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Article ; Online: Silicone-Based Lubricant-Infused Slippery Coating Covalently Bound to Aluminum Substrates for Underwater Applications.

Prado, Lucia H / Böhringer, David / Mazare, Anca / Sotelo, Lamborghini / Sarau, George / Christiansen, Silke / Fabry, Ben / Schmuki, Patrik / Virtanen, Sannakaisa / Goldmann, Wolfgang H / Tesler, Alexander B

ACS applied materials & interfaces

2023 Volume 15, Issue 26, Page(s) 31776–31786

Abstract: Wetting of solid surfaces is crucial for biological and industrial processes but is also associated with several harmful phenomena such as biofouling and corrosion that limit the effectiveness of various technologies in aquatic environments. Despite ... ...

Abstract	Wetting of solid surfaces is crucial for biological and industrial processes but is also associated with several harmful phenomena such as biofouling and corrosion that limit the effectiveness of various technologies in aquatic environments. Despite extensive research, these challenges remain critical today. Recently, we have developed a facile UV-grafting technique to covalently attach silicone-based coatings to solid substrates. In this study, the grafting process was evaluated as a function of UV exposure time on aluminum substrates. While short-time exposure to UV light results in the formation of lubricant-infused slippery surfaces (LISS), a flat, nonporous variant of slippery liquid-infused porous surfaces, longer exposure leads to the formation of semi-rigid cross-linked polydimethylsiloxane (PDMS) coatings, both covalently bound to the substrate. These coatings were exposed to aquatic media to evaluate their resistance to corrosion and biofouling. While the UV-grafted cross-linked PDMS coating effectively inhibits aluminum corrosion in aquatic environments and allows organisms to grow on the surface, the LISS coating demonstrates improved corrosion resistance but inhibits biofilm adhesion. The synergy between facile and low-cost fabrication, rapid binding kinetics, eco-friendliness, and nontoxicity of the applied materials to aquatic life combined with excellent wetting-repellent characteristics make this technology applicable for implementation in aquatic environments.
Language	English
Publishing date	2023-06-22
Publishing country	United States
Document type	Journal Article
ISSN	1944-8252
ISSN (online)	1944-8252
DOI	10.1021/acsami.3c04508
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Book ; Online: A sustainable infrastructure concept for improved accessibility, reusability, and archival of research software

Koch, Timo / Gläser, Dennis / Seeland, Anett / Roy, Sarbani / Schulze, Katharina / Weishaupt, Kilian / Boehringer, David / Hermann, Sibylle / Flemisch, Bernd

2023

Abstract: Research software is an integral part of most research today and it is widely accepted that research software artifacts should be accessible and reproducible. However, the sustainable archival of research software artifacts is an ongoing effort. We ... ...

Abstract	Research software is an integral part of most research today and it is widely accepted that research software artifacts should be accessible and reproducible. However, the sustainable archival of research software artifacts is an ongoing effort. We identify research software artifacts as snapshots of the current state of research and an integral part of a sustainable cycle of software development, research, and publication. We develop requirements and recommendations to improve the archival, access, and reuse of research software artifacts based on installable, configurable, extensible research software, and sustainable public open-access infrastructure. The described goal is to enable the reuse and exploration of research software beyond published research results, in parallel with reproducibility efforts, and in line with the FAIR principles for data and software. Research software artifacts can be reused in varying scenarios. To this end, we design a multi-modal representation concept supporting multiple reuse scenarios. We identify types of research software artifacts that can be viewed as different modes of the same software-based research result, for example, installation-free configurable browser-based apps to containerized environments, descriptions in journal publications and software documentation, or source code with installation instructions. We discuss how the sustainability and reuse of research software are enhanced or enabled by a suitable archive infrastructure. Finally, at the example of a pilot project at the University of Stuttgart, Germany -- a collaborative effort between research software developers and infrastructure providers -- we outline practical challenges and experiences
Keywords	Computer Science - Software Engineering ; D.2.13 ; H.0 ; D.2.1
Subject code	020
Publishing date	2023-01-26
Publishing country	us
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Fiber alignment in 3D collagen networks as a biophysical marker for cell contractility.

