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  1. Book ; Thesis: Neue und zoonotische Virusinfektionen bei landwirtschaftlichen Nutztieren - Identifikation, Infektionsverlauf und molekulare Mechanismen

    Bächlein, Christine

    2021  

    Author's details vorgelegt von Christine Bächlein, Ph. D
    Language German
    Edition 1. Auflage
    Publisher DVG Service GmbH
    Publishing place Gießen
    Publishing country Germany
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Habilitationsschrift, Stiftung Tierärztliche Hochschule Hannover, 2020
    HBZ-ID HT020884719
    ISBN 978-3-86345-568-2 ; 3-86345-568-1
    Database Catalogue ZB MED Nutrition, Environment, Agriculture

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    In stock of ZB MED Bonn / Germany

    Shelf markLoan statusLocation
    218/110 available for loan (reading room) Freihandmagazin (U1)

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  2. Book ; Online ; Thesis: Hepatitis E virus in German domestic pigs

    Bächlein, Christine

    occurrence and prevalence

    2011  

    Author's details Christine Bächlein
    Language English
    Publisher Bibliothek der Tierärztlichen Hochschule Hannover
    Publishing place Hannover
    Publishing country Germany
    Document type Book ; Online ; Thesis
    Thesis / German Habilitation thesis Hannover, Tierärztliche Hochsch., Diss., 2011
    Note Gedr. Ausg. unter ISBN 978-3-86345-056-4 ersch. ; Open Access
    HBZ-ID HT017172507
    ISBN 978-3-86345-056-4 ; 3-86345-056-6
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  3. Book ; Online ; Thesis: Neue und zoonotische Virusinfektionen bei landwirtschaftlichen Nutztieren – Identifikation, Infektionsverlauf und molekulare Mechanismen

    Bächlein, Christine [Verfasser]

    2021  

    Author's details Christine Bächlein
    Keywords Landwirtschaft, Veterinärmedizin ; Agriculture, Veterinary Science
    Subject code sg630
    Language German
    Publisher Tierärztliche Hochschule Hannover
    Publishing place Hannover
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  4. Article ; Online: Isolation of 15 hepatitis E virus strains lacking ORF1 rearrangements from wild boar and pig organ samples and efficient replication in cell culture.

    Gremmel, Nele / Keuling, Oliver / Becher, Paul / Baechlein, Christine

    Transboundary and emerging diseases

    2022  Volume 69, Issue 5, Page(s) e2617–e2628

    Abstract: As a zoonotic pathogen, the hepatitis E virus (HEV) leads to numerous infections in humans with different clinical manifestations. Especially genotype 3, as causative agent of a foodborne zoonosis, is transmitted to humans by ingestion of undercooked or ... ...

    Abstract As a zoonotic pathogen, the hepatitis E virus (HEV) leads to numerous infections in humans with different clinical manifestations. Especially genotype 3, as causative agent of a foodborne zoonosis, is transmitted to humans by ingestion of undercooked or raw meat containing liver from HEV-infected animals. Although the virus' prevalence and dissemination in hosts like wild boar and pig have been well characterized, HEV is greatly understudied on a molecular level and reliable cell culture models are lacking. For this reason, the present study concentrated on the isolation and subsequent characterization of porcine HEV from tissue samples derived from wild boar and domestic pigs: 222 wild boars hunted in Northern Germany were investigated for the presence of HEV RNA with a detection rate of 5.9%. Three additional HEV-positive wild boar liver samples as well as an HEV-positive spleen and a positive kidney from domestic pigs were included. After inoculation of positive samples onto the human hepatoma cell line PLC/PRF/5, cells were grown for several weeks. Successful isolation was confirmed by RT-qPCR, virus passage, immunofluorescence staining and titration. Overall, 15 strains from a total of 18 RNA-positive organ samples could be obtained and viral loads >10
    MeSH term(s) Animals ; Cell Culture Techniques/veterinary ; Hepatitis E/epidemiology ; Hepatitis E/veterinary ; Hepatitis E virus ; Humans ; Phylogeny ; RNA ; RNA, Viral/genetics ; Sus scrofa ; Swine ; Swine Diseases/epidemiology
    Chemical Substances RNA, Viral ; RNA (63231-63-0)
    Language English
    Publishing date 2022-06-22
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2414822-2
    ISSN 1865-1682 ; 1865-1674
    ISSN (online) 1865-1682
    ISSN 1865-1674
    DOI 10.1111/tbed.14608
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Isolation of 15 hepatitis E virus strains lacking ORF1 rearrangements from wild boar and pig organ samples and efficient replication in cell culture

