelvic floor disorders are prevalent diseases and patient care remains difficult as the dynamics of the pelvic floor remains poorly known. So far, only 2D dynamic observations of straining exercises at excretion are available in ...

    Abstract Background and Objective: Pelvic floor disorders are prevalent diseases and patient care remains difficult as the dynamics of the pelvic floor remains poorly known. So far, only 2D dynamic observations of straining exercises at excretion are available in the clinics and the understanding of three-dimensional pelvic organs mechanical defects is not yet achievable. In this context, we proposed a complete methodology for the 3D representation of the non-reversible bladder deformations during exercises, directly combined with synthesized 3D representation of the location of the highest strain areas on the organ surface. Methods: Novel image segmentation and registration approaches have been combined with three geometrical configurations of up-to-date rapid dynamic multi-slices MRI acquisition for the reconstruction of real-time dynamic bladder volumes. Results: For the first time, we proposed real-time 3D deformation fields of the bladder under strain from in-bore forced breathing exercises. The potential of our method was assessed on eight control subjects undergoing forced breathing exercises. We obtained average volume deviation of the reconstructed dynamic volume of bladders around 2.5\% and high registration accuracy with mean distance values of 0.4 $\pm$ 0.3 mm and Hausdorff distance values of 2.2 $\pm$ 1.1 mm. Conclusions: Immediately transferable to the clinics with rapid acquisitions, the proposed framework represents a real advance in the field of pelvic floor disorders as it provides, for the first time, a proper 3D+t spatial tracking of bladder non-reversible deformations. This work is intended to be extended to patients with cavities filling and excretion to better characterize the degree of severity of pelvic floor pathologies for diagnostic assistance or in preoperative surgical planning.

    Comment: 17 pages, 7 figures, full article paper
    Keywords Computer Science - Computer Vision and Pattern Recognition
    Subject code 670
    Publishing date 2023-01-18
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: The trophic ecology of an invasive predator in a novel ecosystem: Green sunfish Lepomis cyanellus in a restored urban stream

    Babbington, Brittany A. / Engman, Augustin C. / Clark, Z. Winston / Ramírez, Alonso

    Food Webs. 2023 Sept., v. 36 p.e00292-

    2023  

    Abstract: Rapid urbanization will increase the number of novel stream ecosystems in the Southeastern United States. The green sunfish (Lepomis cyanellus) is a globally widespread, invasive species that is particularly well-adapted to urban stream conditions. The ... ...

    Abstract Rapid urbanization will increase the number of novel stream ecosystems in the Southeastern United States. The green sunfish (Lepomis cyanellus) is a globally widespread, invasive species that is particularly well-adapted to urban stream conditions. The trophic ecology of green sunfish is understudied, especially in the novel ecosystems where they appear to thrive. We assessed predation by green sunfish in the food web of a heavily engineered and restored urban stream in Raleigh, North Carolina. We sampled fish species composition, size structure, abundance, the benthic macroinvertebrate assemblage, and fish diets during two seasons. Green sunfish was the sole species inhabiting the study stream, with the exception of a single goldfish. The population size structure indicated potential overcrowding in our study ecosystem. Chironomidae was the most common taxa in both the benthic macroinvertebrate assemblage and in fish diets during the summer. We observed a seasonal shift in diets to lesser prey-specific abundance of Chironomidae and a greater overall abundance of terrestrial prey from summer to winter. Green sunfish can persist in small restored urban streams of the Southeast US where virtually no other fish occur, and they utilize benthic invertebrates and terrestrial prey as resources.
    Keywords Chironomidae ; Lepomis cyanellus ; ecosystems ; food webs ; goldfish ; invasive species ; macroinvertebrates ; population size ; predation ; species diversity ; streams ; summer ; urbanization ; North Carolina ; Urban stream ; Trophic ecology
    Language English
    Dates of publication 2023-09
    Publishing place Elsevier Inc.
    Document type Article ; Online
    ISSN 2352-2496
    DOI 10.1016/j.fooweb.2023.e00292
    Database NAL-Catalogue (AGRICOLA)

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  10. Article ; Online: Multiple miliary osteoma cutis treatment response to Q-switched Nd:YAG laser

    Augustin C. Barolet / Ivan V. Litvinov / Daniel Barolet

    SAGE Open Medical Case Reports, Vol

    A case report

    2020  Volume 8

    Abstract: Facial multiple miliary osteoma cutis is a variant of osteoma cutis usually occurring in women with a previous history of acne vulgaris. Successful ablative laser treatment has been reported using both CO2 and Er:YAG lasers among other invasive treatment ...

    Abstract Facial multiple miliary osteoma cutis is a variant of osteoma cutis usually occurring in women with a previous history of acne vulgaris. Successful ablative laser treatment has been reported using both CO2 and Er:YAG lasers among other invasive treatment modalities, like surgical removal. We report a patient with biopsy-proven facial multiple miliary osteoma cutis responding to non-ablative Q-switched Nd:YAG laser therapy. The patient had excellent cosmesis without textural changes or hypopigmentation despite her Asian background after three sessions over 6 months. Multiple miliary osteoma cutis is therefore now amenable to non-surgical non-ablative therapy by using Q-switched Nd:YAG laser therapy reducing the risk of textural changes and hypopigmentation, especially in dark complexion and high-risk individuals. To our knowledge, this treatment approach has not been previously reported.
    Keywords Medicine (General) ; R5-920
    Language English
    Publishing date 2020-03-01T00:00:00Z
    Publisher SAGE Publishing
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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