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  1. Article ; Online: A Small Bite on the Eyelid

    Nermin Şakru / Ayşegül Taylan Özkan / Ömer Benian / Mükerrem Duygu Aksoy

    Türkiye Parazitoloji Dergisi, Vol 43, Iss 1, Pp 53-

    2019  Volume 53

    Keywords Medicine ; R ; Infectious and parasitic diseases ; RC109-216
    Language English
    Publishing date 2019-03-01T00:00:00Z
    Publisher Galenos Yayinevi
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Molecular identification of sulfadoxine-pyrimethamine resistance in malaria infected women who received intermittent preventive treatment in the Democratic Republic of Congo

    Emrah Ruh / Jean Paul Bateko / Turgut Imir / Aysegul Taylan-Ozkan

    Malaria Journal, Vol 17, Iss 1, Pp 1-

    2018  Volume 7

    Abstract: Abstract Background Point mutations in Plasmodium falciparum dihydrofolate reductase (Pfdhfr) and dihydropteroate synthase (Pfdhps) genes which confer resistance to sulfadoxine-pyrimethamine (SP) occur at increasing rates. The present study aimed to ... ...

    Abstract Abstract Background Point mutations in Plasmodium falciparum dihydrofolate reductase (Pfdhfr) and dihydropteroate synthase (Pfdhps) genes which confer resistance to sulfadoxine-pyrimethamine (SP) occur at increasing rates. The present study aimed to identify Pfdhfr and Pfdhps mutations in P. falciparum isolates recovered from women who received two doses of SP during pregnancy in Bandundu, the Democratic Republic of Congo (DRC). Methods A total of 48 women with confirmed P. falciparum infection were enrolled in the study. Finger-prick blood samples that were collected on filter paper at the time of delivery were used for DNA isolation. Pfdhfr and Pfdhps genes were amplified by a nested PCR protocol. DNA sequencing was performed on both strands, and the point mutations were analysed. Results All of the 48 (100.0%) P. falciparum isolates carried at least one polymorphism in both genes. The wild-type haplotypes of Pfdhfr (CNCSI [C50, N51, C59, S108, I164]) and Pfdhps (SAKAA [S436, A437, K540, A581, A613]) were not observed in the study. In Pfdhfr, N51I (85.4%), C59R (60.4%), and S108N (100.0%) polymorphisms were detected. Triple mutation (CIRNI) (mutant amino acids are underlined) was the most prevalent (47.9%) Pfdhfr haplotype. In the study, all P. falciparum isolates (100.0%) harboured the A437G allele in Pfdhps gene. Also, K540E and A581G polymorphisms were observed in one (2.1%) isolate. Single mutant haplotype (SGKAA) was detected in 97.9% of the isolates. Mutant Pfdhfr and Pfdhps allele combinations revealed quintuple (CICNI-SGEGA; 2.1%), quadruple (CIRNI-SGKAA; 47.9%), triple (CICNI-SGKAA; 35.4%, CNRNI-SGKAA; 12.5%), and double (CNCNI-SGKAA; 2.1%) haplotypes. Conclusions In the study, the rate of SGEGA haplotype was low (2.1%). Although K540E and A581G alleles are more common in Eastern Africa, a distinct lineage of SGEGA is also present in the DRC, which is located in Central Africa. This haplotype is associated with decreased efficacy of SP in pregnant women and infants, therefore, it should be carefully ...
    Keywords Plasmodium falciparum ; Sulfadoxine-pyrimethamine ; Drug resistance ; Pfdhfr ; Pfdhps ; Democratic Republic of Congo ; Arctic medicine. Tropical medicine ; RC955-962 ; Infectious and parasitic diseases ; RC109-216
    Subject code 616
    Language English
    Publishing date 2018-01-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
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  3. Article ; Online: Investigation of neglected protists Blastocystis sp. and Dientamoeba fragilis in immunocompetent and immunodeficient diarrheal patients using both conventional and molecular methods.

    Fakhriddin Sarzhanov / Funda Dogruman-Al / Monica Santin / Jenny G Maloney / Ayse Semra Gureser / Djursun Karasartova / Aysegul Taylan-Ozkan

    PLoS Neglected Tropical Diseases, Vol 15, Iss 10, p e

    2021  Volume 0009779

    Abstract: Introduction The clinical significance of Blastocystis sp. and Dientamoeba fragilis in patients with gastrointestinal symptoms is a controversial issue. Since the pathogenicity of these protists has not been fully elucidated, testing for these organisms ... ...

    Abstract Introduction The clinical significance of Blastocystis sp. and Dientamoeba fragilis in patients with gastrointestinal symptoms is a controversial issue. Since the pathogenicity of these protists has not been fully elucidated, testing for these organisms is not routinely pursued by most laboratories and clinicians. Thus, the prevalence of these organisms and the subtypes of Blastocystis sp. in human patients in Turkey are not well characterized. This study aimed to determine the prevalence of Blastocystis sp. and D. fragilis in the diarrheic stool samples of immunodeficient and immunocompetent patients using conventional and molecular methods and to identify Blastocystis sp. subtypes using next generation sequencing. Material and methods Individual stool specimens were collected from 245 immunodeficient and 193 immunocompetent diarrheic patients between March 2017 and December 2019 at the Gazi University Training and Research Hospital in Ankara, Turkey. Samples were screened for Blastocystis sp. and D. fragilis by conventional and molecular methods. Molecular detection of both protists was achieved by separate qPCRs targeting a partial fragment of the SSU rRNA gene. Next generation sequencing was used to identify Blastocystis sp. subtypes. Results The prevalence of Blastocystis sp. and D. fragilis was 16.7% and 11.9%, respectively as measured by qPCR. The prevalence of Blastocystis sp. and D. fragilis was lower in immunodeficient patients (12.7% and 10.6%, respectively) compared to immunocompetent patients (21.8% and 13.5%, respectively). Five Blastocystis sp. subtypes were identified and the following subtype distribution was observed: ST3 54.4% (n = 37), ST2 16.2% (n = 11), ST1 4.4% (n = 3), ST6 2.9% (n = 2), ST4 1.5% (n = 1), ST2/ST3 11.8% (n = 8) and ST1/ST3 8.8% (n = 6). There was no statistically significant difference in the distribution of Blastocystis sp. subtypes between immunocompetent and immunodeficient patients. Conclusion and recommendation Our findings demonstrated that Blastocystis sp. and D. ...
    Keywords Arctic medicine. Tropical medicine ; RC955-962 ; Public aspects of medicine ; RA1-1270
    Subject code 616
    Language English
    Publishing date 2021-10-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
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  4. Article ; Online: Report of a rare case of severe allergic reaction due to nasal myiasis and a brief review of the literature in Turkey

    Ali Kemal Erenler / Ahu Pınar Turan / Özlem Oymak Ay / Ayşegül Taylan Özkan

    SAGE Open Medical Case Reports, Vol

    2019  Volume 7

    Abstract: Myiasis is defined as infestation of a mammal by fly larvae. It may occur on either living tissues (primary myiasis) or dead tissues (secondary myiasis). In this report, we present a patient with myiasis with an extremely rare clinical manifestation and ... ...

    Abstract Myiasis is defined as infestation of a mammal by fly larvae. It may occur on either living tissues (primary myiasis) or dead tissues (secondary myiasis). In this report, we present a patient with myiasis with an extremely rare clinical manifestation and severe allergic reaction, and we review the literature in order to reveal the current status. A 20-year-old female patient was admitted to our emergency department due to rush on face, cough and shortness of breath. The maggot came out of her nose was identified as Oestrus ovis . With a diagnosis of severe allergic reaction due to myiasis, she was treated diphenhidramine, prednisone and inhale albuterol in the emergency department. After treatment and further investigation, she was discharged with full recovery. Myiasis is a rare cause for severe allergic reaction in patients with definite diagnosis. Immediate diagnosis and treatment are milestones in preventing bad outcomes.
    Keywords Medicine (General) ; R5-920
    Subject code 610
    Language English
    Publishing date 2019-04-01T00:00:00Z
    Publisher SAGE Publishing
    Document type Article ; Online
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  5. Article ; Online: Evaluation of Formalin-fixed Paraffin-embedded Tissue Samples Diagnosed by Histopathology as Echinococcus in Çorum