    Gremmel, Nele / Keuling, Oliver / Becher, Paul / Baechlein, Christine

    Transboundary and emerging diseases. 2022 Sept., v. 69, no. 5

    2022  

    Abstract: As a zoonotic pathogen, the hepatitis E virus (HEV) leads to numerous infections in humans with different clinical manifestations. Especially genotype 3, as causative agent of a foodborne zoonosis, is transmitted to humans by ingestion of undercooked or ... ...

    Abstract As a zoonotic pathogen, the hepatitis E virus (HEV) leads to numerous infections in humans with different clinical manifestations. Especially genotype 3, as causative agent of a foodborne zoonosis, is transmitted to humans by ingestion of undercooked or raw meat containing liver from HEV‐infected animals. Although the virus’ prevalence and dissemination in hosts like wild boar and pig have been well characterized, HEV is greatly understudied on a molecular level and reliable cell culture models are lacking. For this reason, the present study concentrated on the isolation and subsequent characterization of porcine HEV from tissue samples derived from wild boar and domestic pigs: 222 wild boars hunted in Northern Germany were investigated for the presence of HEV RNA with a detection rate of 5.9%. Three additional HEV‐positive wild boar liver samples as well as an HEV‐positive spleen and a positive kidney from domestic pigs were included. After inoculation of positive samples onto the human hepatoma cell line PLC/PRF/5, cells were grown for several weeks. Successful isolation was confirmed by RT‐qPCR, virus passage, immunofluorescence staining and titration. Overall, 15 strains from a total of 18 RNA‐positive organ samples could be obtained and viral loads >10⁹ RNA copies/ml were measured in cell culture supernatants. Accordingly, 83.3% of the HEV RNA‐positive samples contained infectious hepatitis E viral particles and therefore must be considered as a potential source for human infections. Phylogenetic analyses revealed that all isolated strains belong to genotype 3. Further genetic characterization showed a high degree of sequence variability, but no sequence insertions, in the hypervariable region within the open reading frame 1.
    Keywords Orthohepevirus A ; RNA ; cell culture ; cell lines ; fluorescent antibody technique ; genetic variation ; genotype ; hepatitis A ; hepatitis E ; hepatoma ; humans ; ingestion ; kidneys ; liver ; pathogens ; phylogeny ; raw meat ; spleen ; swine ; titration ; viruses ; wild boars ; zoonoses ; Germany
    Language English
    Dates of publication 2022-09
    Size p. e2617-e2628.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 2414822-2
    ISSN 1865-1682 ; 1865-1674
    ISSN (online) 1865-1682
    ISSN 1865-1674
    DOI 10.1111/tbed.14608
    Database NAL-Catalogue (AGRICOLA)

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  6. Book ; Audio / Video ; Online: Neutralizing properties of porcine antibodies against the hepatitis E virus – establishment of a cell culture-based assay

    Gremmel, Nele / Keuling, Oliver / Johne, Reimar / Baechlein, Christine / Becher, Paul

    2023  

    Keywords Text ; ddc:570
    Language English
    Publishing date 2023-03-28/2023-03-31
    Publishing country de
    Document type Book ; Audio / Video ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Book ; Online ; Thesis: Hepatitis E virus in German domestic pigs: occurrence and prevalence

    Bächlein, Christine

    2011  

    Abstract: Hepatitis E-Virus, Hausschweine, Verbreitung, Hepatitis E virus, domestic pigs, prevalence. - Das Hepatitis E-Virus (HEV) ist der Erreger einer akuten, in der Regel selbstlimitierenden Hepatitis. In Entwicklungsländern wird das Virus über kontaminiertes ... ...

    Author's details vorgelegt von Christine Bächlein
    Abstract Hepatitis E-Virus, Hausschweine, Verbreitung, Hepatitis E virus, domestic pigs, prevalence. - Das Hepatitis E-Virus (HEV) ist der Erreger einer akuten, in der Regel selbstlimitierenden Hepatitis. In Entwicklungsländern wird das Virus über kontaminiertes Trinkwasser übertragen. Dabei handelt es sich hauptsächlich um den Genotyp 1 und 2. Auch in Industriestaaten wurden vereinzelt Infektionen mit diesen beiden Genotypen beschrieben, vornehmlich bei Personen, die aus Endemiegebieten zurückgekehrt waren, so dass man hier von einer klassischen reiseassoziierten Erkrankung ausging. Zunehmend traten jedoch in den letzten Jahren auch HEV-Infektionen bei Personen ohne entsprechende Reisehistorie auf, so dass die Epidemiologie dieser Infektionskrankheit in Industriestaaten überdacht werden musste und für HEV ein Erregerreservoir in Tieren diskutiert wurde. Untersuchungen haben gezeigt, dass in Europa vor allem Haus- und Wildschweine als Virusträger fungieren. Die zoonotische Übertragung des Virus wird dabei durch die große Ähnlichkeit humaner und porziner HEV-Genomsequenzen gestützt. Um das Infektionsrisiko für den Menschen abschätzen zu können, sind detaillierte Kenntnisse über die Verbreitung des Virus in der Schweinepopulation in Deutschland unerlässlich. Ziel der vorliegenden PhD-Arbeit war es, das Vorhandensein von HEV in der Hausschweinepopulation in Deutschland und die Auswirkungen der Virusinfektion im natürlichen Wirt zu charakterisieren. Mit Hilfe eines neu entwickelten Antikörper-ELISA wurden Serumproben aus 11 verschiedenen Bundesländern untersucht. Von 1072 untersuchten Seren wiesen 49,8 % HEV-spezifisches Immunglobulin G auf. Auf Bestandsebene hatten gut dreiviertel der untersuchten Bestände (78,1 %) bereits Kontakt zum Virus gehabt. Ein Vergleich des ELISA mit zwei kommerziellen ....
    Language English
    Size Online-Ressource (VIII, 94 S., 1.619 kb, text), Ill., graph. Darst.
    Edition 1. Aufl.
    Publisher Tierärztl. Hochsch ; DVG
    Publishing place Hannover ; Gießen
    Document type Book ; Online ; Thesis
    Thesis / German Habilitation thesis Zugl.: Hannover, Tierärztl. Hochsch., Inst. of Virology, Department of Infectious Diseases, Diss., 2011
    Note Sprache der Zusammenfassung: Deutsch ; Sprache der Zusammenfassung: Englisch
    ISBN 9783863450564 ; 3863450566
    Database Special collection on veterinary medicine and general parasitology

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  8. Book ; Online ; Thesis: Hepatitis E virus in German domestic pigs: occurrence and prevalence

    Bächlein, Christine

    2011  

    Abstract: Hepatitis E-Virus, Hausschweine, Verbreitung, Hepatitis E virus, domestic pigs, prevalence. - Das Hepatitis E-Virus (HEV) ist der Erreger einer akuten, in der Regel selbstlimitierenden Hepatitis. In Entwicklungsländern wird das Virus über kontaminiertes ... ...

    Author's details vorgelegt von Christine Bächlein
    Abstract Hepatitis E-Virus, Hausschweine, Verbreitung, Hepatitis E virus, domestic pigs, prevalence. - Das Hepatitis E-Virus (HEV) ist der Erreger einer akuten, in der Regel selbstlimitierenden Hepatitis. In Entwicklungsländern wird das Virus über kontaminiertes Trinkwasser übertragen. Dabei handelt es sich hauptsächlich um den Genotyp 1 und 2. Auch in Industriestaaten wurden vereinzelt Infektionen mit diesen beiden Genotypen beschrieben, vornehmlich bei Personen, die aus Endemiegebieten zurückgekehrt waren, so dass man hier von einer klassischen reiseassoziierten Erkrankung ausging. Zunehmend traten jedoch in den letzten Jahren auch HEV-Infektionen bei Personen ohne entsprechende Reisehistorie auf, so dass die Epidemiologie dieser Infektionskrankheit in Industriestaaten überdacht werden musste und für HEV ein Erregerreservoir in Tieren diskutiert wurde. Untersuchungen haben gezeigt, dass in Europa vor allem Haus- und Wildschweine als Virusträger fungieren. Die zoonotische Übertragung des Virus wird dabei durch die große Ähnlichkeit humaner und porziner HEV-Genomsequenzen gestützt. Um das Infektionsrisiko für den Menschen abschätzen zu können, sind detaillierte Kenntnisse über die Verbreitung des Virus in der Schweinepopulation in Deutschland unerlässlich. Ziel der vorliegenden PhD-Arbeit war es, das Vorhandensein von HEV in der Hausschweinepopulation in Deutschland und die Auswirkungen der Virusinfektion im natürlichen Wirt zu charakterisieren. Mit Hilfe eines neu entwickelten Antikörper-ELISA wurden Serumproben aus 11 verschiedenen Bundesländern untersucht. Von 1072 untersuchten Seren wiesen 49,8 % HEV-spezifisches Immunglobulin G auf. Auf Bestandsebene hatten gut dreiviertel der untersuchten Bestände (78,1 %) bereits Kontakt zum Virus gehabt. Ein Vergleich des ELISA mit zwei kommerziellen ....
    Language English
    Size Online-Ressource (VIII, 94 S., 1.619 kb, text), Ill., graph. Darst.
    Edition 1. Aufl.
    Publisher Tierärztl. Hochsch ; DVG
    Publishing place Hannover ; Gießen
    Document type Book ; Online ; Thesis
    Thesis / German Habilitation thesis Tierärztl. Hochsch., Inst. of Virology, Department of Infectious Diseases, Diss.--Hannover, 2011
    ISBN 9783863450564 ; 3863450566
    Database Former special subject collection: coastal and deep sea fishing