Böhringer, David / Bauer, Andreas / Moravec, Ivana / Bischof, Lars / Kah, Delf / Mark, Christoph / Grundy, Thomas J / Görlach, Ekkehard / O'Neill, Geraldine M / Budday, Silvia / Strissel, Pamela L / Strick, Reiner / Malandrino, Andrea / Gerum, Richard / Mak, Michael / Rausch, Martin / Fabry, Ben

Matrix biology : journal of the International Society for Matrix Biology

2023 Volume 124, Page(s) 39–48

Abstract: Cells cultured in 3D fibrous biopolymer matrices exert traction forces on their environment that induce deformations and remodeling of the fiber network. By measuring these deformations, the traction forces can be reconstructed if the mechanical ... ...

Abstract	Cells cultured in 3D fibrous biopolymer matrices exert traction forces on their environment that induce deformations and remodeling of the fiber network. By measuring these deformations, the traction forces can be reconstructed if the mechanical properties of the matrix and the force-free matrix configuration are known. These requirements limit the applicability of traction force reconstruction in practice. In this study, we test whether force-induced matrix remodeling can instead be used as a proxy for cellular traction forces. We measure the traction forces of hepatic stellate cells and different glioblastoma cell lines and quantify matrix remodeling by measuring the fiber orientation and fiber density around these cells. In agreement with simulated fiber networks, we demonstrate that changes in local fiber orientation and density are directly related to cell forces. By resolving Rho-kinase (ROCK) inhibitor-induced changes of traction forces, fiber alignment, and fiber density in hepatic stellate cells, we show that the method is suitable for drug screening assays. We conclude that differences in local fiber orientation and density, which are easily measurable, can be used as a qualitative proxy for changes in traction forces. The method is available as an open-source Python package with a graphical user interface.
MeSH term(s)	Extracellular Matrix/metabolism ; Cell Line ; Collagen/metabolism
Chemical Substances	Collagen (9007-34-5)
Language	English
Publishing date	2023-11-14
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1183793-7
ISSN	1569-1802 ; 0945-053X
ISSN (online)	1569-1802
ISSN	0945-053X
DOI	10.1016/j.matbio.2023.11.004
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Article ; Online: Synthetic mucus for an ex vivo phonation setup: Creation, application, and effect on excised porcine larynges.

Peters, Gregor / Jakubaß, Bernhard / Weidenfeller, Katrin / Kniesburges, Stefan / Böhringer, David / Wendler, Olaf / Mueller, Sarina K / Gostian, Antoniu-Oreste / Berry, David A / Döllinger, Michael / Semmler, Marion

The Journal of the Acoustical Society of America

2022 Volume 152, Issue 6, Page(s) 3245

Abstract: Laryngeal mucus hydrates and lubricates the deformable tissue of the vocal folds and acts as a boundary layer with the airflow from the lungs. However, the effects of the mucus' viscoelasticity on phonation remain widely unknown and mucus has not yet ... ...

Abstract	Laryngeal mucus hydrates and lubricates the deformable tissue of the vocal folds and acts as a boundary layer with the airflow from the lungs. However, the effects of the mucus' viscoelasticity on phonation remain widely unknown and mucus has not yet been established in experimental procedures of voice research. In this study, four synthetic mucus samples were created on the basis of xanthan with focus on physiological frequency-dependent viscoelastic properties, which cover viscosities and elasticities over 2 orders of magnitude. An established ex vivo experimental setup was expanded by a reproducible and controllable application method of synthetic mucus. The application method and the suitability of the synthetic mucus samples were successfully verified by fluorescence evidence on the vocal folds even after oscillation experiments. Subsequently, the impact of mucus viscoelasticity on the oscillatory dynamics of the vocal folds, the subglottal pressure, and acoustic signal was investigated with 24 porcine larynges (2304 datasets). Despite the large differences of viscoelasticity, the phonatory characteristics remained stable with only minor statistically significant differences. Overall, this study increased the level of realism in the experimental setup for replication of the phonatory process enabling further research on pathological mucus and exploration of therapeutic options.
MeSH term(s)	Swine ; Animals ; Larynx/physiology ; Vocal Cords/physiology ; Phonation/physiology ; Mucus ; Acoustics
Language	English
Publishing date	2022-12-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	219231-7
ISSN	1520-8524 ; 0001-4966
ISSN (online)	1520-8524
ISSN	0001-4966
DOI	10.1121/10.0015364
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Conference proceedings: Synthetischer Mukus für ein ex-vivo Phonationsexperiment: Herstellung, Anwendung und Validierung in Schweinekehlköpfen

