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Article ; Online: Antidiabetic Action of Mcy Protein: Studies on Gene Expression and Competitive Binding to Insulin Receptors.

Marella, Saritha / Kotha, Peddanna / Nabi, S Abdul / Girish, B P / Badri, Kameswara Rao / Chippada, Apparao

2022 Volume 194, Issue 8, Page(s) 3541–3557

Abstract: Mcy protein, isolated from the fruits of Momordica cymbalaria, was shown to have antihyperglycemic, antihyperlipidemic activities along with renal as well as hepatoprotective activities in streptozotocin-induced diabetic rats. Mcy protein was shown to ... ...

Abstract	Mcy protein, isolated from the fruits of Momordica cymbalaria, was shown to have antihyperglycemic, antihyperlipidemic activities along with renal as well as hepatoprotective activities in streptozotocin-induced diabetic rats. Mcy protein was shown to have insulin-like structure and/or function and/or insulin secretagogue activity. Hence, the present study was conducted to elucidate the molecular mechanism whereby Mcy protein elicits its therapeutic role and also to know whether the Mcy protein has any structural and functional similarity with insulin. Results of our experiments revealed that the Mcy protein is insulin-like protein. Furthermore, we assessed the effect of treatment with Mcy protein on the glucose transport (levels of glucose transporter, GLUT-2) and on the levels of key regulators of glucose and lipid metabolisms like hepatic glucokinase (GK) and sterol regulatory element-binding protein-1c (SREBP-1c). Our findings demonstrated that Mcy protein elevated the expressions of GK, SREBP-1c, and GLUT-2 that were decreased in diabetic animals. Insulin-receptor binding studies using rat erythrocytes demonstrated that mean specific binding of insulin with insulin receptors was significantly increased in Mcy-treated diabetic rats when compared to diabetic control rats. Scatchard analyses of insulin binding studies yielded curvilinear plots, and the number of receptor sites per cell was found to be 180 ± 21.1 in Mcy-treated diabetic animals and found to be significantly superior to those of diabetic control animals. Kinetic analyses also revealed an increase in the average receptor affinity of erythrocytes of Mcy-treated rats compared to diabetic control rats suggesting acute alteration in the number and affinity of insulin receptors on the membranes of erythrocytes.
MeSH term(s)	Animals ; Binding, Competitive ; Diabetes Mellitus, Experimental/drug therapy ; Diabetes Mellitus, Experimental/genetics ; Diabetes Mellitus, Experimental/metabolism ; Gene Expression ; Glucokinase/genetics ; Glucokinase/metabolism ; Glucose/metabolism ; Hypoglycemic Agents/pharmacology ; Insulin ; Liver/metabolism ; Plant Proteins/pharmacology ; Rats ; Receptor, Insulin/metabolism ; Sterol Regulatory Element Binding Protein 1/genetics ; Sterol Regulatory Element Binding Protein 1/metabolism ; Sterol Regulatory Element Binding Protein 1/pharmacology
Chemical Substances	Hypoglycemic Agents ; Insulin ; Plant Proteins ; Srebf1 protein, rat ; Sterol Regulatory Element Binding Protein 1 ; Glucokinase (EC 2.7.1.2) ; Receptor, Insulin (EC 2.7.10.1) ; Glucose (IY9XDZ35W2)
Language	English
Publishing date	2022-04-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	392344-7
ISSN	1559-0291 ; 0273-2289
ISSN (online)	1559-0291
ISSN	0273-2289
DOI	10.1007/s12010-022-03824-9
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Article ; Online: Targeting cytokine storm as the potential anti-viral therapy: Implications in regulating SARS-CoV-2 pathogenicity.

Maity, Subhasish / Santra, Ayantika / Hebbani, Ananda Vardhan / Pulakuntla, Swetha / Chatterjee, Ankita / Badri, Kameswara Rao / Damodara Reddy, Vaddi

Gene

2023 Volume 881, Page(s) 147612

Abstract: The latest global pandemic corona virus disease - 2019 (COVID-19) caused by the virus SARS-CoV-2 is still a matter of worrying concern both for the scientific communities and health care organizations. COVID-19 disease is proved to be a highly contagious ...

Abstract	The latest global pandemic corona virus disease - 2019 (COVID-19) caused by the virus SARS-CoV-2 is still a matter of worrying concern both for the scientific communities and health care organizations. COVID-19 disease is proved to be a highly contagious disease transmitted through respiratory droplets and even close contact with affected individuals. COVID-19 disease is also understood to exhibit diverse symptoms of ranging severities i.e., from mild fatigue to death. Affected individuals' susceptibility to induce immunologic dysregulation phenomena termed 'cytokine storm' seems to be playing the damaging role of escalating the disease manifestation from mild to severe. Cytokine storm in patients with severe symptoms is understood to be characterized by enhanced serum levels of many cytokines including interleukin-1β, interleukin-6, IP-10/CXCL10, TNF, interferon-γ, MIP-1α, MIP-1β and VEGF. Since cytokine production in general is the most important antiviral defense response, understanding the COVID-19 associated cytokine storm in particular and differentiating it from the regular cytokine production response becomes crucial in developing an effective therapeutic strategy.This review focuses on the potential targeting of COVID-19 associated cytokine storm and its challenges.
MeSH term(s)	Humans ; SARS-CoV-2 ; COVID-19 ; Virulence ; Cytokines ; Antiviral Agents/therapeutic use
Chemical Substances	Cytokines ; Antiviral Agents
Language	English
Publishing date	2023-07-08
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	391792-7
ISSN	1879-0038 ; 0378-1119
ISSN (online)	1879-0038
ISSN	0378-1119
DOI	10.1016/j.gene.2023.147612
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Article ; Online: COVID-19: comprehensive review on mutations and current vaccines

Hebbani, Ananda Vardhan / Pulakuntla, Swetha / Pannuru, Padmavathi / Aramgam, Sreelatha / Badri, Kameswara Rao / Reddy, Vaddi Damodara

Arch Microbiol. 2022 Jan., v. 204, no. 1 p.8-8

2022

Abstract: Viral outbreaks had been a threat for the human race for a long time. Several epidemics and pandemics have been reported in the past with serious consequences on human health and subsequent social and economic aspects. According to WHO, viral infections ... ...

Abstract	Viral outbreaks had been a threat for the human race for a long time. Several epidemics and pandemics have been reported in the past with serious consequences on human health and subsequent social and economic aspects. According to WHO, viral infections continue to be a major health concern globally. Novel coronavirus, SARS-CoV-2 (Severe acute respiratory syndrome coronavirus-2) causes the most recent infectious pandemic disease, COVID-19 (Coronavirus disease-19). As of now, there were 249 million infections of COVID-19 worldwide with a high mortality of more than 5 million deaths reported; and the number of new additional cases is drastically increasing. Development of therapies to treat the infected cases and prophylactic agents including vaccines that are effective towards different variants are crucial to curtail the COVID-19 pandemic. Owing to the fact that there is a high mortality and morbidity rate along with the risk of virus causing further epidemic outbursts, development of additional effective therapeutic and preventive strategies are highly warranted. Prevention, early detection and treatment will reduce the spread of COVID-19 pandemic. The present review highlights the novel mutations and therapeutic updates associated with coronaviruses along with the clinical manifestations—diagnosis, clinical management and, prophylactic and therapeutic strategies of COVID-19 infection.
Keywords	COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; human health ; humans ; morbidity ; mortality ; pandemic ; risk ; therapeutics ; viruses
Language	English
Dates of publication	2022-01
Size	p. 8.
Publishing place	Springer Berlin Heidelberg
Document type	Article ; Online
Note	Review
ZDB-ID	124824-8
ISSN	1432-072X ; 0302-8933
ISSN (online)	1432-072X
ISSN	0302-8933
DOI	10.1007/s00203-021-02606-x
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Article ; Online: Antidiabetic Action of Mcy Protein: Studies on Gene Expression and Competitive Binding to Insulin Receptors

Marella, Saritha / Kotha, Peddanna / Nabi, S. Abdul / Girish, B. P. / Badri, Kameswara Rao / Chippada, Apparao

Appl Biochem Biotechnol. 2022 Aug., v. 194, no. 8 p.3541-3557

2022

Abstract	Mcy protein, isolated from the fruits of Momordica cymbalaria, was shown to have antihyperglycemic, antihyperlipidemic activities along with renal as well as hepatoprotective activities in streptozotocin-induced diabetic rats. Mcy protein was shown to have insulin-like structure and/or function and/or insulin secretagogue activity. Hence, the present study was conducted to elucidate the molecular mechanism whereby Mcy protein elicits its therapeutic role and also to know whether the Mcy protein has any structural and functional similarity with insulin. Results of our experiments revealed that the Mcy protein is insulin-like protein. Furthermore, we assessed the effect of treatment with Mcy protein on the glucose transport (levels of glucose transporter, GLUT-2) and on the levels of key regulators of glucose and lipid metabolisms like hepatic glucokinase (GK) and sterol regulatory element-binding protein-1c (SREBP-1c). Our findings demonstrated that Mcy protein elevated the expressions of GK, SREBP-1c, and GLUT-2 that were decreased in diabetic animals. Insulin-receptor binding studies using rat erythrocytes demonstrated that mean specific binding of insulin with insulin receptors was significantly increased in Mcy-treated diabetic rats when compared to diabetic control rats. Scatchard analyses of insulin binding studies yielded curvilinear plots, and the number of receptor sites per cell was found to be 180 ± 21.1 in Mcy-treated diabetic animals and found to be significantly superior to those of diabetic control animals. Kinetic analyses also revealed an increase in the average receptor affinity of erythrocytes of Mcy-treated rats compared to diabetic control rats suggesting acute alteration in the number and affinity of insulin receptors on the membranes of erythrocytes.
Keywords	Momordica cymbalaria ; erythrocytes ; gene expression ; glucokinase ; glucose ; glucose transporters ; glycemic effect ; insulin ; insulin receptors ; rats ; sterols ; therapeutics
Language	English
Dates of publication	2022-08
Size	p. 3541-3557.
Publishing place	Springer US
Document type	Article ; Online
ZDB-ID	392344-7
ISSN	0273-2289
ISSN	0273-2289
DOI	10.1007/s12010-022-03824-9
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Article ; Online: COVID-19: comprehensive review on mutations and current vaccines.

Hebbani, Ananda Vardhan / Pulakuntla, Swetha / Pannuru, Padmavathi / Aramgam, Sreelatha / Badri, Kameswara Rao / Reddy, Vaddi Damodara

Archives of microbiology

2021 Volume 204, Issue 1, Page(s) 8

Abstract	Viral outbreaks had been a threat for the human race for a long time. Several epidemics and pandemics have been reported in the past with serious consequences on human health and subsequent social and economic aspects. According to WHO, viral infections continue to be a major health concern globally. Novel coronavirus, SARS-CoV-2 (Severe acute respiratory syndrome coronavirus-2) causes the most recent infectious pandemic disease, COVID-19 (Coronavirus disease-19). As of now, there were 249 million infections of COVID-19 worldwide with a high mortality of more than 5 million deaths reported; and the number of new additional cases is drastically increasing. Development of therapies to treat the infected cases and prophylactic agents including vaccines that are effective towards different variants are crucial to curtail the COVID-19 pandemic. Owing to the fact that there is a high mortality and morbidity rate along with the risk of virus causing further epidemic outbursts, development of additional effective therapeutic and preventive strategies are highly warranted. Prevention, early detection and treatment will reduce the spread of COVID-19 pandemic. The present review highlights the novel mutations and therapeutic updates associated with coronaviruses along with the clinical manifestations-diagnosis, clinical management and, prophylactic and therapeutic strategies of COVID-19 infection.
MeSH term(s)	COVID-19 ; Humans ; Mutation ; Pandemics ; SARS-CoV-2 ; Vaccines
Chemical Substances	Vaccines
Language	English
Publishing date	2021-12-06
Publishing country	Germany
Document type	Journal Article ; Review
ZDB-ID	124824-8
ISSN	1432-072X ; 0302-8933
ISSN (online)	1432-072X
ISSN	0302-8933
DOI	10.1007/s00203-021-02606-x
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Article: P311, a novel intrinsically disordered protein, regulates adipocyte development

Nunez, Sha’Kayla / Young, Corey / Adebayo, Olayinka / Muppuru, Kesavulu Muni / Badri, Kameswara Rao

Biochemical and biophysical research communications. 2019 July 12, v. 515, no. 1

2019

Abstract: Adipocyte development and adipose tissue expansion have many implications for human diseases, including obesity. Obesity is a debilitating disorder and a risk factor for metabolic disorders including insulin resistance and diabetes mellitus, due in part ... ...

Abstract	Adipocyte development and adipose tissue expansion have many implications for human diseases, including obesity. Obesity is a debilitating disorder and a risk factor for metabolic disorders including insulin resistance and diabetes mellitus, due in part to an overabundance of adipocytes and adipocyte dysfunction. In recent years, obesity has become a global pandemic with approximately one-third of US adults classified as obese. Adipose tissue has recently been identified as a major metabolic organ, classified into white adipose tissue (WAT) and brown adipose tissue (BAT). Other than lifestyle modifications and invasive surgeries, only a very limited number of drugs are available to treat obesity and overweight.P311 has been shown to play a key role in blood pressure regulation, vascular contractility and tissue remodeling. Here we present a role for P311 in adipogenesis using a 3T3-L1 cell culture model. P311 expression is initiated with the induction of adipogenesis and increased during adipogenesis. This increase correlates with an increase in the expression of the key adipogenic transcriptional factors PPARγ2 and C/EBPα. In addition, siRNA-mediated P311 knockdown inhibits adipogenic differentiation in 3T3-L1 cells. Finally, P311 binds to the PPARγ2 promoter, implicating P311 mediates adipogenesis partly through PPARγ activation.
Keywords	adipocytes ; adipogenesis ; adults ; blood pressure ; brown adipose tissue ; cell culture ; diabetes mellitus ; drugs ; human diseases ; insulin resistance ; lifestyle ; models ; obesity ; pandemic ; peroxisome proliferator-activated receptor gamma ; risk factors ; surgery ; white adipose tissue ; United States
Language	English
Dates of publication	2019-0712
Size	p. 234-240.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	205723-2
ISSN	0006-291X ; 0006-291X
ISSN (online)	0006-291X
ISSN	0006-291X
DOI	10.1016/j.bbrc.2019.05.105
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Article: Anti-Obesity and Lipid Lowering Activity of Bauhiniastatin-1 is Mediated Through PPAR-γ/AMPK Expressions in Diet-Induced Obese Rat Model.

Karunakaran, Reddy Sankaran / Lokanatha, Oruganti / Muni Swamy, Ganjayi / Venkataramaiah, Chintha / Muni Kesavulu, Muppuru / Appa Rao, Chippada / Badri, Kameswara Rao / Balaji, Meriga

Frontiers in pharmacology

2021 Volume 12, Page(s) 704074

Abstract: Objective: ...

Abstract	Objective:
Language	English
Publishing date	2021-07-22
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2021.704074
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Article ; Online: P311, a novel intrinsically disordered protein, regulates adipocyte development.

Nunez, Sha'Kayla / Young, Corey / Adebayo, Olayinka / Muppuru, Kesavulu Muni / Badri, Kameswara Rao

Biochemical and biophysical research communications

2019 Volume 515, Issue 1, Page(s) 234–240

Abstract	Adipocyte development and adipose tissue expansion have many implications for human diseases, including obesity. Obesity is a debilitating disorder and a risk factor for metabolic disorders including insulin resistance and diabetes mellitus, due in part to an overabundance of adipocytes and adipocyte dysfunction. In recent years, obesity has become a global pandemic with approximately one-third of US adults classified as obese. Adipose tissue has recently been identified as a major metabolic organ, classified into white adipose tissue (WAT) and brown adipose tissue (BAT). Other than lifestyle modifications and invasive surgeries, only a very limited number of drugs are available to treat obesity and overweight. P311 has been shown to play a key role in blood pressure regulation, vascular contractility and tissue remodeling. Here we present a role for P311 in adipogenesis using a 3T3-L1 cell culture model. P311 expression is initiated with the induction of adipogenesis and increased during adipogenesis. This increase correlates with an increase in the expression of the key adipogenic transcriptional factors PPARγ2 and C/EBPα. In addition, siRNA-mediated P311 knockdown inhibits adipogenic differentiation in 3T3-L1 cells. Finally, P311 binds to the PPARγ2 promoter, implicating P311 mediates adipogenesis partly through PPARγ activation.
MeSH term(s)	3T3-L1 Cells ; Adipocytes/cytology ; Adipocytes/metabolism ; Adipogenesis/genetics ; Adipose Tissue, Brown/cytology ; Adipose Tissue, Brown/metabolism ; Adipose Tissue, White/cytology ; Adipose Tissue, White/metabolism ; Animals ; CCAAT-Enhancer-Binding Protein-alpha/genetics ; CCAAT-Enhancer-Binding Protein-alpha/metabolism ; Cell Differentiation/genetics ; Gene Expression Profiling/methods ; Intrinsically Disordered Proteins/genetics ; Intrinsically Disordered Proteins/metabolism ; Mice ; Nerve Tissue Proteins/genetics ; Nerve Tissue Proteins/metabolism ; Obesity/genetics ; PPAR gamma/genetics ; PPAR gamma/metabolism
Chemical Substances	CCAAT-Enhancer-Binding Protein-alpha ; Intrinsically Disordered Proteins ; Nerve Tissue Proteins ; P311 protein, mouse ; PPAR gamma
Language	English
Publishing date	2019-05-27
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	205723-2
ISSN	1090-2104 ; 0006-291X ; 0006-291X
ISSN (online)	1090-2104 ; 0006-291X
ISSN	0006-291X
DOI	10.1016/j.bbrc.2019.05.105
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Article ; Online: Evaluation of biochemical mechanisms of anti-diabetic functions of Anisomeles malabarica.

Kotha, Peddanna / Marella, Saritha / Allagadda, Rajasekhar / Badri, Kameswara Rao / Chippada, Appa Rao

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

2019 Volume 112, Page(s) 108598

Abstract: Anisomeles malabarica (AM) is an aromatic plant traditionally used for the treatment of diabetes mellitus in India. Following bioassay guided fractionation, we recently identified an active fraction of AM (AMAF, with potential mix of active principles) ... ...

Abstract	Anisomeles malabarica (AM) is an aromatic plant traditionally used for the treatment of diabetes mellitus in India. Following bioassay guided fractionation, we recently identified an active fraction of AM (AMAF, with potential mix of active principles) that showed significant antihyperglycemic and antihyperlipidemic activities. In addition, AMAF treatment improved insulin levels. However, the biochemical mechanism/s through which AMAF demonstrates the antidiabetic effects is largely unknown. Based on its beneficial effects we investigated the biochemical mechanism of the anti-diabetic activity of A.malabarica active fraction (AMAF) in streptozotocin (STZ) induced diabetic rats. Streptozotocin induced diabetic rats were treated with AMAF (50 mg AMAF/kg/day) for 30 days and alterations in the body weights, glycogen and protein content of tissues, functional markers of hepatic and renal tissues, carbohydrate metabolic enzymes and their genes expression were evaluated. Lipid peroxides levels and activities of antioxidant enzymes of hepatic and renal tissues were also measured. The AMAF treatment resulted in increase in body weights, hepatic and renal protein and tissue glycogen levels in diabetic treated rats compared to diabetic rats. In addition, the treatment improved activities of carbohydrate metabolic enzymes, antioxidant enzymes and, liver and renal functional markers in the AMAF treated diabetic rats. Gene expressions of key carbohydrate metabolic enzymes/factors glucokinase, glucose transporter protein (GLUT-2) and phosphoenolpyruvate carboxykinase (PEPCK) were also normalized up on AMAF treatment in diabetic rats. Our studies indicate that the isolated active fraction of AM (AMAF) from the leaves of A.malabarica positively regulated the glucose homeostasis and oxidative stress through carbohydrate metabolism and antioxidant enzyme activities respectively in hepatic and renal tissues.
MeSH term(s)	Animals ; Biomechanical Phenomena/drug effects ; Biomechanical Phenomena/physiology ; Blood Glucose/drug effects ; Blood Glucose/metabolism ; Diabetes Mellitus, Experimental/blood ; Diabetes Mellitus, Experimental/chemically induced ; Diabetes Mellitus, Experimental/drug therapy ; Drug Evaluation, Preclinical/methods ; Hypoglycemic Agents/isolation & purification ; Hypoglycemic Agents/pharmacology ; Hypoglycemic Agents/therapeutic use ; Lamiaceae ; Lipid Peroxidation/drug effects ; Lipid Peroxidation/physiology ; Male ; Plant Extracts/isolation & purification ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Rats ; Rats, Wistar ; Streptozocin/toxicity
Chemical Substances	Blood Glucose ; Hypoglycemic Agents ; Plant Extracts ; Streptozocin (5W494URQ81)
Language	English
Publishing date	2019-02-19
Publishing country	France
Document type	Journal Article
ZDB-ID	392415-4
ISSN	1950-6007 ; 0753-3322 ; 0300-0893
ISSN (online)	1950-6007
ISSN	0753-3322 ; 0300-0893
DOI	10.1016/j.biopha.2019.01.059
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Article: Antidiabetic activity of root tubers of Asparagus gonoclados Baker in streptozotocin induced diabetic rats

Rajasekhar, Allagadda / Peddanna, Kotha / Vedasree, Nalluri / Munirajeswari, Pasupuleti / Nagaraju, Nagoji / Badri, Kameswara Rao / Chippada, Appa Rao

Journal of ethnopharmacology. 2019 Oct. 05, v. 242

2019

Abstract: Asparagus gonoclados Baker is a traditional folk remedy used for diabetes, diuretic, galactogogue, gastric ulcer activities etc.The present investigation was intended to evaluate the beneficial effect of the A.gonoclados (Lilliaceae) root tubers against ... ...

Abstract	Asparagus gonoclados Baker is a traditional folk remedy used for diabetes, diuretic, galactogogue, gastric ulcer activities etc.The present investigation was intended to evaluate the beneficial effect of the A.gonoclados (Lilliaceae) root tubers against diabetes mellitus.Different solvent extracts of root tubers of A. gonoclados were used to study the antihyperglycemic activity in streptozotocin (45 mg/kg.wt) induced diabetic rats. Oral glucose tolerance test was performed in diabetic and normal rats treated with A.gonoclados. Total phenolic content (TPC), total flavanoid content (TFC) and total steroidal saponins content (TSSC) were measured in different solvent extracts. Following bioassay guided fractionation method antihyperglycemic active fraction of A. gonoclados (AGAF) was isolated from the ethanol extract (AGEE) by silica gel column chromatography. We further tested relationship between insulin stimulation effect and the influence of active fraction on K+-ATP and Ca2+ channels opening in normal and diabetic rats. The characterization of AGAF was carried out by LC-ESI-MS/MS.Among the different solvent extracts, the ethanol extract (AGEE) at a dose of 500 mg/kg b.wt has produced maximum (67%) reduction in fasting blood glucose levels (FBG) in diabetic treated rats after 6 h of oral administration when compared to the standard drug glibenclamide (40%). AGEE also showed dose-dependent inhibitory effect on the activities of α-glucosidase (74.73%) and α-amylase (76.47%), which is comparable to the activity of standard drug acarbose (88.42%). AGEE was found to have the richest quantity of TPCs (138.4 ± 0.39 μg/mg gallic acid equivalents), TFCs (64.8 ± 0.54 μg/mg quercetin equivalents) and TSSCs (12.9 ± 0.11μg/mg sarasapogenin equivalents). We identified 8 potential antihyperglycemic compounds in AGAF by LC-ESI-MS/MS analysis.From our current study we confirm that A. gonoclados root tubers have potent antihyperglycemic activity and it can be a unique drug/formulation for the management of diabetes mellitus.
Keywords	acarbose ; alpha-amylase ; alpha-glucosidase ; animal disease models ; bioassays ; blood glucose ; calcium channels ; diabetes mellitus ; dose response ; drugs ; electrospray ionization mass spectrometry ; enzyme activity ; ethanol ; fasting ; fractionation ; gallic acid ; gel chromatography ; glibenclamide ; glucose tolerance tests ; glycemic effect ; insulin ; oral administration ; quercetin ; rats ; solvents ; steroid saponins ; stomach ulcers ; streptozotocin ; traditional medicine ; tubers
Language	English
Dates of publication	2019-1005
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2019.112027
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