LIVIVO - Search results -

Search results

Result 1 - 10 of total 143

Search options

Article ; Online: High-Dimensional Modeling for Cytometry: Building Rock Solid Models Using GemStone™ and Verity Cen-se'™ High-Definition t-SNE Mapping.

Bruce Bagwell, C

Methods in molecular biology (Clifton, N.J.)

2017 Volume 1678, Page(s) 11–36

Abstract: This chapter outlines how to approach the complex tasks associated with designing models for high-dimensional cytometry data. Unlike gating approaches, modeling lends itself to automation and accounts for measurement overlap among cellular populations. ... ...

Abstract	This chapter outlines how to approach the complex tasks associated with designing models for high-dimensional cytometry data. Unlike gating approaches, modeling lends itself to automation and accounts for measurement overlap among cellular populations. Designing these models is now easier because of a new technique called high-definition t-SNE mapping. Nontrivial examples are provided that serve as a guide to create models that are consistent with data.
MeSH term(s)	Algorithms ; Animals ; Computational Biology/methods ; Data Interpretation, Statistical ; Flow Cytometry/methods ; Flow Cytometry/standards ; Gene Expression Profiling/methods ; Humans ; Immunophenotyping/methods ; Models, Statistical ; Organ Specificity ; Reproducibility of Results ; Workflow
Language	English
Publishing date	2017-03-23
Publishing country	United States
Document type	Journal Article ; Review
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-4939-7346-0_2
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Analysis of the multiparametric cell cycle data.

Jacobberger, James W / Sramkoski, R Michael / Stefan, Tammy / Bray, Chris / Bagwell, C Bruce

Methods in cell biology

2024 Volume 186, Page(s) 271–309

Abstract: This chapter was originally written in 2011. The idea was to give some history of cell cycle analysis before and after flow cytometry became widely accessible; provide references to educational material for single parameter DNA content analysis, ... ...

Abstract	This chapter was originally written in 2011. The idea was to give some history of cell cycle analysis before and after flow cytometry became widely accessible; provide references to educational material for single parameter DNA content analysis, introduce and discuss multiparameter cell cycle analysis in a methodological style, and in a casual style, discuss aspects of the work over the last 40years that we have given thought, performing some experiments, but didn't publish. It feels like there is a linear progression that moves from counting cells for growth curves, to counting labeled mitotic cells by autoradiography, to DNA content analysis, to cell cycle states defined by immunofluorescence plus DNA content analysis, to extraction of cell cycle expression profiles, and finally to probability state modeling, which should be the "right" way to analyze cytometric cell cycle data. This is the sense of this chapter. In 2023, we have updated it, but the exciting, expansive aspects brought about by spectral and mass cytometry are still young and developing, and thus have not been vetted, reviewed, and presented in mature form.
MeSH term(s)	Cell Cycle ; Humans ; Flow Cytometry/methods ; Animals ; DNA
Chemical Substances	DNA (9007-49-2)
Language	English
Publishing date	2024-03-26
Publishing country	United States
Document type	Journal Article ; Review
ISSN	0091-679X
ISSN	0091-679X
DOI	10.1016/bs.mcb.2024.02.021
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Breaking the dimensionality barrier.

Bagwell, C Bruce

Methods in molecular biology (Clifton, N.J.)

2011 Volume 699, Page(s) 31–51

Abstract: Recent advances in biotechnology have resulted in cytometers capable of performing numerous correlated measurements of cells, often exceeding ten. In the near future, it is likely that this number will increase by fivefold and perhaps even higher. ... ...