Semmler, Marion / Peters, Gregor / Jakubaß, Bernhard / Kniesburges, Stefan / Böhringer, David / Berry, David / Wendler, Olaf / Döllinger, Michael

2022 , Page(s) V4

Abstract: Hintergrund: Die Phonation wird als Fluid-Struktur-Akustik-Interaktion beschrieben. Die Wechselwirkung zwischen Luftstrom und Stimmlippengewebe wird weitgehend von den Gewebseigenschaften der Stimmlippen bestimmt, die den Fluss durch die Glottis ... ...

Event/congress	38. Wissenschaftliche Jahrestagung der Deutschen Gesellschaft für Phoniatrie und Pädaudiologie (DGPP); Leipzig; Deutsche Gesellschaft für Phoniatrie und Pädaudiologie; 2022
Abstract	Hintergrund: Die Phonation wird als Fluid-Struktur-Akustik-Interaktion beschrieben. Die Wechselwirkung zwischen Luftstrom und Stimmlippengewebe wird weitgehend von den Gewebseigenschaften der Stimmlippen bestimmt, die den Fluss durch die Glottis begrenzen. Die Energieübertragung zwischen Luft und Stimmlippen wird zudem durch den laryngealen Mukus beeinflusst, der die Grenzschicht zwischen den beiden Systemen bildet. Es gibt zahlreiche Studien über die Auswirkungen der Hydratation auf den Phonationsprozess, aber der Einfluss der Zusammensetzung und Rheologie des laryngealen Mukus wurden bisher kaum untersucht. Material und Methoden: Es wurden 4 synthetische Mukusproben mit physiologischen viskoelastischen Eigenschaften hergestellt. Als Grundlage dienten Untersuchungen zu humanen Mukusproben von Patienten ohne Stimmerkrankung, die aufgrund ihrer sich unterscheidenden viskoelastischen Eigenschaften gruppiert wurden. Wir verwendeten Xanthanlösungen in 3 verschiedenen Konzentrationen und fügten einer von ihnen Muzin zu. Die Wirkung von synthetischem Mukus auf die Phonation wurde in einem etablierter Versuchsaufbau für exzidierte Kehlköpfe unter kontrollierbaren Bedingungen untersucht. Der bestehende Aufbau wurde um eine Methode zur reproduzierbaren Applikation des synthetischen Mukus erweitert. Jede der 4 Mukusproben wurde auf 6 exzidierte Schweinekehlköpfe aufgetragen und in Schwingungsexperimenten mit systematischer Parametervariation multimodal analysiert. Ergebnisse: Mit Hilfe der Particle Tracking Microrheology (PTM) konnten wir nachweisen, dass die viskoelastischen Eigenschaften des synthetischen Mukus mit der Rheologie des menschlichen laryngealen Mukus in den essentiellen Punkten übereinstimmen. Trotz der großen rheologischen Bandbreite, erwiesen sich die Phonationsparameter in den Schwingungsexperimenten als sehr robust. Sie lagen im typischen Bereich anderer ex-vivo Experimente. Ein fluoreszierender Farbstoff bestätigt die Benetzung der gesamten Stimmlippenoberfläche vor und nach den Oszillationsexperimenten. Diskussion: Dieses grundlegende Experiment zeigt die Anwendbarkeit von synthetischem Mukus in ex-vivo Oszillationsexperimenten an exzidierten Kehlköpfen. Dies ermöglicht zukünftig eine systematische Analyse der Auswirkungen von physiologischem und auch pathologischem Mukus mit unterschiedlichen Inhaltsstoffen auf den Phonationsprozess. Langfristig hoffen wir, synthetischen Mukus mit therapeutischem Nutzen für die in-vivo Anwendung zu entwickeln.
Keywords	Medizin, Gesundheit
Publishing date	2022-09-26
Publisher	German Medical Science GMS Publishing House; Düsseldorf
Document type	Conference proceedings
DOI	10.3205/22dgpp05
Database	German Medical Science

