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  1. Article ; Online: RCOR1 is targeted by miR-23b-3p to modulate growth, colony formation, migration, and invasion of prostate cancer cells.

    Liu, Chenli / Dong, Zhong / Li, Maozhang / Bai, Guangwei / Zhao, Zhixiang

    International journal of clinical and experimental pathology

    2024  Volume 17, Issue 2, Page(s) 29–38

    Abstract: Objectives: Prostate cancer holds the second-highest incidence rate among all male malignancies, with a noticeable scarcity of effective treatment approaches. The REST Corepressor 1 (RCOR1) protein exhibits elevated expression across various tumors, ... ...

    Abstract Objectives: Prostate cancer holds the second-highest incidence rate among all male malignancies, with a noticeable scarcity of effective treatment approaches. The REST Corepressor 1 (RCOR1) protein exhibits elevated expression across various tumors, acting as an oncogene. Nevertheless, its functions and mechanisms in prostate cancer have yet to be documented. While miR-23 demonstrates reduced expression in prostate cancer, the downstream genes it regulates remain unclear.
    Methods: RT-qPCR and Western blotting assays were utilized to elucidate the mRNA and protein levels of miR-23b-3p and RCOR1. The luciferase reporter assay was employed to unveil the targeting relationship between miR-23b-3p and RCOR1. Additionally, a CCK-8 assay demonstrated cell growth, while colony formation and Transwell assays were performed to observe clone formation, cell migration, and invasion.
    Results: In this study, we observed substantial mRNA and protein levels of RCOR1 in prostate cancer cells such as DU145, PC3, and LNCap. RCOR1 overexpression enhanced the growth, colony formation, migration, and invasion of prostate cancer cells, whereas genetic silencing of RCOR1 suppressed these processes. Bioinformatics analysis identified miR-23b-3p as a potential regulator of RCOR1, and luciferase assays validated RCOR1 as a downstream target of miR-23b-3p. Increasing miR-23b-3p mimics diminished RCOR1's mRNA and protein levels, while raising miR-23b-3p levels boosted RCOR1's expression. Moreover, the stimulatory impact of RCOR1 on prostate cancer cell development could be countered by elevating miR-23b-3p mimics.
    Conclusion: In summary, our findings confirm that RCOR1 is indeed under the influence of miR-23, shedding light on the miR-23/RCOR1 pathway's role in prostate cancer development. This offers novel theoretical and experimental support for comprehending the underlying mechanisms of prostate cancer and for targeted therapeutic avenues.
    Language English
    Publishing date 2024-02-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2418306-4
    ISSN 1936-2625 ; 1936-2625
    ISSN (online) 1936-2625
    ISSN 1936-2625
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Silencing HOXC13 exerts anti-prostate cancer effects by inducing DNA damage and activating cGAS/STING/IRF3 pathway.

    Li, Maozhang / Bai, Guangwei / Cen, Yi / Xie, Qitong / Chen, Jiahong / Chen, Jia / Chen, Qingbiao / Zhong, Weide / Zhou, Xiaobo

    Journal of translational medicine

    2023  Volume 21, Issue 1, Page(s) 884

    Abstract: Background: Advanced prostate cancer (PCa) will develop into castration-resistant prostate cancer (CRPC) and lead to poor prognosis. As the primary subtype of CRPC, CRPC-AR accounts for the major induction of PCa heterogeneity. CRPC-AR is mainly driven ... ...