    Yılmaz Baş / Yunus Emre Beyhan / Havva Hande Keser Şahin / Tuğba Özçerezci / Dursun Karasartova / Ayşe Semra Güreser / Güven Güney / Ayşegül Taylan Özkan

    Türkiye Parazitoloji Dergisi, Vol 45, Iss 4, Pp 262-

    2021  Volume 267

    Abstract: Objective:This study aimed to detect the presence of Echinococcus spp. in formalin-fixed paraffin-embedded (FFPG) samples of hydatid cyst cases and to discuss the DNA isolation problems in FFPG samples.Methods:FFPG samples of 47 cases diagnosed with ... ...

    Abstract Objective:This study aimed to detect the presence of Echinococcus spp. in formalin-fixed paraffin-embedded (FFPG) samples of hydatid cyst cases and to discuss the DNA isolation problems in FFPG samples.Methods:FFPG samples of 47 cases diagnosed with hydatid cyst were included in this study. Demographic characteristics of the cases were investigated. Microtome sections were taken from the samples and deparaffinization, DNA extraction, polymerase chain reaction (PCR), and gel agarose electrophoresis procedures were performed.Results:Of the cases, 55.3% were female, whereas 45.7% were male. Average age was 45.47 and 68.1% of the cases were located in the liver, 17.0% in the lung, 12.8% in the abdomen, and 2.1% in the brain. DNA was obtained in only 11 (23.4%) of the FFPG cyst samples and no proliferation was detected in the PCR products of any of the sample.Conclusion:The scolex/germinal membrane’ absence in the FFPG sections, intense inflammatory cell reaction, presence of fibrosis and stromal/parenchymal tissue, DNA damage due to formaldehyde action, long-term archiving, and insufficient amount of DNA obtained were considered as factors preventing DNA replication in PCR.
    Keywords hydatid cyst ; echinococcus ; human ; pcr ; Medicine ; R ; Infectious and parasitic diseases ; RC109-216
    Subject code 630
    Language English
    Publishing date 2021-12-01T00:00:00Z
    Publisher Galenos Yayinevi
    Document type Article ; Online
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  6. Article ; Online: Seroprevalance of leishmaniosis and toxoplasmosis in healthy appearanced street dogs in Eskisehir

    Nihal Doğan / Ayşegül Taylan Özkan / Cahit Babür / Cem Köse

    Türk Hijyen ve Deneysel Biyoloji Dergisi, Vol 71, Iss 1, Pp 27-

    2014  Volume 34

    Abstract: OBJECTIVE[|]Leishmaniosis and toxoplasmosis, zoonotic agent, can cause several symptoms in human and animal and have quite importance for public health. In this study, it is aimed to determine the seroprevalence of canin leishmaniosis (CanL) and ... ...