Abstract	Recent advances in biotechnology have resulted in cytometers capable of performing numerous correlated measurements of cells, often exceeding ten. In the near future, it is likely that this number will increase by fivefold and perhaps even higher. Traditional analysis strategies based on examining one measurement versus another are not suitable for high-dimensional data analysis because the number of measurement combinations expands geometrically with dimension, forming a kind of complexity barrier. This dimensionality barrier limits cytometry and other technologies from reaching their maximum potential in visualizing and analyzing important information embedded in high-dimensional data.This chapter describes efforts to break through this barrier and allow the visualization and analysis of any number of measurements with a new paradigm called Probability State Modeling (PSM). This new system creates a virtual progression variable based on probability that relates all measurements. PSM can produce a single graph that conveys more information about a sample than hundreds of traditional histograms. These PSM overlays reveal the rich interplay of phenotypic changes in cells as they differentiate. The end result is a deeper appreciation of the molecular genetic underpinnings of ontological processes in complex populations such as found in bone marrow and peripheral blood.Eventually these models will help investigators better understand normal and abnormal cellular progressions and will be a valuable general tool for the analysis and visualization of high-dimensional data.
MeSH term(s)	Animals ; Bone Marrow Cells/cytology ; Bone Marrow Cells/immunology ; Cell Lineage/physiology ; Flow Cytometry/instrumentation ; Flow Cytometry/methods ; Humans ; Models, Theoretical ; Statistics as Topic
Language	English
Publishing date	2011
Publishing country	United States
Document type	Journal Article ; Review
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-61737-950-5_2
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Resident training.

Bagwell, Charles

Bulletin of the American College of Surgeons

2006 Volume 91, Issue 4, Page(s) 66

MeSH term(s)	General Surgery/education ; Government Regulation ; Internship and Residency ; United States ; Workload/legislation & jurisprudence
Language	English
Publishing date	2006-04
Publishing country	United States
Document type	Letter
ZDB-ID	390409-x
ISSN	0002-8045
ISSN	0002-8045
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 513: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Analytical procedures

Bagwell, Chris / Quick, Linda A / Vandervelde, Scott D

Advances in accounting education : teaching and curriculum innovations Vol. 20 , p. 51-78

an in-class exercise

2017 Volume 20, Page(s) 51–78

Author's details	Chris Bagwell, Linda A. Quick and Scott D. Vandervelde
Language	English
Publisher	Emerald
Publishing place	Bingley
Document type	Article
ZDB-ID	2147206-3
ISSN	1085-4622
Database	ECONomics Information System

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Automated analysis of flow cytometric data for CD34+ stem cell enumeration using a probability state model.

Herbert, Donald J / Miller, David T / Bruce Bagwell, C

Cytometry. Part B, Clinical cytometry

2012 Volume 82, Issue 5, Page(s) 313–318

Abstract: Background: Flow Cytometry is widely used for enumeration of hematopoietic stem cell (SC) levels in bone marrow, cord blood, peripheral blood, and apheresis products. The ISHAGE single-platform gating method is considered by many to be the standard for ... ...

Abstract	Background: Flow Cytometry is widely used for enumeration of hematopoietic stem cell (SC) levels in bone marrow, cord blood, peripheral blood, and apheresis products. The ISHAGE single-platform gating method is considered by many to be the standard for CD34+ SC enumeration. However, attempts at uniform application of this ISHAGE method have met with only partial success. We propose an automated, multivariate classification approach for SC analysis based on Probability State Modeling™ (PSM). In this study, we compare the results from automated PSM analysis with manual ISHAGE gating analysis as performed by a trained analyst. Methods: A total of 258 samples were assayed on BD FACSCanto II flow cytometers using a stain-lyse-no-wash technique. Populations were defined using CD34, CD45, 7-AAD, and light scatter. BD TruCount™ bead tubes were used for absolute SC concentrations. A PSM was designed to classify events into beads, debris, intact-dead cells, and intact-live SC; run unattended and record results. Results: The ISHAGE and PSM methods show excellent agreement in estimating the concentration of #SC/μL: slope = 1.009, r² = 0.999. Bland-Altman Analysis for the SC concentration has an average difference (bias) of 2.018 SC/μL. The 95% confidence interval is from -59.350 to 63.380 SC/μL. The operator-to-operator agreement using PSM is perfect: r² = 1.000. Conclusions: Automated PSM analysis of SC listmode data produces results that agree strongly with ISHAGE gate-based results. The PSM approach provides higher reproducibility, objectivity, and speed with accuracy at least equivalent to the ISHAGE method.
MeSH term(s)	Antigens, CD34/metabolism ; Automation, Laboratory ; Cell Count ; Data Interpretation, Statistical ; Flow Cytometry/methods ; Humans ; Linear Models ; Probability ; Stem Cells/cytology ; Stem Cells/metabolism
Chemical Substances	Antigens, CD34
Language	English
Publishing date	2012-09
Publishing country	United States
Document type	Journal Article
ZDB-ID	2099657-3
ISSN	1552-4957 ; 1552-4949 ; 0196-4763
ISSN (online)	1552-4957
ISSN	1552-4949 ; 0196-4763
DOI	10.1002/cyto.b.21032
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1688: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Automated quantitation of fetomaternal hemorrhage by flow cytometry for HbF-containing fetal red blood cells using probability state modeling.

