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  1. Article: Assessing depth sensitivity in laser interferometry speckle visibility spectroscopy (iSVS) through source-to-detector distance variation and cerebral blood flow monitoring in humans and rabbits.

    Mahler, Simon / Huang, Yu Xi / Liang, Mingshu / Avalos, Alan / Tyszka, Julian M / Mertz, Jerome / Yang, Changhuei

    Biomedical optics express

    2023  Volume 14, Issue 9, Page(s) 4964–4978

    Abstract: Recently, speckle visibility spectroscopy (SVS) was non-invasively applied on the head to monitor cerebral blood flow. The technique, using a multi-pixel detecting device (e.g., camera), allows the detection of a larger number of speckles, increasing the ...

    Abstract Recently, speckle visibility spectroscopy (SVS) was non-invasively applied on the head to monitor cerebral blood flow. The technique, using a multi-pixel detecting device (e.g., camera), allows the detection of a larger number of speckles, increasing the proportion of light that is detected. Due to this increase, it is possible to collect light that has propagated deeper through the brain. As a direct consequence, cerebral blood flow can be monitored. However, isolating the cerebral blood flow from the other layers, such as the scalp or skull components, remains challenging. In this paper, we report our investigations on the depth-sensitivity of laser interferometry speckle visibility spectroscopy (iSVS). Specifically, we varied the depth of penetration of the laser light into the head by tuning the source-to-detector distance, and identified the transition point at which cerebral blood flow in humans and rabbits starts to be detected.
    Language English
    Publishing date 2023-08-29
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2572216-5
    ISSN 2156-7085
    ISSN 2156-7085
    DOI 10.1364/BOE.498815
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: The actin-regulatory protein Hem-1 is essential for alveolar macrophage development.

    Suwankitwat, Nutthakarn / Libby, Stephen / Liggitt, H Denny / Avalos, Alan / Ruddell, Alanna / Rosch, Jason W / Park, Heon / Iritani, Brian M

    The Journal of experimental medicine

    2021  Volume 218, Issue 4

    Abstract: Hematopoietic protein-1 (Hem-1) is a hematopoietic cell-specific actin-regulatory protein. Loss-of-function (LOF) variants in the NCKAP1L gene encoding Hem-1 have recently been found to result in primary immunodeficiency disease (PID) in humans, ... ...

    Abstract Hematopoietic protein-1 (Hem-1) is a hematopoietic cell-specific actin-regulatory protein. Loss-of-function (LOF) variants in the NCKAP1L gene encoding Hem-1 have recently been found to result in primary immunodeficiency disease (PID) in humans, characterized by recurring respiratory infections, asthma, and high mortality. However, the mechanisms of how Hem-1 variants result in PID are not known. In this study, we generated constitutive and myeloid cell-specific Nckap1l-KO mice to dissect the importance of Hem-1 in lung immunity. We found that Hem-1-deficient mice accumulated excessive surfactant and cell debris in airways (pulmonary alveolar proteinosis) due to impaired development of alveolar macrophages (AMs) and reduced expression of the AM differentiation factor Pparg. Residual Hem-1-deficient AMs shifted to a proinflammatory phenotype, and Hem-1-deficient neutrophils and monocytes failed to migrate normally. Myeloid cell-specific Hem-1-deficient mice exhibited increased morbidity following influenza A virus or Streptococcus pneumoniae challenge. These results provide potential mechanisms for how LOF variants in Hem-1 result in recurring respiratory diseases.
    MeSH term(s) Actins/metabolism ; Adaptor Proteins, Signal Transducing/genetics ; Adaptor Proteins, Signal Transducing/physiology ; Animals ; Cell Differentiation/genetics ; Cell Differentiation/immunology ; Cell Movement/genetics ; Cell Movement/immunology ; Cells, Cultured ; Disease Models, Animal ; Female ; Lung/immunology ; Macrophages, Alveolar/immunology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Monocytes/immunology ; Neutrophils/immunology ; PPAR gamma/metabolism ; Phagocytosis/genetics ; Phagocytosis/immunology ; Pulmonary Alveolar Proteinosis/immunology ; Pulmonary Alveolar Proteinosis/metabolism ; Signal Transduction/genetics ; Signal Transduction/immunology
    Chemical Substances Actins ; Adaptor Proteins, Signal Transducing ; Hem1 protein, mouse ; PPAR gamma ; Pparg protein, mouse
    Language English
    Publishing date 2021-02-18
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 218343-2
    ISSN 1540-9538 ; 0022-1007
    ISSN (online) 1540-9538
    ISSN 0022-1007
    DOI 10.1084/jem.20200472
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Hem-1 regulates protective humoral immunity and limits autoantibody production in a B cell-specific manner.

    Avalos, Alan / Tietsort, Jacob T / Suwankitwat, Nutthakarn / Woods, Jonathan D / Jackson, Shaun W / Christodoulou, Alexandra / Morrill, Christopher / Liggitt, H Denny / Zhu, Chengsong / Li, Quan-Zhen / Bui, Kevin K / Park, Heon / Iritani, Brian M

    JCI insight

    2022  Volume 7, Issue 9

    Abstract: Hematopoietic protein-1 (Hem-1) is a member of the actin-regulatory WASp family verprolin homolog (WAVE) complex. Loss-of-function variants in the NCKAP1L gene encoding Hem-1 were recently discovered to result in primary immunodeficiency disease (PID) in ...

    Abstract Hematopoietic protein-1 (Hem-1) is a member of the actin-regulatory WASp family verprolin homolog (WAVE) complex. Loss-of-function variants in the NCKAP1L gene encoding Hem-1 were recently discovered to result in primary immunodeficiency disease (PID) in children, characterized by poor specific Ab responses, increased autoantibodies, and high mortality. However, the mechanisms of how Hem-1 deficiency results in PID are unclear. In this study, we utilized constitutive and B cell-specific Nckap1l-KO mice to dissect the importance of Hem-1 in B cell development and functions. B cell-specific disruption of Hem-1 resulted in reduced numbers of recirculating follicular (FO), marginal zone (MZ), and B1 B cells. B cell migration in response to CXCL12 and -13 were reduced. T-independent Ab responses were nearly abolished, resulting in failed protective immunity to Streptococcus pneumoniae challenge. In contrast, T-dependent IgM and IgG2c, memory B cell, and plasma cell responses were more robust relative to WT control mice. B cell-specific Hem-1-deficient mice had increased autoantibodies against multiple autoantigens, and this correlated with hyperresponsive BCR signaling and increased representation of CD11c+T-bet+ age-associated B cell (ABC cells) - alterations associated with autoimmune diseases. These results suggest that dysfunctional B cells may be part of a mechanism explaining why loss-of-function Hem-1 variants result in recurring infections and autoimmunity.
    MeSH term(s) Actins ; Adaptor Proteins, Signal Transducing/immunology ; Animals ; Autoantibodies ; Autoimmune Diseases ; B-Lymphocytes/immunology ; Immunity, Humoral ; Mice ; Mice, Knockout
    Chemical Substances Actins ; Adaptor Proteins, Signal Transducing ; Autoantibodies ; Hem1 protein, mouse
    Language English
    Publishing date 2022-05-09
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ISSN 2379-3708
    ISSN (online) 2379-3708
    DOI 10.1172/jci.insight.153597
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Triptonide Effectively Inhibits Wnt/β-Catenin Signaling via C-terminal Transactivation Domain of β-catenin.

    Chinison, Jessica / Aguilar, Jose S / Avalos, Alan / Huang, Ying / Wang, Zhijun / Cameron, D Joshua / Hao, Jijun

    Scientific reports

    2016  Volume 6, Page(s) 32779

    Abstract: Abnormal activation of canonical Wnt/β-catenin signaling is implicated in many diseases including cancer. As a result, therapeutic agents that disrupt this signaling pathway have been highly sought after. Triptonide is a key bioactive small molecule ... ...

    Abstract Abnormal activation of canonical Wnt/β-catenin signaling is implicated in many diseases including cancer. As a result, therapeutic agents that disrupt this signaling pathway have been highly sought after. Triptonide is a key bioactive small molecule identified in a traditional Chinese medicine named Tripterygium wilfordii Hook F., and it has a broad spectrum of biological functions. Here we show that triptonide can effectively inhibit canonical Wnt/β-catenin signaling by targeting the downstream C-terminal transcription domain of β-catenin or a nuclear component associated with β-catenin. In addition, triptonide treatment robustly rescued the zebrafish "eyeless" phenotype induced by GSK-3β antagonist 6-bromoindirubin-30-oxime (BIO) for Wnt signaling activation during embryonic gastrulation. Finally, triptonide effectively induced apoptosis of Wnt-dependent cancer cells, supporting the therapeutic potential of triptonide.
    MeSH term(s) Animals ; Cell Nucleus/metabolism ; HEK293 Cells ; Humans ; Protein Transport ; Signal Transduction/drug effects ; Transcriptional Activation ; Triterpenes/pharmacology ; Wnt Proteins/metabolism ; Zebrafish ; beta Catenin/metabolism
    Chemical Substances CTNNB1 protein, human ; Triterpenes ; Wnt Proteins ; beta Catenin ; triptonide (38647-11-9)
    Language English
    Publishing date 2016-09-06
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/srep32779
    Database MEDical Literature Analysis and Retrieval System OnLINE

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