    Abstract OBJECTIVE[|]Leishmaniosis and toxoplasmosis, zoonotic agent, can cause several symptoms in human and animal and have quite importance for public health. In this study, it is aimed to determine the seroprevalence of canin leishmaniosis (CanL) and toxoplasmosis with comparing sex, age and strain in healthy looking street dogs in Eskisehir Province.[¤]METHODS[|]Between July 2011-November 2012, 185 healthy lookig street dogs were caught in Eskişehir city center by officers of Tepebasi Municipality, Veterinary Directorate, Natural Life Center, Eskisehir because of rabies immunization and sterilization. After general health examination by veterinarian, during operation 5 ml blood samples were taken in appropriate conditions and sera were separated for Indirect Fluorescein Antibody Test (IFAT) for searching seropositivity against Leishmania infantum and Sabin Feldman Dye Test (SFDT) for Toxoplasma gondii. The results were evaluated by SPSS version 17.0 program.[¤]RESULTS[|]Out of 107 of 185 dogs were found as seropositive (1/16 and over) by SFDT for T. gondii; no important differences were obtained statistically according to sex, age groups and dog strains (Each p>0.05). None of the samples determined as seropositive (1/64 or over) by IFAT. Total 35 dogs (19%) were found as weak positive (34 dogs in 1/16 and only one in weak positive 1/64 titer) and there were no clinical symptoms of leishmaniosis on these dogs.[¤]CONCLUSION[|]In healthy looking street dogs in Eskisehir Province, seropositivity of T. gondii and weak seropositivity of CanL were determined. It is recommended to take some measures for regular control and prevention of zoonotic infections in owned or street dogs, which could carry important risk for public health.[¤]
    Keywords Indirect Fluorescein Antibody Test ; Dog ; Leishmania infantum ; Sabin Feldman Dye Test ; Toxoplasma gondii ; Biology (General) ; QH301-705.5 ; Science ; Q ; Public aspects of medicine ; RA1-1270 ; Medicine ; R
    Subject code 630
    Language Turkish
    Publishing date 2014-05-01T00:00:00Z
    Publisher Refik Saydam Hıfzıssıhha Merkezi Başkanlığı
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article: An alternative DNA extraction method for detection of Blastocystis spp. in human fecal samples

    Karasartova, Djursun / Ayse Semra Gureser / Aysegul Taylan-Ozkan / Buse Turegun- Atasoy / Emrah Ruh / Leyla Tasci / Mustafa Kerem Calgin

    Experimental parasitology. 2018 Mar., v. 186

    2018  

    Abstract: Polymerase chain reaction (PCR) is an effective technique for diagnosis of Blastocystis infection. Notably, DNA isolation procedure is extremely critical for the PCR step. In the present study, a recently described extraction procedure, named as the “ ... ...

    Abstract Polymerase chain reaction (PCR) is an effective technique for diagnosis of Blastocystis infection. Notably, DNA isolation procedure is extremely critical for the PCR step. In the present study, a recently described extraction procedure, named as the “sand method” was modified and adapted for isolation of Blastocystis DNA. To evaluate its efficacy, the current method and QIAamp DNA Stool Mini Kit (Qiagen) were applied to fresh human stool samples. Our results indicated that, the mean DNA concentrations obtained by the sand method and the commercial kit were 48 and 55 ng/μl, respectively. Also, no DNA inhibitors were detected in two methods. The sand method was capable of detecting 16 parasites per 50 mg feces. DNA samples extracted by both methods were subjected to PCR. Blastocystis spp. were detected in 11 (31.4%) of 35 samples, and perfect agreement (κ: 1.000) was found between the PCR-sand method and PCR-commercial kit method. The samples that were detected positive by PCR-sand method were successfully sequenced, and Blastocystis subtypes (STs) were identified as ST3, ST2 and ST1. In conclusion, the present study indicates that the sand method provides a simple, rapid and inexpensive procedure for reliable extraction of Blastocystis DNA from stool samples.
    Keywords analytical kits ; Blastocystis ; DNA ; feces ; humans ; parasites ; polymerase chain reaction
    Language English
    Dates of publication 2018-03
    Size p. 36-41.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 391089-1
    ISSN 1090-2449 ; 0014-4894
    ISSN (online) 1090-2449
    ISSN 0014-4894
    DOI 10.1016/j.exppara.2018.01.019
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  8. Article: The role of Treg population in pathogenesis of Crimean Congo hemorrhagic fever

    Gazi, Umut / Derya Yapar / Djursun Karasartova / Ayse Semra Gureser / Ozlem Akdogan / Ozgur Unal / Nurcan Baykam / Aysegul Taylan Ozkan

    Virus research. 2018 May 02, v. 250

    2018  

    Abstract: Crimean-Congo hemorrhagic fever (CCHF) is a severe human infection caused by CCHF virus (CCHFV). Today, although the literature on CCHF pathogenesis is still limited, it is thought to be associated with immunosuppression in the early phase of infection ... ...