Wong, L / Hunsberger, B C / Bruce Bagwell, C / Davis, B H

International journal of laboratory hematology

2013 Volume 35, Issue 5, Page(s) 548–554

Abstract: Background: Flow cytometric methods (FCMs) are the contemporary standard for fetal red blood cell (RBC) quantitation and fetomaternal hemorrhage (FMH) detection. FCM provides greater sensitivity and repeatability relative to manual microscopic Kleihauer- ...

Abstract	Background: Flow cytometric methods (FCMs) are the contemporary standard for fetal red blood cell (RBC) quantitation and fetomaternal hemorrhage (FMH) detection. FCM provides greater sensitivity and repeatability relative to manual microscopic Kleihauer-Betke methods. FCM assays are not totally objective, employing subjective manual gating of fetal RBCs with measureable interobserver imprecision. We investigated Probability State Modeling to automate analysis of fetal RBCs using an assay for hemoglobin F (HbF)-containing RBCs. Methods: Two hundred human bloods were processed using the FMH QuikQuant™ assay (Trillium Diagnostics, Brewer, ME, USA). A Probability State Model (PSM) was designed to enumerate fetal RBCs by selecting the three RBCs subpopulation based on differences in intensity levels of several parameters. The GemStone™ program uses a PSM that requires no operator intervention. Routine manual analysis by experienced users was performed, along with replicate analyses for both methods. Results: The PSM by GemStone™ correlates strongly with the expert manual analysis, r(2) = 0.9986. The mean absolute difference of the FMH results between GemStone™ and manual 'expert' analysis was 0.04% with no intermethod bias detected. Manual gating demonstrated coefficient of variations (CVs) of 10.6% for intra-analyst replicates and 22.6% for interanalyst imprecision. The interanalyst agreement in GemStone™ is a perfect correlation, r(2) = 1.00, and no imprecision with a 0.00% CV. Conclusion: Automated PSM analysis of fetal RBCs strongly correlates with expert traditional manual analysis. PSM enumerates fetal RBCs accurately with significantly greater objectivity and lower imprecision than the traditional manual gating method. Thus, PSM provides a means to markedly improve interlaboratory variance with FMH assays based upon subjective gating strategies.
MeSH term(s)	Erythrocytes/metabolism ; Female ; Fetal Blood/cytology ; Fetal Hemoglobin/metabolism ; Fetomaternal Transfusion/diagnosis ; Flow Cytometry/methods ; Humans ; Models, Statistical ; Pregnancy ; Probability ; Reproducibility of Results
Chemical Substances	Fetal Hemoglobin (9034-63-3)
Language	English
Publishing date	2013-10
Publishing country	England
Document type	Journal Article
ZDB-ID	2268590-X
ISSN	1751-553X ; 1751-5521 ; 0141-9854
ISSN (online)	1751-553X
ISSN	1751-5521 ; 0141-9854
DOI	10.1111/ijlh.12060
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1499: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article: Hyperlog-a flexible log-like transform for negative, zero, and positive valued data.

Bagwell, C Bruce

Cytometry. Part A : the journal of the International Society for Analytical Cytology

2005 Volume 64, Issue 1, Page(s) 34–42

Abstract: Background: The remarkable success of cytometry over the past 30 years is largely due to its uncanny ability to display populations that vastly differ in numbers and fluorescence intensities on one scale. The log transform implemented in hardware as a ... ...