    Abstract Background: Advanced prostate cancer (PCa) will develop into castration-resistant prostate cancer (CRPC) and lead to poor prognosis. As the primary subtype of CRPC, CRPC-AR accounts for the major induction of PCa heterogeneity. CRPC-AR is mainly driven by 25 transcription factors (TFs), which we speculate may be the key factors driving PCa toward CRPC. Therefore, it is necessary to clarify the key regulator and its molecular mechanism mediating PCa progression.
    Methods: Firstly, we downloaded transcriptomic data and clinical information from TCGA-PRAD. The characteristic gene cluster was identified by PPI clustering, GO enrichment, co-expression correlation and clinical feature analyses for 25 TFs. Then, the effects of 25 TFs expression on prognosis of PCa patients was analyzed using univariate Cox regression, and the target gene was identified. The expression properties of the target gene in PCa tissues were verified using tissue microarray. Meanwhile, the related mechanistic pathway of the target gene was mined based on its function. Next, the target gene was silenced by small interfering RNAs (siRNAs) for cellular function and mechanistic pathway validation. Finally, CIBERSORT algorithm was used to analyze the infiltration levels of 22 immune cells in PCa patients with low and high expression of target gene, and validated by assaying the expression of related immunomodulatory factor.
    Results: We found that HOX family existed independently in 25 TFs, among which HOXC10, HOXC12 and HOXC13 had unique clinical features and the PCa patients with high HOXC13 expression had the worst prognosis. In addition, HOXC13 was highly expressed in tumor tissues and correlated with Gleason score and pathological grade. In vitro experiments demonstrated that silencing HOXC13 inhibited 22RV1 and DU145 cell function by inducing cellular DNA damage and activating cGAS/STING/IRF3 pathway. Immune infiltration analysis revealed that high HOXC13 expression suppressed infiltration of γδ T cells and plasma cells and recruited M2 macrophages. Consistent with these results, silencing HOXC13 up-regulated the transcriptional expression of IFN-β, CCL2, CCL5 and CXCL10.
    Conclusion: HOXC13 regulates PCa progression by mediating the DNA damage-induced cGAS/STING/IRF3 pathway and remodels TIME through regulation of the transcription of the immune factors IFN-β, CCL2, CCL5 and CXCL10.
    MeSH term(s) Male ; Humans ; Prostatic Neoplasms, Castration-Resistant/genetics ; Prostatic Neoplasms, Castration-Resistant/metabolism ; Prostatic Neoplasms, Castration-Resistant/pathology ; Gene Expression Regulation, Neoplastic ; Transcription Factors/metabolism ; Nucleotidyltransferases/genetics ; Nucleotidyltransferases/metabolism ; DNA Damage ; Interferon Regulatory Factor-3/genetics ; Interferon Regulatory Factor-3/metabolism ; Homeodomain Proteins/genetics ; Homeodomain Proteins/metabolism
    Chemical Substances Transcription Factors ; Nucleotidyltransferases (EC 2.7.7.-) ; IRF3 protein, human ; Interferon Regulatory Factor-3 ; HOXC10 protein, human ; Homeodomain Proteins ; HOXC13 protein, human
    Language English
    Publishing date 2023-12-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 2118570-0
    ISSN 1479-5876 ; 1479-5876
    ISSN (online) 1479-5876
    ISSN 1479-5876
    DOI 10.1186/s12967-023-04743-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: CDCS: Cluster-Based Distributed Compressed Sensing to Facilitate QoS Routing in Cognitive Video Sensor Networks.

    Shen, Hang / Li, Lingli / Wang, Tianjing / Bai, Guangwei

    Entropy (Basel, Switzerland)

    2019  Volume 21, Issue 4

    Abstract: Compressed sensing based in-network compression methods which minimize data redundancy are critical to cognitive video sensor networks. However, most existing methods require a large number of sensors for each measurement, resulting in significant ... ...

    Abstract Compressed sensing based in-network compression methods which minimize data redundancy are critical to cognitive video sensor networks. However, most existing methods require a large number of sensors for each measurement, resulting in significant performance degradation in energy efficiency and quality-of-service satisfaction. In this paper, a cluster-based distributed compressed sensing scheme working together with a quality-of-service aware routing framework is proposed to deliver visual information in cognitive video sensor networks efficiently. First, the correlation among adjacent video sensors determines the member nodes that participate in a cluster. On this basis, a sequential compressed sensing approach is applied to determine whether enough measurements are obtained to limit the reconstruction error between decoded signals and original signals under a specified reconstruction threshold. The goal is to maximize the removal of unnecessary traffic without sacrificing video quality. Lastly, the compressed data is transmitted via a distributed spectrum-aware quality-of-service routing scheme, with an objective of minimizing energy consumption subject to delay and reliability constraints. Simulation results demonstrate that the proposed approach can achieve energy-efficient data delivery and reconstruction accuracy of visual information compared with existing quality-of-service routing schemes.
    Language English
    Publishing date 2019-03-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2014734-X
    ISSN 1099-4300 ; 1099-4300
    ISSN (online) 1099-4300
    ISSN 1099-4300
    DOI 10.3390/e21040345
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: [Oxidative stress induces damage to epididymal epithelial tight junction protein ZO-1 and impairs epididymal function in varicocele rats].

    Bai, Guang-Wei / Han, Da-Yu / Yang, Qi-Yun / Xie, Yun / Guo, Ze-Xin / Zhou, Wen-Liang / Deng, Chun-Hua / Sun, Xiang-Zhou

    Zhonghua nan ke xue = National journal of andrology

    2020  Volume 25, Issue 5, Page(s) 302–308

    Abstract: Objective: To investigate oxidative stress-mediated damage to the epididymal epithelial tight junction protein ZO-1 and its impact on epididymal function in varicocele rats.: Methods: We randomly divided 45 male adolescent SD rats into three groups ... ...

    Abstract Objective: To investigate oxidative stress-mediated damage to the epididymal epithelial tight junction protein ZO-1 and its impact on epididymal function in varicocele rats.
    Methods: We randomly divided 45 male adolescent SD rats into three groups of equal number: sham operation (left renal vein exposed and isolated), experimental (left renal vein constricted and collaterals of the left spermatic vein fully ligated), and treatment (60-day intragastric administration of vitamin E at 150 mg/kg/d after modeling). At 60 days after modeling, we observed the histological changes in the left epididymis, detected the expressions of ZO-1 and other tight junction-related proteins by real-time quantitative PCR, immunohistochemistry, immunofluorescence staining and Western blotting, determined sperm motility, and measured the levels of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), methylene dioxyamphetamine (MDA) and α-glucosidase (α-Glu) in the epididymal tissue of the rats.
    Results: Compared with the rats of the sham operation group, those of the experimental group showed disorganized epithelial structure and decreased number of epithelial cells in the left epididymis, with some epithelial cells desquamated into the lumen. The expression of ZO-1 was significantly lower in the experimental than in the sham operation group (P < 0.05) but markedly upregulated after VE treatment (P < 0.05). In comparison with the sham operation group, the animals in the experimental group exhibited remarkably increased content of MDA in the epididymal tissue (［0.41 ± 0.05］ vs ［1.21 ± 0.18］ nmol/mg prot, P < 0.05) but decreased levels of SOD (［814.65 ± 73.64］ vs ［298.62 ± 67.84］ U/mg prot, P < 0.05), T-AOC (［0.84 ± 0.07］ vs ［0.24 ± 0.04］ nmol/mg prot, P < 0.05) and α-Glu (［11.72 ± 2.72］ vs ［5.82 ± 1.24］ U/mg prot, P < 0.05). VE treatment, however, remarkably reduced the content of MDA (［0.69 ± 0.12］ nmol/mg prot) and elevated the levels of SOD (［497.73 ± 48.03］ U/mg prot), T-AOC (［0.42 ± 0.06］ nmol/mg prot) and α-Glu (［9.11 ± 1.91］ U/mg prot) as compared with those in the experimental group (all P < 0.05). The percentage of progressively motile sperm was significantly lower in the experimental than in the sham operation group (［31.33 ± 6.32］% vs ［71.21 ± 5.21］%, P < 0.05), but markedly increased after VE treatment (［60.68 ± 5.31］%, P < 0.05).
    Conclusions: Varicocele reduces the expression of the EETJ protein ZO-1 and impairs epididymal function via oxidative stress, while vitamin E can effectively upregulate the ZO-1 expression and improve epididymal function by decreasing oxidative stress in the epididymis of varicocele rats.
    MeSH term(s) Animals ; Epididymis/physiopathology ; Male ; Oxidative Stress ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Sperm Motility ; Varicocele/physiopathology ; Vitamin E/therapeutic use ; Zonula Occludens-1 Protein/metabolism
    Chemical Substances Tjp1 protein, rat ; Zonula Occludens-1 Protein ; Vitamin E (1406-18-4)
    Language Chinese
    Publishing date 2020-03-25
    Publishing country China
    Document type Journal Article
    ZDB-ID 2267480-9
    ISSN 1009-3591
    ISSN 1009-3591
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6577: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Bandwidth Cost Minimization via User Association for Enterprise WLANs.

    Wan, Xili / Guan, Xinjie / Zhao, Wentian / Bai, Guangwei / Choi, Baek-Young

    Sensors (Basel, Switzerland)

    2018  Volume 18, Issue 12

    Abstract: Enterprise Wireless LANs (E-WLANs) such as airport WiFi, have become a convenient way for Internet access for mobile users. In an E-WLAN, access points (APs) are usually deployed with high-density around the infrastructure to provide sufficient coverage ... ...

    Abstract Enterprise Wireless LANs (E-WLANs) such as airport WiFi, have become a convenient way for Internet access for mobile users. In an E-WLAN, access points (APs) are usually deployed with high-density around the infrastructure to provide sufficient coverage and for a better service, where a mobile user chooses one AP to associate with among multiple available APs in the vicinity. Many studies have been done on developing user association techniques to increase system performance, with various objectives including network throughput maximization, load balancing etc. Our work is unique in that we focused on bandwidth cost minimization via user association from the perspective of the E-WLAN operators. Specifically, by considering the bandwidth demands from mobile users, we modeled the joint user association and cost minimization problem in the heterogeneous E-WLAN with additional constraints from individual bandwidth demands as an optimization problem. To solve the optimization problem efficiently, we propose an approximation algorithm using relaxation and rounding techniques. We prove that the proposed algorithm has performance bound with a constant ratio to the optimization problem. Furthermore, our simulation results exhibit the superiority of our proposed algorithm over prior schemes.
    Language English
    Publishing date 2018-11-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2052857-7
    ISSN 1424-8220 ; 1424-8220
    ISSN (online) 1424-8220
    ISSN 1424-8220
    DOI 10.3390/s18124104
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Book ; Thesis: Load measurements and modeling for distributed multimedia applications in high-speed networks

    Bai, Guangwei

    (Uni Press Hochschulschriften ; 107)

    1999  

    Author's details Guangwei Bai
    Series title Uni Press Hochschulschriften ; 107
    Keywords Leistungsbewertung ; Multimedia ; Verteiltes System ; Hochgeschwindigkeitsnetz ; Lastteilung ; Datenstrom
    Language English
    Size XII, 176 S, 21 cm
    Publisher Lit Verl
    Publishing place Hamburg
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Univ., FB Informatik, Diss.--Hamburg, 1999
    ISBN 3825842797 ; 9783825842796
    Database Former special subject collection: coastal and deep sea fishing

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  7. Book ; Thesis: Load measurements and modeling for distributed multimedia applications in high-speed networks

    Bai, Guangwei

    (Uni Press Hochschulschriften ; 107)

    1999  

    Author's details Guangwei Bai
    Series title Uni Press Hochschulschriften ; 107
    Keywords Leistungsbewertung ; Multimedia ; Verteiltes System ; Hochgeschwindigkeitsnetz ; Lastteilung ; Datenstrom
    Language English
    Size XII, 176 S, 21 cm
    Publisher Lit Verl
    Publishing place Hamburg
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Univ., FB Informatik, Diss.--Hamburg, 1999
    ISBN 3825842797 ; 9783825842796
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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