    Abstract Crimean-Congo hemorrhagic fever (CCHF) is a severe human infection caused by CCHF virus (CCHFV). Today, although the literature on CCHF pathogenesis is still limited, it is thought to be associated with immunosuppression in the early phase of infection followed by pro-inflammatory immune response that may lead to fatal outcomes. The aim of this study is to investigate the role of regulatory T-cells (Treg cells) in the pathogenesis of CCHFV.Peripheral blood mononuclear cell samples collected from 14 acute CCHF patients with mild disease course and 13 healthy subjects were included in this study. Treg expression and functional levels were analyzed by flow cytometry. Treg cells were identified as CD4+CD25 + CD127dim cells, and their functional levels were compared by measuring their ability to suppress CD69 and CD154 expression by activated T-cells.The flow cytometry analysis revealed that total T-cell and helper T-cell levels did not vary between the two groups. In contrast, CCHF patients displayed higher Treg cell levels but lower Treg suppressive activities when compared with control subjects.This is the first study on the involvement of Treg cells in CCHF pathogenesis. Our results indicate that even though Treg cell levels are elevated during acute phase of CCHF infection, not all generated Treg cells has immunosuppressive capacity, and therefore may not represent ‘true’ Treg cell population. Future studies on the intrinsic mechanisms responsible for the reduced Treg inhibitory activities are required for further enlightening the CCHF pathogenesis, especially in the acute phase of the disease.
    Keywords Crimean-Congo hemorrhagic fever ; T-lymphocytes ; disease course ; flow cytometry ; human diseases ; immune response ; immunosuppression ; pathogenesis ; patients ; viruses
    Language English
    Dates of publication 2018-0502
    Size p. 1-6.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 605780-9
    ISSN 1872-7492 ; 0168-1702
    ISSN (online) 1872-7492
    ISSN 0168-1702
    DOI 10.1016/j.virusres.2018.04.003
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  9. Article ; Online: Evaluation of systemic tissue involvement in mice following intraperitoneal inoculation of Toxoplasma gondii RH Ankara strain

    Emine Şamdancı - Türkmen / Ayşegül Taylan - Özkan / Cahit Babür / Mesut Mungan / Engin Aydın

    Türk Hijyen ve Deneysel Biyoloji Dergisi, Vol 72, Iss 1, Pp 27-

    2015  Volume 36

    Abstract: OBJECTIVE[|]This study aimed to pathologically evaluate systemic tissue involvement in acute toxoplasmosis in mice inoculated with Toxoplasma gondii RH Ankara strain.[¤]METHODS[|]Forty-two Mus musculus albino mice were divided into four groups with the ... ...

    Abstract OBJECTIVE[|]This study aimed to pathologically evaluate systemic tissue involvement in acute toxoplasmosis in mice inoculated with Toxoplasma gondii RH Ankara strain.[¤]METHODS[|]Forty-two Mus musculus albino mice were divided into four groups with the control group receiving no inoculation, the group consisting of mice that were not sacrificed after intraperitoneal inoculation and the groups consisting of mice euthanasia were performed on day runner up or day fourth following intraperitoneal inoculation. A 48-50-hour intraperitoneal passage of T. gondii RH Ankara strain in mice was used for inoculation and each mouse received 5x104 tachyzoites. The mice sacrificed by euthanasia were macroscopically examined and then collected tissue samples fixed in 10% formalin. The preparations set by from the tissues blocked in paraffin were stained with hematoxylin-eosin and histopathological examination was performed.[¤]RESULTS[|]Necrosis in the serosa of the intestine and parenchyma of the liver, spleen, kidneys, pancreases and omentum and many free groups of tachyzoites were observed. Tachyzoites were identified in alveolar capillary lumens in the lungs. No pathological finding was observed in the cerebrum and cerebellum but few tachyzoites. It was observed that tissue necrosis and the density of Toxoplasma gondii tachyzoites were directly proportional to the duration of post inoculation. All infected mice that received no intervention died on day sixth. In this study in mice, it was found that T. gondii RH Ankara strain induced the same pathological findings as the RH strain belonging to the virulent Type I group.[¤]CONCLUSION[|]Mice inoculation can be used for diagnosis and this model may also be used in studies investigating vaccination, drugs and pathogenesis.[¤]
    Keywords Toxoplasma gondii ; pathogenesis ; mice ; Biology (General) ; QH301-705.5 ; Science ; Q ; Public aspects of medicine ; RA1-1270 ; Medicine ; R
    Subject code 630
    Language Turkish
    Publishing date 2015-04-01T00:00:00Z
    Publisher Refik Saydam Hıfzıssıhha Merkezi Başkanlığı
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article: Blastocystosis in post-traumatic splenectomized patients

    Karasartova, Djursun / A. Semra Gureser / Aysegul Taylan-Ozkan / Buse Turegun-Atasoy / Mete Dolapci / Musa Zorlu

    Parasitology international. 2016 Dec., v. 65, no. 6

    2016  

    Abstract: The aim of this study was to determine the prevalence and significance of intestinal protozoa, specially Blastocystis spp., and to perform PCR-based subtype classification for understanding the importance of Blastocystis spp. in the pathogenesis of ... ...

    Abstract The aim of this study was to determine the prevalence and significance of intestinal protozoa, specially Blastocystis spp., and to perform PCR-based subtype classification for understanding the importance of Blastocystis spp. in the pathogenesis of gastrointestinal disorders in post-traumatic splenectomized patients.A total of 60 stool samples were obtained from 30 post-traumatic splenectomized patients and 30 healthy controls. Wet mounts, trichrome and Kinyoun acid-fast stained slides were prepared from the stool specimens. PCR was used for detecting the presence of Giardia spp., Entamoeba spp., Dientamoeba fragilis, Cryptosporidium spp., Blastocystis spp. Genotyping was realized by using Blastocystis hominis STS primers.In both study groups, any helminth eggs and other protozoa except Blastocystis spp. were not detected by microscopy and PCR, and also bacterial cultures were negative. Only stool microscopy was positive for Blastocystis spp. in 30% (9 of 30) of splenectomized patients and in 13% (4/30) of healthy controls. PCR for Blastocystis spp. was positive in 40% (12 of 30), B. hominis genotypes were 20% (6/30): STS1 in 10% (3/30) and STS3 in 10% (3/30) of splenectomized patients. In healthy controls Blastocystis spp. was 13% (4/30) by PCR and genotypes of B. hominis was not detected. The difference between the prevalence of Blastocystis spp. infection in splenectomized patients and control groups was statistically significant (p=0.020). Abdominal pain was the most frequent gastrointestinal symptom (p=0.019) among splenectomized patients positive for Blastocystis spp.In post-traumatic healthy splenectomized patients, Blastocystis spp. were found to be the most prevalent protozoa and may be responsible for the gastrointestinal disorders.
    Keywords Blastocystis hominis ; Cryptosporidium ; digestive system diseases ; eggs ; Entamoeba ; feces ; gastrointestinal system ; genotype ; genotyping ; Giardia ; helminths ; microscopy ; pain ; pathogenesis ; patients ; polymerase chain reaction
    Language English
    Dates of publication 2016-12
    Size p. 802-805.
    Publishing place Elsevier Ireland Ltd
    Document type Article
    ZDB-ID 1363151-2
    ISSN 1383-5769
    ISSN 1383-5769
    DOI 10.1016/j.parint.2015.12.004
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