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  9. Article ; Online: Hepatitis E virus neutralization by porcine serum antibodies.

    Gremmel, Nele / Keuling, Oliver / Eiden, Martin / Groschup, Martin H / Johne, Reimar / Becher, Paul / Baechlein, Christine

    Journal of clinical microbiology

    2023  Volume 61, Issue 11, Page(s) e0037323

    Abstract: The consumption of raw or undercooked meat products poses a serious risk for human hepatitis E virus (HEV) infections. In many high-income countries, domestic pigs and wild boars represent the main animal reservoirs for HEV and are usually identified by ... ...

    Abstract The consumption of raw or undercooked meat products poses a serious risk for human hepatitis E virus (HEV) infections. In many high-income countries, domestic pigs and wild boars represent the main animal reservoirs for HEV and are usually identified by reverse transcription-PCR and antibody enzyme-linked immunosorbent assay (ELISA). In order to characterize the humoral immune response in more detail, a cell culture-based serum neutralization assay using a culture-adapted HEV strain was established here. Measurement of neutralizing antibodies was only possible after removing the viral quasi-envelope by detergent treatment. Serum samples of 343 wild boars from Northern Germany were first analyzed for anti-HEV IgG using an in-house ELISA, resulting in 19% positive samples. Subsequently, a subset of 41 representative samples was tested with the neutralization assay, and the results correlated well with those obtained by ELISA. Not only the human HEV strain 47832c but also two porcine HEV strains were shown to be neutralized by porcine serum antibodies. Neutralizing activity was also found in samples containing both HEV-specific antibodies and HEV RNA. Testing of serum samples derived from two experimentally infected domestic pigs showed a steep increase in neutralizing activity at 24 or 51 days post infection, dependent on the used infectious dose. The developed assay can be useful for characterization of the humoral immune response after HEV infection and for assessing the efficiency of HEV vaccine candidates.
    MeSH term(s) Swine ; Animals ; Humans ; Hepatitis E virus/genetics ; Hepatitis E ; Sus scrofa/genetics ; Hepatitis Antibodies ; Enzyme-Linked Immunosorbent Assay ; Swine Diseases ; RNA, Viral
    Chemical Substances Hepatitis Antibodies ; RNA, Viral
    Language English
    Publishing date 2023-10-12
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 390499-4
    ISSN 1098-660X ; 0095-1137
    ISSN (online) 1098-660X
    ISSN 0095-1137
    DOI 10.1128/jcm.00373-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1188: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  10. Article ; Online: No evidence for zoonotic hepatitis E virus infection through dairy milk in Germany.

    Baechlein, Christine / Becher, Paul

    Hepatology (Baltimore, Md.)

    2017  Volume 65, Issue 1, Page(s) 394–395

    Language English
    Publishing date 2017-01
    Publishing country United States
    Document type Letter
    ZDB-ID 604603-4
    ISSN 1527-3350 ; 0270-9139
    ISSN (online) 1527-3350
    ISSN 0270-9139
    DOI 10.1002/hep.28863
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1660: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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