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Article: Human Laryngeal Mucus from the Vocal Folds: Rheological Characterization by Particle Tracking Microrheology and Oscillatory Shear Rheology.

Peters, Gregor / Wendler, Olaf / Böhringer, David / Gostian, Antoniu-Oreste / Müller, Sarina K / Canziani, Herbert / Hesse, Nicolas / Semmler, Marion / Berry, David A / Kniesburges, Stefan / Peukert, Wolfgang / Döllinger, Michael

Applied sciences (Basel, Switzerland)

2021 Volume 11, Issue 7

Abstract: Mucus consistency affects voice physiology and is connected to voice disorders. Nevertheless, the rheological characteristics of human laryngeal mucus from the vocal folds remain unknown. Knowledge about mucus viscoelasticity enables fabrication of ... ...

Abstract	Mucus consistency affects voice physiology and is connected to voice disorders. Nevertheless, the rheological characteristics of human laryngeal mucus from the vocal folds remain unknown. Knowledge about mucus viscoelasticity enables fabrication of artificial mucus with natural properties, more realistic ex-vivo experiments and promotes a better understanding and improved treatment of dysphonia with regard to mucus consistency. We studied human laryngeal mucus samples from the vocal folds with two complementary approaches: 19 samples were successfully applied to particle tracking microrheology (PTM) and five additional samples to oscillatory shear rheology (OSR). Mucus was collected by experienced laryngologists from patients together with demographic data. The analysis of the viscoelasticity revealed diversity among the investigated mucus samples according to their rigidity (absolute G' and G″). Moreover some samples revealed throughout solid-like character (G' > G″), whereas some underwent a change from solid-like to liquid-like (G' < G″). This led to a subdivision into three groups. We assume that the reason for the differences is a variation in the hydration level of the mucus, which affects the mucin concentration and network formation factors of the mucin mesh. The demographic data could not be correlated to the differences, except for the smoking behavior. Mucus of predominant liquid-like character was associated with current smokers.
Language	English
Publishing date	2021-03-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704225-X
ISSN	2076-3417
ISSN	2076-3417
DOI	10.3390/app11073011
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Article ; Online: Measurement of Skeletal Muscle Fiber Contractility with High-Speed Traction Microscopy.

Rausch, Martin / Böhringer, David / Steinmann, Martin / Schubert, Dirk W / Schrüfer, Stefan / Mark, Christoph / Fabry, Ben

Biophysical journal

2019 Volume 118, Issue 3, Page(s) 657–666

Abstract: We describe a technique for simultaneous quantification of the contractile forces and cytosolic calcium dynamics of muscle fibers embedded in three-dimensional biopolymer gels under auxotonic loading conditions. We derive a scaling law for linear elastic ...

Abstract	We describe a technique for simultaneous quantification of the contractile forces and cytosolic calcium dynamics of muscle fibers embedded in three-dimensional biopolymer gels under auxotonic loading conditions. We derive a scaling law for linear elastic matrices such as basement membrane extract hydrogels (Matrigel) that allows us to measure contractile force from the shape of the relaxed and contracted muscle cell and the Young's modulus of the matrix without further knowledge of the matrix deformations surrounding the cell and without performing computationally intensive inverse force reconstruction algorithms. We apply our method to isolated mouse flexor digitorum brevis (FDB) fibers that are embedded in 10 mg/mL Matrigel. Upon electrical stimulation, individual FDB fibers show twitch forces of 0.37 ± 0.15 μN and tetanic forces (100-Hz stimulation frequency) of 2.38 ± 0.71 μN, corresponding to a tension of 0.44 ± 0.25 kPa and 2.53 ± 1.17 kPa, respectively. Contractile forces of FDB fibers increase in response to caffeine and the troponin-calcium stabilizer tirasemtiv, similar to responses measured in whole muscle. From simultaneous high-speed measurements of cell length changes and cytosolic calcium concentration using confocal line scanning at a frequency of 2048 Hz, we show that twitch and tetanic force responses to electric pulses follow the low-pass filtered calcium signal. In summary, we present a technically simple high-speed method for measuring contractile forces and cytosolic calcium dynamics of single muscle fibers. We expect that our method will help to reduce preparation time, costs, and the number of sacrificed animals needed for experiments such as drug testing.
MeSH term(s)	Animals ; Calcium ; Electric Stimulation ; Mice ; Microscopy ; Muscle Contraction ; Muscle Fibers, Skeletal ; Muscle, Skeletal ; Traction
Chemical Substances	Calcium (SY7Q814VUP)
Language	English
Publishing date	2019-12-24
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	218078-9
ISSN	1542-0086 ; 0006-3495
ISSN (online)	1542-0086
ISSN	0006-3495
DOI	10.1016/j.bpj.2019.12.014
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Zs.A 235: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 2: Show issues

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Article ; Online: Long-term stability of aerophilic metallic surfaces underwater.

Nature materials

2023 Volume 22, Issue 12, Page(s) 1548–1555

Abstract: Aerophilic surfaces immersed underwater trap films of air known as plastrons. Plastrons have typically been considered impractical for underwater engineering applications due to their metastable performance. Here, we describe aerophilic titanium alloy ( ... ...

Abstract	Aerophilic surfaces immersed underwater trap films of air known as plastrons. Plastrons have typically been considered impractical for underwater engineering applications due to their metastable performance. Here, we describe aerophilic titanium alloy (Ti) surfaces with extended plastron lifetimes that are conserved for months underwater. Long-term stability is achieved by the formation of highly rough hierarchically structured surfaces via electrochemical anodization combined with a low-surface-energy coating produced by a fluorinated surfactant. Aerophilic Ti surfaces drastically reduce blood adhesion and, when submerged in water, prevent adhesion of bacteria and marine organisms such as barnacles and mussels. Overall, we demonstrate a general strategy to achieve the long-term stability of plastrons on aerophilic surfaces for previously unattainable underwater applications.
Language	English
Publishing date	2023-09-18
Publishing country	England
Document type	Journal Article
ZDB-ID	2088679-2
ISSN	1476-4660 ; 1476-1122
ISSN (online)	1476-4660
ISSN	1476-1122
DOI	10.1038/s41563-023-01670-6
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Book ; Thesis: Heroenkulte in Griechenland von der geometrischen bis zur klassischen Zeit

Boehringer, David

Attika, Argolis, Messenien

(Klio : Beihefte ; N.F., 3)

2001

Author's details	David Boehringer
Series title	Klio : Beihefte ; N.F., 3
Language	German ; Greek
Size	398 S, Kt
Publisher	Akad.-Verl
Publishing place	Berlin
Document type	Book ; Thesis
Thesis / German Habilitation thesis	Univ., Diss.--Freiburg, 1998
Note	Teilw. in griech. Schr. ; Literaturverz. S. [377] - 390
ISBN	3050036435 ; 9783050036434
Database	Former special subject collection: coastal and deep sea fishing

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Book ; Thesis: Heroenkulte in Griechenland von der geometrischen bis zur klassischen Zeit

Boehringer, David

Attika, Argolis, Messenien

(Klio : Beihefte ; N.F., 3)

2001

Author's details	David Boehringer
Series title	Klio : Beihefte ; N.F., 3
Language	German
Size	398 S, Kt
Publisher	Akad.-Verl
Publishing place	Berlin
Document type	Book ; Thesis
Thesis / German Habilitation thesis	Zugl.: Freiburg, Univ., Diss., 1998
Note	Teilw. in griech. Schr. ; Literaturverz. S. [377] - 390
ISBN	3050036435 ; 9783050036434
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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