Abstract	Background: The remarkable success of cytometry over the past 30 years is largely due to its uncanny ability to display populations that vastly differ in numbers and fluorescence intensities on one scale. The log transform implemented in hardware as a log amplifier or in software normalizes signals or channels so that these populations appear as clearly discernible peaks. With the advent of multiple fluorescence cytometry, spectral crossover compensation of these signals has been necessary to properly interpret the data. Unfortunately, because compensation is a subtractive process, it can produce negative and zero valued data. The log transform is undefined for these values and, as a result, forces computer algorithms to truncate these values, creating a few problems for cytometrists. Data truncation biases displays making properly compensated data appear undercompensated; thus, enticing many operators to overcompensate their data. Also, events truncated into the first histogram channel are not normally visible with typical two-dimensional graphic displays, thus hiding a large number of events and obscuring the true proportionality of negative distributions. In addition, the log transform creates unequal binning that can dramatically distort negative population distributions. Methods and results: The HyperLog transform is a log-like transform that admits negative, zero, and positive values. The transform is a hybrid type of transform specifically designed for compensated data. One of its parameters allows it to smoothly transition from a logarithmic to linear type of transform that is ideal for compensated data. Conclusions: The HyperLog transform is easily implemented in computer systems and results in display systems that present compensated data in an unbiased manner.
MeSH term(s)	Algorithms ; Computer Simulation ; Flow Cytometry/methods ; Mathematics ; Signal Processing, Computer-Assisted ; Software
Language	English
Publishing date	2005-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	2099868-5
ISSN	1552-4930 ; 1552-4922 ; 0196-4763
ISSN (online)	1552-4930
ISSN	1552-4922 ; 0196-4763
DOI	10.1002/cyto.a.20114
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1688: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 68: Show issues

Order via subito

Details ▾

Article: DNA histogram analysis for node-negative breast cancer.

Bagwell, C Bruce

Cytometry. Part A : the journal of the International Society for Analytical Cytology

2004 Volume 58, Issue 1, Page(s) 76–78

MeSH term(s)	Breast Neoplasms/classification ; Breast Neoplasms/diagnosis ; Breast Neoplasms/genetics ; DNA, Neoplasm/analysis ; Flow Cytometry/instrumentation ; Flow Cytometry/methods ; Flow Cytometry/standards ; Humans ; Lymph Nodes/pathology ; Lymphatic Metastasis/pathology ; Ploidies
Chemical Substances	DNA, Neoplasm
Language	English
Publishing date	2004-03
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2099868-5
ISSN	1552-4930 ; 1552-4922 ; 0196-4763
ISSN (online)	1552-4930
ISSN	1552-4922 ; 0196-4763
DOI	10.1002/cyto.a.90004
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1688: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 68: Show issues

Order via subito

Details ▾

Book ; Online: Friendships in childhood & adolescence

Bagwell, Catherine / Schmidt, Michelle E

(The Guilford series on social and emotional development)

2011

Abstract: Highly readable and comprehensive, this volume explores the significance of friendship for social, emotional, and cognitive development from early childhood through adolescence. The authors trace how friendships change as children age and what specific ... ...

Title variant	Friendships in childhood and adolescence
Institution	ebrary, Inc
Author's details	Catherine L. Bagwell, Michelle E. Schmidt
Series title	The Guilford series on social and emotional development
Abstract	Highly readable and comprehensive, this volume explores the significance of friendship for social, emotional, and cognitive development from early childhood through adolescence. The authors trace how friendships change as children age and what specific functions these relationships play in promoting adjustment and well-being. Compelling topics include the effects of individual differences on friendship quality, how friendship quality can be assessed, and ways in which certain friendships may promote negative outcomes. Examining what clinicians, educators, and parents can do to help children wh
MeSH term(s)	Adolescent ; Child ; Friends ; Interpersonal Relations/Child
Keywords	Friendship in adolescence ; Friendship in children
Language	English
Size	Online-Ressource (xvii, 389 p), ill
Publisher	Guilford Press
Publishing place	New York
Document type	Book ; Online
Note	Includes bibliographical references and index
ISBN	1283238527 ; 128323923X ; 9781283239233 ; 9781609186463 ; 9781283238526 ; 160918646X
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED