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  1. Article ; Online: Understanding Hepatitis E Viruses by exploring the structural and functional properties of ORF4

    Zoya Shafat / Ayesha Tazeen / Murshad Ahmed

    Network Biology, Vol 11, Iss 4, Pp 277-

    2021  Volume 294

    Abstract: Hepatitis E virus (HEV) belongs to the family Hepeviridae and is the major cause of hepatitis E infections across the globe. Recently, a novel viral protein of HEV, named as open reading frame (ORF4), has been associated with its replication in genotype ... ...

    Abstract Hepatitis E virus (HEV) belongs to the family Hepeviridae and is the major cause of hepatitis E infections across the globe. Recently, a novel viral protein of HEV, named as open reading frame (ORF4), has been associated with its replication in genotype 1 isolates. However, much information regarding ORF4 has not been explored. Thus, the study was conceptualized to explore the structural and functional features of HEV ORF4 protein to better understand the possible molecular mechanisms. The detailed investigation of the ORF4 was carried out in terms of its physicochemical properties, secondary and tertiary structure predictions and functional analysis using different bioinformatics tools. The in silico analyses revealed that ORF4 sequences were enriched in Serine, Proline and Glycine amino acid residues suggesting the prevalence of disordered residues. The protein was found to be thermostable, unstable and highly hydrophobic. The structural analysis showed the presence of cleft, tunnel and pore suggesting their participation in interaction with other molecules. Moreover, identification of several modified sites in ORF4 sequences such as glycosylation, phosphorylation and myristoylation sequences suggest their involvement in cellular signaling pathways and biological processes. Thus, taken together, it can be interpreted that HEV ORF4 possesses significant enormous flexibility due to the presence of Serine, Glycine and Proline amino acids, which suggest its involvement in protein-protein interaction. Furthermore, the presence of motifs, clefts and tunnels also strengthens our analysis, suggesting the commitment of ORF4 towards interaction with other target molecules. Thus, it could be potent drug-targets.
    Keywords hepatitis e virus ; open reading frame (orf4) ; physicochemical parameters ; structural analysis ; functional analysis ; Biology (General) ; QH301-705.5
    Subject code 612
    Language English
    Publishing date 2021-12-01T00:00:00Z
    Publisher International Academy of Ecology and Environmental Sciences
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Circulation of dengue virus serotypes in hyperendemic region of New Delhi, India during 2011–2017

    Arshi Islam / Mohd. Abdullah / Ayesha Tazeen / Irshad H. Naqvi / Syed Naqui Kazim / Anwar Ahmed / Salman Freeh Alamery / Ajamaluddin Malik / Shama Parveen

    Journal of Infection and Public Health, Vol 13, Iss 12, Pp 1912-

    2020  Volume 1919

    Abstract: Background: Dengue fever has become a hampering menace in New Delhi India, since the disease has become hyperendemic, due to circulation of multiple serotypes of dengue virus (DENV). This hyperendemicity poses a greater risk of secondary infections in ... ...

    Abstract Background: Dengue fever has become a hampering menace in New Delhi India, since the disease has become hyperendemic, due to circulation of multiple serotypes of dengue virus (DENV). This hyperendemicity poses a greater risk of secondary infections in human health system. This is a major issue which leads to apprehension amongst the researchers and health organizations and thus requires regular epidemiological surveillance. Methods: We analyzed the prevalence and serotypic distribution of dengue fever cases reported from the Southern part of New Delhi during continued surveillance from 2011 to 2017. The blood samples for the investigation were obtained from the patients suspected with dengue fever attending the OPD at a local Health Centre. The data for 2011–2016 was already published from our laboratory. The samples collected during 2017 were serotyped and characterized in the present study. Results: A total of 565 samples (59%) were positive for DENV of 958 samples tested by RT-PCR during 7 years (2011–2017). Our study has shown that most infections were caused by DENV-2 during 2011−2015. The data has shown occurrence of all four serotypes of DENV during 2015 and predominance of DENV-3 in 2016 and 2017. Further, predominant combination of DENV-1 and DENV-2 was found in most of the co-infections. To the best of our knowledge this is the first study showing the epidemiological trend of dengue fever in reference to the circulating DENV serotypes and co-infections from a hyperendemic region of New Delhi during 2011–2017. Conclusions: This hyperendemic pattern of DENV and instantaneous shift in circulation of its serotypes is likely pose a greater risk of secondary infections. Inclusion of comprehensive community and hospital surveillance of dengue fever will assist in formulation and implementation of effective control measures.
    Keywords Co-infection ; Dengue virus (DENV) ; Epidemiology ; Hyperendemicity ; Serotypes ; Infectious and parasitic diseases ; RC109-216 ; Public aspects of medicine ; RA1-1270
    Subject code 630
    Language English
    Publishing date 2020-12-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Circulation of single serotype of Dengue Virus (DENV-3) in New Delhi, India during 2016

    Nazish Parveen / Arshi Islam / Ayesha Tazeen / Malik Hisamuddin / Mohd Abdullah / Irshad H. Naqvi / Md I. Faizan / Divya Gulyani / Anwar Ahmed / Shama Parveen

    Journal of Infection and Public Health, Vol 12, Iss 1, Pp 49-

    A change in the epidemiological trend

    2019  Volume 56

    Abstract: Background: Dengue is a rapidly emerging arthropod borne viral infection affecting tropical and sub-tropical regions of the world. Dengue is an acute febrile illness but sometimes causes more fatal complications like dengue haemorrhagic fever (DHF) and ... ...

    Abstract Background: Dengue is a rapidly emerging arthropod borne viral infection affecting tropical and sub-tropical regions of the world. Dengue is an acute febrile illness but sometimes causes more fatal complications like dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS). Delhi, the capital of India has become hyper endemic for dengue virus because all the four serotypes are circulating here. Methods: The present study describes the identification of dengue virus from clinical samples collected from the suspected dengue patients from New Delhi, India during 2016. The CprM region of Dengue virus genome was analyzed for phylogenetic, selection pressure and Shannon entropy analyses. Results: The present study reports circulation of a single serotype (DENV-3) in New Delhi, during 2016. The phylogenetic analysis revealed that Indian subcontinent (genotype III) of DENV-3 was circulating in Delhi during this period. Neutral selection pressure in the analyzed region revealed relatively conserved nature of this part of the Dengue virus genome. Amino acid at 31 was positively selected and had high entropy value suggesting probability of variation at this position. Conclusions: The changing trend in circulation of dengue virus serotypes necessitates the continuous epidemiological surveillance for the dengue outbreaks in this region. Keywords: Dengue virus, Epidemiology, Phylogenetic analysis, Selection pressure, Entropy analysis
    Keywords Infectious and parasitic diseases ; RC109-216 ; Public aspects of medicine ; RA1-1270
    Language English
    Publishing date 2019-01-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Evaluation of In-vitro Anthelminthic Activity of Catharanthus roseus Extract

    Swati Agarwal / Simi Jacob / Nikkita Chettri / Saloni Bisoyi / Ayesha Tazeen / A. B. Vedamurthy / V. Krishna / H. Joy Hoskeri

    International Journal of Pharmaceutical Sciences and Drug Research, Vol 3, Iss 3, Pp 211-

    2011  Volume 213

    Abstract: Helminthes infections are chronic illnesses in human beings and in cattle. Pherithema posthuma a helminthes is commonly known as earth-worms. Although the use of alternate drugs has been as a remedial measure against the resistant strains of helminth ... ...

    Abstract Helminthes infections are chronic illnesses in human beings and in cattle. Pherithema posthuma a helminthes is commonly known as earth-worms. Although the use of alternate drugs has been as a remedial measure against the resistant strains of helminth parasites, and as a means of reducing the cost of controlling helminthic diseases. Catharanthus roseus is a medicinally valuable plant and possess various pharmacological properties. Catharanthus roseus has been traditionally used as an anthelminthic agent. To justify the ethnomedical claims, the anthelminthic property of Catharanthus roseus was evaluated using Pherithema posthuma as an experimental model. Piperazine citrate was used as the standard reference. Among the various concentrations tested, ethanol extract at 200 mg/ml showed efficient paralysis effect (6.67 min) than other treated groups, whereas ethanol extract 250 mg/ml showed significant anthelminthic activity with death time of 46.33 min. Standard drug at 50 mg/ml showed paralysis at 31.33 min and death time was 40.67 min. This investigation revealed that ethanol extract of Catharanthus roseus showed significant anthelminthic activity against Pheretima posthuma. Ethanol extract also proved to be efficient than the standard drug. This investigation supported the ethnomedical claims of Catharanthus roseus as an anthelminthic plant.
    Keywords Apocynaceae ; Catharanthus roseus ; anthelminthic activity ; Pheretima posthuma ; ethanol extract ; Therapeutics. Pharmacology ; RM1-950 ; Medicine ; R
    Subject code 630
    Language English
    Publishing date 2011-07-01T00:00:00Z
    Publisher International Journal of Pharmaceutical Sciences and Drug Research
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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