Article ; Online: The proteomic landscape of genome-wide genetic perturbations.
2023 Volume 186, Issue 9, Page(s) 2018–2034.e21
Abstract: Functional genomic strategies have become fundamental for annotating gene function and regulatory networks. Here, we combined functional genomics with proteomics by quantifying protein abundances in a genome-scale knockout library in Saccharomyces ... ...
| Abstract | Functional genomic strategies have become fundamental for annotating gene function and regulatory networks. Here, we combined functional genomics with proteomics by quantifying protein abundances in a genome-scale knockout library in Saccharomyces cerevisiae, using data-independent acquisition mass spectrometry. We find that global protein expression is driven by a complex interplay of (1) general biological properties, including translation rate, protein turnover, the formation of protein complexes, growth rate, and genome architecture, followed by (2) functional properties, such as the connectivity of a protein in genetic, metabolic, and physical interaction networks. Moreover, we show that functional proteomics complements current gene annotation strategies through the assessment of proteome profile similarity, protein covariation, and reverse proteome profiling. Thus, our study reveals principles that govern protein expression and provides a genome-spanning resource for functional annotation. |
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| MeSH term(s) | Proteomics/methods ; Proteome/metabolism ; Genomics/methods ; Genome ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism |
| Chemical Substances | Proteome |
| Language | English |
| Publishing date | 2023-04-19 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 187009-9 |
| ISSN | 1097-4172 ; 0092-8674 |
| ISSN (online) | 1097-4172 |
| ISSN | 0092-8674 |
| DOI | 10.1016/j.cell.2023.03.026 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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